Overexpression of Transcription Factor Specificity Protein 1 (Sp1) Results in Centrosome Amplification

2021 ◽  
Author(s):  
Parin Himanshu Mehta
Keyword(s):  
Transcription Factor ◽  
Centrosome Amplification ◽  
Specificity Protein 1 ◽  
Factor Specificity ◽  
Specificity Protein

scholarly journals Thiamine transporter 2 is involved in high glucose-induced damage and altered thiamine availability in cell models of diabetic retinopathy

2019 ◽  
Vol 17 (1) ◽  
pp. 147916411987842 ◽  
Author(s):  
Elena Beltramo ◽  
Aurora Mazzeo ◽  
Tatiana Lopatina ◽  
Marina Trento ◽  
Massimo Porta
Keyword(s):  
Transcription Factor ◽  
Diabetic Retinopathy ◽  
High Glucose ◽  
Cell Models ◽  
Blood Retinal Barrier ◽  
Transketolase Activity ◽  
Specificity Protein 1 ◽  
Thiamine Transport ◽  
Factor Specificity ◽  
Specificity Protein

Thiamine prevents high glucose-induced damage in microvasculature, and progression of retinopathy and nephropathy in diabetic animals. Impaired thiamine availability causes renal damage in diabetic patients. Two single-nucleotide polymorphisms in SLC19A3 locus encoding for thiamine transporter 2 are associated with absent/minimal diabetic retinopathy and nephropathy despite long-term type 1 diabetes. We investigated the involvement of thiamine transporter 1 and thiamine transporter 2, and their transcription factor specificity protein 1, in high glucose-induced damage and altered thiamine availability in cells of the inner blood–retinal barrier. Human endothelial cells, pericytes and Müller cells were exposed to hyperglycaemic-like conditions and/or thiamine deficiency/over-supplementation in single/co-cultures. Expression and localization of thiamine transporter 1, thiamine transporter 2 and transcription factor specificity protein 1 were evaluated together with intracellular thiamine concentration, transketolase activity and permeability to thiamine. The effects of thiamine depletion on cell function (viability, apoptosis and migration) were also addressed. Thiamine transporter 2 and transcription factor specificity protein 1 expression were modulated by hyperglycaemic-like conditions. Transketolase activity, intracellular thiamine and permeability to thiamine were decreased in cells cultured in thiamine deficiency, and in pericytes in hyperglycaemic-like conditions. Thiamine depletion reduced cell viability and proliferation, while thiamine over-supplementation compensated for thiamine transporter 2 reduction by restoring thiamine uptake and transketolase activity. High glucose and reduced thiamine determine impairment in thiamine transport inside retinal cells and through the inner blood–retinal barrier. Thiamine transporter 2 modulation in our cell models suggests its major role in thiamine transport in retinal cells and its involvement in high glucose-induced damage and impaired thiamine availability.

Elevated Transcription Factor Specificity Protein 1 in Autistic Brains Alters the Expression of Autism Candidate Genes

2012 ◽  
Vol 71 (5) ◽  
pp. 410-418 ◽  
Author(s):  
Ismail Thanseem ◽  
Ayyappan Anitha ◽  
Kazuhiko Nakamura ◽  
Shiro Suda ◽  
Keiko Iwata ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Candidate Genes ◽  
Specificity Protein 1 ◽  
Factor Specificity ◽  
Specificity Protein
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