scholarly journals The Species Composition of Microbiota and Biological Properties of Dominant Bacterial Species in the Lacrimal Fluid in Patients with Endogenous Uveites

2021 ◽  
Vol 18 (4) ◽  
pp. 908-913
Author(s):  
A. Yu. Konkova ◽  
E. S. Horowitz ◽  
T. V. Gavrilova ◽  
M. V. Chereshneva
Keyword(s):  
Species Composition ◽  
Virulence Factors ◽  
Dominant Species ◽  
Biological Properties ◽  
Healthy Individuals ◽  
Coagulase Negative Staphylococci ◽  
Bacteriological Study ◽  
Lacrimal Fluid ◽  
Persistence Factors ◽  
Endogenous Uveitis

The purpose: to study the species composition of the lacrimal fluid microbiota in patients with endogenous uveitis and the biological properties of dominant species of bacteria.Patients and Methods. A bacteriological study of the lacrimal fluid of 107 patients (114 eyes) with endogenous uveitis and 28 practically healthy persons (control) was carried out. The research was performed using the conventional methods. The isolated microorganisms were identified up to the species. We studied the main biological properties of bacteria — the presence of persistence and virulence factors of isolated dominant species.The results. 43 strains of various types of bacteria were isolated from the lacrimal fluid of patients with uveitis, bacteria of the genus Staphylococcus prevailed, S. aureus was isolated in 23.3 % of cases, and the remaining strains were representatives of coagulase-negative staphylococci. The species composition of the bacteria isolated from practically healthy individuals did not differ in essence. Isolated strains of S. aureus had a significant set of virulence factors. All isolates lysed sheep red blood cells, 80 % of the cultures had lecithovitellase and 60 % — DNA-se activity. Representatives of coagulase-negative staphylococci were characterized only by hemolytic activity. In the study of persistence factors, it was found that all the studied strains of S. aureus and the vast majority of representatives of coagulase-negative staphylococci isolated from tears were resistant to lysozyme. Biofilm formation was more typical for S. aureus strains 90 % versus 34.4 % for representatives of coagulase-negative staphylococci (t = 2.194, p = 0.034). Similar patterns were found in relation to complement resistance, 90 % of S. aureus cultures and 37.5 % of coagulase-negative staphylococci (t = 2.055; p = 0.046).Conclusion. Among the microorganisms isolated from the lacrimal fluid, Staphylococcus bacteria prevailed, from which S. cohnii was most often isolated. S. aureus to a greater extent than coagulase-negative staphylococci had a certain set of virulence and persistence factors. The microbiocenosis of the lacrimal fluid of patients with endogenous uveitis did not differ from practically healthy individuals. The presence of S. aureus in lacrimal fluid is a risk factor for the development of endogenous uveitis in cases of chronic oral and ENT organ diseases.

Proposed virulence factors among coagulase-negative staphylococci isolated from two healthy populations

1988 ◽  
Vol 34 (7) ◽  
pp. 901-905 ◽  
Author(s):  
Larry M. Baddour ◽  
David L. Smalley ◽  
Michelle M. Hill ◽  
Gordon D. Christensen
Keyword(s):  
Antibiotic Resistance ◽  
Virulence Factors ◽  
Nosocomial Infections ◽  
Microtiter Plate ◽  
Multiple Antibiotic Resistance ◽  
Healthy Individuals ◽  
Coagulase Negative Staphylococci ◽  
Plate Method

Proposed virulence factors, including multiple antibiotic resistance and slime-mediated adherence, among coagulase-negative staphylococci colonizing healthy individuals from two different study populations were examined. Resistance to methicillin was more commonly seen than initially anticipated. In addition, adherence characteristics, as quantitated by a microtiter plate method, were very similar to those of strains of coagulase-negative staphylococci causing nosocomial infections.

scholarly journals Species diversity and biological properties of dominant species of causative agents of poststernotomic infectious complications in cardiac surgery

2021 ◽  
Vol 37 (6) ◽  
pp. 33-41
Author(s):  
A. V. Kasatov ◽  
E. S. Gorovits
Keyword(s):  
Cardiac Surgery ◽  
Dominant Species ◽  
Biological Properties ◽  
Infectious Complications ◽  
Bacteriological Study ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Causative Agents ◽  
Life Threatening ◽  
Wound Discharge

Objective. To study the microbial and biological properties of the dominant species of causative agents of infectious and inflammatory complications after cardiac surgery. Infectious and inflammatory complications (IIC) developing after cardiac surgery with sternal approach such as thoracic and rib osteomyelitis, sterno-mediastinitis are severe life threatening states. Materials and methods. Bacteriological study of the sternal wound discharge from 178 patients with different infectious and inflammatory complications after sternotomic open-heart surgeries was conducted. Biological properties of isolates were investigated. Results. There were detected 242 strains of microorganisms referring to different taxons. Gram-positive bacteria, in particular, S. aureus and S. epidermidis prevailed. Out of gram-negative bacteria, prevailed P. aeruginosa and E. coli. All isolates S. aureus and P. aeruginosa possessed different factors of virulence and persistence including ability to biofilm formation. Carbapenems displayed the greatest bactericidal activity in relation to S. aureus and P. aeruginosa. Conclusions. The obtained data permit to optimize the preoperative preparation of cardiac surgery patients.

Variation of Coagulase-Negative Staphylococci in the Skin Flora of Healthy Individuals during One Year

1998 ◽  
Vol 10 (2) ◽  
Author(s):  
K. Jung ◽  
A. Brauner ◽  
I. Kühn ◽  
J.-I. Flock ◽  
R. Möllby
Keyword(s):  
Healthy Individuals ◽  
Coagulase Negative Staphylococci ◽  
Skin Flora ◽  
One Year

scholarly journals Candida Albicans Virulence Factors and Its Pathogenicity

2021 ◽  
Vol 9 (4) ◽  
pp. 704
Author(s):  
Mariana Henriques ◽  
Sónia Silva
Keyword(s):  
Candida Albicans ◽  
Virulence Factors ◽  
Healthy Individuals ◽  
Mucosal Surfaces

Candida albicans lives as commensal on the skin and mucosal surfaces of the genital, intestinal, vaginal, urinary, and oral tracts of 80% of healthy individuals [...]

scholarly journals Virulence Factors in Coagulase-Negative Staphylococci

Pathogens ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 170
Author(s):  
Angela França ◽  
Vânia Gaio ◽  
Nathalie Lopes ◽  
Luís D. R. Melo
Keyword(s):  
Antibiotic Resistance ◽  
Virulence Factors ◽  
Resistance Genes ◽  
Biofilm Formation ◽  
Antibiotic Resistance Genes ◽  
Coagulase Negative Staphylococci ◽  
Healthcare Associated ◽  
Major Pathogens

Coagulase-negative staphylococci (CoNS) have emerged as major pathogens in healthcare-associated facilities, being S. epidermidis, S. haemolyticus and, more recently, S. lugdunensis, the most clinically relevant species. Despite being less virulent than the well-studied pathogen S. aureus, the number of CoNS strains sequenced is constantly increasing and, with that, the number of virulence factors identified in those strains. In this regard, biofilm formation is considered the most important. Besides virulence factors, the presence of several antibiotic-resistance genes identified in CoNS is worrisome and makes treatment very challenging. In this review, we analyzed the different aspects involved in CoNS virulence and their impact on health and food.

Spider mites of agricultural importance in China, with focus on species composition during the last decade (2008–2017) 

2018 ◽  
Vol 23 (11) ◽  
pp. 2087 ◽  
Author(s):  
Peng-Yu Jin ◽  
Lu Tian ◽  
Lei Chen ◽  
Xiao-Yue Hong
Keyword(s):  
Species Composition ◽  
Dominant Species ◽  
Integrated Management ◽  
Management Strategies ◽  
Spider Mites ◽  
Management Program ◽  
Pest Species ◽  
Panonychus Citri ◽  
Important Species ◽  
River Region

Understanding pest species composition and their geographic distribution of important spider mites is fundamental and indispensable to establish an integrated pest management program. From a long-term survey during 2008–2017 in mainland China, we found that Tetranychus truncatus was the most frequently sampled Tetranychus spider mite (48.5%), followed by T. pueraricola (21.2%), T. kanzawai (12.5%), T. urticae (red) (5.7%) and T. urticae (green) (4.5%). Among them, T. truncatus was the major mite pest in the north of China. T. kanzawai was the dominant species in the Middle and Lower Reaches of the Yangtze River Region and T. pueraricola was the most important species in the southwest region. Other common and serious pests include Amphitetranychus viennensis (6.8%) and Panonychus citri (3.8%). This pattern was largely different from that in 2002–2004, when T. urticae (green and red) was believed to be the most serious mite pest. The factors involved in the change of species composition are not clear and need more exploration. We suggested that the increasing corn planting range may be partly responsible for the conversion of dominant species from other spider mites to T. truncatus. Further research on the mechanisms underlying the change of dominant species will help develop integrated management strategies.

scholarly journals Composition and diversity of anurans in the Restinga of the Conde municipality, northern coast of the state of Bahia, northeastern Brazil

2016 ◽  
Vol 16 (3) ◽  
Author(s):  
Felipe Augusto Trindade Gondim-Silva ◽  
Alessandra Rodrigues Santos Andrade ◽  
Rafael Oliveira Abreu ◽  
Jamile Santos Nascimento ◽  
Geovane Paixão Corrêa ◽  
...  
Keyword(s):  
Species Richness ◽  
Species Diversity ◽  
Species Composition ◽  
Rainy Season ◽  
Dominant Species ◽  
The State ◽  
Northeastern Brazil ◽  
Freshwater Wetland ◽  
Northern Coast ◽  
Anuran Species

Abstract The Conde municipality is located in the northern coast of the state of Bahia (NC), northeastern Brazil, and is part of the Atlantic Tropical domain. The anuran fauna of the northern portion of the NC is still poorly known if compared to the southern portion. The Restinga is one of the predominant environments of the coastal plains of the NC and it is characterized essentially by presenting sandy soil covered by herbaceous and shrubby vegetation. The objective of this study was to determine the anuran species composition and diversity for the Restinga of the Conde municipality. Sampling was carried out at night by active search over four periods of five consecutive days each, two over the 'main rainy season' and two in a 'lesser rainy season', using 14 sample units (SUs) and five extra sample plots (EPs). We calculated dominance and species diversity using the Berger-Parker and Shannon-Wiener H' indices, respectively. We used accumulation curves and the Jackknife 1 estimator to estimate anuran species richness, considering only the data obtained from the SUs. We recorded 713 anuran specimens distributed within 33 species, 13 genera and five families (Bufonidae, Craugastoridae, Hylidae, Leptodactylidae and Microhylidae). The Hylidae and Leptodactylidae families had the highest species richness. Considering only the SUs (Jackknife 1 estimator in brackets), we recorded 28 species in the study area (33.9 ± 2.3), 13 in Shrubby Vegetation Zones - SVZ (20.8 ± 2.9) and 25 in Freshwater Wetland Zones - FWZ (28.9 ± 1.9). The abundance and species diversity of the FWZ (n = 638 specimens; H'= 2.4) were higher than those recorded for the SVZ (n = 52 specimens; H' = 1.9). The SVZ and FWZ showed distinct dominant species, wherein Pristimantis paulodutrai was the dominant species in SVZ and Scinax fuscomarginatus in FWZ. The Restinga of the Conde municipality stands out as the one with the highest anuran species richness already recorded considering only SVZ and FWZ. Moreover, its anuran species composition represented 55% of the anuran species known for the NC and included taxa common to three different morphoclimatic domains (Tropical Atlantic, Cerrado and Caatinga).

scholarly journals KERAGAMAN KOMPOSISI JENIS DAN STRUKTUR VEGETASI PADA KAWASAN HUTAN LINDUNG DENGAN POLA PHBM DI BKPH TAMPOMAS, KPH SUMEDANG, PERUM PERHUTANI DIVISI REGIONAL JAWA BARAT DAN BANTEN Species Composition and Vegetation Structure of Protected Forest Area using .

2017 ◽  
Vol 8 (2) ◽  
pp. 123-129
Author(s):  
Cecep Kusmana ◽  
Anggun Rahayu Melyanti
Keyword(s):  
Species Composition ◽  
Plant Species ◽  
Dominant Species ◽  
Forest Area ◽  
Stand Age ◽  
Protection Forest ◽  
Composition Structure ◽  
Forest Status ◽  
Coffee Species ◽  
Pinus Merkusii

BKPH Tampomas is part of the FMU area of Sumedang entirely classified into protected forest area. However, part of treated as CBFM with the aim of involfing the community in forest concervation activities the aim of this research is to describe and compare the structure and composition of the plant species between the subjected to CBFM and non CBFM Tampomas. The reaserch location was based on forest status of the protected area as having the same stand age and tree spacing. Data collection conducted throught the analysis of vegetation and soil sampling at each observation location species richness of BKPH Tampomas protection forest was relatively low either non CBFM of protected forest, CBFM protection forest of coffe and CBFM protection forest of cardamon. At the level of seedling stage there are 9 species and Calliandra haematocephala, the dominant. Species at the pole stage are found 3 species with Pinus merkusii species as the dominant species, at the tree stage are 2 species that dominated by Pinus merkusii. The covercrop found were 30 species and dominated by Lepturus repens. The low soil fertility and sandy soil texture composition also account for the low plant species in the BKPH Tampomas protection forest areas.Key words : CBFM cardamon, CDFM coffee, species composition, structure vegetation, Tampomas Mountain

scholarly journals SPECIES COMPOSITION OF FRESHWATER EELS (Anguilla spp.) IN EIGHT RIVERS FLOWING TO PALABUHANRATU BAY, SUKABUMI, INDONESIA

2016 ◽  
Vol 7 (2) ◽  
Author(s):  
Agus Alim Hakim ◽  
M Mukhlis Kamal ◽  
Nurlisa A Butet ◽  
Ridwan Affandi
Keyword(s):  
Cluster Analysis ◽  
Discriminant Analysis ◽  
Species Composition ◽  
Dominant Species ◽  
Morphological Identification ◽  
Morphometric Data ◽  
Freshwater Eel ◽  
Glass Eels ◽  
Freshwater Eels ◽  
Tropical Freshwater

Freshwater eel (Anguilla spp.) is a catadromus fish that migrates from freshwater into seawater for spawning and return to freshwater during larvae stage to grow. The objective of this study was to determine species composition of tropical freshwater eels based on morphology collected from several rivers flowing in to the Palabuhan Ratu Bay. Young, adult, and glass eels were caught in August 2014 until Desember 2014. Glass eels were only caught in Cimandiri River. Freshwater eels were classified by ano-dorsal length (AD) divided by total length (TL), cluster analysis, and discriminant analysis based on morphometric data. The results showed different AD/TL of young and adult, i.e., 1.93±0.51 for A. bicolor bicolor, and 16.99±0.65 for A. marmorata, and three different AD/TL of glass eels i.e.,1.72±1.05 for A. bicolor bicolor6.60±1.79 for A. nebulosa nebulosa, and 15.07±1.04 for A. marmorata. Cluster analysis showed three groups of population  characters of young and adult eels i.e.  A. bicolor bicolor (2 groups) and A. marmorata (1 group). Discriminant analysis showed three groups of glass eels population i.e., A. bicolor bicolor, A. nebulosa nebulosa, and A. marmorata. Morphological identification showed that young and adult eels in Cibareno  and Citepus rivers had two species i.e., A. bicolor bicolor and A. marmorata. Glass eels from Cimandiri river had three species i.e., A. bicolorbicolor, A. nebulosanebulosa, and A. marmorata. A. bicolor bicolor was found in each rivers and as a dominant species in Palabuhan Ratu Bay.Keywords: A. bicolor bicolor, A. marmorata, A. nebulosa nebulosa, species composition

scholarly journals Immunological aspects of bacterial keratites in patients with diabetes mellitus

2021 ◽  
Vol 9 (2) ◽  
pp. 6-9
Author(s):  
O.V. Zavoloka ◽  
P.A. Bezditko ◽  
L.P. Abramova ◽  
V.O. Vekshyn
Keyword(s):  
Diabetes Mellitus ◽  
Anterior Segment ◽  
Enzyme Linked Immunosorbent Assay ◽  
Bacterial Keratitis ◽  
Healthy Individuals ◽  
Lacrimal Fluid ◽  
Cytokine Balance ◽  
Patients With Diabetes ◽  
Contralateral Eye ◽  
Type 1 Dm

Background. The purpose was to analyze the cytokine balance of lacrimal fluid in patients with bacterial keratitis and diabetes mellitus (DM) at the first visit and to identify the immunological aspects of the disease. Materials and methods. The analysis of pro- and anti-inflammatory cytokine concentration in the lacrimal fluid was performed in 17 patients with type 1 DM and bacterial keratitis and 15 nondiabetic patients with bacterial keratitis at the first visit. Data from 14 healthy individuals were used for comparison. In addition to standard ones, ophthalmic examination methods included bacteriological examination, fluorescein test, anterior segment optical coherence tomography, non-contact corneal aesthesiometry. The levels of interleukin (IL) 1β, IL-6 and IL-10 in the lacrimal fluid of the sick and the contralateral eye were determined by a quantitative colorimetric enzyme-linked immunosorbent assay using ELISA kits. Results. In DM patients with bacterial keratitis, the concentration of IL-1β and IL-6 in the lacrimal fluid of the sick eye exceeded that in healthy individuals (p < 0.05) and did not differ significantly from nondiabetic patients with bacterial keratitis (p > 0.05). In the lacrimal fluid of the contralateral eye of DM patients with bacterial keratitis, the level of IL-1β and IL-6 exceeded the corresponding indicators of nondiabetic patients with bacterial keratitis and healthy individuals (p < 0.05). The concentration of IL-10 in the lacrimal fluid of the contralateral eye in DM patients with bacterial keratitis exceeded that in healthy individuals (p < 0.05) and did not significantly differ from those in nondiabetic patients with bacterial keratitis (p > 0.05). Conclusions. DM patients with bacterial keratitis have immunological features of the disease.

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