scholarly journals The Effect of Endurance Training Intensity on the Expression of Perlipin a Protein of Subcutaneous Adipose Tissue and Pancreatic B cells Function in Diabetic Rats

2020 ◽  
Author(s):  
Hadi Ghaedi
Keyword(s):  
B Cells ◽  
Endurance Training ◽  
High Intensity ◽  
Subcutaneous Adipose Tissue ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
P Value ◽  
Low Intensity ◽  
Pancreatic B Cells ◽  
Subcutaneous Adipose

Objective: The aim of this study was to compare the three endurance training intensities on the expression of Perlipin A protein in subcutaneous adipose tissue and pancreatic B-cells function in male diabetic rats. Materials and Methods: In this study, 40 healthy male wistar rats were divided into five groups, including diabetic with low intensity endurance training, diabetic with moderate intensity endurance training, diabetic with high intensity endurance training, diabetic control and healthy control. After diabetic induction with streptozotocin, endurance training was performed with low intensity, moderate and severe for eight weeks, three sessions per week. The relative expression of Perilipin A was measured by western blot technique. Results: The results indicated a significant effect of endurance training with three intensities on serum levels of insulin and glucose and pancreatic B-cells function ( P -value: 0.001). Also the results showed that there was no significant difference between Perlipin A expression in healthy and diabetic control groups with endurance training groups (with low, moderate and high intensity) ( P -value: 0.07). Conclusion: However, Moderate and high intensity endurance training compared to low-intensity training can compensate for the loss caused by diabetes in the expression of the Perlipin A protein but the difference was not significant. It seems that more intensity endurance training lead to more increase in Perlipin A expression in diabetic rats.

scholarly journals The Effects of Continuous and High Intensity Interval Trainings on Plasma Betatrophin Level in Diabetic Rats Treated with Metformin

2020 ◽  
Author(s):  
Yadollah Mahmoudi ◽  
Mandana Gholami ◽  
Hojatolah Nikbakht ◽  
Khosrow Ebrahim ◽  
Salar Bakhtiyari
Keyword(s):  
Blood Glucose ◽  
High Intensity ◽  
Training Session ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Moderate Intensity ◽  
Control Group ◽  
P Value ◽  
Continuous Training ◽  
High Intensity Interval

Objective: The study aimed to determine the effect of eight weeks high intensity interval (HIIT) and sub-maximal continuous trainings on plasma betatrophin level in diabetic rats treated with metformin. Materials and Methods: In this experimental study, 42 diabeticwistar rats were divided into six groups (n=7): diabetic control (C), diabetic control + metformin consumption(C+M), diabetic HIIT, diabetic HIIT + metformin (HIIT+M), diabetic sub-maximal continuous training (SMCT), and diabetic sub-maximal continuous training + metformin (SMCT+M). Metformin was given 150 mg/kg/day by gavage every day, 48 hours after the end of the last training session, the rats were sacrificed. Then blood glucose and glycated hemoglobin (HbA1c) levels were measured. One-way ANOVA test was used for statistical analysis of data. Results: The level of plasma betatrophin was significantly different in the HIIT ( P -value= 0.01) and C+M ( P -value= 0.001) groups compared to C group. Blood glucose was significantly decreased in all training groups with or without betatrophin compared with the diabetic control group ( P -value= 0.001). However, there were no significant changes between glucose levels in HIIT, HIIT+M, SMCT, and SMCT+M groups but SMCT showed most reduction in blood glucose. Conclusion: Treatment with metformin did not change blood glucose but two types of exercise training with high and moderate intensity reduced blood glucose thus exercise can be a good alternative modality rather than taking medicine.

scholarly journals The Effect of High Intensity Aerobic Exercise on Levels of Galectin-3 and Protein Kinase C in Diabetic Male Rats

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Abolfazl Samiei ◽  
Naser Behpoor ◽  
Vahid Tadibi ◽  
Rozita Fathi
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Endurance Training ◽  
High Intensity ◽  
Cardiac Fibrosis ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Kinase C ◽  
Galectin 3

Background: Cardiac fibrosis is one of the complications of diabetes. Physical activity may be effective in reducing cardiac fibrosis through affecting hyperglycemia. Objectives: Accordingly, the purpose of this study was to investigate the effect of high intensity aerobic exercise on levels of galectin-3 and protein kinase C in diabetic rats. Methods: In this study, 30 male wistar rats were randomly divided into 3 groups (control, diabetic control and exercise diabetic). The induction of diabetes was performed by intraperitoneally injected streptozotocin solution at a dose of 55 mg/kg of body weight. The training program included 8 weeks of treadmill running for 60 minutes and a speed of 34 m/min. Subjects in all experimental groups (48 hours after the last training session) were anesthetized and then the heart tissue was isolated from the aortic root area. Data analysis was performed using one-way ANOVA and Tukey’s post hoc test to determine the difference between the groups. Results: The results showed that galectin-3 and PKC had a significant increase in the diabetics control group compared to the healthy group. It was also found that endurance training with high intensity caused a significant decrease in galectin-3 and PKC compared to diabetic control group. Conclusions: It seems that endurance training in diabetes is an effective method for decreasing the activity of signaling cascade of the pathway of pathogenesis of fibrosis in the heart of diabetic rats.

scholarly journals Response of Mitochondrial Respiration in Adipose Tissue and Muscle to 8 Weeks of Endurance Exercise in Obese Subjects

2020 ◽  
Vol 105 (11) ◽  
Author(s):  
Christoph Hoffmann ◽  
Patrick Schneeweiss ◽  
Elko Randrianarisoa ◽  
Günter Schnauder ◽  
Lisa Kappler ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Adipose Tissue ◽  
Insulin Sensitivity ◽  
Endurance Training ◽  
Mitochondrial Respiration ◽  
Subcutaneous Adipose Tissue ◽  
Muscle Fibers ◽  
Sensitivity Index ◽  
Respiratory Capacity ◽  
Subcutaneous Adipose

Abstract Context Exercise training improves glycemic control and increases mitochondrial content and respiration capacity in skeletal muscle. Rodent studies suggest that training increases mitochondrial respiration in adipose tissue. Objective To assess the effects of endurance training on respiratory capacities of human skeletal muscle and abdominal subcutaneous adipose tissue and to study the correlation with improvement in insulin sensitivity. Design Using high-resolution respirometry, we analyzed biopsies from 25 sedentary (VO2 peak 25.1 ± 4.0 VO2 mL/[kg*min]) subjects (16 female, 9 male; 29.8 ± 8.4 years) with obesity (body mass index [BMI] 31.5 ± 4.3 kg/m2), who did not have diabetes. They performed a supervised endurance training over 8 weeks (3 × 1 hour/week at 80% VO2 peak). Results Based on change in insulin sensitivity after intervention (using the Matsuda insulin sensitivity index [ISIMats]), subjects were grouped in subgroups as responders (>15% increase in ISIMats) and low-responders. The response in ISIMats was correlated to a reduction of subcutaneous and visceral adipose tissue volume. Both groups exhibited similar increases in fitness, respiratory capacity, and abundance of mitochondrial enzymes in skeletal muscle fibers. Respiratory capacities in subcutaneous adipose tissue were not altered by the intervention. Compared with muscle fibers, adipose tissue respiration showed a preference for β-oxidation and complex II substrates. Respiratory capacities were higher in adipose tissue from female participants. Conclusion Our data show that the improvement of peripheral insulin sensitivity after endurance training is not directly related to an increase in mitochondrial respiratory capacities in skeletal muscle and occurs without an increase in the respiratory capacity of subcutaneous adipose tissue.

Different skeletal muscle HSP70 responses to high-intensity strength training and low-intensity endurance training

2004 ◽  
Vol 91 (2-3) ◽  
pp. 330-335 ◽  
Author(s):  
Yuefei Liu ◽  
Werner Lormes ◽  
Liangli Wang ◽  
Susanne Reissnecker ◽  
J�rgen M. Steinacker
Keyword(s):  
Skeletal Muscle ◽  
Strength Training ◽  
Endurance Training ◽  
High Intensity ◽  
Low Intensity

scholarly journals Effect of the Combination of Training and ERRα Inhibition on Liver Metabolism by Modulation of PDK4 and LXR-α Expression in STZ-Induced Diabetic and Healthy Rats

2020 ◽  
Vol 10 (6) ◽  
pp. 7011-7022 ◽  
Keyword(s):  
Exercise Training ◽  
Endurance Training ◽  
Dietary Habits ◽  
Metabolic Diseases ◽  
Life Quality ◽  
Public Health Problem ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Control Group ◽  
Male Wistar Rats

Diabetes is a public health problem that affects life quality. Exercise training (ET) and controlled dietary habits improve metabolic diseases such as diabetes. The mechanisms by which exercise training ameliorate metabolic diseases are not fully clear. We designed the current study to evaluate the combination of ERRα suppression and ET effects on the expression of LXR-α, PDK4, and PPARα in healthy and STZ-induced diabetic rats. Fifty-six male Wistar rats were divided into 8 groups (n = 7) as follows; control, diabetic control (a single dose of 45 mg/kg of STZ), ERRα inhibition group (received 0.48 mg/kg of XCT790), endurance training, diabetic rats which received XCT790, diabetic rats which performed endurance training, rats which received XCT790 and performed endurance training, and diabetic rats which received XCT790 and also performed endurance training. Expression of the target gene and protein was carried out on the liver tissue. Our results showed that ET significantly increased PDK4, PPARα, and ERRα expression. ERRα suppression significantly increased LXR-α and PDK4 expression in healthy rats compared to the healthy control group. In the diabetic group with ERRα suppression, LXR-α expression significantly upregulated. The combination of ET and ERRα suppression did not change LXR-α expression compared to healthy and diabetic groups (CTL/ERR), but the expression of PDK4, PPARα, and ERRα was significantly upregulated.

Effects of Increased Load of Low- Versus High-Intensity Endurance Training on Performance and Physiological Adaptations in Endurance Athletes

2021 ◽  
pp. 1-10
Author(s):  
Rune K. Talsnes ◽  
Roland van den Tillaar ◽  
Øyvind Sandbakk
Keyword(s):  
Oxygen Uptake ◽  
Endurance Training ◽  
Maximal Oxygen Uptake ◽  
High Intensity ◽  
Time Trial ◽  
Endurance Athletes ◽  
High Intensity Training ◽  
Low Intensity ◽  
Physiological Adaptations ◽  
Intensity Training

Purpose: To compare the effects of increased load of low- versus high-intensity endurance training on performance and physiological adaptations in well-trained endurance athletes. Methods: Following an 8-week preintervention period, 51 (36 men and 15 women) junior cross-country skiers and biathletes were randomly allocated into a low-intensity (LIG, n = 26) or high-intensity training group (HIG, n = 25) for an 8-week intervention period, load balanced using the overall training impulse score. Both groups performed an uphill running time trial and were assessed for laboratory performance and physiological profiling in treadmill running and roller-ski skating preintervention and postintervention. Results: Preintervention to postintervention changes in running time trial did not differ between groups (P = .44), with significant improvements in HIG (−2.3% [3.2%], P = .01) but not in LIG (−1.5% [2.9%], P = .20). There were no differences between groups in peak speed changes when incremental running and roller-ski skating to exhaustion (P = .30 and P = .20, respectively), with both modes being significantly improved in HIG (2.2% [3.1%] and 2.5% [3.4%], both P < .01) and in roller-ski skating for LIG (1.5% [2.4%], P < .01). There was a between-group difference in running maximal oxygen uptake changes (P = .04), tending to improve in HIG (3.0% [6.4%], P = .09) but not in LIG (−0.7% [4.6%], P = .25). Changes in roller-ski skating peak oxygen uptake differed between groups (P = .02), with significant improvements in HIG (3.6% [5.4%], P = .01) but not in LIG (−0.1% [0.17%], P = .62). Conclusion: There was no significant difference in performance adaptations between increased load of low- versus high-intensity training in well-trained endurance athletes, although both methods improved performance. However, increased load of high-intensity training elicited better maximal oxygen uptake adaptations compared to increased load of low-intensity training.

Does Polarized Training Improve Performance in Recreational Runners?

2014 ◽  
Vol 9 (2) ◽  
pp. 265-272 ◽  
Author(s):  
Iker Muñoz ◽  
Stephen Seiler ◽  
Javier Bautista ◽  
Javier España ◽  
Eneko Larumbe ◽  
...  
Keyword(s):  
Training Program ◽  
Endurance Training ◽  
High Intensity ◽  
Ventilatory Threshold ◽  
Training Session ◽  
Moderate Intensity ◽  
Intervention Period ◽  
Low Intensity ◽  
Recreational Runners ◽  
The Impact

Purpose:To quantify the impact of training-intensity distribution on 10K performance in recreational athletes.Methods:30 endurance runners were randomly assigned to a training program emphasizing low-intensity, sub-ventilatory-threshold (VT), polarized endurance-training distribution (PET) or a moderately high-intensity (between-thresholds) endurance-training program (BThET). Before the study, the subjects performed a maximal exercise test to determine VT and respiratory-compensation threshold (RCT), which allowed training to be controlled based on heart rate during each training session over the 10-wk intervention period. Subjects performed a 10-km race on the same course before and after the intervention period. Training was quantified based on the cumulative time spent in 3 intensity zones: zone 1 (low intensity, <VT), zone 2 (moderate intensity, between VT and RCT), and zone 3 (high intensity, >RCT). The contribution of total training time in each zone was controlled to have more low-intensity training in PET (±77/3/20), whereas for BThET the distribution was higher in zone 2 and lower in zone 1 (±46/35/19).Results:Both groups significantly improved their 10K time (39min18s ± 4min54s vs 37min19s ± 4min42s, P < .0001 for PET; 39min24s ± 3min54s vs 38min0s ± 4min24s, P < .001 for BThET). Improvements were 5.0% vs 3.6%, ~41 s difference at post-training-intervention. This difference was not significant. However, a subset analysis comparing the 12 runners who actually performed the most PET (n = 6) and BThET (n = 16) distributions showed greater improvement in PET by 1.29 standardized Cohen effect-size units (90% CI 0.31–2.27, P = .038).Conclusions:Polarized training can stimulate greater training effects than between-thresholds training in recreational runners.

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