scholarly journals The effects of supplemented sericin on in vitro maturation and preimplantation development of mouse embryos: An experimental study

2021 ◽  
pp. 921-928
Author(s):  
Omid Banafshi ◽  
Sherko Nasseri ◽  
Leila Farhadi ◽  
Masoud Alasvand ◽  
Mohammad Bagher Khadem-Erfan ◽  
...  
Keyword(s):  
Embryo Culture ◽  
In Vitro Maturation ◽  
Culture Medium ◽  
Control Group ◽  
Vitro Fertilization ◽  
In Vitro Development ◽  
Embryo Culture Medium ◽  
Vitro Development

Background: Mouse embryo culture condition is an essential part of transgenic, reproductive and developmental biology laboratories. Mouse embryonic culture media may have a high risk of serum contamination with pathogens.  Objective: To investigate the effect of sericin as an embryo culture medium supplement on in vitro maturation (IVM), in vitro fertilization (IVF), and development of the preimplantation embryo in mice. Materials and Methods: The effects of sericin at three concentrations (subgroups) of 0.1%, 0.5%, and 1% as a medium supplement on IVM, IVF, and in vitro development of mouse embryos were separately investigated and compared with a sericin-free (control) group. The cumulative effect of the three concentrations was evaluated for IVM + in vitro development and IVF + in vitro development as follow-up groups. Results: In the IVM group, compared to the control group, the number of oocysts reaching the MII stage was significantly higher when 1% sericin was used (161/208 = 77.4%). No significant results were observed in the IVF and in vitro development groups with different concentrations of sericin compared to the control group. Among the follow-up groups, in the IVM + in vitro development group, the number of oocytes was higher after passing the IVM and IVF and reaching the blastocysts stage when 1% sericin was used, compared with other sericin subgroups. A significant difference was also noted when compared with the control group (p = 0.048). The IVF + in vitro development study group, on the other hand, did not show any significant relationship. Conclusion: It can be concluded that 1% sericin can be used as a supplement in mouse embryo cultures to improve the IVM rate. Also, based on the findings, sericin appears to be an effective supplement which can have a positive effect on the development of embryos derived from IVM. Key words: Sericin, In vitro maturation, In vitro fertilization, Preimplantation embryo, Culture medium, Mice.

Effect of LH and Estradiol-17β Supplementation at Different Time Points on In vitro Development of Preantral Follicles in Sheep

2020 ◽  
Author(s):  
L.S.S. Varaprasad Reddy ◽  
B.R. Naik ◽  
A.V.N. Sivakumar ◽  
B. Punyakumari ◽  
J. Suresh
Keyword(s):  
In Vitro Maturation ◽  
Culture Medium ◽  
Follicular Development ◽  
Standard Medium ◽  
In Vitro Development ◽  
Preantral Follicles ◽  
Time Points ◽  
Vitro Development ◽  
Estradiol 17Β

Background: Ovarian follicular development and growth are controlled by many hormones and growth factors. Despite the fact that LH and estradiol-17β have been utilized for the in vitro culture of preantral follicles yet, the suitable time points of supplementation of LH and estradiol-17β is not known. Therefore this study aimed to investigate the influence of addition of LH and estradiol-17β at different time points on in vitro development of preantral follicles (PFs’) in sheep. Method: Preantral follicles isolated from the ovarian cortical slices using micro dissection method were cultured for six days in Bicarbonate buffered Tissue culture medium 199B (TCM 199B) or in a standard culture medium supplemented with LH (2 μg/ml) and estradiol-17β (5 ng/ml) at different points during the culture period. COCs isolated from the follicles at the end of six day culture in different treatments were subjected to in vitro maturation for additional 24h. Result: Supplementation of LH and estradiol-17β during last two days of the culture supported better proportion of PFs’ exhibiting growth whereas supplementation of LH and estradiol-17β during first two days of the culture supported better average increase in diameter and proportion of PFs’ exhibiting antrum formation at the end of six day culture. Further the oocytes in COCs isolated at the end of culture in these treatments and subsequently subjected to in vitro maturation (IVM) for 24hr developed at a higher frequency to MII (metaphase II) stage. Supplementation of LH and estradiol-17β to TCM 199B culture medium in early stages followed by standard medium alone in later stages supports better development of PFs’ in vitro. Following supplementation with LH and estradiol-17β for the first two days culture of PFs’ in standard medium appears to be advantageous for the development of preantral follicles in vitro.

Isolation of a histamine releasing factor from human embryo culture medium after in-vitro fertilization

1987 ◽  
Vol 2 (4) ◽  
pp. 341-344 ◽  
Author(s):  
Roberta Cocchiara ◽  
Giovana Di Trapani ◽  
Antonina Azzolina ◽  
Giuseppe Albeggiani ◽  
Rosanna Ciriminna ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Embryo Culture ◽  
Human Embryo ◽  
Culture Medium ◽  
Vitro Fertilization ◽  
Embryo Culture Medium ◽  
Human Embryo Culture

scholarly journals LIF level is a predictive marker for clinical pregnancy following IVF-ET of patients with fallopian tube obstruction

2020 ◽  
Author(s):  
Zewu Li ◽  
Ruimei Li ◽  
Huiying Dai ◽  
Xiaoyun Li ◽  
Xiao Han ◽  
...  
Keyword(s):  
Pregnancy Outcome ◽  
Embryo Culture ◽  
Culture Medium ◽  
Predictive Marker ◽  
Clinical Pregnancy ◽  
Vitro Fertilization ◽  
Embryo Culture Medium ◽  
Infertile Patients ◽  
Ivf Et

Abstract Background: Leukemia inhibitory factor (LIF) has played a vital role in a series of reproductive events, including follicle growth, embryo growth and differentiation. However, it is unclear whether the level of LIF in embryo culture medium can be used as a marker for clinical pregnancy. In this study, we aimed to investigate whether LIF level in embryo culture medium can act as a predictive marker for pregnancy outcome of in vitro fertilization-embryo transfer (IVF-ET) in infertile patients due to tubal problems.Methods: A total of 104 infertile patients due to tubal problems underwent IVF-ET treatment. The patients were divided into two groups according to whether they were clinically pregnant. The level of LIF in the embryo culture medium was measured, and the correlation between LIF level and embryo quality and clinical pregnancy outcome was analyzed. The embryo culture medium was collected on the day of blastocyst transplantation.Results: Compared to non-pregnant group, LIF level in the embryo culture medium on the day of blastocyst transplantation was significantly higher in the pregnant group.Conclusions: LIF level in the embryo culture medium may be used as a non-invasive auxiliary biomarker for predictive clinical pregnancy in infertile patients with tubal problems that using single blastocyst transfer method.

scholarly journals Does Embryo Culture Medium Influence the Health and Development of Children Born after In Vitro Fertilization?

PLoS ONE ◽  
2016 ◽  
Vol 11 (3) ◽  
pp. e0150857 ◽  
Author(s):  
Céline Bouillon ◽  
Roger Léandri ◽  
Laurent Desch ◽  
Alexandra Ernst ◽  
Céline Bruno ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Embryo Culture ◽  
Culture Medium ◽  
Vitro Fertilization ◽  
Embryo Culture Medium ◽  
Health And Development ◽  
Medium Influence

scholarly journals LIF level is a predictive marker for clinical pregnancy following IVF-ET of patients with fallopian tube obstruction

2020 ◽  
Author(s):  
Zewu Li ◽  
Ruimei Li ◽  
Huiying Dai ◽  
Xiaoyun Li ◽  
Xiao Han ◽  
...  
Keyword(s):  
Pregnancy Outcome ◽  
Embryo Culture ◽  
Culture Medium ◽  
Predictive Marker ◽  
Clinical Pregnancy ◽  
Vitro Fertilization ◽  
Embryo Culture Medium ◽  
Infertile Patients ◽  
Ivf Et

Abstract Background: Leukemia inhibitory factor (LIF) has played a vital role in a series of reproductive events, including follicle growth, embryo growth and differentiation. However, it is unclear whether the level of LIF in embryo culture medium can be used as a marker for clinical pregnancy. In this study, we aimed to investigate whether LIF level in embryo culture medium can act as a predictive marker for pregnancy outcome of in vitro fertilization-embryo transfer (IVF-ET) in infertile patients due to tubal problems.Methods: A total of 104 infertile patients due to tubal problems underwent IVF-ET treatment. The patients were divided into two groups according to whether they were clinically pregnant. The level of LIF in the embryo culture medium was measured, and the correlation between LIF level and embryo quality and clinical pregnancy outcome was analyzed. The embryo culture medium was collected on the day of blastocyst transplantation.Results: Compared to non-pregnant group, LIF level in the embryo culture medium on the day of blastocyst transplantation was significantly higher in the pregnant group.Conclusions: LIF level in the embryo culture medium may be used as a non-invasive auxiliary biomarker for predictive clinical pregnancy in infertile patients with tubal problems that using single blastocyst transfer method.

scholarly journals Influence of embryo culture medium on incidence of ectopic pregnancy in in vitro fertilization

2015 ◽  
Vol 104 (6) ◽  
pp. 1442-1445 ◽  
Author(s):  
Shengli Lin ◽  
Rong Li ◽  
Xiaoying Zheng ◽  
Hongbin Chi ◽  
Xiulian Ren ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Ectopic Pregnancy ◽  
Embryo Culture ◽  
Culture Medium ◽  
Vitro Fertilization ◽  
Embryo Culture Medium
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