scholarly journals SEX DETERMINATION OF BALI STARLING (Leucopsar rothschildi) USING MOLECULAR SEXING

2015 ◽  
Vol 2 (1) ◽  
pp. 114
Author(s):  
Putu Indra Pramana Wirastika ◽  
Ignatius Pramana Yuda ◽  
Felicia Zahida
Keyword(s):  
Dna Binding ◽  
Sex Determination ◽  
Gel Electrophoresis ◽  
Morphological Characters ◽  
Molecular Sexing ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Pcr Products ◽  
Conventional Methods

<p>Bali Starling (Leucopsar rothschildi) are monomorphic at the age of nestling. For the conservation of bird it important is to determine its sex at the earlier stage. Conventional methods have limitations. This study applied PCR-based molecular sexing to answer this issue. This study aimed to obtain the most effective molecular primers to identify the sex of Bali starling. The most common used combination of P2/P8, 2550F/ 2718R and 1237L/1272H primers, which amplify CHD1 gene (Chromo-helicase-DNA-binding) were evaluated. DNA samples were obtained from secondary wing feathers of young Bali Starling. Separation in agarose gel electrophoresis of PCR products showed that the three primers were successfully amplified the samples with different degrees of success, that was 90% (P2/P8), 86.7% (2550F/2718R), and 73.3% (1237L/1272H), respectively. However, only the combination of P2/P4 and 2550F/2718R primers was able to sex Bali Starling based on observation of PCR products on agarose gel. The sizes of the genes were slightly different with those reported on previous studies. Most of the results of molecular sexing were in accordance with the sex based on morphological characters.</p><p><br /><strong>Keywords</strong> : Bali starling, Leucopsar rothschildi, molecular sexing, CHD gene</p>

Varieties of Human Serum Lipoprotein Pattern: Evaluation by Agarose Gel Electrophoresis

1971 ◽  
Vol 17 (5) ◽  
pp. 427-429 ◽  
Author(s):  
N M Papadopoulos ◽  
J A Kintzios
Keyword(s):  
Human Serum ◽  
Gel Electrophoresis ◽  
Clinical Laboratory ◽  
Serum Lipoprotein ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Practical Applications ◽  
Electrophoretic Technique ◽  
Lipoprotein Pattern

Abstract A previously reported agarose gel electrophoretic technique for the determination of serum lipoprotein patterns has been modified for analysis of a large number of samples for screening and epidemiological purposes. In addition, we demonstrate the varieties of lipoprotein patterns that can clearly be distinguished and visually evaluated for practical applications in the clinical laboratory.

Determination of triglycerides in lipoproteins separated by agarose gel electrophoresis

1995 ◽  
Vol 115 ◽  
pp. S118 ◽  
Author(s):  
K. Winkler ◽  
M. Nauck ◽  
R. Siekmeier ◽  
W. März ◽  
H. Wieland
Keyword(s):  
Gel Electrophoresis ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis

scholarly journals Genetic variability of broodstocks of restocking programs in Brazil

2015 ◽  
pp. 4677-4687 ◽  
Author(s):  
Nelson Lopera-Barrero ◽  
Ed Lima ◽  
Luiz Filho ◽  
Elenice Goes ◽  
Pedro Castro ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Variability ◽  
Gel Electrophoresis ◽  
Shannon Index ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
General Management ◽  
Rhamdia Quelen ◽  
Pcr Products ◽  
Salminus Brasiliensis

ABSTRACT Objective. The aim of this study was evaluate the genetic diversity of the following broodstocks: piapara (Leporinus elongatus), dourado (Salminus brasiliensis), jundiá (Rhamdia quelen) and cachara (Pseudoplatystoma fasciatum) already useful for restocking programs in the Paranapanema, Iguaçu and Paraná Brazilian Rivers. Materials and methods. Samples from the caudal fin of 122 fish were analyzed. DNA was extracted by NaCl protocol. PCR products were separated by a horizontal agarose gel electrophoresis. The fragments were visualized by staining with ethidium bromide. Results. The amplification of 25 primers generated different fragments in studied species that allowed characterizing 440 fragments of 100-2900 bp. High percentage of polymorphic fragments (66.67 to 86.29), Shannon index (0.365 to 0.486) and genetic diversity of Nei (0.248 to 0.331) were detected. Conclusions. The level of genetic variability in the broodstocks was adequate for allowing their use in restocking programs in the studied Rivers. However, periodical monitoring studies of genetic variability in these stocks, the mating system, reproductive system and general management must be made to guarantee the preservation of wild populations.RESUMEN Objetivo. El objetivo de este estudio fue evaluar la diversidad genética de los siguientes lotes de reproductores: piapara (Leporinus elongatus), dourado (Salminus brasiliensis), jundiá (Rhamdia quelen) y cachara (Pseudoplatystoma fasciatum) utilizados para programas de repoblación en los ríos brasileños Paranapanema, Iguaçu y Paraná. Materiales y métodos. Muestras de aleta caudal de 122 peces fueron analizadas. El ADN fue extraído por el protocolo de NaCl. Los productos de PCR fueron separados por electroforesis horizontal en gel de agarosa. Los fragmentos fueron visualizados por marcación con bromuro de etidio. Resultados. La amplificación de los 25 iniciadores produjo diferentes fragmentos en las especies estudiadas que permitieron caracterizar 440 fragmentos de 100 a 2900 pb. Fueron detectados un alto porcentaje de fragmentos polimórficos (66.67 a 86.29), de índice de Shannon (0.365 a 0.486) y de diversidad genética de Nei (0.248 a 0.331). Conclusiones. El nivel de variabilidad genética en los lotes de reproductores fue adecuado para su utilización en programas de repoblación en los ríos estudiados. Sin embargo, estudios de monitoreo periódico de la variabilidad genética en esos lotes, del sistema de cruzamiento, del sistema reproductivo y del manejo general deben ser realizados para garantizar la preservación de las populaciones naturales. 

scholarly journals Unified Procedure for the Determination of Antibiotics in Animal Feeds

1993 ◽  
Vol 76 (3) ◽  
pp. 514-525 ◽  
Author(s):  
Michael J Salvatore ◽  
Stanley E Katz
Keyword(s):  
Gel Electrophoresis ◽  
Quantitative Method ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Qualitative And Quantitative ◽  
Animal Feeds ◽  
Agar Diffusion Assay ◽  
The Individual ◽  
Diffusion Assay

Abstract A new method was developed for the determination of antibiotics in animal feeds. This qualitative and quantitative method can be applied to 17 antibiotics currently accepted for use in animal feeds. A solvent prewash step is used to differentiate groups of antibiotics and individuals of the same class of antibiotics and to remove interfering substances. Agarose gel electrophoresis at pH 6.0 and 8.0 is used to further differentiate the individual antibiotics and as an agar diffusion assay. Minimum detectable concentrations of antibiotics currently accepted for use in animal feeds were determined against selected microorganisms.

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