Selection and characterization of potential probiotic Lactobacilli spp isolated from chicken feces may be used as a potent antibacterial agent

2016 ◽  
Vol 35 (1) ◽  
Author(s):  
Nazila Aazami ◽  
Enayatollah Kalantar ◽  
Helen Poormazaheri ◽  
Nassimeh Setayesh vali Pour ◽  
Gholamreza Salehi Jouzan
Keyword(s):  
Antibacterial Activity ◽  
Antibiotic Resistance ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Acid Tolerance ◽  
Resistance Test ◽  
Effect Of Temperature ◽  
Rdna Sequencing

<italic>Lactobacillus</italic> strains commonly used as probiotics which possess some properties such as resistance to gastric acidity and bile salts, production of antimicrobial compounds, the ability to modulate immune responses, and adhesion to gut tissues. This study aimed to isolate, identify, production and characterization of the probiotic strains. Total of 10 digestive tracts of chickens fed without antibiotics for presence of <italic>Lactobacillus spp</italic> was evaluated. All isolates were grown with different conditions like effect of temperature, pH, pepsine, bile, sodium chloride tolerance, aggregation, adhesion and antibiotic resistance test tested and then antibacterial activity was determined.In this study, 34 lactic acid bacteria were investigated as putative probiotic candidates. Based on the molecular identification using 16S rDNA sequencing the isolates belonged to <italic>Lactobacillus</italic> species. The strains showed potent antibacterial activity. The results on acid tolerance showed that all the strains tested survived at pH 2.0 to 5.5. <italic>L. agilis</italic> strain M18, <italic>L. salivarius</italic> strain M10, <italic>L.vaginalis</italic> strains M8 and M19, and <italic>L. crispatus</italic> strains Or2 and M10 showed the lowest sensitivity to pepsine. All the studied Lactobacillus strains were able to grow at both 15 and 45°C . Five Lactobacillus strains adhered well to the Caco-2 cells. The result of antibiotic resistance test of 34 lactic acid bacteria showed that most of them were resistant or intermediate. Based on 34 <italic>Lactobacillus</italic> strains, <italic>L. vaginalis</italic> strain M8 and <italic>L. reuteri</italic> strain M6 were selected for the further in vivo assays and possible eventual use as prophylactic and therapeutic agent.

scholarly journals Transfer of Antibiotic Resistance Marker Genes between Lactic Acid Bacteria in Model Rumen and Plant Environments

2009 ◽  
Vol 75 (10) ◽  
pp. 3146-3152 ◽  
Author(s):  
Niamh Toomey ◽  
�ine Monaghan ◽  
S�amus Fanning ◽  
Declan Bolton
Keyword(s):  
Antibiotic Resistance ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Marker Genes ◽  
Natural Environments ◽  
In Vivo Models ◽  
Filter Mating ◽  
Mating Method

ABSTRACT Three wild-type dairy isolates of lactic acid bacteria (LAB) and one Lactococcus lactis control strain were analyzed for their ability to transfer antibiotic resistance determinants (plasmid or transposon located) to two LAB recipients using both in vitro methods and in vivo models. In vitro transfer experiments were carried out with the donors and recipients using the filter mating method. In vivo mating examined transfer in two natural environments, a rumen model and an alfalfa sprout model. All transconjugants were confirmed by Etest, PCR, pulsed-field gel electrophoresis, and Southern blotting. The in vitro filter mating method demonstrated high transfer frequencies between all LAB pairs, ranging from 1.8 � 10−5 to 2.2 � 10−2 transconjugants per recipient. Transconjugants were detected in the rumen model for all mating pairs tested; however, the frequencies of transfer were low and inconsistent over 48 h (ranging from 1.0 � 10−9 to 8.0 � 10−6 transconjugants per recipient). The plant model provided an environment that appeared to promote comparatively higher transfer frequencies between all LAB pairs tested over the 9-day period (transfer frequencies ranged from 4.7 � 10−4 to 3.9 � 10−1 transconjugants per recipient). In our test models, dairy cultures of LAB can act as a source of mobile genetic elements encoding antibiotic resistance that can spread to other LAB. This observation could have food safety and public health implications.

scholarly journals In vivo assessment and characterization of lactic acid bacteria with probiotic profile isolated from human milk powder

2020 ◽  
Author(s):  
Blanca Rosa Aguilar Uscanga ◽  
Ariana Rodríguez Arreola ◽  
Josué R. Solís Pacheco ◽  
Monique Lacroix ◽  
Edgar Balcazar López ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Human Milk ◽  
Milk Powder ◽  
In Vivo Assessment

scholarly journals Screening and Characterization of Lactic Acid Bacteria with Broad-Spectrum Antimicrobial Activity from Tibetan Qula, a Yak Milk Cheese

2021 ◽  
Author(s):  
Bei Zhang ◽  
Jun Chen ◽  
Guofang Wu ◽  
Lei Wang ◽  
Guangyong Qin ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Lactic Acid ◽  
Tibetan Plateau ◽  
Lactic Acid Bacteria ◽  
Lactobacillus Plantarum ◽  
Broad Spectrum ◽  
Pathogenic Bacteria ◽  
The Tibetan Plateau ◽  
N Source ◽  
Rdna Sequencing

Abstract BackgroundLactic acid bacteria with natural, effective antibacterial activity, safe and reliable characteristic, gradually become one of the key technologies in food fermentation applications, food preservation and other fields. In this study, 112 presumptive lactic acid bacteria isolated from Tibetan Qula, a fermented yak cheese popular in the Tibetan plateau, were screened for potential probiotic microorganism with antimicrobial activity.Results12 lactic acid bacteria were found to have antibacterial activity, and strain QZ50 in particular showed broad-spectrum inhibition against pathogenic bacteria, which retained its antibacterial activity after sequential removal of acids and hydrogen peroxide, indicating the production of a broad-spectrum bacteriocin that could inhibite Micrococcus luteus ATCC 28001, Staphylococcus aureus ATCC 26003, Bacillus subtilis ATCC 63501, Escherichia coli ATCC 30105, Pseudomonas aeruginosa ATCC 10104, and Salmonella enterica ATCC 50094. Strain QZ50 was identified as Lactobacillus plantarum based on physicochemical characteristics and 16S rDNA sequencing. And the optimum production conditions were evaluated to obtain the highest yield of plantaricin QZ50. The optimum medium, temperature, initial pH, and inoculum amount for plantaricin QZ50 production were Man, Rogosa, and Sharpe (MRS), 30°C, 6.5, and 3%, respectively. In addition, different C source, N source and stimulating factors in medium show significant effects on plantaricin QZ50 production (P ＜ 0.05). The optimum C and N source were respectively glucose and yeast extract, and 2% Tween 80 contributed highest production of plantaricin QZ50. Plantaricin QZ50 exhibited strong heat stability and remained activity at pH 2.0–8.0. In addition, plantaricin QZ50 was inactivated by pepsin, proteinase K, trypsin, papain, and chymotrypsin.ConclusionsSome strains of Lactobacillus isolated from the Qula in the Tibetan plateau have good antibacterial activity which could be considered as potential probiotic. The strain of Lactobacillus plantarum QZ50, with a broad-spectrum, stable, safe, and natural antibiotic, has potential applications as a food biopreservative.

scholarly journals Characterization of a bacteriocin as biopreservative synthesized by indigenous lactic acid bacteria from dadih soya traditional product used in West Sumatra, Indonesia

2020 ◽  
Vol 21 (9) ◽  
Author(s):  
Endah Retnaningrum ◽  
TANIA YOSSI ◽  
RINI NUR’AZIZAH ◽  
FADILLA SAPALINA ◽  
PERISKILA DINA KALI KULLA
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Proteolytic Enzymes ◽  
Antibacterial Activities ◽  
Sequencing Analysis ◽  
Rdna Sequencing ◽  
Nacl Concentration ◽  
West Sumatra ◽  
Antibacterial Spectrum

Abstract. Retnaningrum E, Yossi T, Nur'azizah R, Sapalina F, Kulla PDK. 2020. Characterization of a bacteriocin as biopreservative synthesized by indigenous lactic acid bacteria from dadih soya traditional product used in West Sumatra, Indonesia. Biodiversitas 21: 4192-4198. A total of 4 isolates of lactic acid bacteria (strain BDL08, BDL11, BDL12, and BDL13) isolated from dadih soya were investigated for the ability to produce bacteriocin based on their antibacterial activities against Listeria monocytogenes ATCC 7644. Based on comparative 16S rDNA sequencing analysis, isolates BDL 11, BDL12, and BDL13 were identified as Lactobacillus plantarum while the isolates BDL 08 was identified as Leuconostoc mesenteroides. All crude bacteriocins producing strains revealed broad antibacterial spectrum against 7 different indicator bacteria, including Gram-positive and Gram-negative bacteria. L. plantarum BDL11 displayed the highest bacteriocin activity relative to others. The bacteriocin produced by the strain was not affected by pH, heating, and NaCl concentration but was sensitive to proteolytic enzymes. This research indicated that the bacteriocin in the food industry has the potential to be used as a biopreservative.

Morphological and Molecular Characterization of Lactic acid Bacteria from intestine of Freshwater Fishes and Their Possible Role in Probiotic Feeding in Aquaculture

2015 ◽  
Vol 5 (3) ◽  
pp. 101-106
Author(s):  
Dinesh Kumar Kumawat ◽  
Nadim Chishty
Keyword(s):  
Antibacterial Activity ◽  
Lactic Acid ◽  
Salt Tolerance ◽  
Lactic Acid Bacteria ◽  
Bile Salt ◽  
Micrococcus Luteus ◽  
Food Preservation ◽  
Rrna Gene ◽  
Gram Staining

  Lactic acid bacteria (LAB) are a group of gram positive, non spore forming, cocci or rod shaped, catalase negative organisms. Generally they are known as "GRAS" (Generally Recognized As Safe) organisms. In the present study, an attempt was made to isolate lactic acid bacteria from gut of two freshwa-ter fishes in Udaipur region to obtain various efficient LAB strains for indus-trial applications. A total of 12 bacterial isolates were isolated from different fishes caught from Pichola lake, Udaipur on MRS agar at 37oC. All the isolates were tested for catalase activity, colony morphology and gram staining. Further these isolates were checked for technological properties (antibacterial activity, antibiotic susceptibility and bile salt tolerance). Based on technologi-cal properties results, two isolate (F-4 & F-11) have been tentatively selected for use as probiotic cultures in the aquaculture industries. They have strong antibacterial activity against various pathogens viz. Staphylococcus aureus (ATCC 9144), Micrococcus luteus (ATCC 10240), Listeria monocytogens (MTCC 1143) and have bile salt tolerance limit up to 0.7%. These are the main features for selection of probiotic candidates. To differentiate the same species molecular typing was attempted, 16S rRNA gene amplification was done using specific 16S rDNA primers and amplified gene products were di-gested with different restriction endonuclease enzymes viz EcoRI and HindIII. A total of 2 different groups out of 12 isolates were obtained. This finding reveals that isolated LAB are useful for aquaculture and also used in food preservation.

scholarly journals Purification and Characterization of a Novel Bacteriocin Pediocin Z-1 Produced by Pediococcus Pentosaceus Z-1 Screened From Jinhua Ham

2020 ◽  
Author(s):  
Xiaohong Yu ◽  
Zhicai Yang ◽  
Keyue Li ◽  
Rui Liu ◽  
Huijie Pei ◽  
...  
Keyword(s):  
Thermal Stability ◽  
Antibacterial Activity ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Food Industry ◽  
Acid Base ◽  
Pediococcus Pentosaceus ◽  
Base Stability ◽  
Jinhua Ham

Abstract Background: Lactic acid bacteria (LAB) can produce bacteriostatic substances, among which bacteriocins attracted wide attention in food preservation for decades. Up to date, nisin (class I bacteriocins) has been considered to be the only bacteriocin produced by Lactococcus lactis strains for commercial use. Moreover, there are many other reports concerning the isolated bacteriocins for potential application in food industry while some exhibited a narrow bactericidal spectrum, thermal stability and low acid-base stability. Jinhua ham is a representative of traditional dry-cured meat product in China. The microbial community structure and diversity was speculated to be responsible for flavor and quality formation of Jinhua ham, protecting from the spoilage microorganisms contamination. However, no studies was performed to investigate the bacteriocin from LAB in Jinhua ham. Thus, the objective of this study was to screen out high-efficiency, safe and non-toxic bacteriocin-producing lactic acid bacteria (LAB) from Jinhua Ham and subsequently perform the purification, identification and characterization of its bacteriocin. Results: The bacteriocin-producing LAB was screened from Jinhua ham and then designated as Pediococcus pentosaceus Z-1 by colony morphology and 16S rDNA sequencing. The bacteriocin was then crudely extracted from the bacterial cell-free supernatant by pH adsorption, and further purified by cellulose DEAE-52 ion exchange and Sephadex G-50 chromatography columns. The tricine-SDS-PAGE electrophoresis showed a highly purified protein band with 8227.35 Da with 60 amino acids identified by MALDI-TOF-MS analysis. The bacteriocin was named as pediocin Z-1 and its antibacterial activity exhibited an acid-base stability between pH 2-10 and a thermal stability at a range of 50-110°C. The pediocin Z-1 was sensitive to proteases and showed an inhibitory effect against Gram-positive bacteria and Gram-negative bacteria, including Listeria monocytogenes, Staphylococcus aureus, Salmonella typhimurium, Salmonella potsdam and Escherichia coli. Conclusions: Our data suggest that pediocin Z-1 has a comparable effect of antibacterial activity with nisin and it is promising that pediocin Z-1 has great potential for application prospect in the food industry.

scholarly journals Rep PCR Characterization of Lactic Acid Bacteria Isolated From Raw Cows’ Milk in Algeria.

2021 ◽  
Author(s):  
Akil LOMANI ◽  
Dennis S Nielsen ◽  
Larbi Ahmed Amine ◽  
Tahri Ahmed ◽  
Mediani Ahmed
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Bile Salts ◽  
Acid Tolerance ◽  
16S Rrna Gene Sequencing ◽  
Fermented Milk ◽  
Haemolytic Activity ◽  
Rrna Gene ◽  
Rrna Gene Sequencing

Abstract Milk and indigenous fermented, milk based products are a rich source of lactic acid bacteria (LAB) and may serve as a source of potential probiotics. In the present study LAB were isolated from raw cows’ milk sampled at five different Algerian farms. A total of 24 Gram positive, catalase negative isolates where identified to species level using a combination of (GTG) 5-based rep-PCR fingerprinting and 16S rRNA gene sequencing. All isolates were identified as Enterococcus faecium and all isolates had highly similar rep-PCR profiles. Four representative isolates were screened for acid tolerance, bile salts tolerance, antimicrobial susceptibility, antibacterial activity and haemolysis. The four selected strains all exhibited good tolerance to low pH (2, 3, and 4), and to bile salts (concentrations of 0.5%, 1%, and 2%) and were sensitive to chloramphenicol, vancomycin, tetracycline, gentamicine and peniciline G, but were resistant to oxaciline. Cell-free supernatants of the four tested strains all inhibited Staphylococcus aureus, Escherichia coli and Listeria monocytogenes but not Salmonella Typhi. No haemolytic activity was observed.

Isolation of Lactic acid bacteria showing antagonistic activity against Streptococcus mutans

MedPharmRes ◽  
2020 ◽  
Vol 4 (2) ◽  
pp. 23-33
Author(s):  
Thi Hong-Hanh Nguyen ◽  
Tuan-Anh Le
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Streptococcus Mutans ◽  
Colorimetric Assay ◽  
Acid Tolerance ◽  
Strong Acid ◽  
Inhibitory Effect ◽  
Human Saliva ◽  
Healthy Human ◽  
Rdna Sequencing

Background and Objectives: Streptococcus mutans is a major pathogen that initiates dental plaque and dental caries due to its strong acid production and biofilm-forming ability. Recent studies showed that lactic acid bacteria (LAB), exert useful properties which make them become potential tools to fight against S. mutans and its relating diseases. The current study aimed to isolate and screen for LAB strains that could inhibit S. mutans growth as well as its biofilm production. Methods and Results: Using the spread plate technique, fifty-one LAB strains were isolated from fermented vegetables and healthy human saliva. From that, fourteen isolates showed the inhibitory effect on S. mutans growth in well-diffusion assay. These strains were then screened on the ability in reducing S. mutans biofilm formation by colorimetric assay. Next, potential LAB was evaluated on some probiotic characteristics including saliva amylase tolerance, acid tolerance, bile tolerance as well as antibiotic susceptibility properties. As a result, four strains showed the most promising features were further tested for the ability to reduce Streptococcus mutans biofilm by scanning electron microscopy (SEM) observation and by expression analysis of biofilm-forming genes (gtfB and luxS) using RT-qPCR method. Finally, these four strains were identified to species level using API® 50 CHL kit and 16S rDNA sequencing method. The result showed that two strains belong to L. plantarums, one belongs to L. brevis and the other is Weisella confusa. Conclusion: This study was successful in isolating LAB that showed promising probiotic effects against S. mutans.

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