Accelerated Ageing Mediated Seed Longevity Prediction and Assessment of Seed Deterioration Pattern through 2D-Gel Electrophoresis in Chickpea (Cicer arietinum L.)

2021 ◽  
Author(s):  
Shivasharanappa S. Patil ◽  
Ashok S. Sajjan ◽  
N.K. Biradarpatil ◽  
P.U. Krishnaraj ◽  
I.S. Katageri
Keyword(s):  
Seed Storage ◽  
Quality Parameters ◽  
Seed Longevity ◽  
Accelerated Ageing ◽  
Natural Ageing ◽  
Ambient Conditions ◽  
Seed Deterioration ◽  
Germination Potential ◽  
Deterioration Process ◽  
Ageing Methods

Background: Accelerated ageing is a method to assess seed storage performance by exposing the seeds to higher temperature and relative humidity to make seeds lose its vigour and viability quickly. The seed physiological parameters are then compared with the natural ageing to derive certain conclusions. But, the molecular pattern of seed deterioration under accelerated ageing varies compared to that of natural ageing.Methods: Chickpea, seed lots of variety JG-11 and Annigeri-1 were subjected to natural ageing in cloth bag with nine per cent initial seed moisture under ambient conditions. Simultaneously, representative sample of same seed lot were also aged at 41oC and 95±2% humidity up to 120 hours.Result: Both the ageing methods had significant negative effect on seed physiological and biochemical quality parameters. The germination potential of seeds tested after 48 hours of accelerated ageing (83.5%) was equal 10 months of natural ageing (85%) in JG-11, while also in Annigeri-1, 48 hours of accelerated ageing (78%) was nearly equal to 10 months of natural ageing (80%). The protein expression analyzed through 2D-PAGE at similar germination potential brought out by two independent ageing methods showed higher protein down regulation ratio (3.4) in accelerated ageing than in natural ageing (3.2) unveiling its rapidity in the seed deterioration process. Therefore, accelerated ageing can be used for predicting chickpea seed longevity.

Prediction of storability in soybean seeds through accelerated ageing technique [Glycine max (L.) Merill]

2017 ◽  
Author(s):  
Shivasharanappa . ◽  
S. Patil ◽  
S. R. Doddagoudar ◽  
Vijay Kumar Kurnalliker ◽  
Rakesh . ◽  
...  
Keyword(s):  
Seed Quality ◽  
Germination Percentage ◽  
Quality Parameters ◽  
Accelerated Ageing ◽  
Soybean Seeds ◽  
Natural Ageing ◽  
Ambient Conditions ◽  
Seedling Vigour ◽  
Subsequent Increase ◽  
Vigour Index

An attempt was made to predict the relative storability of seeds of soybean varieties (JS-335 and DSB-21) through accelerated ageing technique in comparison with that of natural ageing where both the varieties had similar initial germination (93 %). The seeds were subjected to accelerated ageing conditions at 41 oC temperature and 95 ± 5 per cent relative humidity up to 180 hours. While, for natural ageing, the seeds were packed in cloth bag with the initial moisture of 9.5 per cent and stored under ambient conditions. The experimental results revealed that with the subsequent increase in accelerated ageing duration from 24 hours onwards up to 180 hours, the seed quality parameters viz., seed germination percentage, mean germination time, seedling vigour index and dehydrogenase enzyme activity decreased significantly. While the electrical conductivity increased significantly. When the accelerated ageing results were compared with that of natural ageing, accelerated ageing for 132 hours (67 % germination) was nearly equal to nine months of natural ageing (69 % germination) in case of JS-335 whereas in DSB-21, 144 hours of accelerated ageing (70 % germination) was equal to nine months of natural ageing (70 % germination). Hence, the accelerated ageing test can be used to predict the relative storability of soybean seeds. Hence, the seed producers can take decision regarding which seed lot can be stored for longer time and which one should be immediately sold to the market without any compromise in seed quality. It also helps in taking other management decisions.

scholarly journals Prediction of storability of organically produced paddy seeds through natural and accelerated ageing techniques

2016 ◽  
Vol 8 (3) ◽  
pp. 1638-1642
Author(s):  
Gajendra Khidrapure ◽  
S. N. Vasudevan ◽  
S. R. Doddagoudar ◽  
A. G. Sreenivas ◽  
Satyanarayana Rao
Keyword(s):  
Seed Germination ◽  
Seed Quality ◽  
Foliar Spray ◽  
Storage Period ◽  
Quality Parameters ◽  
Accelerated Ageing ◽  
Natural Ageing ◽  
Ambient Conditions ◽  
Seedling Length ◽  
Cent Relative Humidity

The present study was conducted to know the storage potential of organically produced paddy seeds in the Department of Seed Science and Technology, University of Agricultural Sciences, Raichur. The seed lot were divided into two parts, one part was stored in cloth bag for a period of 12 months under ambient conditions. At thesame time another set of seeds were subjected to accelerated ageing at 42 + 10C temperature and 90 per cent relative humidity (RH) for a period of 0-12 days. Among the ageing methods, artificially aged seeds showed drastic decreases in seed quality as compared to natural ageing. Among the treatments T9 (37.5 % FYM + 37.5 % vermicompost + 25 % neem cake + foliar spray of panchagavya on 30, 60, 90 and 120 DAT) recorded significantly highest seed quality parameters viz., seed germination (97.81 %), seedling length (29.42 cm) and SVI (2878) at initial stage in both the method of aging and at the end of storage period; seed germination (71.23 and 87.33 %), seedling length (19.66 and 27.00 cm) and SVI (1400 and 2358) in accelerated ageing (AA) and natural ageing (NA) respectively, whereas, lowest in control (Inorganic treatment). The seed quality parameters of four days of AA were similar to that of six months of NA. Hence, storability of organically produced paddy seeds were better as compared to inorganic seeds and it can be predicted that four days of AA is equal to six months of NA. The information generated will be useful in retention or disposal of a particular variety or seed lot.

scholarly journals Histochemistry of backcross 1 of oil palm seeds at different storage periods

2020 ◽  
Vol 5 (1) ◽  
pp. 52
Author(s):  
Ernayunita Ernayunita ◽  
Taryono Taryono ◽  
Prapto Yudono
Keyword(s):  
Oil Palm ◽  
Oil Quality ◽  
Seed Storage ◽  
Fat Content ◽  
Endosperm Tissue ◽  
Seed Deterioration ◽  
Seed Content ◽  
Deterioration Process ◽  
Histochemical Tests ◽  
Food Reserves

Oil palm backcross 1 is the result of the crossing between E. oleifera and E. guineensis, followed by backcrossing to one of its parents. It has several advantages, including slow height growth and good oil quality, thereby having the potential to be developed. However, it also has a short seed shelf life, which might be inherited from E. oleifera that has relatively quick seed deterioration. This is problem to the breeding program, and there have not been many studies on the seed deterioration process. A histological examination can determine the composition of food reserves in seed endosperm tissue. Therefore, it is necessary to assess the histochemistry of seeds concerning the process of seed deterioration. Histochemical tests with Sudan III, Milon, and IKI reagents were used as histochemical tests of fat, protein and carbohydrate seed content with different seed storage periods, i.e. 4, 3, 2, and less than 1 year. The result showed that the fat content decreased during the storage periods. Based on carbohydrates and proteins staining, there were only very few substances , which were difficult to distinguish among the storage periods, so that this analysis could not be used as the determining indicator of seed deterioration. The fat content was a determining factor of seed deterioration and quality. The oil palm seed storage up to 3-4 years led to the reduction in the fat content in significant quantities compared to the fresh seeds stored less than 1 year as they still contained a lot of fat in the cell.

scholarly journals Impact of harvest stage on seed yield quality and storability of french bean

2016 ◽  
Vol 40 (4) ◽  
pp. 641-656
Author(s):  
Md Rayhan Shaheb ◽  
Md Nazmul Islam ◽  
Ashratun Nessa ◽  
Md Altab Hossain ◽  
Ayesha Sarker
Keyword(s):  
Seed Yield ◽  
Seed Quality ◽  
Seed Storage ◽  
Quality Parameters ◽  
French Bean ◽  
Yield Potential ◽  
Ambient Conditions ◽  
Yellow Colour ◽  
Brown Colour ◽  
The Impact

Good quality seeds are one of the least expensive but vital factors influencing yield potential and key to agriculture progress. Studies were conducted both in the field and laboratory with the objective to observe the impact of harvest stage on the seed, quality and storability of French bean. Five harvest stages viz. H1- deep green with light yellow colours of pod, H2-50% green and 50% yellowing of pods, H3-light brown with few yellow colour pods, H4-90% brown colour of pods and H5-100% brown colour and dried pods were considered as treatments for field trial. Harvested seeds were then stored in both cool room and ambient conditions up to 16 months and performed seed quality studies in every 4 months. The treatments combination of laboratory studies were T1: H1 seed storage in cool room (SSCR), T2: H1 seed storage in ambient (SSAB), T3: H2 SSCR, T4: H2 SSAB; T5: H3 SSCR; T6: H3 SSAB; T7: H4 SSCR; T8: H4 SSAB; T9: H5 SSCR and T10: H5 SSAB. Experiments were laid out in a RCBD and CRD in the field and laboratory, respectively. Results revealed that the highest seed yield and quality of French bean was observed in H3. On the contrary, seed harvested in H4 and stored in cool room (with the mean temperature 18-20oC and relative humidity around 60-70%) recorded the highest storability compared to ambient condition. However, seeds harvested in H3 and H5 were also showed better storability in cool room as well as ambient conditions. To sum up, all the seed quality parameters were satisfactorily well up to 12 months of storage then it declined in quality.Bangladesh J. Agril. Res. 40(4): 641-656, December 2015

scholarly journals Assessment of Natural Ageing Related Physio-Biochemical Changes in Onion Seed

Agriculture ◽  
2019 ◽  
Vol 9 (8) ◽  
pp. 163 ◽  
Author(s):  
Brar ◽  
Kaushik ◽  
Dudi
Keyword(s):  
Seedling Establishment ◽  
Seed Quality ◽  
Seed Viability ◽  
Seed Storage ◽  
Germination Percentage ◽  
Quality Parameters ◽  
Biochemical Changes ◽  
Ambient Conditions ◽  
Onion Seed ◽  
One Year

This research was performed for a period of three years to determine the effect of natural seed ageing on different physiological and biochemical changes in three extensively cultivated onion cultivars in Northern India. Seed storage studies showed that germination percent, seed viability and other seed quality parameters decreased significantly with increasing storage time. The onion seed can be stored for a maximum period of one year under ambient conditions to maintain required germination percentage (>70%). Overall, Hisar Onion-4 stored better when compared to Hisar Onion-3 and Hisar-2. As the ageing progressed the seed antioxidants, namely superoxide dismutase (SOD), catalase (CAT), dehydrogenase (DHA) and peroxidase (POD) decreased significantly (p < 0.05) in all cultivars whereas, the electrical conductivity (EC) of seed leachates increased significantly. Under the field conditions, seedling establishment percent (SE) onion seed was positively correlated (R2 = 0.98; p < 0.05) with germination percent (GP). Overall, more than one year period of seed storage was associated with poor germination and seedling establishment potential in onion.

Seed Longevity and Its Association with Agronomic Traits in Soybean [Glycine max (L.) Merrill]

2021 ◽  
Author(s):  
Shreya Singh ◽  
Rajesh Kanwar ◽  
R.K. Kapila ◽  
K.C. Dhiman
Keyword(s):  
Agronomic Traits ◽  
Strong Association ◽  
Selection Criterion ◽  
Germination Percentage ◽  
Indirect Selection ◽  
Seed Longevity ◽  
Oilseed Crop ◽  
Ambient Conditions ◽  
Time Period ◽  
Poor Seed

Background: Soybean is an important oilseed crop but it has poor seed longevity. Studies on seed longevity are very complex and hence, an indirect selection criterion should be identified. In this study, seed longevity and its association with agronomic traits of seven soybean varieties was evaluated in the year 2019-2020.Methods: Seed were kept for storage under ambient conditions for a period of 16 months (January 2019 to May 2020). Their germination was recorded at bimonthly intervals and seed longevity was inferred as the time period in months up to which the seed maintains its germination percentage above IMSCS.Result: Accordingly, Palam Early Soya 1 and Him Palam Hara Soya were classified as having short seed longevity (12 months). Hara Soya, Shivalik, Him Soya and Palam Soya were classified as having medium seed longevity (14 months). Whereas, PS 1556 was classified as having long seed longevity ( greater than 16 months). Further, it was found that seed longevity was positively associated with number of pods per plant and seed yield, and negatively associated with number of branches per plant and 100-seed weight. This study presents an indirect selection criterion based on some easily accessible parameters which have strong association with seed longevity.

scholarly journals Alterations in the lipid and soluble sugar content of melanoxylon brauna seeds during natural and accelerated ageing

2010 ◽  
Vol 32 (3) ◽  
pp. 152-162 ◽  
Author(s):  
Viviana Borges Corte ◽  
Eduardo Euclydes de Lima e Borges ◽  
José Francisco de Carvalho Gonçalves ◽  
Mirian Sousa Silva
Keyword(s):  
Fatty Acids ◽  
Tropical Forest ◽  
Sugar Content ◽  
Soluble Sugar ◽  
Soluble Sugars ◽  
Forest Tree ◽  
Accelerated Ageing ◽  
Natural Ageing ◽  
Metabolic Reactions ◽  
Tree Seeds

The decay of seeds is irreversible and at best can only be delayed by applying techniques that reduce the velocity of the metabolic reactions involved. There is little information on the biochemistry of tropical forest tree seeds related to their storability. It was investigated the influence of the composition of lipids and soluble sugars of two storage compartments, the cotyledons and the embryonic axis, of Melanoxylon brauna Schot. (Leguminosae- Caesalpinioideae), a hardwood known as black brauna, seeds stored at 20 ºC for 0, 3, 6, 9 and 12 months (natural ageing) and for 0, 24, 48, 72 and 96 hours at 40 ºC (accelerated ageing). The levels of fatty acids and monosaccharides varied differentially in each of the embryo storage compartments. Changes in oligosaccharide levels were similar for both types of ageing, diminishing in both compartments. Ageing can be attributed to the significant decrease of oligosaccharides and the increase of glucose in both types of ageing and both embryo compartments.

scholarly journals Optimization and sensitivity analysis of fast ethanol assay in maize seeds

2019 ◽  
Vol 41 (1) ◽  
pp. 97-107 ◽  
Author(s):  
Damrongvudhi Onwimol ◽  
Thunyapuk Rongsangchaicharean ◽  
Pitipong Thobunluepop ◽  
Tanapon Chaisan ◽  
Wanchai Chanprasert
Keyword(s):  
Quality Assessment ◽  
Ethanol Production ◽  
Seed Quality ◽  
Storage Conditions ◽  
Ambient Conditions ◽  
Maize Seed ◽  
Seed Deterioration ◽  
Maize Seeds ◽  
Hermetic Seal

Abstract: The evaluation of seed deterioration is very important to control the quality of the seeds stored. This study aimed to investigate the potential of fast ethanol assay for seed quality assessment of maize stored under different conditions. The first experiment was to determine the incubating temperature, incubating time, and amount of seed used in the assay. The results showed that the best protocol for the detection of headspace ethanol was incubation of 3 g of maize seed with 20% moisture content (wet basis) in a 20 mL gas chromatography vial at 70 °C for 1.5 h. The assay induced approximately 200-700 µg.L-1 of headspace ethanol, which was sufficient to identify seeds with different vigour levels. In the second experiment, the optimal conditions were used for quality assessment in aged maize seed stored for 12 months under different storage conditions. The increase in the ethanol production of stored maize seed under the controlled conditions (15 °C and 20% RH in the hermetic seal) was lower than under ambient conditions. The ethanol production levels of maize seed samples at the start of storage was significantly lesser than at six months storage (p < 0.05). The test limitations in deteriorated seed with different cultivars and ages will be discussed.

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