Unactivated platelet leukocyte autoplasma in the treatment of non scarring alopecia

2016 ◽  
Vol 19 (6) ◽  
pp. 369-377
Author(s):  
G. A Ragimov ◽  
O. Yu Olisova ◽  
Kseniya G. Egorova
Keyword(s):  
Growth Factors ◽  
Clinical Cure ◽  
Clinical Observation ◽  
Platelet Rich Plasma ◽  
White Blood Cells ◽  
Treatment Method ◽  
Hair Follicles ◽  
Scarring Alopecia ◽  
Platelet Concentration

The literature review of the cellular functioning mechanisms of the hair follicles, the role of stem cells in the life cycle of the hair, the major effects of growth factors are presented. Authors, patented treatment method of non scarring alopecia is described. The technology ofpreparation and method of use of unactivated platelet leukocyte autoplasma in various forms of alopecia are described. Own clinical observation of 60 patients with non scarring alopecia and results of their unactivated platelet leukocyte autoplasma are presented. 80% of patients had a clinical cure. Investigations of platelet concentration, white blood cells and growth factors in platelet-rich plasma and platelet leukocyte unactivated autoplasma were performed. The results are shown in the article.

scholarly journals Role of White Blood Cells in Blood- and Bone Marrow-Based Autologous Therapies

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
William King ◽  
Krista Toler ◽  
Jennifer Woodell-May
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Growth Factors ◽  
Blood Cells ◽  
Platelet Rich Plasma ◽  
White Blood Cells ◽  
Complex Mixture ◽  
Negative Results

There has been significant debate over the role of white blood cells (WBCs) in autologous therapies, with several groups suggesting that WBCs are purely inflammatory. Misconceptions in the practice of biologic orthopedics result in the simplified principle that platelets deliver growth factors, WBCs cause inflammation, and the singular value of bone marrow is the stem cells. The aim of this review is to address these common misconceptions which will enable better development of future orthopedic medical devices. WBC behavior is adaptive in nature and, depending on their environment, WBCs can hinder or induce healing. Successful tissue repair occurs when platelets arrive at a wound site, degranulate, and release growth factors and cytokines which, in turn, recruit WBCs to the damaged tissue. Therefore, a key role of even pure platelet-rich plasma is to recruit WBCs to a wound. Bone marrow contains a complex mixture of vascular cells, white blood cells present at much greater concentrations than in blood, and a small number of progenitor cells and stem cells. The negative results observed for WBC-containing autologous therapies in vitro have not translated to human clinical studies. With an enhanced understanding of the complex WBC biology, the next generation of biologics will be more specific, likely resulting in improved effectiveness.

scholarly journals Fetal hyperinsulinemia increases farnesylation of p21 Ras in fetal tissues

2001 ◽  
Vol 281 (2) ◽  
pp. E217-E223 ◽  
Author(s):  
Elizabeth Stephens ◽  
Patti J. Thureen ◽  
Marc L. Goalstone ◽  
Marianne S. Anderson ◽  
J. Wayne Leitner ◽  
...  
Keyword(s):  
Growth Factors ◽  
Molecular Mechanisms ◽  
Fetal Liver ◽  
White Blood Cells ◽  
Causative Factor ◽  
Ras Proteins ◽  
Direct Infusion ◽  
Fetal Tissues ◽  
P21 Ras

Even though the role of fetal hyperinsulinemia in the pathogenesis of fetal macrosomia in patients with overt diabetes and gestational diabetes mellitus seems plausible, the molecular mechanisms of action of hyperinsulinemia remain largely enigmatic. Recent indications that hyperinsulinemia “primes” various tissues to the mitogenic influence of growth factors by increasing the pool of prenylated Ras proteins prompted us to investigate the effect of fetal hyperinsulinemia on the activitiy of farnesyltransferase (FTase) and the amounts of farnesylated p21 Ras in fetal tissues in the ovine experimental model. Induction of fetal hyperinsulinemia by direct infusion of insulin into the fetus and by either fetal or maternal infusions of glucose resulted in significant increases in the activity of FTase and the amounts of farnesylated p21 Ras in fetal liver, skeletal muscle, fat, and white blood cells. An additional infusion of somatostatin into hyperglycemic fetuses blocked fetal hyperinsulinemia and completely prevented these increases, specifying insulin as the causative factor. We conclude that the ability of fetal hyperinsulinemia to increase the size of the pool of farnesylated p21 Ras may prime fetal tissues to the action of other growth factors and thereby constitute one mechanism by which fetal hyperinsulinemia could induce macrosomia in diabetic pregnancies.

Similarities between platelet contraction and cellular motility during mitosis: role of platelet microtubules in clot retraction

1976 ◽  
Vol 20 (3) ◽  
pp. 569-588
Author(s):  
F.C. Chao ◽  
D. Shepro ◽  
J.L. Tullis ◽  
F.A. Belamarich ◽  
W.A. Curby
Keyword(s):  
Protein Synthesis ◽  
Energy Metabolism ◽  
Platelet Rich Plasma ◽  
Cellular Motility ◽  
Optimal Conditions ◽  
Clot Retraction ◽  
Platelet Concentration ◽  
High Concentrations ◽  
Initial Delay

The effects of inhibitors of mitosis, energy metabolism and protein synthesis on clot retraction were investigated. The results show that (1) Incubation of colchicine (0-01-0-1 mM) with platelet-rich plasma (PRP) inhibits the subsequent retraction of clots derived from diluted PRP. (2) Inhibition of clot retraction by high concentrations of colchicine (up to 40 mM) can be overcome by increasing the platelet concentration in the system. (3) Incubation of clots in colchicine or 80% D2O solutions inhibits their retraction. Exposure of partially retracted clots to these agents is without effect. (4) Hydrostatic pressure retards clot retraction. (5) Incubation of PRP with either 2-deoxy-D-glucose or antimycin alone does not affect clot retraction, but a combination of these agents is inhibitory. (6) Clot retraction is not inhibited by puromycin or cycloheximide. (7) Platelets in retracting clots have constricted regions containing microfilaments and pseudopods containing microtubules. Fibrin strands are progressively condensed around the constricted regions as retraction advances. (8) The development of platelet constriction, platelet pseudopods and the intracellular microfilaments are delayed in colchicinized clots, corresponding to the retardation of retraction. Following the initial delay of retraction colchicinized clots, like controls, show condensation of fibrin strands adjacent to these constricted areas of platelets containing microfilaments. The formation of pseudopods is impaired and no microtubules are found in platelets in the presence of colchicine. The above results suggest that the thrombin-induced platelet contraction during clot retraction is a coordinated movement, which, under optimal conditions involves both microtubules and microfilaments. The contraction of microfilaments produces the constriction of platelets and brings about clot retraction by reducing the angle between fibrin strands. Platelet microtubules are related to the development of pseudopods and play a supplementary role in facilitating microfilament-mediated cellular constriction. The similarities between platelet contraction and cellular motility in mitosis is discussed.

The effect of IGF-I on the growth of secondary hair follicles of the Cashmere goat in vitro

1997 ◽  
Vol 1997 ◽  
pp. 170-170
Author(s):  
H. Galbraith ◽  
D. Sims ◽  
D. Hazlerigg
Keyword(s):  
Growth Factors ◽  
Hair Follicle ◽  
Human Hair ◽  
Hair Follicles ◽  
Cashmere Goat ◽  
Igf I ◽  
Hair Follicle Cycle ◽  
Insulin Like Growth Factors

Factors regulating the growth of Cashmere fibre and the hair follicle cycle are poorly understood. Insulin-like growth factors (IGFs) or insulin at higher concentrations, have been shown to stimulate in vitro growth of human hair follicles (Philpott et al, 1994). The role of such mitogens in the production of cashmere fibre by the Cashmere goat has not been previously investigated. The objective the study reported here was to investigate the growth of hair follicles in the absence and presence of insulin or IGF-I using our established in vitro technique.

Biologic Enhancement of Cartilage Repair: The Role of Platelet-Rich Plasma and Other Commercially Available Growth Factors

2015 ◽  
Vol 31 (4) ◽  
pp. 777-783 ◽  
Author(s):  
Ramon Cugat ◽  
Xavier Cuscó ◽  
Roberto Seijas ◽  
Pedro Álvarez ◽  
Gilbert Steinbacher ◽  
...  
Keyword(s):  
Growth Factors ◽  
Cartilage Repair ◽  
Platelet Rich Plasma

Review of concentration yields in commercially available platelet-rich plasma (PRP) systems: a call for PRP standardization

2019 ◽  
Vol 44 (6) ◽  
pp. 652-659 ◽  
Author(s):  
Priyal P Fadadu ◽  
Anthony J Mazzola ◽  
Corey W Hunter ◽  
Timothy T Davis
Keyword(s):  
Systematic Review ◽  
Growth Factors ◽  
Platelet Rich Plasma ◽  
White Cell Count ◽  
Pain Medicine ◽  
Inclusion Criteria ◽  
Separation Systems ◽  
Platelet Concentration ◽  
Large Heterogeneity ◽  
Promising Treatment

Platelet-rich plasma (PRP) has become increasingly popular in pain medicine with hopes of becoming a safe, effective alternative to routine treatments. However, given its autologous nature, PRP injectate may differ depending on the specific manufacturer and protocol. Currently, there is no standardization of reporting protocol. This systematic review compiles and standardizes values on PRP preparation and final product composition of platelets, white cell count, and growth factors for ease of comparison. On review of 876 studies, 13 studies were selected according to our inclusion criteria. Data from 33 PRP systems and protocols were extracted and standardized. Overall, PRP final product concentrations as well as PRP preparation protocols varied widely between systems. However, platelet concentration was directly correlated with both volume of blood collected and device centrifugal force. In conclusion, there is a large heterogeneity between PRP separation systems that must be resolved for proper study of this promising treatment.

Applications of platelet- rich plasma (prp) in contemporary pediatric dentistry

2006 ◽  
Vol 30 (4) ◽  
pp. 283-286 ◽  
Author(s):  
N. Shashikiran ◽  
V. Subba Reddy ◽  
C. Yavagal ◽  
M Zakirulla
Keyword(s):  
Growth Factors ◽  
Present Article ◽  
Bone Grafting ◽  
Platelet Rich Plasma ◽  
Case Reports ◽  
Pediatric Dentistry ◽  
Clinical Applications ◽  
Current Evidence ◽  
Invaluable Tool

Current evidence and understanding of bone science recognize the pivotal role of growth factors in all the aspects of bone grafting and regeneration. Platelet-rich-plasma (PRP) is one of the richest sources of growth factors to enhance bone regeneration. The present article aims to highlight the basic mechanisms involved in the successful use of PRP and its clinical applications in Pediatric dentistry based on our case-reports citing its use for bone grafting in young children. With pertinence to its current advantages and recent applications, PRP could soon prove to be an invaluable tool for pediatric dental surgeons worldwide.

scholarly journals EFFICACY OF PLATELET RICH PLASMA AS MONOTHERAPY IN TREATMENT OF ALOPECIA AREATA.

2021 ◽  
pp. 32-33
Author(s):  
Archana Rede ◽  
V. V. Saoji ◽  
S. N. Agrawal
Keyword(s):  
Growth Factors ◽  
Treatment Response ◽  
Alopecia Areata ◽  
Platelet Rich Plasma ◽  
Hair Follicles ◽  
Post Treatment ◽  
Immune Disease

Context: Alopecia Areata is an inflammatory auto-immune disease mainly affecting the scalp and other hair bearing areas. Platelet rich plasma (PRP) is autologous preparation of plasma with concentrated platelets which containing various growth factors and cytokines that enhance the body's inherent capacity to repair and regenerate hair follicles. Aims And Objectives: To evaluate the efficacy of platelet rich plasma as monotherapy in alopecia areata. Materials And Methods : Total 20 patients , in that 10 male and 10 female with alopecia areata will be enrolled in this study. PRP will be injected in alopecia sites (hairless patches) every 30 days for a total 4 sessions. Follow up will be taken every month for 6 months and pre and post treatment response will be noted by using photographs .

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