The Clinical Effects of Citric Acid and Laterally Positioned Pedicle Grafts in the Treatment of Denuded Root Surfaces: A Pilot Study

1980 ◽  
Vol 51 (11) ◽  
pp. 652-654 ◽  
Author(s):  
Jacob Shiloah
Keyword(s):  
Citric Acid ◽  
Pilot Study ◽  
Clinical Effects ◽  
Root Surfaces

scholarly journals Effect of Bioconditioning on Surface Characteristics of Periodontally-affected Roots of Diabetic Patients

2014 ◽  
Vol 5 (2) ◽  
pp. 81-86
Author(s):  
Hossam A Eid ◽  
Khalid M Abdelaziz ◽  
Refaat A Eid
Keyword(s):  
Citric Acid ◽  
Chronic Periodontitis ◽  
Negative Impact ◽  
Surface Characteristics ◽  
Diabetic Patients ◽  
Type Ii ◽  
Sem Images ◽  
Root Surfaces ◽  
Tetracycline Hcl

ABSTRACT Objective Advanced glycosylated end products (AGEs) in type II diabetic patients are usually precipitated on the periodontallyaffected root surfaces. The presence of periodontopathic microbes, at the same time, may also add a negative impact on the prognosis of the regenerative periodontal surgery. This in vitro study aimed to evaluate the effect of chemical conditioning on surface characteristics of periodontally-affected roots of diabetic patients. Methods Three groups (n = 25) of freshly-extracted teeth were collected from the outpatient clinics, College of Dentistry, King Khalid University. In group 1, teeth were collected from healthy individuals for orthodontic purpose. Teeth of group 2 were collected from healthy patients with chronic periodontitis, while those of group 3 were collected from diabetic patients with chronic periodontitis. Roots of the collected teeth were examined using the scanning electron microscope (SEM) before and after mechanical surface planning and chemical treatment using normal saline, EDTA gel, Tetracycline HCL (TC) or citric acid (CA) each for 4 minutes (n = 5 from each group). Results Interpretation of SEM images revealed undesirable etching effect of the chemicals used on the surfaces of healthy roots. Although EDTA showed an effective cleanse of the smear debris, it seemed to have no power on surface cuticles existed on roots of diabetic individuals. Tetracycline HCl provided acceptable conditioning of periodontally-affected root surfaces. Citric acid showed a powerful removal of both smear debris and cuticle layers off the periodontally-affected roots of diabetic individuals. Conclusion Topical application of EDTA, TC or CA shows sensible effect on the periodontally affected root surfaces. However, each of these chemicals exhibits different conditioning power. Citric acid is a promising agent to biomodify the periodontally-affected root surfaces of diabetic patients. Clinical relevance Citric acid is an acceptable biomodifier for the periodontally-affected root surfaces. This approach may improve the prognosis of periodontal therapies especially in type II diabetic patients. How to cite this article Abdelaziz KM, Eid HA, Eid RA. Effect of Bioconditioning on Surface Characteristics of Periodontally- affected Roots of Diabetic Patients. World J Dent 2014;5(2):81-86.

The Attachment of V79 and Human Periodontal Ligament Fibroblasts on Periodontally Involved Root Surfaces Following Treatment with EDTA, Citric Acid, or Tetracycline HCL: An SEM in vitro Study

2006 ◽  
Vol 7 (1) ◽  
pp. 35-43 ◽  
Author(s):  
R. Viswa Chandra ◽  
Ganesh Chandra Jagetia ◽  
K. Mahalinga Bhat
Keyword(s):  
Citric Acid ◽  
Periodontal Ligament ◽  
Chinese Hamster ◽  
Lung Fibroblasts ◽  
Control Group ◽  
Periodontal Ligament Fibroblasts ◽  
Human Periodontal Ligament Fibroblasts ◽  
Root Surfaces ◽  
Tetracycline Hcl

Abstract Objective The present in vitro study has been designed to establish and compare the effects of citric acid, EDTA, and tetracycline HCl on human periodontally diseased roots on the structure, attachment, and orientation of V79 (primary Chinese hamster lung fibroblasts) cells and human periodontal ligament fibroblasts (HPDL). Materials and Methods Commercially availableV79 cells and HPDL derived from healthy human third molars were used in this study. These fibroblasts were left in solution for seven days in order to attain confluence. Forty single-rooted teeth were obtained from patients diagnosed with periodontitis. The crown part was removed under constant irrigation and the root was split vertically into two equal halves, thus, yielding 80 specimens. Following scaling and root planing, the specimens were washed with phosphate buffered saline (PBS) and kept in 50 μg/ml gentamycin sulphate solution for 24 hours. The root pieces were then treated as follows: citric acid at pH 1, 24% EDTA, or with a 10% solution of tetracycline HCl and were then placed in V79 fibroblast cultures and HPDL cultures. The specimens were harvested after four weeks and were fixed in 2.5% glutaraldehyde in PBS before preparation for scanning electron microscopy (SEM). Results The behavior of V79 cells was similar to that of human periodontal ligament cells on root conditioned surfaces. V79 and HPDL showed a healthy morphology on root surfaces treated with citric acid and EDTA and a relatively unhealthy appearance on root surfaces treated with tetracycline HCl and distilled water (control group). Conclusion The results suggest the use of citric acid and EDTA as root conditioning agents favorably affects the migration, attachment, and morphology of fibroblasts on human root surfaces, which may play a significant role in periodontal healing and regeneration. Citation Chandra RV, Jagetia GC, Bhat KM. The Attachment of V79 and Human Periodontal Ligament Fibroblasts on Periodontally Involved Root Surfaces Following Treatment with EDTA, Citric Acid, or Tetracycline HCL: An SEM in vitro Study. J Contemp Dent Pract 2006 February;(7)1:044-059.

Comparison of Adhesion of Blood Components on Root Surfaces treated with Citric Acid, Nd:YAG, Er:YAG, and CO2 Lasers: An in vitro Analysis

2016 ◽  
Vol 6 (1) ◽  
pp. 18-23
Author(s):  
Shweta Sabnis ◽  
GV Gayathri ◽  
Khyati K Chandra ◽  
Dhoom S Mehta
Keyword(s):  
Citric Acid ◽  
Blood Cell ◽  
Nd:Yag Laser ◽  
Er:Yag Laser ◽  
Periodontal Regeneration ◽  
Control Group ◽  
Blood Components ◽  
Group I ◽  
Root Surfaces

ABSTRACT Objectives Erstwhile studies have emphasized the importance of establishing a secure fibrin linkage between the tooth-soft tissue interface for formation of a new connective attachment. Thus, periodontal regeneration is reliant on the constant adhesion, maturation and absorption of fibrin clots to the root surfaces which are compromised periodontally. Improved fibrin clot formation and blood cell attachment is being aimed by modification of the root surfaces with different agents. Limited studies have evaluated the attachment of blood cell component on various laser treated root surfaces individually. Hence, the aim of this in vitro study was to evaluate and compare the adhesion of blood components on the root surfaces treated with citric acid, Nd:YAG, Er:YAG and CO2 lasers by scanning electron microscopy (SEM). Materials and methods The proposed study was conducted on 35 root specimens (5 × 5 × 1 mm) obtained from extracted periodontally compromised permanent teeth. The root specimens were randomly divided in five groups depending upon the type of treatment rendered. Group I: Untreated control group, group II: Citric acid (pH:1), group III: Nd:YAG laser (112.5 m J/pulse), group IV: CO2 laser (12.5 J/cm2), group V: Er:YAG laser (120 m J). Following the respective treatments, fresh human whole peripheral blood obtained from a healthy donor was applied to the external surface of all root specimens. The specimens were then analysed and scored for the adhesion of the blood components with photomicrographs of SEM. Results Statistically significant increase in the adhesion of blood components was seen in all the test groups compared to control group both citric acid and Er:YAG laser showed higher adhesion of blood cells to the root surface than the Nd:YAG laser and CO2 laser. Conclusion Er:YAG laser enhanced the adhesion of blood components over the treated root surfaces. Hence, it can be safely used as a root bio-modifier ensuring stable fibrin linkage to promote periodontal regeneration. How to cite this article Sabnis S, Gayathri GV, Chandra KK, Mehta DS. Comparison of Adhesion of Blood Components on Root Surfaces treated with Citric Acid, Nd:YAG, Er:YAG, and CO2 Lasers: An in vitro Analysis. Int J Laser Dent 2016;6(1):18-23.

Pilot study on the clinical effects of dietary supplementation With Enzogenol®, a flavonoid extract of pine bark and vitamin C

2003 ◽  
Vol 17 (5) ◽  
pp. 490-494 ◽  
Author(s):  
Brett Shand ◽  
Chris Strey ◽  
Russell Scott ◽  
Zarnia Morrison ◽  
Steven Gieseg
Keyword(s):  
Pilot Study ◽  
Vitamin C ◽  
Dietary Supplementation ◽  
Pine Bark ◽  
Clinical Effects

Clinical effects of flurbiprofen tooth patch on radiation-induced oral mucositis. A pilot study

2004 ◽  
Vol 13 (1) ◽  
pp. 42-48 ◽  
Author(s):  
M. A. Stokman ◽  
F. K. L. Spijkervet ◽  
F. R. Burlage ◽  
J. L. N. Roodenburg
Keyword(s):  
Pilot Study ◽  
Oral Mucositis ◽  
Clinical Effects ◽  
Radiation Induced

The Bactericidal Effects of an Acidified Sodium Chlorite-Containing Oral Moisturizing Gel: A Pilot Study

2013 ◽  
Vol 39 (6) ◽  
pp. 689-695 ◽  
Author(s):  
Iwao Kuroyama ◽  
Shigeo Osato ◽  
Takahiro Ogawa
Keyword(s):  
Citric Acid ◽  
Pilot Study ◽  
Porphyromonas Gingivalis ◽  
Chlorine Dioxide ◽  
Soft Tissues ◽  
Low Ph ◽  
Sodium Chlorite ◽  
Chemical Dissolution ◽  
Bactericidal Effects ◽  
Chemical Disinfectant

The aim of this study was to examine the bactericidal effects and bactericidal time of an acidified sodium chlorite compound gel (ASC-Gel) on bacteria isolated from the peri-implant sulci of 10 patients who received implants 3–27 years previously, and the depth of each peri-implant sulcus was 5 mm or less. Porphyromonas gingivalis (ATCC33277) was used as the control bacterium. Five ASC-Gel preparations were created by adding 3.3%, 5.0%, 7.0%, 9.0%, and 11.0% citric acid (CA) (condition a, b, c, d, and e, respectively) into an oral moisturizing gel containing sodium chlorite. The concentrations of chlorine dioxide (ClO2) generated in ASC-Gel under conditions (a) to (e) were 12.1, 14.1, 17.2, 21.2, and 39.3 ppm, respectively. We examined the bactericidal effects of the 5 ASC-Gel preparations at volumes of 0.5, 1.0, and 2.0 mL, and measured the bactericidal time when 2.0 mL of ASC-Gel was used under condition (e). The bactericidal effects of ASC-Gel became significantly greater with increased concentrations of CA and ClO2 and with increased usage (0.5−2.0 mL) of the gel. All bacteria were killed by using 2.0 mL of ASC-Gel under condition (e). ASC-Gel also needed between 45 and 90 minutes to kill all microbes under condition (e). Within the limits of the present investigation, these results suggest that ASC-Gel is useful as a chemical disinfectant against bacteria in the peri-implant sulcus. Further studies are also required to protect teeth, the surface of hydroxyapatite-coated implants, and the surrounding soft tissues from effects of chemical dissolution such as acid erosion due to the low pH of ASC-Gel.

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