Retention of Antimicrobial Activity by Human Root Surfaces after in Situ Subgingival Irrigation with Tetracycline HCl or Chlorhexidine

AbstractThe present study describes an efficient and ecofriendly, ultrasound, one-pot click cycloaddition approach for the construction of a novel series of 1,4-disubstituted-1,2,3-triazoles tethered with fluorinated 1,2,4-triazole-benzothiazole molecular conjugates. It involved three-component condensation of the appropriate bromoacetamide benzothiazole, sodium azide and 4-alkyl/aryl-5-(2-fluorophenyl)-3-(prop-2-ynylthio)-1,2,4-triazoles4a-ethrough a Cu(I)-catalyzed 1,3-dipolar cycloaddition reaction. This approach involvesin situgeneration of azidoacetamide benzothiazole, followed by condensation with terminal alkynes in the presence of CuSO4/Na-ascorbate in aqueous DMSO under both conventional and ultrasound conditions. Some of the designed 1,2,3-triazole conjugates6a-owere recognized for their antimicrobial activity against some bacterial and fungal pathogenic strains.

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Comparative SEM Study on the Effect of Acid Etching With Tetracycline HCl or Citric Acid on Instrumented Periodontally-Involved Human Root Surfaces

Journal of Periodontology ◽

10.1902/jop.1993.64.8.689 ◽

1993 ◽

Vol 64 (8) ◽

pp. 689-693 ◽

Cited By ~ 26

Author(s):

Thomas A. Lafferty ◽

Marlin E. Gher ◽

Jonathan L. Gray

Keyword(s):

Citric Acid ◽

Acid Etching ◽

Sem Study ◽

Root Surfaces ◽

Tetracycline Hcl

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Effect of Bioconditioning on Surface Characteristics of Periodontally-affected Roots of Diabetic Patients

World Journal of Dentistry ◽

10.5005/jp-journals-10015-1264 ◽

2014 ◽

Vol 5 (2) ◽

pp. 81-86

Author(s):

Hossam A Eid ◽

Khalid M Abdelaziz ◽

Refaat A Eid

Keyword(s):

Citric Acid ◽

Chronic Periodontitis ◽

Negative Impact ◽

Surface Characteristics ◽

Diabetic Patients ◽

Type Ii ◽

Sem Images ◽

Root Surfaces ◽

Tetracycline Hcl

ABSTRACT Objective Advanced glycosylated end products (AGEs) in type II diabetic patients are usually precipitated on the periodontallyaffected root surfaces. The presence of periodontopathic microbes, at the same time, may also add a negative impact on the prognosis of the regenerative periodontal surgery. This in vitro study aimed to evaluate the effect of chemical conditioning on surface characteristics of periodontally-affected roots of diabetic patients. Methods Three groups (n = 25) of freshly-extracted teeth were collected from the outpatient clinics, College of Dentistry, King Khalid University. In group 1, teeth were collected from healthy individuals for orthodontic purpose. Teeth of group 2 were collected from healthy patients with chronic periodontitis, while those of group 3 were collected from diabetic patients with chronic periodontitis. Roots of the collected teeth were examined using the scanning electron microscope (SEM) before and after mechanical surface planning and chemical treatment using normal saline, EDTA gel, Tetracycline HCL (TC) or citric acid (CA) each for 4 minutes (n = 5 from each group). Results Interpretation of SEM images revealed undesirable etching effect of the chemicals used on the surfaces of healthy roots. Although EDTA showed an effective cleanse of the smear debris, it seemed to have no power on surface cuticles existed on roots of diabetic individuals. Tetracycline HCl provided acceptable conditioning of periodontally-affected root surfaces. Citric acid showed a powerful removal of both smear debris and cuticle layers off the periodontally-affected roots of diabetic individuals. Conclusion Topical application of EDTA, TC or CA shows sensible effect on the periodontally affected root surfaces. However, each of these chemicals exhibits different conditioning power. Citric acid is a promising agent to biomodify the periodontally-affected root surfaces of diabetic patients. Clinical relevance Citric acid is an acceptable biomodifier for the periodontally-affected root surfaces. This approach may improve the prognosis of periodontal therapies especially in type II diabetic patients. How to cite this article Abdelaziz KM, Eid HA, Eid RA. Effect of Bioconditioning on Surface Characteristics of Periodontally- affected Roots of Diabetic Patients. World J Dent 2014;5(2):81-86.

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Antimicrobial Paper Coatings Containing Microencapsulated Cymbopogon citratus Oil

Coatings ◽

10.3390/coatings9080470 ◽

2019 ◽

Vol 9 (8) ◽

pp. 470 ◽

Cited By ~ 3

Author(s):

Šumiga ◽

Ravnjak ◽

Boh Podgornik

Keyword(s):

Antimicrobial Activity ◽

In Situ Polymerization ◽

Gum Arabic ◽

Human Hand ◽

Functional Coatings ◽

Cymbopogon Citratus ◽

Multiple Exposures ◽

Citronella Oil ◽

Coated Papers

Essential oils are environmentally friendly candidates for antimicrobial smart packaging systems. Encapsulation is needed to reduce their volatility and achieve controlled release. Within this study, the essential oil of Cymbopogon citratus (citronella oil) was microencapsulated and applied in pressure-sensitive antimicrobial functional coatings on papers for secondary packaging. Two microencapsulation methods were used: complex coacervation of gelatine with carboxymethylcellulose or with gum arabic, and in situ polymerization of melamine-formaldehyde prepolymers with a polyacrylic acid modifier. Minimum inhibitory concentrations of citronella oil microcapsules were determined for Bacillus subtilis (B. subtilis), Escherichia coli (B. subtilis), Pseudomonas aeruginosa (P. aeruginosa) and Saccharomyces cerevisiae (S. cerevisiae). Microcapsule suspensions were coated on papers for flexible packaging, 2 and 30 g/m2, and mechanically activated in the weight pulling test. A novel method on agar plates in sealed Petri dishes was developed to evaluate the antimicrobial activity of released citronella vapours on E. coli and S. cerevisiae. The results showed that both microencapsulation methods were successful and resulted in a container type single-core microcapsules. In situ microcapsule suspensions had better paper coating properties and were selected for industrial settings. The antimicrobial activity of 2 g/m2 coatings was not detected; however, the antimicrobial activity of 30 g/m2 partially activated coated papers was confirmed. The product enabled a prolonged use with the gradual release of citronella oil at multiple exposures of functional papers to pressure, e.g., by a human hand during product handling.

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Repurposing of antidepression drug sertraline for antimicrobial activity against Staphylococcus aureus: a potential approach for the treatment of osteomyelitis

New Journal of Chemistry ◽

10.1039/c8nj06297h ◽

2019 ◽

Vol 43 (14) ◽

pp. 5315-5324 ◽

Cited By ~ 1

Author(s):

D. Muthu ◽

M. Gowri ◽

G. Suresh Kumar ◽

V. S. Kattimani ◽

E. K. Girija

Keyword(s):

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Precipitation Method ◽

Hydroxyapatite Nanoparticles ◽

Potential Approach ◽

Calcium Source

We report a potential approach to synthesize the repurposed sertraline drug-loaded hydroxyapatite nanoparticles using eggshell as the calcium source via the in situ precipitation method for the treatment of osteomyelitis.

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In Situ Calling Distances and High Population Independent N -Acylhomoserine Lactone-Mediated Communication on Plant Root Surfaces

Molecular Microbial Ecology of the Rhizosphere ◽

10.1002/9781118297674.ch74 ◽

2013 ◽

pp. 785-789

Author(s):

Frank B. Dazzo ◽

Stephan Gantner

Keyword(s):

Plant Root ◽

High Population ◽

Mediated Communication ◽

Acylhomoserine Lactone ◽

Root Surfaces

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Antimicrobial activity, antiaflatoxigenic potential and in situ efficacy of novel formulation comprising of Apium graveolens essential oil and its major component

Pesticide Biochemistry and Physiology ◽

10.1016/j.pestbp.2019.07.013 ◽

2019 ◽

Vol 160 ◽

pp. 102-111 ◽

Cited By ~ 10

Author(s):

Somenath Das ◽

Vipin Kumar Singh ◽

Abhishek Kumar Dwivedy ◽

Anand Kumar Chaudhari ◽

Neha Upadhyay ◽

...

Keyword(s):

Antimicrobial Activity ◽

Essential Oil ◽

Apium Graveolens

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In Situ Phylogenetic Structure and Diversity of Wild Bradyrhizobium Communities

Applied and Environmental Microbiology ◽

10.1128/aem.00667-09 ◽

2009 ◽

Vol 75 (14) ◽

pp. 4727-4735 ◽

Cited By ~ 49

Author(s):

J. L. Sachs ◽

S. W. Kembel ◽

A. H. Lau ◽

E. L. Simms

Keyword(s):

Host Species ◽

Root Surface ◽

Small Subset ◽

Root Tips ◽

Phylogenetic Structure ◽

Pure Cultures ◽

Nodulation Status ◽

Host Infection ◽

Root Surfaces

ABSTRACTBacteria often infect their hosts from environmental sources, but little is known about how environmental and host-infecting populations are related. Here, phylogenetic clustering and diversity were investigated in a natural community of rhizobial bacteria from the genusBradyrhizobium. These bacteria live in the soil and also form beneficial root nodule symbioses with legumes, including those in the genusLotus. Two hundred eighty pure cultures ofBradyrhizobiumbacteria were isolated and genotyped from wild hosts, includingLotus angustissimus,Lotus heermannii,Lotus micranthus, andLotus strigosus. Bacteria were cultured directly from symbiotic nodules and from two microenvironments on the soil-root interface: root tips and mature (old) root surfaces. Bayesian phylogenies ofBradyrhizobiumisolates were reconstructed using the internal transcribed spacer (ITS), and the structure of phylogenetic relatedness among bacteria was examined by host species and microenvironment. Inoculation assays were performed to confirm the nodulation status of a subset of isolates. Most recovered rhizobial genotypes were unique and found only in root surface communities, where little bacterial population genetic structure was detected among hosts. Conversely, most nodule isolates could be classified into several related, hyper-abundant genotypes that were phylogenetically clustered within host species. This pattern suggests that host infection provides ample rewards to symbiotic bacteria but that host specificity can strongly structure only a small subset of the rhizobial community.

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