The digestibility of biofloc meal from African catfish culture medium as a feed raw material for Pacific white shrimp

ABSTRACTThis study was conducted to evaluate the digestibility of biofloc meal collected from catfish culture as a feed raw material for Pacific white shrimp Litopenaeus vannamei diet. A basal feed with 43% crude protein content was used as a control diet and mixed with 2% of binders and 0.5 % of Cr2O3 as a marker for digestibility. The experimental diets were made by mixing 67.5% of the basal diet with 30% of biofloc meal, 2% of binders and 0.5 % of Cr2O3. Nine units of glass tanks (90 cm ×40 cm× 35 cm) filled with 100 L seawater were used as the experimental culture units. White shrimp with an average body weight of 5.61 ± 0.09 g was randomly distributed to each experimental tank at a density of 20 shrimp/tank. The feed was offered at a level of 5% shrimp biomass per day at a frequency of four times a day. The results showed that the dry matter digestibility of feed with 30% biofloc meal in shrimp were similar to that of the reference diet. However, protein and fat digestibility of feed containing biofloc meal were considerably higher than those of the reference diet. Feeding shrimp with 30% biofloc meal diet resulted in higher survival and specific growth rate and lower feed conversion ratio than those of the control. The digestibility of bioflocs dry matter, protein and lipid in Pacific white shrimp obtained in this study were 54.9%, 76.3% and 79.3%, respectively.Keywords: biofloc, digestibility, catfish, shrimp ABSTRAKPenelitian ini dilakukan untuk mengevaluasi kecernaan tepung bioflok yang diambil dari media pemeliharaan ikan lele sebagai bahan pakan udang vaname Litopenaeus vannamei. Pakan yang mengandung kadar protein 43% digunakan sebagai pakan control dan dicampur dengan 2% binder dan 0.5% Cr2O3 sebagai marker untuk kecernaan. Pakan perlakuan dibuat dengan mencampurkan 67.5% pakan control dengan 30% tepung bioflok, 2% binder dan 0.5% Cr2O3. Penelitian menggunakan sembilan unit akuarium (90 cm ×40 cm ×35 cm) yang diisi 100 L air laut. Udang vaname dengan bobot rata-rata 5.61 ± 0.09 g ditebar secara acak pada setiap akuarium perlakuan pada kepadatan 20 ekor/akuarium. Pakan diberikan dengan tingkat pemberian pakan 5% biomassa per hari sebanyak empat kali sehari. Hasil penelitian ini menunjukkan bahwa kecernaan pakan dengan 30% tepung bioflok tidak berbeda nyata dengan pakan acuan. Namun kecernaan protein dan lemak pakan yang mengandung tepung bioflok terlihat lebih tinggi daripada pakan kontrol. Pemberian pakan dengan tepung bioflok sebanyak 30% juga menghasilkan tingkat kelangsungan hidup, laju pertumbuhan spesifik udang dan rasio konversi pakan yang lebih baik daripada udang yang diberi pakan kontrol. Kecernaan bahan, protein dan lemak tepung bioflok pada udang yang didapat dalam penelitian masing-masing adalah 54.9%, 76.3% dan 79.3%.Kata-kata kunci: bioflok, ikan lele, kecernaan, udang

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Effect of a Black Soldier Fly Ingredient on the Growth Performance and Disease Resistance of Juvenile Pacific White Shrimp (Litopenaeus vannamei)

Animals ◽

10.3390/ani11051450 ◽

2021 ◽

Vol 11 (5) ◽

pp. 1450

Author(s):

Andrew Richardson ◽

João Dantas-Lima ◽

Maxime Lefranc ◽

Maye Walraven

Keyword(s):

Disease Resistance ◽

Growth Performance ◽

Litopenaeus Vannamei ◽

Control Diet ◽

Pacific White Shrimp ◽

White Shrimp ◽

Feed Conversion ◽

Black Soldier Fly ◽

Health Performance ◽

Growth Trial

This study was performed as part of developing a functional feed ingredient for juvenile Pacific white shrimp (Litopenaeus vannamei). Here we assess the effects of dietary inclusion of a Black Soldier Fly Ingredient (BSFI) from defatted black soldier fly (Hermetia illucens) larvae meal on growth performance, tolerance to salinity stress, and disease resistance when challenged with Vibrio parahaemolyticus or a strain of white spot syndrome virus (WSSV). A control diet was used for comparison with three test diets including 4.5, 7.5, and 10.5% of BSFI (BSFI4.5, BSFI7.5, and BSFI10.5). After 28 days, all diets with BSFI had improved weight gain, feed conversion ratio (FCR) and specific growth rate (SGR) compared to control. Indeed, SGR was significantly improved from inclusion of 4.5% in the diet, whilst FCR was significantly improved at 7.5% (p < 0.05). During the growth trial, survival was not affected by diet. Shrimp health performance was not significantly affected by the diets across the disease and salinity challenges. Overall, the results indicate that the inclusion of BSFI from H. illucens improves the performance of juvenile L. vannamei.

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Annual ryegrass (Lolium rigidum) seed survival and digestibility in cattle and sheep

Australian Journal of Experimental Agriculture ◽

10.1071/ea01069 ◽

2002 ◽

Vol 42 (2) ◽

pp. 111 ◽

Cited By ~ 9

Author(s):

R. Stanton ◽

J. Piltz ◽

J. Pratley ◽

A. Kaiser ◽

D. Hudson ◽

...

Keyword(s):

Dry Matter ◽

Control Diet ◽

Basal Diet ◽

Annual Ryegrass ◽

Lolium Rigidum ◽

Seed Survival ◽

Dry Matter Digestibility ◽

Cattle And Sheep

A trial was conducted to investigate the survival and digestibility of annual ryegrass (ARG) seed (Lolium rigidum L.) eaten by sheep and cattle. Sheep (n= 8) and cattle (n = 8) were fed a basal diet containing 1:1 lucerne chaff:oaten chaff with (ARG) or without (control) the inclusion of 20% total dry matter of annual ryegrass seed in a changeover design. Intake was restricted to 17 g/kg liveweight. Digestibility of the control diet was lower (P<0.01) for sheep than cattle. Annual ryegrass seed was present (P<0.01) in the faeces of both sheep and cattle within 24 h of first ingestion. Some 10.8 and 32.8% of seed ingested was excreted by sheep and cattle respectively, with 3.9% (sheep) and 11.9% (cattle) remaining germinable. Annual ryegrass seed continued to be excreted by both sheep and cattle up to 5 days after removal from the diet. Dry matter digestibility of the annual ryegrass diet was 53% in cattle.

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Apparent energy, dry matter and amino acid digestibility of differently sourced soybean meal fed to Pacific white shrimp Litopenaeus vannamei

Aquaculture Research ◽

10.1111/are.14378 ◽

2019 ◽

Vol 51 (1) ◽

pp. 326-340

Author(s):

Harsha S. C. Galkanda‐Arachchige ◽

Jingping Guo ◽

Hans H. Stein ◽

Donald Allen Davis

Keyword(s):

Amino Acid ◽

Soybean Meal ◽

Litopenaeus Vannamei ◽

Dry Matter ◽

Pacific White Shrimp ◽

White Shrimp

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Replacing Fish Meal with Defatted Insect Meal (Yellow Mealworm Tenebrio molitor) Improves the Growth and Immunity of Pacific White Shrimp (Litopenaeus vannamei)

Animals ◽

10.3390/ani9050258 ◽

2019 ◽

Vol 9 (5) ◽

pp. 258 ◽

Cited By ~ 8

Author(s):

Constant Motte ◽

Alfredo Rios ◽

Thomas Lefebvre ◽

Hong Do ◽

Morgane Henry ◽

...

Keyword(s):

Pathogenic Bacteria ◽

Fish Meal ◽

Control Diet ◽

Group Versus ◽

Tenebrio Molitor ◽

Pacific White Shrimp ◽

White Shrimp ◽

Feed Conversion ◽

Feeding Trial ◽

Yellow Mealworm

Recently, ecological and economic issues have affected fish meal (FM) supply, the main source of protein for shrimp. This triggered a search for alternative dietary protein sources for shrimp production. We studied the consequences of replacing FM with a defatted insect meal, ŸnMealTM (YM), comprised of yellow mealworm (Tenebrio molitor). Growth and immune parameters of juvenile Pacific white shrimp (Litopenaeus vannanmei) were compared after an eight-week feeding trial. Shrimp were kept in aquaria with densities of 60 and 40 shrimp/m2 and fed one of five diets in which a proportion of FM was replaced by YM. All diets were isoproteic, isoenergetic, and balanced in lysine and methionine. After the feeding trial, shrimp were challenged with pathogenic bacteria (Vibrio parahaemolyticus). Growth and feed conversion parameters improved when YM was included in shrimp diets; with the highest weight gain and best food conversion ratio (FCR) achieved when 50% of FM was replaced by YM versus the control diet that contained no YM (initial weight: 1.60 g/shrimp; growth: 5.27 vs. 3.94 g/shrimp; FCR 1.20 vs. 1.59). In challenged shrimp, mortality rates were significantly less among groups that received YM, with a 76.9% lower mortality rate in the 50% FM replacement group versus the control.

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Apparent faecal digestibility and nitrogen retention in piglets fed whole and peeled Cambodian field cricket meal

Journal of Insects as Food and Feed ◽

10.3920/jiff2017.0019 ◽

2017 ◽

Vol 3 (4) ◽

pp. 279-288 ◽

Cited By ~ 6

Author(s):

P. Miech ◽

J.E. Lindberg ◽

Å. Berggren ◽

T. Chhay ◽

A. Jansson

Keyword(s):

Body Weight ◽

Dry Matter ◽

Block Design ◽

Control Diet ◽

Nitrogen Retention ◽

Field Cricket ◽

Feed Conversion ◽

Crude Fibre ◽

Dry Matter Digestibility ◽

Dietary Treatments

This study evaluated diets including whole or peeled (legs removed) crickets (Teleogryllus testaceus) in terms of diet digestibility, growth and nitrogen retention, using pigs as an animal model. The experiment included three iso-nitrogenous diets (18.4% crude protein) including either whole cricket meal (WC), body cricket meal (legs removed, BC) or fish meal (control) as the main protein source. Castrated male piglets (n=21, 30-45 days) with initial body weight 13.0±0.3 kg were allocated to one of the dietary treatments (7 piglets/treatment) in a fixed block design. The piglets were kept in single bamboo/wooden stalls with slatted floors and were adapted to the feeds and the housing for 5 days before starting the 25-day experiment. The diets were offered ad libitum, but close to appetite (approximately 5% of body weight). Feed intake was recorded and piglets were weighed every 5 days. During days 20-25, total collection of faeces and urine was performed. Dry matter and nutrient intake were higher for piglets fed the WC and BC diets than for those fed the control diet. From day 10, piglets fed BC and WC were heavier than piglets fed the control diet, but there were no differences between WC and BC. Dry matter digestibility was highest for diet WC, and ash, crude fibre and crude fat digestibility was higher for BC and WC than for the control diet. Feed conversion ratio was lower for the WC and BC diets than for the control diet, and nitrogen retention (% of digested) was higher. We concluded that field cricket meal is a nutritious feedstuff for mono-gastric animals, and most likely also for humans. Removal of legs did not facilitate or improve the digestibility values and nitrogen retention. Thus, in order to minimise food waste, crickets should not be peeled in this way if they are going to be processed into meal.

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Mixed culture of Pacific white shrimp Litopenaeus vannamei (Boone, 1931) and flathead grey mullet Mugil cephalus (Linnaeus, 1758) in floating cages

Indian Journal of Fisheries ◽

10.21077/ijf.2016.63.3.56378-08 ◽

2016 ◽

Vol 63 (3) ◽

Cited By ~ 1

Author(s):

Shubhadeep Ghosh ◽

Ritesh Ranjan ◽

Sekar Megarajan ◽

Phalguni Pattnaik ◽

Biswajit Dash ◽

...

Keyword(s):

Mixed Culture ◽

Litopenaeus Vannamei ◽

Pacific White Shrimp ◽

Mugil Cephalus ◽

White Shrimp ◽

Average Weight ◽

Uniform Density ◽

Grey Mullet ◽

Feed Conversion ◽

Average Production

The study explored the possibility of integrating the grey mullet Mugil cephalus (Linnaeus, 1758) along with Pacific white shrimp Litopenaeus vannamei (Boone, 1931) in floating cage culture in Godavary Estuary, India. Post-larvae (PL 12) of L. vannamei (3 lakhs nos.), were acclimatised and nursed in five hapas for 28 days at a density of 3333 nos. m-2, with survival of 60%. L. vannamei juveniles having mean weight of 0.86 g, stocked at a density of 1060 nos. m-2, were cultured along with and without pre-stocked M. cephalus, in three floating cages each. Six thousand fry of M. cephalus (mean length 4.17 cm; mean weight 1.22 g) were stocked in three cages at uniform density of 23.5 nos. m-3, three months prior to stocking of L. vannamei. Shrimps were fed commercial pellets @ 3-8% of body weight, four times daily and harvested after 68 days. Fishes were fed with pelleted feed and after five months attained mean length of 23.7 cm and mean weight of 274.1 g. Survival was 46.4% and the average production obtained was 250.2 kg. At harvest, L. vannamei in monoculture system attained mean weight of 13.3 g and in the mixed culture system, average weight obtained for the shrimps was 13.5 g. Survival, feed conversion ratio (FCR) and production of L. vannamei from monoculture were 64.7%, 2.0 and 258.9 kg and from mixed culture 76.8%, 1.6 and 311.5 kg respectively. Daily weight increment and specific growth rate (SGR) of L. vannamei was 0.18 g and 4.06 for monoculture and 0.19 g and 4.01 for mixed culture, resepectively. Feed conversion, survival and production of L. vannamei were significantly (p<0.05) better in mixed culture, confirming technical superiority of mixed culture over monoculture.

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Molecular effects of mannanase-hydrolyzed coprameal to intestinal immunity in the Pacific white shrimp (Litopenaeus vannamei)

10.1101/2021.02.16.431529 ◽

2021 ◽

Author(s):

Wanilada Rungrassamee ◽

Sopacha Arayamethakorn ◽

Nitsara Karoonuthaisiri ◽

Shih-Chu Chen ◽

Eric Chang ◽

...

Keyword(s):

Immune System ◽

Litopenaeus Vannamei ◽

Control Diet ◽

Pacific White Shrimp ◽

White Shrimp ◽

Control Group ◽

Intestinal Immunity ◽

Toll Like Receptor ◽

Transcript Levels ◽

Significant Difference

AbstractTo mitigate disease outbreak, an alternative approach through enhancing shrimp immunity was explored. Mannan oligosaccharides (MOS) have been previously reported to enhance shrimp immune system. Here, coprameal samples were digested with mannanase to yield MOS, namely, mannanase-hydrolyzed coprameal (MCM) and feasibility of MCM as shrimp immunostimulant in grow-out ponds was determined. Pacific white shrimp (Litopenaeus vannamei) were fed with the commercial diet containing 1% MCM as the MCM-supplemented group and compared to the non-MCM control diet. There was no significant difference in survival rates between the MCM-supplemented and the control groups throughout the 4-month-period of the trial (p > 0.05). Gene expression analysis in shrimp intestines revealed that the transcript levels of antimicrobial peptides (anti-lipopolysaccharide factor isoform 1 (alf1), penaeidin (pen3a) and crustin (crus)) and lysozymes (lyz) were not significantly different in the MCM-supplemented group. Meanwhile, C-type lectin and toll-like receptor transcript levels, whose gene products play roles as pattern recognition proteins, were significantly higher in a group fed with MCM for 2- and 4-month periods than those of the control group (p < 0.05). The increased transcript levels of C-type lectin and toll-like receptor provide evidence for potential implementation of MCM as feed supplement to modulate shrimp immune system.

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Functional Feed Assessment on Litopenaeus vannamei Using 100% Fish Meal Replacement by Soybean Meal, High Levels of Complex Carbohydrates and Bacillus Probiotic Strains

Marine Drugs ◽

10.3390/md9061119 ◽

2011 ◽

Vol 9 (6) ◽

pp. 1119-1132 ◽

Cited By ~ 46

Author(s):

Jorge Olmos ◽

Leonel Ochoa ◽

Jesus Paniagua-Michel ◽

Rosalia Contreras

Keyword(s):

Stress Tolerance ◽

Litopenaeus Vannamei ◽

Probiotic Strain ◽

Pacific White Shrimp ◽

Conversion Ratio ◽

White Shrimp ◽

Feed Conversion ◽

Low Oxygen ◽

Commercial Feed ◽

Probiotic Strains

Functional feed supplemented with alternative-economic nutrient sources (protein, carbohydrates, lipids) and probiotics are being considered in shrimp/fish aquaculture production systems as an option to increase yield and profits and to reduce water pollution. In this study the probiotic potential to formulate functional feeds have been evaluated using four dietary treatments: Treatment 1 (B + Bs); Bacillus subtilis potential probiotic strain was supplemented to a soybeanmeal (SBM)—carbohydrates (CHO) basal feed. Treatment 2 (B + Bm); Bacillus megaterium potential probiotic strain was supplemented to the same SBM-CHO basal feed. In Treatment 3 (B); SBM-CHO basal feed was not supplemented with probiotic strains. Treatment 4 (C); fishmeal commercial feed (FM) was utilized as positive control. Feeding trials evaluated the survival, growth, and food conversion ratio and stress tolerance of juvenile Litopenaeus vannamei (Boone) Pacific white shrimp. Best overall shrimp performance was observed for animals fed with Treatment 1 (B+Bs); additionally, stress tolerance and hemolymph metabolites also showed the best performance in this treatment. SBM-CHO basal feed not supplemented with probiotic strains (B) presented smaller growth and lower feed conversion ratio (FCR). Shrimps fed with the fishmeal commercial feed (C) presented the lowest stress tolerance to high ammonia and low oxygen levels. Specifically selected B. subtilis strains are recommended to formulate functional and economical feeds containing high levels of vegetable; protein and carbohydrates as main dietary sources in L. vannamei cultures.

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Effectiveness of sinbiotic at different doses in Pacific white shrimp pond culture

Jurnal Akuakultur Indonesia ◽

10.19027/jai.14.1-8 ◽

2015 ◽

Vol 14 (1) ◽

pp. 1

Author(s):

, Sukenda ◽

Rizki Praseto ◽

, Widanarni

Keyword(s):

Litopenaeus Vannamei ◽

Culture System ◽

Pacific White Shrimp ◽

White Shrimp ◽

Control Treatment ◽

Intensive Culture ◽

Feed Conversion ◽

Ratio Size ◽

Increasing Demand ◽

And Control

ABSTRACT The increasing demand of white shrimp Litopenaeus vannamei requires the application of intensive culture system. However, intensive culture system of white shrimp could increase the risk of disease outbreak. The application of sinbiotic may provide solution to the problem. This study was aimed to evaluate the effectiveness of the use of technical sinbiotic on the survival and growth of white shrimp in ponds. This study consisted of four treatments; treatment K (control), treatment A (probiotic 0.5% and prebiotic 1%), treatment B (probiotic 1% and prebiotic 2%), and treatment C (probiotic 2% and prebiotic 4%). The results showed that administration of sinbiotic had no significant differences on survival rate, growth rate, feed conversion ratio, size, and biomass of shrimp (P>0.05). However, based on analysis of business, sinbiotic A provided higher profits to the farmer (Rp10.230) compared to other symbiotic treatments and control. Keywords: Litopenaeus vannamei, sinbiotic, technical media ABSTRAK Permintaan terhadap udang vaname Litopenaeus vannamei yang semakin meningkat membuat sistem budidaya udang sebaiknya menggunakan sistem budidaya intensif. Namun demikian sistem intensif pada budidaya udang vaname dapat meningkatkan risiko timbulnya penyakit. Penggunaan sinbiotik diharapkan dapat memberikan solusi dalam mengatasi masalah tersebut. Penelitian ini bertujuan untuk menguji efektivitas penggunaan sinbiotik teknis terhadap sintasan, pertumbuhan, dan keuntungan usaha udang vaname yang dipelihara di tambak. Penelitian ini terdiri atas empat perlakuan, yaitu perlakuan K (kontrol), perlakuan A (probiotik 0,5% dan prebiotik 1%), perlakuan B (probiotik 1% dan prebiotik 2%), dan perlakuan C (probiotik 2% dan prebiotik 4%). Hasil penelitian menunjukkan bahwa pemberian sinbiotik tidak berbeda nyata dalam nilai sintasan, laju pertumbuhan, rasio konversi pakan, size, dan biomassa udang (P>0,05). Namun demikian, berdasarkan analisis usaha perlakuan A memberikan keuntungan lebih tinggi (Rp10.230) dibandingkan dengan perlakuan sinbiotik lainnya serta kontrol. Kata kunci: Litopenaeus vannamei, sinbiotik, media teknis

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Digestibility of insect meals for Pacific white shrimp (Litopenaeus vannamei) and their performance for growth, feed utilization and immune responses

PLoS ONE ◽

10.1371/journal.pone.0260305 ◽

2021 ◽

Vol 16 (11) ◽

pp. e0260305

Author(s):

Jaehyeong Shin ◽

Kyeong-Jun Lee

Keyword(s):

Fatty Acids ◽

Immune Responses ◽

Litopenaeus Vannamei ◽

Fish Meal ◽

Control Diet ◽

Saturated Fatty Acids ◽

Pacific White Shrimp ◽

White Shrimp ◽

Black Soldier Fly ◽

Amino Acid Availability

This study was conducted to examine digestibility of insect meals for Pacific white shrimp (Litopenaeus vannamei) and their utilization as fish meal substitutes. The tested insect meals were mealworm, silkworm, black soldier fly, rice grasshopper, two-spotted cricket, dynastid beetle and white-spotted flower chafer. Apparent digestibility coefficients of the tested insect meals were 83–89% for protein, 91–98% for lipid, 84–90% for energy, 77–81% for dry matter, 28–36% for chitin, 76–96% for amino acids and 89–93% for fatty acids. The amino acid availability of insect meals was high in taurine (93–96%), arginine (91–95%) and lysine (90–95%). Availability of fatty acids were 89–93% for saturated fatty acids, 90–93% for monounsaturated fatty acids and 88–93% for polyunsaturated fatty acids. For a feeding trial, a control diet was formulated using 27% tuna byproduct meal as a fish meal source and seven other diets were prepared replacing 10% tuna byproduct meal in the control diet with each insect meal. Triplicate groups of shrimp (initial body weight: 0.17 g) were fed the diets for 65 days. The growth performance was significantly improved when the shrimp were fed black soldier fly or dynastid beetle included diet. Dietary supplementation of insect meals significantly improved non-specific immune responses and antioxidant enzyme activity in the shrimp. These results indicate that the tested insect meals have high potentials to be used as a protein source that could replace fish meal in diets for the shrimp.

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