scholarly journals Characteristic of no-synthase of peripheral blood lymphocytes of patients with rheumatic pathology

2015 ◽  
Vol 84 (1) ◽  
pp. 46-54
Author(s):  
Ulyana Efremova ◽  
Nataliya Lychkovska ◽  
Roman Fafula ◽  
Zinoviy Vorobets
Keyword(s):  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
No Synthase ◽  
Kinetic Properties ◽  
No Production ◽  
Autoimmune Process ◽  
Blood Lymphocytes ◽  
Nadph Oxidation ◽  
The Difference

It is known that NO is a ubiquitous mediator which acts as a universal modulator of various functions in organism and is produced by three isoforms of NO synthase. Nowadays the role of NO in the development of autoimmune diseases is actively studied. However, it remains unclear the biochemical and biophysical mechanisms of disturbances of NOS activity in blood lymphocytes at autoimmune process. The aim of present work is to study the kinetic properties of NO-synthase of peripheral blood lymphocytes of patients with rheumatic pathology. The study was carried out on peripheral blood lymphocytes isolated from patients with rheumatoid arthritis and ankylosing spondylitis. NOS activity was determined on the saponin-permeabilized blood lymphocytes. The difference between the values of NADPH oxidation with L-Arg and with inhibitor L-NAME reflects the value of the NADPH oxidation, ie total NOS activity. The kinetic properties of NO-synthase in peripheral blood lymphocytes of patients with rheumatic pathology were studied. It was found that the development of rheumatic pathology is associated with an imbalance in the NO synthesis and changes of kinetic parameters of NOS. It was shown that reduction in eNOS activity is accompanied by a sharp increase in activity of its inducible form. It was established that inhibition of eNOS occurs by noncompetitive type. NO production in lymphocytes of patients with rheumatic diseases is mainly realized by iNOS, whereas under normal physiological conditions endothelial form of the enzyme is being involved.

Mechanism of cadmium induced apoptosis in human peripheral blood lymphocytes: The role of p53, Fas and Caspase-3

2013 ◽  
Vol 36 (3) ◽  
pp. 1033-1039 ◽  
Author(s):  
Abdullah H. Al-Assaf ◽  
Ali M. Alqahtani ◽  
Ali A. Alshatwi ◽  
Naveed A. Syed ◽  
Gowhar Shafi ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Caspase 3 ◽  
Human Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes ◽  
Human Peripheral Blood Lymphocytes ◽  
Induced Apoptosis

Low frequency of BCL-2/JH translocation in peripheral blood lymphocytes of healthy Japanese individuals

Blood ◽  
2001 ◽  
Vol 98 (2) ◽  
pp. 486-488 ◽  
Author(s):  
Masaki Yasukawa ◽  
Shiro Bando ◽  
Gottfried Dölken ◽  
Eiji Sada ◽  
Yoshihiro Yakushijin ◽  
...  
Keyword(s):  
Follicular Lymphoma ◽  
Peripheral Blood ◽  
Low Frequency ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes ◽  
Japanese Adults ◽  
Japanese General Population ◽  
The Difference ◽  
Follicular Lymphomas ◽  
Immunoglobulin Heavy Chain Genes

The incidence of follicular lymphoma differs significantly between white and Japanese individuals. Translocation between theBCL-2 and immunoglobulin heavy chain genes is detected in 85% to 90% of all follicular lymphomas in whites. Recently,BCL-2/JH translocation was detected in peripheral blood lymphocytes from more than 50% of healthy white individuals. To clarify the reason for the difference in incidence of follicular lymphoma between whites and Japanese, the frequency ofBCL-2/JH translocation in peripheral blood lymphocytes of healthy Japanese individuals was compared with that of German individuals. The prevalence of BCL-2/JHtranslocation in Japanese adults appeared to be significantly lower than that in German adults. The present data suggest that the low frequency of BCL-2/JH translocation in the Japanese general population may be one of the major reasons for the difference in incidence of follicular lymphoma between whites and Japanese.

scholarly journals CSF-1-Induced DC-SIGN+ Macrophages Work In The Ovarian Endometriosis

2021 ◽  
Author(s):  
Xiaocui Li ◽  
Wei Hong ◽  
Yunlang Cai ◽  
Zhenzhen Zheng ◽  
Min An
Keyword(s):  
Peripheral Blood ◽  
Peritoneal Fluid ◽  
Immune Status ◽  
Biological Activities ◽  
Peripheral Blood Lymphocytes ◽  
Enzyme Linked Immunosorbent Assay ◽  
Predominant Role ◽  
Blood Lymphocytes ◽  
Suppressive Phenotype

Abstract BackgroundCSF-1 was found to be accumulated in the lesions and peritoneal fluid of endometriosis patients, and CSF-1 induced THP-1-derived macrophages to polarize toward a suppressive phenotype. Researchers found that macrophages were the predominant cells in the peritoneal fluid (PF) of endometriosis patients, and the primary consensus is that the immune status in the PF of endometriosis patients exhibits a depressed state. Does the cytokine CSF-1 induce monocytes to differentiate into macrophages with a DC-SIGN+ suppressive phenotype in endometriosis?MethodsThe level of CSF-1 in control endometrium (N=11), eutopic endometrium (N=17), and ectopic (N=39) endometrium of endometriosis patients was evaluated by real-time polymerase chain reaction and in the PF of control (N=25) and endometriosis (N=35) patients by enzyme-linked immunosorbent assay. CSF-1 was examined by a MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel in an in vivo study. DC-SIGN+ suppressive macrophages were detected by immunohistochemical staining of tissues and flow cytometric analysis of the PF of control (N=25) and endometriosis (N=35) patients. The phenotypes and biological activities of the resulting macrophages derived from THP-1 cells induced by CSF-1 were compared by an in vitro coculture system with peripheral blood lymphocytes from normal subjects.Results In this study, we found the proportion of DC-SIGN+ suppressive macrophages was larger in the abdominal immune microenvironment of endometriosis patients. CSF-1 was primarily secreted from the ectopic lesions and peritoneum of mice with endometriosis. And, CSF-1 induced the polarization of macrophages toward a DC-SIGN+ suppressive phenotype; this effect was abolished by the addition of anti-CSF-1R. CSF-1 induced DC-SIGN+ macrophages, leading to a depressed status of peripheral blood lymphocytes, including a high percentage of Treg cells and a low percentage of CD8+ T cells. Similarly, blockade with anti-CSF-1R abrogated this biological effect. This is the first study on the predominant role of DC-SIGN+ suppressive macrophages in the depressed immune status of endometriosis patients.Conclusions This is the first study on the predominant role of DC-SIGN+ suppressive macrophages in the depressed immune status of endometriosis patients. Further study of the mechanism and biological activities of CSF-1-induced DC-SIGN+ suppressive macrophages will enhance our understanding of the physiology of endometriosis and indicate new directions for further study.

scholarly journals Osmotic fragility of peripheral blood lymphocytes in chronic lymphatic leukemia and malignant lymphoma

Blood ◽  
1978 ◽  
Vol 51 (4) ◽  
pp. 645-651 ◽  
Author(s):  
P Resnitzky ◽  
N Reichman
Keyword(s):  
Malignant Lymphoma ◽  
Peripheral Blood ◽  
Osmotic Fragility ◽  
Peripheral Blood Lymphocytes ◽  
Chronic Lymphatic Leukemia ◽  
Control Groups ◽  
Blood Lymphocytes ◽  
Lymphatic Leukemia ◽  
Additional Diagnostic Parameter ◽  
The Difference

Abstract The osmotic fragility (OF) of peripheral blood lymphocytes from patients with chronic lymphatic leukemia (CLL) and non-Hodgkin malignant lymphoma (ML) was investigated employing an automatic recording method and compared with that of lymphocytes from healthy subjects and from patients suffering from various non-neoplastic diseases. The curves from CLL and ML showed a pattern of increased lymphocyte OF compared with those of the two control groups, and the difference was statistically significant ( less than 0.001). In CLL the increase in OF was more pronounced than in ML, and the shape of the curve was different from that in the other groups. The employment of peripheral blood lymphocyte OF as an additional diagnostic parameter in the diagnosis of CLL and ML is suggested.

scholarly journals The kinetic properties of the ecto-ATPase of human peripheral blood lymphocytes and of chronic lymphatic leukemia cells

Blood ◽  
1983 ◽  
Vol 62 (5) ◽  
pp. 1041-1046
Author(s):  
HR Gutmann ◽  
YM Chow ◽  
RL Vessella ◽  
B Schuetzle ◽  
ME Kaplan
Keyword(s):  
B Cell ◽  
Peripheral Blood ◽  
Specific Activity ◽  
Human Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Kinetic Constants ◽  
Kinetic Properties ◽  
Blood Lymphocytes ◽  
Significant Difference ◽  
Specific Activities

This study examines whether the activity of the Mg2+-dependent ecto- ATPase of the surface membrane of the human lymphocyte is changed in chronic lymphocytic B-cell leukemia (CLL-B) and may be an indicator of malignant transformation. The ecto-ATPase activities of preparations consisting predominantly of T or B cells were compared to each other and to the ecto-ATPase of the CLL peripheral blood lymphocytes (PBL). The specific activities and kinetic constants of the ecto-ATPase of the cell preparations were determined with [gamma-32P] adenosine triphosphate (ATP) as substrate. B-enriched lymphocytes had nearly fourfold greater specific activity and apparent Vmax than T-enriched lymphocytes, while the Km values of both cell types showed no significant difference. The specific activities and kinetic constants of the ecto-ATPase of the CLL PBL were significantly higher than the corresponding values of PBL or of B-enriched lymphocytes. Judging from the kinetic constants the ecto-ATPase of the CLL-B lymphocyte appears to be an enzyme that is distinctly different from that of the normal B cell. On the basis of the kinetic properties, the ecto-ATPase of the B cell appears to be identical with that of the T cell. The differences in the maximal velocities of the hydrolysis of ATP by B and T cells are likely due to a greater number of enzymatic sites on the B cell.

scholarly journals In vitro analysis of the effect of Go Ark on Human Peripheral Blood Lymphocytes

2020 ◽  
Vol 11 (3) ◽  
pp. 410-414
Author(s):  
Daya Shankar Gautam ◽  
Prahlad Marskole ◽  
Saraswati Mishra ◽  
Nisha Tiwari ◽  
Anjali Kumari ◽  
...  
Keyword(s):  
Cell Viability ◽  
Peripheral Blood ◽  
Human Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Blood Lymphocytes ◽  
Human Peripheral Blood Lymphocytes ◽  
The Difference ◽  
Vitro Analysis ◽  
In Vitro Analysis

Cow is worshiped in India as “Gomata” since ancient time. Its values have been signified in Vedas, Puranas & Ayurveda. Its urine/Go Ark is used in rituals & medicines traditionally in India. The Significance of Cow Urine has been studied by many workers. Now it is available in the market as distillate. Hence this study was designed to assess the potential of Fresh Go Ark (FGA) and Distillate Go Ark (DGA) on Human Peripheral blood lymphocytes (PBL) in Vitro using MTT Assay. It was found that FGA & DGA both had the potential to enhance the cell viability of Human PBL. FGA showed greater potential towards the enhancement of cell viability on Human PBL than that of DGA. However the difference between the impacts of FGA & DGA was not found to be significant when tested through Two way ANOVA.

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