scholarly journals Potential value of serum Aspergillus IgG antibody detection in the diagnosis of invasive and chronic pulmonary aspergillosis in non-agranulocytic patients

2020 ◽  
Author(s):  
Qihong Yu ◽  
Jingdong He ◽  
Bin Xing ◽  
Xin Li ◽  
Hongyu Qian ◽  
...  
Keyword(s):  
Bacterial Pneumonia ◽  
Acute Infection ◽  
Roc Curves ◽  
Antibody Detection ◽  
Healthy Controls ◽  
Igg Antibody ◽  
Pulmonary Aspergillosis ◽  
Igm Antibody ◽  
Positive Rate ◽  
Chronic Pulmonary Aspergillosis

Abstract Background: At present, serum Aspergillus IgG and IgM antibody detection is mainly used in the diagnosis of chronic pulmonary aspergillosis (CPA), but its value in the diagnosis of invasive pulmonary aspergillosis (IPA) in non-agranulocytic patients is still unclear. IgM can be used as a marker of acute infection to help diagnose acute infection-related diseases. IgG is a marker of long-term infection and is used to assist in the diagnosis of pre-existing or chronic infection-related diseases. The aim of this study was to investigate and compare the value of serum Aspergillus IgG and IgM antibody detection in the diagnosis of IPA and CPA in non-agranulocytic patients.Methods: Fifty-eight cases of pulmonary aspergillosis (37 IPA and 21 CPA cases), 15 cases of community-acquired bacterial pneumonia and 50 cases in the healthy control group were collected. The serum (1,3)-β-D-glucan test (G test) was performed with a chromogenic method, and the galactomannan test (GM test) and Aspergillus IgG and IgM antibody detection were performed by commercial enzyme-linked immunosorbent assay (ELISA) in all patients. The sensitivity and specificity, cut-off value and area under the curve (AUC) of Aspergillus IgG and IgM antibodies were further obtained by receiver operating characteristic (ROC) curves.Results: The positive rate of the G test, Aspergillus IgG antibody detection and the GM test also showed notable differences among the IPA, CPA, community-acquired bacterial pneumonia and healthy groups (P = 0.006, P < 0.001 and P = 0.217, respectively). Only the positive rate of the GM test showed a significant difference between the IPA and CPA groups (P = 0.04). ROC curves indicated that Aspergillus IgG antibody detection had a higher specificity in the IPA group than in the CPA group (0.952). The detection of Aspergillus IgG antibody can preferably distinguish IPA from community-acquired bacterial pneumonia and healthy controls (sensitivity = 0.923, specificity = 0.459, cut-off value = 134.46, AUC = 0.727). It can also distinguish CPA from community-acquired bacterial pneumonia and healthy controls (sensitivity = 0.952, specificity = 0.692, cut-off value = 75.46, AUC = 0.873).Conclusions: Serum Aspergillus IgG antibody detection may have certain clinical value in the diagnosis of IPA and CPA in non-agranulocytic patients.

scholarly journals Diagnostic value of serum Aspergillus IgG antibody for invasive pulmonary aspergillosis and chronic pulmonary aspergillosis in non-agranulocytic patients

2020 ◽  
Author(s):  
Qihong Yu ◽  
Jingdong He ◽  
Bin Xing ◽  
Xin Li ◽  
Hongyu Qian ◽  
...  
Keyword(s):  
Bacterial Pneumonia ◽  
Invasive Pulmonary Aspergillosis ◽  
Roc Curves ◽  
Antibody Detection ◽  
Healthy Controls ◽  
Igg Antibody ◽  
Pulmonary Aspergillosis ◽  
Igm Antibody ◽  
Positive Rate ◽  
Chronic Pulmonary Aspergillosis

Abstract Background: At present, serum Aspergillus IgG and IgM antibody detection is mainly used in the diagnosis of chronic pulmonary aspergillosis (CPA), but its value in the diagnosis of invasive pulmonary aspergillosis (IPA) in non-agranulocytic patients is still unclear. IgG is a marker of long-term infection and is used to assist in the diagnosis of pre-existing or chronic infection-related diseases. The aim of this study was to investigate and compare the value of serum Aspergillus IgG and IgM antibody detection in the diagnosis of IPA and CPA in non-agranulocytic patients. Methods: Fifty-eight cases of pulmonary aspergillosis (37 IPA and 21 CPA cases), 15 cases of community-acquired bacterial pneumonia and 50 cases in the healthy control group were collected. The serum (1,3)-β-D-glucan test (G test) was performed with a chromogenic method, and the galactomannan test (GM test) and Aspergillus IgG and IgM antibody detection were performed by commercial enzyme-linked immunosorbent assay (ELISA) in all patients. The sensitivity and specificity, cut-off value and area under the curve (AUC) of Aspergillus IgG and IgM antibodies were further obtained by receiver operating characteristic (ROC) curves. Results: The positive rate of the G test, Aspergillus IgG antibody detection and the GM test also showed notable differences among the IPA, CPA, community-acquired bacterial pneumonia and healthy groups ( P = 0.006, P < 0.001 and P = 0.217, respectively). Only the positive rate of the GM test showed a significant difference between the IPA and CPA groups ( P = 0.04). ROC curves indicated that Aspergillus IgG antibody detection had a higher specificity in the IPA group than in the CPA group (0.952). The detection of Aspergillus IgG antibody can preferably distinguish IPA from community-acquired bacterial pneumonia and healthy controls (sensitivity = 0.923, specificity = 0.459, cut-off value = 134.46, AUC = 0.727). It can also distinguish CPA from community-acquired bacterial pneumonia and healthy controls (sensitivity = 0.952, specificity = 0.692, cut-off value = 75.46, AUC = 0.873). Conclusions: Serum Aspergillus IgG antibody detection may have certain clinical value in the diagnosis of IPA and CPA in non-agranulocytic patients.

scholarly journals Diagnostic value of serum Aspergillus IgG antibody for invasive pulmonary aspergillosis and chronic pulmonary aspergillosis in non-agranulocytic patients

2019 ◽  
Author(s):  
Qihong Yu ◽  
Jingdong He ◽  
Bin Xing ◽  
Xin Li ◽  
Hongyu Qian ◽  
...  
Keyword(s):  
Bacterial Pneumonia ◽  
Acute Infection ◽  
Invasive Pulmonary Aspergillosis ◽  
Roc Curves ◽  
Diagnostic Value ◽  
Healthy Controls ◽  
Igg Antibody ◽  
Pulmonary Aspergillosis ◽  
Positive Rate ◽  
Chronic Pulmonary Aspergillosis

Abstract Background: At present, serum Aspergillus IgG and IgM antibodies are mainly used in the diagnosis of chronic pulmonary aspergillosis (CPA), but their diagnostic value in invasive pulmonary aspergillosis (IPA) in non-agranulocytic patients is still unclear. IgM can be used as a marker of acute infection to help diagnosing acute infection-related diseases. IgG is a marker of long-term infection and used to assist in the diagnosis of preexisting or chronic infection-related diseases. The aim of this study was to investigate and compare the value of serum Aspergillus IgG and IgM antibody detection in diagnosis of IPA and CPA in non-agranulocytic patients. Methods: 58 cases of pulmonary aspergillosis (37 IPA and 21 CPA cases), 15 cases of community-acquired bacterial pneumonia and 50 cases of healthy control group were collected. Serum (1,3)-β-D-glucan test (G test) was performed with a chromogenic method, and galactomannan test (GM test), Aspergillus IgG and IgM antibody were detected by commercial enzyme-linked immunosorbent assay (ELISA) in all patients. The sensitivity and specificity, cut off value and the area under curve (AUC) of Aspergillus IgG and IgM antibodies were further obtained by the receiver operating characteristic (ROC) curves. Results: The positive rate of G test, Aspergillus IgG antibody and GM test also showed notably differences among IPA, CPA, community-acquired bacterial pneumonia and healthy groups (P = 0.006, P < 0.001 and P = 0.217, respectively). Only the positive rate of GM test statistically distinct difference between IPA and CPA groups (P = 0.04). ROC curves indicated that Aspergillus IgG antibody with the highest specificity when IPA group compared with CPA groups (0.952). Aspergillus IgG antibody can preferably distinguish IPA from community-acquired bacterial pneumonia and healthy controls (sensitivity = 0.923, specificity = 0.459, cut off value = 134.46, AUC = 0.727). It can also distinguish CPA from community-acquired bacterial pneumonia and healthy controls (sensitivity = 0.952, specificity = 0.692, cut off value = 75.46, AUC = 0.873). Conclusions: Serum Aspergillus IgG has certain clinical value in the diagnosis of IPA and CPA in non-agranulocytic patients.

scholarly journals Diagnostic value of serum Aspergillus IgG antibody for pulmonary aspergillosis in non-agranulocytic patients

2019 ◽  
Author(s):  
Qihong Yu ◽  
Jingdong He ◽  
Bin Xing ◽  
Xin Li ◽  
Hongyu Qian ◽  
...  
Keyword(s):  
Bacterial Pneumonia ◽  
Invasive Pulmonary Aspergillosis ◽  
Diagnostic Value ◽  
Antibody Detection ◽  
Control Group ◽  
Igg Antibody ◽  
Pulmonary Aspergillosis ◽  
Healthy Group ◽  
Igm Antibodies ◽  
Igm Antibody

Abstract Background: At present, serum Aspergillus IgG and IgM antibodies are mainly used in the diagnosis of chronic pulmonary aspergillosis (CPA), but the diagnostic value in invasive pulmonary aspergillosis (IPA) in non-agranulocytic patients is still unclear. The aim of this study was to investigate the diagnostic value of serum Aspergillus IgG and IgM antibody detection for pulmonary aspergillosis in non-agranulocytic patients.. Methods: 58 cases of pulmonary aspergillosis (37 IPA and 21 CPA cases), 15 cases of bacterial pneumonia and 50 cases of healthy control group were collected. Serum G test and GM test were performed, and Aspergillus IgG and IgM antibody were detected in all patients. The sensitivity and specificity, cut off value and AUC of Aspergillus IgG and IgM antibodies were further obtained by the ROC curves. Results: The positive rates of G test and Aspergillus IgG antibody in pulmonary aspergillosis group were significantly higher compared with bacterial pneumonia group or healthy group (P = 0.015 and P < 0.0001, respectively). Aspergillus IgG antibody can preferably distinguish CPA from bacterial pneumonia and healthy controls (sensitivity = 0.952, specificity = 0.692, cut off value = 75.46, AUC = 0.873). However, the performance of it was poor in distinguishing IPA from CPA, healthy group and pneumonia group (AUC < 0.7). Conclusions: Serum Aspergillus IgG has certain clinical value in the diagnosis of pulmonary aspergillosis in non-agranulocytic patients.

scholarly journals Exhaled Volatile Organic Compounds for Identifying Patients With Chronic Pulmonary Aspergillosis

2021 ◽  
Vol 8 ◽  
Author(s):  
Zheng-Tu Li ◽  
Pei-Ying Zeng ◽  
Zhao-Ming Chen ◽  
Wei-Jie Guan ◽  
Tong Wang ◽  
...  
Keyword(s):  
Volatile Organic Compounds ◽  
Organic Compounds ◽  
Single Photon ◽  
Exhaled Breath ◽  
Healthy Controls ◽  
Serum Samples ◽  
Igg Antibody ◽  
Pulmonary Aspergillosis ◽  
Volatile Organic ◽  
Chronic Pulmonary Aspergillosis

Background: Diagnosing chronic pulmonary aspergillosis is a major challenge in clinical practice. The development and validation of a novel, sensitive and specific assay for diagnosing chronic pulmonary aspergillosis is urgently needed.Methods: From April 2018 to June 2019, 53 patients with chronic pulmonary aspergillosis (CPA), 32 patients with community-acquired pneumonia (CAP) and 48 healthy controls were recruited from the First Affiliated Hospital of Guangzhou Medical University. Clinical characteristics and samples were collected at enrollment. All exhaled breath samples were analyzed offline using thermal desorption single-photon ionization time-of-flight mass spectrometry; to analyze the metabolic pathways of the characteristic volatile organic compounds, serum samples were subjected to ultrahigh-performance liquid chromatography.Results: We identified characteristic volatile organic compounds in patients with chronic pulmonary aspergillosis, which mainly consisted of phenol, neopentyl alcohol, toluene, limonene and ethylbenzene. These compounds were assessed using a logistic regression model. The sensitivity and specificity were 95.8 and 96.9% for discriminating patients in the CPA group from those in the CAP group and 95.8 and 97.9% for discriminating patients in the CPA group from healthy controls, respectively. The concentration of limonene (m/z 136) correlated significantly positively with anti-Aspergillus fumigatus IgG antibody titers (r = 0.420, P &lt; 0.01). After antifungal treatment, serum IgG and the concentration of limonene (m/z 136) decreased in the subgroup of patients with chronic pulmonary aspergillosis.Conclusions: We identified VOCs that can be used as biomarkers for differential diagnosis and therapeutic response prediction in patients with chronic pulmonary aspergillosis.

scholarly journals Performance of LDBio Aspergillus WB and ICT Antibody Detection in Chronic Pulmonary Aspergillosis

2021 ◽  
Vol 7 (4) ◽  
pp. 311
Author(s):  
Anna Rozaliyani ◽  
Findra Setianingrum ◽  
Sresta Azahra ◽  
Asriyani Abdullah ◽  
Ayu Eka Fatril ◽  
...  
Keyword(s):  
Receiver Operating Curve ◽  
Antibody Detection ◽  
Pulmonary Aspergillosis ◽  
Comparative Performance ◽  
Tuberculosis Patients ◽  
Chronic Pulmonary Aspergillosis ◽  
Screening Assays ◽  
Roc Area ◽  
Flow Detection ◽  
Sensitivity Specificity

The detection of Aspergillus antibody has a key role in the diagnosis of chronic pulmonary aspergillosis. Western blot (WB) and immunochromatography (ICT) lateral flow detection of Aspergillus antibody can be used as confirmatory and screening assays but their comparative performance in TB patients is not known. This study investigated the performance of these assays among 88 post-tuberculosis patients with suspected CPA. Sensitivity, specificity, receiver operating curve (ROC), area under-curve (AUC) and the agreement between two assays were evaluated. Both WB and ICT showed good sensitivity (80% and 85%, respectively) for detection of Aspergillus antibodies. Substantial agreement (0.716) between these assays was also obtained. The highest AUC result (0.804) was achieved with the combination of WB and ICT. The global intensity of WB correlated with the severity of symptoms in CPA group (p = 0.001). The combination of WB and ICT may increase specificity in CPA diagnosis.

scholarly journals Evaluation of Six Commercial Point-of-Care Tests for Diagnosis of Acute Dengue Infections: the Need for Combining NS1 Antigen and IgM/IgG Antibody Detection To Achieve Acceptable Levels of Accuracy

2011 ◽  
Vol 18 (12) ◽  
pp. 2095-2101 ◽  
Author(s):  
Stuart D. Blacksell ◽  
Richard G. Jarman ◽  
Mark S. Bailey ◽  
Ampai Tanganuchitcharnchai ◽  
Kemajittra Jenjaroen ◽  
...  
Keyword(s):  
Dengue Fever ◽  
Sensitivity And Specificity ◽  
Dengue Infection ◽  
Antibody Test ◽  
Antibody Detection ◽  
Sample Collection ◽  
Igg Antibody ◽  
Igm Antibody ◽  
Ns1 Antigen ◽  
Antibody Tests

ABSTRACTSix assays were evaluated in this study to determine their suitability for the diagnosis of acute dengue infection using samples from 259 Sri Lankan patients with acute fevers (99 confirmed dengue cases and 160 patients with other confirmed acute febrile illnesses): (i) the Merlin dengue fever IgG & IgM combo device (Merlin), (ii) the Standard Diagnostics Dengue Duo nonstructural 1 (NS1) antigen and IgG/IgM combo device (Standard Diagnostics, South Korea), (iii) the Biosynex Immunoquick dengue fever IgG and IgM (Biosynex, France) assay, (iv) the Bio-Rad NS1 antigen strip (Bio-Rad, France), (v) the Panbio Dengue Duo IgG/IgM Cassette (Inverness, Australia), and (vi) the Panbio dengue NS1 antigen strip (Inverness, Australia). The median number of days of fever prior to admission sample collection was 5 days (interquartile range, 3 to 7 days). Sensitivity and specificity of the NS1 antigen tests ranged from 49 to 59% and from 93 to 99%, respectively, and sensitivity and sensitivity of the IgM antibody test ranged from 71 to 80% and from 46 to 90%, respectively. Combining the NS1 antigen and IgM antibody results from the Standard Diagnostics Dengue Duo test gave the best compromise of sensitivity and specificity (93% and 89%, respectively) and provided the best sensitivity in patients presenting at different times after fever onset. The Merlin IgM/IgG antibody tests correctly classified 64% and 86% of the primary and secondary dengue infection cases, respectively, and the Standard Diagnostics IgM/IgG antibody tests correctly classified 71% and 83% of the primary and secondary dengue infection cases, respectively. This study provides strong evidence of the value of combining dengue antigen- and antibody-based test results in the rapid diagnostic test (RDT) format for the acute diagnosis of dengue.

scholarly journals Evaluation of LDBio Aspergillus ICT Lateral Flow Assay for IgG and IgM Antibody Detection in Chronic Pulmonary Aspergillosis

2019 ◽  
Vol 57 (9) ◽  
Author(s):  
Elizabeth Stucky Hunter ◽  
Malcolm D. Richardson ◽  
David W. Denning
Keyword(s):  
United Kingdom ◽  
Line Intensity ◽  
Test Line ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Antibody Detection ◽  
Resource Constrained ◽  
Pulmonary Aspergillosis ◽  
Chronic Pulmonary Aspergillosis ◽  
Specific Igg

ABSTRACT Detecting Aspergillus-specific IgG is critical to diagnosing chronic pulmonary aspergillosis (CPA). Existing assays are often cost- and resource-intensive and not compatible with resource-constrained laboratory settings. LDBio Diagnostics has recently commercialized a lateral flow assay based on immunochromatographic technology (ICT) that detects Aspergillus antibodies (IgG and IgM) in less than 30 min, requiring minimal laboratory equipment. A total of 154 CPA patient sera collected at the National Aspergillosis Centre (Manchester, United Kingdom) and control patient sera from the Peninsula Research Bank (Exeter, United Kingdom) were evaluated. Samples were applied to the LDBio Aspergillus ICT lateral flow assay, and results were read both visually and digitally. Results were compared with Aspergillus IgG titers in CPA patients, measured by ImmunoCAP-specific IgG assays. For proven CPA patients versus controls, sensitivity and specificity for the LDBio Aspergillus ICT were 91.6% and 98.0%, respectively. In contrast, the routinely used ImmunoCAP assay exhibited 80.5% sensitivity for the same cohort (cutoff value, 40 mg of antigen-specific antibodies [mgA]/liter). The assay is easy to perform but challenging to read when only a very faint band is present (5/154 samples tested). The ImmunoCAP Aspergillus IgG titer was also compared with the Aspergillus ICT test line intensity or rate of development, with weak to moderate correlations. The Aspergillus ICT lateral flow assay exhibits excellent sensitivity for serological diagnosis of CPA. Quantifying IgG from test line intensity measurements is not reliable. Given the short run time, simplicity, and limited resources needed, the LDBio Aspergillus ICT is a suitable diagnostic tool for CPA in resource-constrained settings.

scholarly journals Analysis of the relations between allergen specific LgG antibody and allergic dermatosis of 14 kinds foods

Open Medicine ◽  
2015 ◽  
Vol 10 (1) ◽  
Author(s):  
Hu Yin’e ◽  
Dai Shufang ◽  
Wang Bin ◽  
Qu Wei ◽  
Gao Junling ◽  
...  
Keyword(s):  
Food Intolerance ◽  
Antibody Detection ◽  
Igg Antibody ◽  
Allergic Dermatitis ◽  
Positive Antibody ◽  
Significant Difference ◽  
High Incidence ◽  
Positive Rate ◽  
High Incidence Rate ◽  
Specific Igg

AbstractTo use food-specific IgG antibody detection to explore its application in the allergy dermatoses. 181 patients were included from January 2014 to September 2014. Fourteen food-specific IgG antibodies were detected by ELISA. The positive rates of IgG antibody of the patient group and the healthy group were significantly different. The positive rates of IgG antibody of egg, milk, shrimp and crab took a large proportion in three groups of patients with three kinds of allergy dermatoses of urticaria, eczema and allergic dermatitis, the proportion of which was respectively 70.2%, 77.8% and 71.7%. There was mild and moderate intolerance of food in the allergic dermatitis group while there was no distribution difference of food intolerance in urticaria group and eczema group. Among urticaria and allergic dermatitis patients with positive antibody, the positive rate of children was significantly higher than that of adults while there was no significant difference between children and adults among eczema patients with positive antibody. Allergy dermatoses are closely related to food-specific IgG antibody and the allergy dermatoses patients have a high incidence rate of food intolerance; detecting IgG antibody in patients is of great significance for the diagnosis and treatment of allergy dermatoses.

Comparison of three anti-coccidioides antibody enzyme immunoassay kits for the diagnosis of coccidioidomycosis

2020 ◽  
Vol 58 (6) ◽  
pp. 774-778 ◽  
Author(s):  
Joshua Malo ◽  
Eric Holbrook ◽  
Tirdad Zangeneh ◽  
Chris Strawter ◽  
Eyal Oren ◽  
...  
Keyword(s):  
Enzyme Immunoassay ◽  
Diagnostic Testing ◽  
High Risk Patient ◽  
Immunoglobulin M ◽  
Antibody Detection ◽  
Serum Samples ◽  
Igg Antibody ◽  
Igm Antibodies ◽  
Igm Antibody ◽  
Detection Specificity

Abstract Coccidioidomycosis is a common cause of community-acquired pneumonia in endemic areas of the southwestern United States. Clinical presentations range from self-limited disease to severe, disseminated disease. As such, early and accurate diagnosis is essential to ensure appropriate treatment and monitoring. Currently available diagnostic testing has variable accuracy, particularly in certain patient populations, and new tests may offer improved accuracy for the diagnosis of coccidioidomycosis. Serum samples from patients with coccidioidomycosis and controls were tested for immunoglobulin G (IgG) and immunoglobulin M (IgM) antibodies using the MVista Coccidioides antibody detection EIA and two commonly used commercial enzyme immunoassay (EIA) kits: the IMMY Omega EIA and the Meridian Premier EIA. The sensitivity of the IgG antibody detection was 87.4% using the MVista test compared to 46.6% for IMMY and 70.9% for Meridian. The sensitivity for IgM antibody detection was 61.2% for the MVista test, 22.3% for IMMY and 29.1% for Meridian. For IgG antibody detection, specificity was 90% for the MVista EIA, 94.6% for IMMY, 96.4% for Meridian. For IgM antibody detection, specificity was 95.3% for the MVista test 98.2% for IMMY and 99.1% for Meridian. The MVista Coccidioides antibody EIA offers improved sensitivity, including among high-risk patient populations, for the detection of IgG and IgM antibodies in comparison to other currently available EIAs.

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