scholarly journals Effect of Fermented Traditional Chinese Prescription With Lactobacillus Plantarum on Dysbacteriotic Diarrhea Mice Induced by Ceftriaxone Sodium

2021 ◽  
Author(s):  
Xin Guo ◽  
Zipeng Yan ◽  
Jixiang Wang ◽  
Xinfeng Fan ◽  
Jie Kang ◽  
...  
Keyword(s):  
Lactobacillus Plantarum ◽  
Barrier Function ◽  
High Performance ◽  
Intestinal Flora ◽  
Intestinal Barrier ◽  
Intestinal Morphology ◽  
Intestinal Barrier Function ◽  
Flight Mass Spectrometry ◽  
Ceftriaxone Sodium ◽  
Fecal Output

Abstract Background: Buzhongyiqi decoction (B), Sijunzi decoction (S), and Shenlingbaizhu decoction (SH) have been extensively clinically used for the treatment of health status and diseases caused by spleen-qi deficiency for many years and microbial fermentation has been widely applied in Traditional Chinese Medicine (TCM) for thousands of years in China. This study was aimed to investigate the mitigative effect of TCM and fermented TCM (FTCM) with Lactobacillus plantarum (LP) in antibiotic-associated diarrhea (AAD), and identify the compounds of S and Fermented S (FS). Methods: The dysbacteriotic diarrhea mice induced by ceftriaxone sodium (CS) were treated with LP, B, S, SH, Fermented B, S, and SH. The diarrhea indexes, the abundance of some gut bacteria, intestinal morphometrics, and the mRNA expressions related to intestinal barrier function were assessed at multilevels. In addition, S and FS were chosen to identify and relatively quantify the compounds by ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF/MS), and different expressed compounds were analysed. Results: Results showed that CS significantly increased the fecal output weight, the total number of fecal output, and fecal water content, indicating the occurrence of diarrhea, while TCM, LP, and FTCM alleviated the diarrhea to different degrees and FTCM showed more sustained effects. Then, bacterial culture test showed above symptoms were accompanied with the disruption of some intestinal flora. Meanwhile, the diarrhea mice showed abnormal intestinal morphology and destroyed intestinal barrier manifested as reduced mRNA expression of Aquaporins (AQPs) and tight junction (TJ) protein. Notably, the above indices were alleviated in other treatments mice.Conclusions: All these findings imply that the intestinal side effects caused by antibiotics can be alleviated by TCM, LP, and fermented TCM through regulating the intestinal flora and barrier function, which provides an idea of further development and application of them in the clinical use of antibiotics.

Endogenous intoxication syndrome in pyelonephritis and methods of its therapy with the use of nutrient support

2021 ◽  
pp. 40-45
Author(s):  
G. M. Letifov
Keyword(s):  
Barrier Function ◽  
Nutritional Support ◽  
Intestinal Flora ◽  
Intestinal Barrier ◽  
Intestinal Barrier Function ◽  
Antibacterial Prophylaxis ◽  
Urinary System ◽  
Dietary Fibers ◽  
Endogenous Intoxication ◽  
Medical University

Endogenous intoxication syndrome in pyelonephritis and methods of its therapy using nutritional support G.M. Letifov FSBEI HE «Rostov State Medical University Ministry of Health of Russia Rostov-on-Don Summary The aim of the study was to scientifically substantiate and determine the prospects for the use of nutritional support in the form of the use of dietary supplements containing a complex of natural dietary fibers, inulin (from artichoke), effective probiotics-short-chain fructooligosaccharides, maltodextrin 1.5 teaspoon 2 times a day. The course of treatment is 3 weeks. The appointment of a prebiotic complex, prevention of dysbiosis and restoration of the intestinal barrier function significantly reduced the risk of re-entry of opportunistic intestinal flora into the urinary system, thereby reducing the need for antibacterial prophylaxis of recurrent pyelonephritis.

scholarly journals Luhong Granules Prevent Ventricular Remodelling after Myocardial Infarction by Reducing the Metabolites TMAO and LPS of the Intestinal Flora

2019 ◽  
Vol 2019 ◽  
pp. 1-10
Author(s):  
Tianshu Yang ◽  
Huiyan Qu ◽  
Xiaolong Song ◽  
Qian Liu ◽  
Xiaoli Yang ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Barrier Function ◽  
Intestinal Flora ◽  
Intestinal Barrier ◽  
Intestinal Barrier Function ◽  
Sham Operation ◽  
Plasma Samples ◽  
Ventricular Remodelling ◽  
Sham Operation Group ◽  
Operation Group

Background. Ventricular remodelling is a common pathological change at all stages of heart disease. Luhong granules are widely used in patients with chronic ventricular remodelling after myocardial infarction and can alleviate chest tightness, shortness of breath, and other symptoms. However, its effect on ventricular remodelling remains to be studied. Purpose. In this study, we investigated the effects of these granules on myocardial fibrosis in a rat model of myocardial infarction in vivo. Methods. Male Wistar rats were randomly divided into four groups: the sham operation group, the acute myocardial infarction (AMI) group, the Luhong granule group, and the vancomycin group, with a sample size (n) of 10 rats in each group. The AMI model was established in all rats by ligation of the left anterior descending (LAD) coronary artery (the sham operation group did not undergo ligation). Luhong granules (0.5 ml·kg−1·d−1), vancomycin (0.075 g·ml−1·d−1), and 0.9% saline (5 ml·kg−1·d−1 for the sham operation and AMI groups) were administered orally for 6 weeks. Echocardiography was used to check cardiac structure and function. Myocardial and small intestinal tissue morphology was observed by haematoxylin and eosin (H&E) staining, and heart samples were stained with Masson’s trichrome to analyse myocardial fibrosis. 16S rDNA sequencing was performed to detect changes in the gut flora. The level of trimethylamine N-oxide (TMAO) in plasma samples was quantified by stable isotope dilution liquid chromatography-tandem mass spectrometry (LC-MS). Results. H&E and Masson’s trichrome staining of cardiac tissues showed that Luhong granules could partially reverse ventricular remodelling and improve intestinal barrier function (P<0.05). Echocardiographic analysis showed that, compared with the AMI group, the left ventricular ejection fraction (LVEF) in the Luhong granule group was increased (P<0.05). Stool sequencing and microbiological analysis showed changes in Bacteroidales, Alistipes, Phascolarctobacterium, etc., which can produce TMAO. We found that Luhong granules can reduce Bacteroidales, Alistipes, and Phascolarctobacterium at the genus level. The levels of TMAO and lipopolysaccharides (LPS) in plasma samples were reduced in the Luhong granule group (P<0.05). Conclusions. Our results indicate that Luhong granules reduce TMAO and LPS levels in circulating blood by improving intestinal flora and intestinal barrier function to delay ventricular remodelling after myocardial infarction.

Effects of Glutamine on Growth Performance, Intestinal Morphology and Intestinal Barrier Function of Broilers

2020 ◽  
Author(s):  
Q. J. Wu ◽  
Z. H. Liu ◽  
C. Jiao ◽  
B. Y. Cheng ◽  
D. D. Zhu ◽  
...  
Keyword(s):  
Growth Performance ◽  
Feed Intake ◽  
Barrier Function ◽  
Average Daily Gain ◽  
Intestinal Barrier ◽  
Intestinal Morphology ◽  
Intestinal Barrier Function ◽  
Villus Height ◽  
Crypt Depth ◽  
Daily Gain

The effect of glutamine (Gln) on growth performance, intestinal morphology and intestinal barrier function were evaluated in broilers. A total of 320 birds were divided into a control group (CON) and three experimental groups (Gln 1, Gln 2 and Gln 3). Broilers of group CON received basal diet; broilers in group Gln 1, Gln 2 and Gln 3 were supplemented with 0.5%, 1.0% and 1.5 % Gln, respectively, for 42 days. The results indicated that Gln has no influence on the average daily gain (ADG) among the treatments in the periods of 1 to 21 d, 22 to 42 d and 1 to 42 d (P > 0.05). However, Gln improved average daily feed intake (ADFI) and feed intake: average daily gain (F: G), increased the villus height, villus height to crypt depth ratio (V/C) and the activities of sucrose, the ZO1, claudin-1 and occluding mRNA expression levels (P 0.05). Moreover, Gln decreased the crypt depth of jejunum and ileum in broilers at days 21 and 42 (P 0.05). In conclusion, Gln had a positive effect on growth performance and gut parameters by modifying the function of the intestinal mucosa barrier.

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