A Cellular Study of Inflammatory Responses in Women with Polycystic Ovary Syndrome as: “An In vitro Model of Anti Breast Tumor Response”A Case Control Study

Abstract Background: Although Polycystic Ovary syndrome (PCOS) is a common endocrine disorder among women of reproductive age; is unclear whether PCOS increases the risk of subsequent development of, Gynecologic cancers namely breast cancer. The present study we aimed to compare the antitumoral ability of peripheral blood mononuclear cells (PBMCs) of women with PCOS with that of healthy controls using the co-culture system between effector cells and target tumor cell lines. Materials & Methods: PBMCs were isolated from 25 women with PCOS and 25 non hirsute eumenorrheic healthy controls by density gradient centrifugation ficoll. Breast tumor cell lines (MDA-468, MCF-7) were incubated as the two target cells and were cultured adjacent to PBMCs in the transwell co-culture system. Proliferation rate of the effectors cells evaluated by BrdU cell proliferation assay after 48 and 72 hours and T CD3+ lymphocytes were assessed using flow cytometry. TNF-α cytokine production was evaluated in cell culture supernatant by sandwich ELISA technique. Results: After 48 hours incubation with MDA-468 and MCF-7, the mean proliferation score of PBMCs was significantly higher in women with PCOS compared to that of healthy controls (921.04; P=0.021 vs 287.6; P=0.002, respectively). In PCOS women, after 72 hours of incubation, TNF-α concentration was significantly reduced compared to 48-hour cultures (921.04 ± 271.4 pg/dl vs 545.6 ± 151.1 pg/dl at 48 h and 72 h intervals respectively, P<0.05); it was increased in healthy controls. There was no significant difference in CD3+ CD8+ cells between the PCOS group and healthy controls. Conclusion: The ability of PBMCs to produce of TNF-α in women with PCOS decreased gradually; as a result of which they may lack the ability required to form an in vitro efficient antitumor response to breast tumor cell lines. It is assumed that threshold activation of mononuclear cells is reduced in women with PCOS and a low-grade inflammatory condition may provide a positive background for arising myeloid derived suppressor cells (MDSCs).

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Breast Tumor Cell Lines From Pleural Effusions2

JNCI Journal of the National Cancer Institute ◽

10.1093/jnci/53.3.661 ◽

1974 ◽

Vol 53 (3) ◽

pp. 661-674 ◽

Cited By ~ 562

Author(s):

R. Cailleau ◽

R. Young ◽

M. Olivé ◽

W. J. Reeves

Keyword(s):

Chromosome Number ◽

Tumor Cell ◽

Tumor Cells ◽

Cell Lines ◽

Breast Tumor ◽

Tumor Cell Lines ◽

Breast Cancer Patients ◽

Pleural Effusions ◽

Epithelial Tumor

Summary During 1973, 4 new epithelial tumor cell lines were isolated from pleural effusions from breast cancer patients. We describe 3 of these lines: MDA-MB-134, with a mean chromosome number of 43; MDA-MB-175, with a mean chromosome number of 49; and MDA-MB-231, with a mean chromosome number between 65 and 69. We isolated the same cell type from 4 of 10 effusions from MDA-MB-134 and from 6 of 8 effusions from MDA-MB-175. We found that pleural effusions as a source of breast tumor cells to be cultured and studied in vitro have the following advantages: 1) large amounts of material and the possibility of obtaining sequential samples from the same patient; 2) high viability of tumor cells; 3) scarcity or absence of fibroblasts; and 4) the possibility of separating the tumor cells from other “contaminating” cell types by differences in their speed or degree of attachment to the flask. All lines from different patients differed, as seen grossly and microscopically. All lines from sequential pleural effusions from the same patient were apparently alike. No viruses or mycoplasmas were detected in any line.

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Effects of a prenyl-baicalein derivative on ER (+) MCF-7 and ER (−) MDA-MB-231 breast tumor cell lines

Medicinal Chemistry Research ◽

10.1007/s00044-011-9848-1 ◽

2011 ◽

Vol 21 (10) ◽

pp. 3154-3160

Author(s):

Marta Perro Neves ◽

Ana Camões ◽

Maria de São José Nascimento ◽

Honorina Cidade ◽

Maria Emília Sousa ◽

...

Keyword(s):

Tumor Cell ◽

Cell Lines ◽

Breast Tumor ◽

Tumor Cell Lines ◽

Breast Tumor Cell ◽

Mcf 7

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New substituted 4-arylaminoquinazolines as potent inhibitors of breast tumor cell lines: In vitro and docking experiments

European Journal of Medicinal Chemistry ◽

10.1016/j.ejmech.2010.04.034 ◽

2010 ◽

Vol 45 (9) ◽

pp. 4339-4342 ◽

Cited By ~ 7

Author(s):

André N. de Oliveira ◽

Cleverson C. Bocca ◽

João E. Carvalho ◽

Ana Lúcia G. Ruiz ◽

Thiago P. Silva ◽

...

Keyword(s):

Tumor Cell ◽

Cell Lines ◽

Breast Tumor ◽

Tumor Cell Lines ◽

Breast Tumor Cell

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Bioactivities of essential oils from different parts of Spiranthera odoratissima (Rutaceae)

Rodriguésia ◽

10.1590/2175-7860202071050 ◽

2020 ◽

Vol 71 ◽

Author(s):

Fernando Duarte Cabral ◽

Cassia Cristina Fernandes ◽

Arthur Barcelos Ribeiro ◽

Iara Squarisi Squarisi ◽

Denise Crispim Tavares ◽

...

Keyword(s):

Tumor Cell ◽

Cell Lines ◽

Normal Cell ◽

Human Tumor ◽

Tumor Cell Lines ◽

Human Tumor Cell ◽

Human Tumor Cell Lines ◽

Ic50 Values ◽

Mcf 7

Abstract This paper aims to investigate, for the first time, in vitro antitubercular, antileishmanial and antiproliferative activities of essential oils (EOs) from S. odoratissima leaves and flowers - grown in midwestern Brazil - against Mycobacterium tuberculosis, promastigote forms of Leishmania amazonensis and human tumor cell lines. Antimycobacterial activity of EOs was evaluated in terms of the minimal inhibitory concentration (MIC). EOs from leaves and flowers showed to be active antimicrobials against M. tuberculosis, since MIC values were 150 µg/mL and 162.5 µg/mL, respectively. Both EOs exhibited significant activity against promastigote forms of L. amazonensis; IC50 values (50% growth inhibition) were 14.36 ± 2.02 (EOs from leaves) and 19.89 ± 2.66 µg/mL (EOs from flowers). Antiproliferative activity in normal (GM07492A, lung fibroblasts) and tumor (MCF-7, HeLa and M059J) cell lines was performed by the XTT assay; results were expressed as IC50 (50% cell growth inhibition) and the selective index was calculated. IC50 values of EOs from leaves and flowers obtained in normal cell lines for were 502.97 ± 40.33 µg/mL and 370.60 ± 2.01 µg/mL, respectively. Antiproliferative activity was observed against human tumor cell lines, whose IC50 values were significantly lower than those obtained in normal cell lines of MCF-7 cells (367.57 ± 4.46 µg/mL-EOs from leaves and 357.70 ± 1.85 µg/mL-EOs from flowers) and M059J cells (492.53 ± 56.67 µg/mL-EOs from leaves and 324.90 ± 6.72 µg/mL-EOs from flowers), thus, indicating selectivity. These in vitro results showed that EOs from S. odoratissima may be an antimycobacterial, antiparasitic and antitumor agent.

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The Multidrug Resistance of In Vitro Tumor Cell Lines Derived from Human Breast Carcinoma MCF-7 Does Not Influence Pentavalent Technetium-99m-Dimercaptosuccinic Acid Uptake

Cancer Biotherapy & Radiopharmaceuticals ◽

10.1089/108497803770418337 ◽

2003 ◽

Vol 18 (5) ◽

pp. 791-801 ◽

Cited By ~ 4

Author(s):

Delphine Denoyer ◽

Nathalie Perek ◽

Nathalie Le Jeune ◽

Delphine Frère ◽

Francis Dubois

Keyword(s):

Multidrug Resistance ◽

Breast Carcinoma ◽

Tumor Cell ◽

Cell Lines ◽

Acid Uptake ◽

Tumor Cell Lines ◽

Human Breast ◽

Technetium 99M Dimercaptosuccinic Acid ◽

Mcf 7

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Synthesis and Antiplasmodial Activity of 1,2,3-Triazole-Naphthoquinone Conjugates

Molecules ◽

10.3390/molecules24213917 ◽

2019 ◽

Vol 24 (21) ◽

pp. 3917 ◽

Cited By ~ 1

Author(s):

Oramas-Royo ◽

López-Rojas ◽

Amesty ◽

Gutiérrez ◽

Flores ◽

...

Keyword(s):

Plasmodium Falciparum ◽

Tumor Cell ◽

Cell Lines ◽

Antiproliferative Activity ◽

Antimalarial Activity ◽

Tumor Cell Lines ◽

Dihydroorotate Dehydrogenase ◽

Structure Activity ◽

Mcf 7

A series of 34 1,2,3-triazole-naphthoquinone conjugates were synthesized via copper-catalyzed cycloaddition (CuAAC). They were evaluated for their in vitro antimalarial activity against chloroquine-sensitive strains of Plasmodium falciparum and against three different tumor cell lines (SKBr-3, MCF-7, HEL). The most active antimalarial compounds showed a low antiproliferative activity. Simplified analogues were also obtained and some structure–activity relationships were outlined. The best activity was obtained by compounds 3s and 3j, having IC50 of 0.8 and 1.2 μM, respectively. Molecular dockings were also carried on Plasmodium falciparum enzyme dihydroorotate dehydrogenase (PfDHODH) in order to rationalize the results.

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Lymphokine-Activated Killer Cytotoxicity in Neonatal Mononuclear Cells: In Vitro Responses to Tumor Cell Lines from Pediatric Solid Tumors1

Pediatric Research ◽

10.1203/00006450-198902000-00016 ◽

1989 ◽

Vol 25 (2) ◽

pp. 156-160 ◽

Cited By ~ 16

Author(s):

Terry Chin ◽

Cindee Toy ◽

Carmella Vandeven ◽

Mitchell S Cairo

Keyword(s):

Tumor Cell ◽

Cell Lines ◽

Mononuclear Cells ◽

Tumor Cell Lines

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The Effect of Cucurbit[7]uril on the Antitumor and Immunomodulating Properties of Oxaliplatin and Carboplatin

International Journal of Molecular Sciences ◽

10.3390/ijms22147337 ◽

2021 ◽

Vol 22 (14) ◽

pp. 7337

Author(s):

Ekaterina Pashkina ◽

Alina Aktanova ◽

Irina Mirzaeva ◽

Ekaterina Kovalenko ◽

Irina Andrienko ◽

...

Keyword(s):

Tumor Cell ◽

Cell Lines ◽

Mononuclear Cells ◽

Biological Properties ◽

In Vivo Studies ◽

Tumor Cell Lines ◽

Peripheral Blood Mononuclear ◽

The Impact

Cucurbit[7]uril (CB[7]) is a molecular container that may form host–guest complexes with platinum(II) anticancer drugs and modulate their efficacy and safety. In this paper, we report our studies of the effect of CB[7]–oxaliplatin complex and the mixture of CB[7] and carboplatin (1:1) on viability and proliferation of a primary cell culture (peripheral blood mononuclear cells), two tumor cell lines (B16 and K562) and their activity in the animal model of melanoma. At the same time, we studied the impact of platinum (II) drugs with CB[7] on T cells and B cells in vitro. Although the stable CB[7]–carboplatin complex was not formed, the presence of cucurbit[7]uril affected the biological properties of carboplatin. In vivo, CB[7] increased the antitumor effect of carboplatin, but, at the same time, increased its acute toxicity. Compared to free oxaliplatin, its complex with CB[7] shows a greater cytotoxic effect on tumor cell lines B16 and K562, while in vivo, the effects of the free drug and encapsulated drug were comparable. However, in vivo studies also demonstrated that the encapsulation of oxaliplatin in CB[7] lowered the toxicity of the drug.

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