scholarly journals Bioinformatics Analyses of Serine Acetyltransferase (SAT) Gene Family in Rice (Oryza sativa) and their Expressions under Salt Stress

2021 ◽  
Author(s):  
Fırat Kurt ◽  
Ertugrul Filiz ◽  
Adnan Aydın
Keyword(s):  
Oryza Sativa ◽  
Salt Stress ◽  
Active Sites ◽  
Functional Divergence ◽  
Purifying Selection ◽  
Optimal Growth ◽  
Serine Acetyltransferase ◽  
Rice Varieties ◽  
Bioinformatics Analyses ◽  
Wet Lab

Abstract Assimilation of sulfur to cysteine occurs in the presence of serine acetyltransferase (SAT). In this study, SAT genes in rice (Oryza sativa) were identified and analyzed using bioinformatics approaches. Also, these genes were tested under salt stress. OsSATs have two common motifs, bacterial transferase hexapeptide and acetyltransferase and underwent purifying selection. They have more similar protein sequences compared to Arabidopsis. However, there is structural and functional divergence among OsSATs which may be driven by the segmental and tandem duplications. Purifying selection and gene duplications may also have effect leading to variation of specificity and selectivity of OsSATs. In this regard, Asp (D), His (H), Gly (G), Thr (T), Arg (R), Ala (A), and Leu (L) are identified as well-conserved residues in their active sites which have an indicator role on their functions. The OsSATs expressions in different tissues, organs and under hormones showed that jasmonic acid was main hormone inducing the expressions of OsSAT1;1, OsSAT2;1, and OsSAT2;2 whereas auxin and abscisic acid only triggered OsSAT1;1 expression. On the other hand, wet-lab expressions of OsSATs in this study indicated that OsSAT1;1, OsSAT1;2 and OsSAT1;3 genes were upregulated under different exposure times of salt stress. OsSAT1;1 is the only OsSAT induced by various situmuli. The findings can be used by plant breeders and genetic engineers to develop new rice varieties having optimal growth and stress tolerance.

Combination of traits at two developmental stages under salt stress as a measure of tolerance in a reciprocally crossed rice (Oryza sativa) population

2020 ◽  
Vol 71 (4) ◽  
pp. 334 ◽  
Author(s):  
Sabrina M. Elias ◽  
M. Sazzadur Rahman ◽  
Sumaiya F. Khan ◽  
Sudip Biswas ◽  
Taslima Haque ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Salt Stress ◽  
Reciprocal Cross ◽  
Developmental Stages ◽  
Oryza Sativa L ◽  
Principal Component ◽  
Maturity Stage ◽  
Salt Tolerant ◽  
Rice Varieties ◽  
Breeding Lines

The rice (Oryza sativa L.) landrace Horkuch from Bangladesh maintains efficient photosynthesis and detoxification under salt stress and was therefore considered to be a useful donor for tolerance traits. Reciprocally crossed bi-parental mapping populations were generated from salt-tolerant Horkuch and high-yielding salt-sensitive variety IR29, in order to identify superior salt-tolerant high-yielding lines as donors. The present study reports on the phenotypic screening data of ~300 F3 segregating populations from the reciprocal cross and their parental lines in seedlings and screening of a subset at maturity stage under gradual salt stress of 12 dS m–1 for seedlings and 8 dS m–1 for mature plants. Correlation, broad-sense heritability and principal component analyses for salt tolerance as well as yield-related traits were conducted in the populations at the two developmental stages. Level of salt injury was found to be correlated with traits such as filled grain weight at maturity stage and biomass-related traits at the seedling stage. This association between yield-related and survival traits helped to identify tolerant and sensitive plants, which were predicative of agronomic performance under salt stress. Moreover, use of the reciprocal-cross population showed how cytoplasmic inheritance of specific traits such as K+ concentrations can affect characteristics of donor plants. Measurement of a large number of traits and analysis of their co-inherited interrelation can therefore help identify the best performing plants under salt stress for effective breeding strategies. The data are being utilised in mapping of quantitative trait loci, and selected progenies are being used as breeding lines for producing durable salt-tolerant, high-yielding rice varieties.

scholarly journals Determination of germination and seedling growth parameters of rice (Oryza sativa L.) varieties under stress conditions

2020 ◽  
Vol 12 (3) ◽  
pp. 693-701
Author(s):  
Fatih ÖNER ◽  
Ayşe Ö. Ş. SOYSAL
Keyword(s):  
Oryza Sativa ◽  
Salt Stress ◽  
Growth Parameters ◽  
Oryza Sativa L ◽  
Germination Rate ◽  
Dry Weight ◽  
Germination Percentage ◽  
Abiotic Factor ◽  
Rice Varieties ◽  
Stress Levels

Rice (Oryza sativa L.) is an important food for more than half of the world’s population. Globally, rice is grown on approximately 160 million hectares with an average annual production of 740.96 million tonnes. Salinity is an important abiotic factor for germination. In this study rice varieties were analysed for salt stress tolerance at germination growth stage. The response of eight rice varieties against six (0, 25, 50, 75, 100, 150 mM NaCl) salinity levels were studied at germination stage. Seeds of eight rice varieties (‘Yatkin’, ‘Miss-2013 Passali’, ‘Kale’, ‘Manyas Yildizi’, ‘Efe’, ‘Biga Incisi’ and ‘Osmancik-97’) were kept under six salt stress levels. Number of seeds germinated (number), germination rate (%), average germination time, fresh and dry weight of germinated seeds (g), fresh and dry weight of the coleoptile (g), fresh and dry weight of the radicle (g), the length of the coleoptile and radicle (mm) were recorded. The results showed that with increasing salt stress, germination in all the varieties was delayed. The increase in salt stress also reduced every measured trait significantly in all the varieties. Maximum germination percentage (100%) was observed in ‘Biga Incisi’ under all the salt stress levels. Our research data would be helpful for identification of the tolerant varieties which can be studied further in terms of economically.

Systematic Annotation and Bioinformatics Analyses of Large-Scale Oryza sativa Proteome

2011 ◽  
Vol 12 (7) ◽  
pp. 621-630 ◽  
Author(s):  
Lili Liu ◽  
Lin Bai ◽  
Cong Luo ◽  
Donglin Huang ◽  
Ming Chen
Keyword(s):  
Oryza Sativa ◽  
Large Scale ◽  
Bioinformatics Analyses

High-density linkage mapping for agronomic and physiological traits of rice (Oryza sativa L.) under reproductive-stage salt stress

2021 ◽  
Vol 100 (2) ◽  
Author(s):  
Mostafa Ahmadizadeh ◽  
Nadali Babaeian-Jelodar ◽  
Ghasem Mohammadi-Nejad ◽  
Nadali Bagheri ◽  
Rakesh Kumar Singh
Keyword(s):  
Oryza Sativa ◽  
Salt Stress ◽  
Linkage Mapping ◽  
Oryza Sativa L ◽  
High Density ◽  
Reproductive Stage ◽  
Physiological Traits

Silicon alleviates salt stress-induced potassium deficiency by promoting potassium uptake and translocation in rice (Oryza sativa L.)

2021 ◽  
Vol 258-259 ◽  
pp. 153379
Author(s):  
Guochao Yan ◽  
Xiaoping Fan ◽  
Wanning Zheng ◽  
Zixiang Gao ◽  
Chang Yin ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Salt Stress ◽  
Oryza Sativa L ◽  
Potassium Deficiency ◽  
Potassium Uptake

Differential responses of two rice varieties to salt stress

2010 ◽  
Vol 5 (1) ◽  
pp. 89-103 ◽  
Author(s):  
N. Ghosh ◽  
M. K. Adak ◽  
P. D. Ghosh ◽  
S. Gupta ◽  
D. N. Sen Gupta ◽  
...  
Keyword(s):  
Salt Stress ◽  
Rice Varieties ◽  
Differential Responses
Sign in / Sign up

Export Citation Format

Share Document