scholarly journals In Vitro Study of the Effect of HBV Infection on Early Embryonic Development

2021 ◽  
Author(s):  
Feng Ye ◽  
Zixin Cui ◽  
Ying Kong ◽  
Lei Shi ◽  
Jianzhou Li ◽  
...  
Keyword(s):  
Viral Load ◽  
High Load ◽  
Hbv Infection ◽  
Culture Solution ◽  
Infection Group ◽  
Vitro Fertilization ◽  
Low Load ◽  
Embryo Cells ◽  
Infertile Couples

Abstract Objective: This study aimed to clarify the infection state of early embryos by HBV and the effect of HBV infection on embryo development. Methods: In vitro culture was carried out on the fresh surplus embryos from infertile couples on the third day (D3) who received routine in vitro fertilization and embryo transfer (IVF-ET) treatment. The embryo cells were infected in vitro by the serum of HBV infected patients with different viral loads. HBsAg quantity in the supernatant secreted by embryo and HBV mRNA in embryo cells were detected. The development of embryo cells after HBV infection was evaluated by embryo morphological parameter score and fragment score in embryo cells. Results: From May 2013 to July 2015, 238 cases of D3 fresh surplus embryos from 120 infertile couples and 128 samples of embryo culture supernatant were collected. The HBsAg positive rate in the supernatant of embryos in the high viral load infection group was significantly higher than that in the low viral load infection group (40.0% vs.15.6%, p<0.05). HBV mRNA was detected in 2 of 17 HBsAg positive embryos. The scores of embryos in the high load HBV culture solution group were higher than those in the low load group (p=0.04). There was one formed blastocyst in low load HBV culture group, but there was no blastocyst formation in all embryo cells of high load group (p<0.001).Conclusion: The results suggest that embryo cells can be directly infected by HBV in vitro. HBV infection affects the development of embryos.

scholarly journals Effect of Nursing Guidelines on Coping of Infertile Couples Undergoing In Vitro Fertilization

2020 ◽  
Vol 2 (2) ◽  
pp. 11
Author(s):  
Gehan S. Abdelgelel ◽  
Shadia H. Muhsib ◽  
Mona H. Abdelaal ◽  
Randa M. Ibrahim
Keyword(s):  
In Vitro Fertilization ◽  
Coping Strategies ◽  
Unprotected Sex ◽  
The Body ◽  
University Hospital ◽  
Vitro Fertilization ◽  
Significant Difference ◽  
Infertile Couples ◽  
Nursing Guidelines

Context: Infertility is defined as not being able to conceive after one year of unprotected sex. In vitro fertilization (IVF) is a process of fertilization where an ovum is combined with sperm outside the body, in vitro. In vitro fertilization (IVF) is psychologically and emotionally stressful. Coping strategies are needed to master, tolerate, reduce, or minimize stressful events. Aim: This study aimed to evaluate the effect of nursing guidelines on coping of infertile couples' undergoing In Vitro Fertilization. Methods: A quasi-experimental research design was utilized to achieve the aim of this study. This study conducted at the assisted reproductive technology unit of Ain shams Maternity University Hospital on a convenient sample of 98 couples undergoing fertility treatments. Two tools were used for data collection; the first tool was a structured interviewing questionnaire to assess the couple's socio-demographic data, obstetric history, the couple's knowledge regarding in vitro fertilization. The second tool was ways of coping scale (WQS) to assess coping strategies among the infertile couple. Results: There is no statistically significant difference between couples in both groups according to their knowledge and their coping strategies to IVF before the implementation of nursing guidelines (p> 0.05). In contrast, there is a highly statistically significant improvement in knowledge and coping strategies of couples on the study group compared to control group couples after implementation of nursing guidelines(p<0.001). Conclusion: The finding of the current study supported the hypothesis, which stated that the infertile couples who will expose to the nursing guidelines, will exhibit improved coping strategies to IVF compared to the controls. The study recommended the application of nursing guidelines at the IVF unit of Ain Shams Maternity University Hospital and other settings for IVF treatment as routine care to improve infertile couples' coping strategies.

Gene Expression Analysis on the Early Development of Pig Embryos Exposed to Malathion

2007 ◽  
Vol 26 (2) ◽  
pp. 143-149 ◽  
Author(s):  
Zayil Salazar ◽  
Yvonne Ducolomb ◽  
Miguel Betancourt ◽  
Edmundo Bonilla ◽  
Leticia Cortés ◽  
...  
Keyword(s):  
Gene Expression ◽  
Molecular Mechanisms ◽  
Target Genes ◽  
Hypothetical Protein ◽  
Cdna Libraries ◽  
Mhc I ◽  
Vitro Fertilization ◽  
Embryo Cells ◽  
Gene Expression Alterations

Malathion is a widely used pesticide and there is evidence that it could alter mammal’s germ and somatic cells, as well as cell lines. There are not enough studies showing how the nonacute malathion doses affect gene expression. This study analyzes gene expression alterations in pig morular embryos exposed in vitro , for 96 h, to several malathion concentrations after in vitro fertilization. cDNA libraries of isolated morular embryos were created and differential screenings performed to identify target genes. Seven clones were certainly identified. Genes related to mitochondrial metabolism as cytochrome c subunits I and III, nuclear genes such as major histocompatibility complex I (MHC I), and a hypothetical protein related with a splicing factor were the target of malathion’s deregulation effect. The widespread use of malathion as a pesticide should be regarded with reproductive implications and more detailed analysis would yield more about molecular mechanisms of malathion injury on embryo cells.

scholarly journals Epididimis: van hoofspeler tot bysaak?

1997 ◽  
Vol 16 (1) ◽  
pp. 10-15
Author(s):  
M. H. Fourie ◽  
M. S. Bornman ◽  
J. M. C. Oosthuizen
Keyword(s):  
In Vitro Fertilization ◽  
Vitro Fertilization ◽  
Progressive Motility ◽  
Injection Techniques ◽  
Infertile Couples

During epididymal transit, sperm acquire the capacity for fertilization and progressive motility. The epididymis is functionally and mechanically indispensable for in vivo fertilization, but technology has progressed rapidly in the field of in vitro fertilization. Modern sperm-injection techniques have dispensed with the epididymis as a necessity in the treatment of individual infertile couples.

scholarly journals Clinical Outcomes of In Vitro Fertilization among Chinese Infertile Couples Treated for Syphilis Infection

PLoS ONE ◽  
2015 ◽  
Vol 10 (7) ◽  
pp. e0133726 ◽  
Author(s):  
Jing Wang ◽  
Xiaomiao Zhao ◽  
Ping Yuan ◽  
Tingfeng Fang ◽  
Nengyong Ouyang ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Clinical Outcomes ◽  
Syphilis Infection ◽  
Vitro Fertilization ◽  
Infertile Couples

scholarly journals Sterility, an Overlooked Health Condition

Women ◽  
2020 ◽  
Vol 1 (1) ◽  
pp. 29-45
Author(s):  
Rachel Royfman ◽  
Tariq A. Shah ◽  
Puneet Sindhwani ◽  
Nagalakshmi Nadiminty ◽  
Tomer Avidor-Reiss
Keyword(s):  
In Vitro Fertilization ◽  
Meta Analysis ◽  
Health Condition ◽  
Vitro Fertilization ◽  
Biological Child ◽  
Live Births ◽  
Clinical Literature ◽  
Primary Literature ◽  
Infertile Couples

Clinically, infertility is defined as the inability to conceive after a certain period. In contrast, sterility is defined as the inability to produce a biological child; however, this is not a practical definition that can be applied in a clinical setting to a patient’s diagnosis. Unlike infertility, sterility is rarely discussed in biomedical and clinical literature and is often used synonymously with infertility. Infertility affects about 10% of couples globally, but the prevalence of sterility remains unknown. We divide sterility into three subtypes natural, clinical, and hardship. To estimate sterility prevalence, we analyzed primary literature and meta-analysis papers on the rates of live births and pregnancies throughout several treatments of infertile couples (e.g., untreated patients, in vitro fertilization-treated, and patients administered other treatments). This analysis indicates that all treatments fail in delivering a biological child to most couples, suggesting that most infertile couples may fail to conceive. More comprehensive primary studies are needed to provide a precise estimate of sterility. Furthermore, research is needed to study the causes of sterility, as well as develop methods for diagnosis and treatment that are financially affordable and emotionally tolerable. Altogether, sterility is an under-discussed condition that is more common than expected, as many infertile couples are unable to conceive and are, in effect, sterile.

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