scholarly journals Adjuvanticity of sliver nanoparticles with Infectious bursal disease virus VP2 protein and its effect on humoral immune response in chickens

2021 ◽  
Author(s):  
Sudhakar P. Awandkar ◽  
Prabhakar A Tembhurne ◽  
Nitin V Kurkure ◽  
Sandeep P Chaudhari ◽  
Sachin W Bonde ◽  
...  
Keyword(s):  
Immune Response ◽  
Silver Nanoparticles ◽  
Disease Virus ◽  
Humoral Immune Response ◽  
Infectious Bursal Disease Virus ◽  
Gene Segment ◽  
Infectious Bursal Disease ◽  
Vp2 Protein ◽  
Humoral Immune ◽  
Bursal Disease

Abstract The present study was conducted to decipher the effect of silver nanoparticles conjugated marker recombinant VP2 immunogen of Infectious bursal disease virus on humoral immune response in chickens. The hypervariable VP2 gene segment of field Infectious bursal disease virus, consisting of major and minor hydrophilic loops, was amplified using reverse transcription-polymerase chain reaction. The gene segment of size 664 bp was cloned into pGEM-T Easy plasmid followed by subcloning into pET32a plasmid vector. Truncated recombinant VP2 protein (rVP2, 19 kDa) was expressed in a prokaryotic expression system using Escherichia coli BL32DE3 cells. The rVP2 protein showed reactivity with specific anti VP2 chicken antibodies. The results of Western blot revealed its utility in serological diagnosis. The recombinant antigen was tested for immunogenic potential by vaccinating the chickens with and without silver nanoparticles. The rVP2 protein blended with adjuvant grade montanide oil showed a highly significant rise in serum IgY titers. The titers induced by rVP2 protein mixed with montanide oil were non-significant when compared with titers induced by the conventional vaccines. The IgY response was highly significant in chickens vaccinated with rVP2 protein blended with montanide oil and silver nanoparticles than in chickens vaccinated with conventional vaccines or rVP2 protein. The results represent Infectious bursal disease virus rVP2 protein as a promising candidate for the DIVA vaccine and sero-diagnostic tool. For the first time, the current study elucidated the adjuvanticity effect of silver nanoparticles on avian Infectious bursal disease virus rVP2 vaccine potency.

scholarly journals Comparative Efficacy and Humoral Responses of an Inactivated Infectious Bursal Disease Virus Vaccine Prepared from a Local Isolate with that of a Commercial Live Vaccine in Layer Birds

1970 ◽  
Vol 2 (1) ◽  
pp. 37-38
Author(s):  
MIA Begum ◽  
MA Islam ◽  
ATM Mahbub-E-Elahi ◽  
M Rahman ◽  
MER Bhuiyan
Keyword(s):  
Immune Response ◽  
Disease Virus ◽  
Humoral Immune Response ◽  
Infectious Bursal Disease Virus ◽  
Live Vaccine ◽  
Infectious Bursal Disease ◽  
Inactivated Vaccine ◽  
Humoral Immune ◽  
Local Isolate ◽  
Bursal Disease

The humoral immune response and efficacy of an inactivated adjuvanted infectious bursal disease virus (IBDV) vaccine prepared with a virulent local isolate was compared with a live commercial IBDV vaccine (Nobilis D78®, Intervet) in 20 layer birds during the period from October to November 2002. These day-old experimental birds were divided into four groups, A, B, C and D, each consisting of 5 birds. Each bird of groups A, B and C was immunized with live IBDV vaccine (Nobilis D78®, Intervet), live + inactivated vaccine, and inactivated IBDV vaccine, respectively at day 7, day 21 and day 28, whereas birds of group D served as unvaccinated controls. The sera of chickens vaccinated with either combined (live + inactivated) or only inactivated IBDV vaccine showed clear band of precipitation with agar gel immunodiffusion test (AGIDT) and higher antibody titre with ELISA. The protection test revealed that the experimentally prepared inactivated IBDV vaccine gave 100% protection against 80% protection in layer birds immunized with live commercial vaccine. Key words: Efficacy; humoral immune response; IBD: inactivated vaccine; live vaccine; layer birds doi: 10.3329/bjvm.v2i1.1932 Bangl. J. Vet. Med. (2004). 2 (1) : 37-38

Effective protection by high efficiency bicistronic DNA vaccine against infectious bursal disease virus expressing VP2 protein and chicken IL-2

Vaccine ◽  
2009 ◽  
Vol 27 (6) ◽  
pp. 864-869 ◽  
Author(s):  
Sachin Kumar ◽  
Yadvinder S. Ahi ◽  
Shardul S. Salunkhe ◽  
Monika Koul ◽  
Ashok K. Tiwari ◽  
...  
Keyword(s):  
Disease Virus ◽  
Dna Vaccine ◽  
Infectious Bursal Disease Virus ◽  
High Efficiency ◽  
Infectious Bursal Disease ◽  
Effective Protection ◽  
Vp2 Protein ◽  
Bursal Disease

scholarly journals A Comparative Study of Pathology and Host Immune Response Induced by Very Virulent Infectious Bursal Disease Virus in Experimentally Infected Chickens of Aseel and White Leghorn Breeds

Vaccines ◽  
2020 ◽  
Vol 8 (4) ◽  
pp. 627
Author(s):  
Shyama N. Prabhu ◽  
Ajay Pratap Singh ◽  
Berin P. Varghese ◽  
Kuldeep Dhama ◽  
Shambhu Dayal Singh ◽  
...  
Keyword(s):  
Gene Expression ◽  
Immune Response ◽  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Innate Immune ◽  
Infectious Bursal Disease ◽  
White Leghorn ◽  
Viral Loads ◽  
White Leghorns ◽  
Bursal Disease

Indigenous breeds of young chickens in India are believed to be resistant to the classical strain of infectious bursal disease virus (IBDV). However, the mechanism underlying this resistance is obscure. Innate immunity is a key factor in defining the clinical course and pathology of microbial infections. The present study is aimed to compare the pathology of very virulent IBDV (vvIBDV) and immunological host response in experimentally infected - vaccinated and unvaccinated indigenous Aseel and commercial White Leghorn chickens. The viral loads and innate immune gene expression profiles of MDA-5, Mx, IFN-α, and IFN-β in different lymphoid organs were analyzed by quantitative PCR. The histopathological scores in Aseel birds were lower than in White Leghorns despite comparable viral loads. The degrees of histopathological lesions were fewer in vaccinated birds than in unvaccinated birds of both breeds. Analysis of innate immune response genes revealed that the cytoplasmic pattern recognition receptor MDA-5 gene was overexpressed mainly in the cecal tonsils of both vaccinated and nonvaccinated White Leghorn chickens. An increase in the expression of the IFN-α gene was seen in the cecal tonsils of Aseels, and an increase in IFN-β gene expression was seen in the thymuses of White Leghorns following vvIBDV challenge both in vaccinated and nonvaccinated birds. In addition, we observed that the Mx gene plays a minimal role, if any, in vvIBDV infection of the breeds under study. It remains interesting and important that although vvIBDV causes disease in indigenous Aseel birds, the faster clearance and reduced pathology of the virus in Aseel birds compared to White Leghorn chicken indicate some unidentified innate immune factors that are limiting IBDV in this breed. Further studies will be required to correlate kinetics of humoral and cellular immune response in relation to the virus load in different organs to illuminate the mechanism of genetic resistance in native breeds of chicken.

Sign in / Sign up

Export Citation Format

Share Document