scholarly journals Expanding Structural Diversity In A Library Of Disulfide Macrocycles Through In-Situ Imide Hydrolysis

2021 ◽  
Author(s):  
Marcin Konopka ◽  
Artur R. Stefankiewicz
Keyword(s):  
Structural Diversity ◽  
Dynamic Covalent Chemistry ◽  
Dmso Concentration ◽  
Hydrolysis Of

Abstract We describe here an unorthodox approach to dynamic covalent chemistry in which the initially-unexpected in-situ hydrolysis of a bis-imide component is used to control the composition of a library of structurally diverse macrocycles. The dual-stimuli method is based on simultaneous changes in pH and DMSO concentration to expand the diversity of the macrocyclic architectures.

scholarly journals Expanding structural diversity in a library of disulfide macrocycles through in-situ imide hydrolysis

2022 ◽  
Vol 12 (1) ◽  
Author(s):  
Marcin Konopka ◽  
Artur R. Stefankiewicz
Keyword(s):  
Phase Transfer ◽  
Structural Diversity ◽  
Single Step ◽  
Water Soluble ◽  
One Pot ◽  
Dynamic Covalent Chemistry ◽  
Complex Process ◽  
Dmso Concentration ◽  
Hydrolysis Of

AbstractWe describe here an unorthodox approach to dynamic covalent chemistry in which the initially-unexpected in-situ hydrolysis of a bis-imide is employed to control the composition of a library of structurally diverse macrocycles. A single building block is used to generate a library of numerous disulfide-based architectures in a one-pot single-step process. The dual-stimuli method is based on simultaneous changes in pH and DMSO concentration to expand the structural diversity of the macrocyclic products. Mechanistic details of this complex process are investigated by the kinetics analysis. We delivered a facile strategy for the synthesis of water-soluble, multicomponent and dynamic macrocycles equipped with number of different functional groups, thus giving a prospect of their application in guest-driven phase transfer.

INDIREKTE PAPIERCHROMATOGRAPHISCHE METHODE ZUR BESTIMMUNG DER HARNOESTROGENE

1961 ◽  
Vol 38 (4) ◽  
pp. 545-562 ◽  
Author(s):  
L. Kecskés ◽  
F. Mutschler ◽  
I. Glós ◽  
E. Thán ◽  
I. Farkas ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Granulosa Cell ◽  
Iron Content ◽  
List Type ◽  
Granulosa Cell Tumour ◽  
Hydrolysis Of ◽  
Phenol Fraction ◽  
Qualitative Determination

ABSTRACT 1. An indirect paperchromatographic method is described for separating urinary oestrogens; this consists of the following steps: acidic hydrolysis, extraction with ether, dissociation of phenol-fractions with partition between the solvents. Previous purification of phenol fraction with the aid of paperchromatography. The elution of oestrogen containing fractions is followed by acetylation. Oestrogen acetate is isolated by re-chromatography. The chromatogram was developed after hydrolysis of the oestrogens 'in situ' on the paper. The quantity of oestrogens was determined indirectly, by means of an iron-reaction, after the elution of the iron content of the oestrogen spot, which was developed by the Jellinek-reaction. 2. The method described above is satisfactory for determining urinary oestrogen, 17β-oestradiol and oestriol, but could include 16-epioestriol and other oestrogenic metabolites. 3. The sensitivity of the method is 1.3–1.6 μg/24 hours. 4. The quantitative and qualitative determination of urinary oestrogens with the above mentioned method was performed in 50 pregnant and 9 non pregnant women, and also in 2 patients with granulosa cell tumour.

In Situ Microscopy for In-line Monitoring of the Enzymatic Hydrolysis of Cellulose

2013 ◽  
Vol 85 (17) ◽  
pp. 8121-8126 ◽  
Author(s):  
Britta Opitz ◽  
Andreas Prediger ◽  
Christian Lüder ◽  
Marrit Eckstein ◽  
Lutz Hilterhaus ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Enzymatic Hydrolysis Of Cellulose ◽  
Hydrolysis Of Cellulose ◽  
Hydrolysis Of

scholarly journals Permeability of muscle capillaries to small heme-peptides. Evidence for the existence of patent transendothelial channels.

1975 ◽  
Vol 64 (3) ◽  
pp. 586-607 ◽  
Author(s):  
N Simionescu ◽  
M Siminoescu ◽  
G E Palade
Keyword(s):  
Plasma Proteins ◽  
Intercellular Junctions ◽  
Rat Diaphragm ◽  
Enzymic Hydrolysis ◽  
Graphic Analysis ◽  
Heme Peptides ◽  
Future Work ◽  
Hydrolysis Of ◽  
Muscle Capillaries

Two heme-peptides (HP) of about 20-A diameter (heme-undecapeptide [H11P], mol wt approximately 1900 and heme-octapeptide [H8P], mol wt approximately 1550), obtained by enzymic hydrolysis of cytochrome c, were sued as probe molecules in muscle capillaries (rat diaphragm). They were localized in situ by a perixidase reaction, enhanced by the addition of imidazole to the incubation medium. Chromatography of plasma samples showed that HPs circulate predominantly as monomers for the duration of the experiments and are bound by aldehyde fixatives to plasma proteins to the extent of approximately 50% (H8P) to approximately 95% (H11P). Both tracers cross the endothelium primarily via plasmalemmal vesicles which become progressively labeled (by reaction product) from the blood front to the tissue front of the endothelium, in three successive resolvable phases. By the end of each phase the extent of labeling reaches greater than 90% of the corresponding vesicle population. Labeled vesicles appear as either isolated units or chains which form patent channels across the endothelium. The patency of these channels was checked by specimen tilting and graphic analysis of their images. No evidence was found for early or preferential marking of the intercellular junctions and spaces by reaction product. It is concluded that the channels are the most likely candidate for structural equivalents of the small pores of the capillary wall since they are continuous, water-filled passages, and are provided with one or more strictures of less than 100 A. Their frequency remains to be established by future work.

Enzymatic Hydrolysis of PET: Structural Diversity and Kinetic Properties of Cutinases from Thermobifida

2014 ◽  
Vol 31 ◽  
pp. S88
Author(s):  
Altijana Hromic ◽  
Doris Ribitsch ◽  
Andrzej Lyskowski ◽  
Georg Steinkellner ◽  
Helmut Schwab ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Structural Diversity ◽  
Kinetic Properties ◽  
Hydrolysis Of

The construction of Cu(i)/Cu(ii) coordination polymers based on pyrazine–carboxylate: Structural diversity tuned by in situ hydrolysis reaction

CrystEngComm ◽  
2013 ◽  
Vol 15 (26) ◽  
pp. 5359 ◽  
Author(s):  
Zhen-Zhen Wen ◽  
Xi-Ling Wen ◽  
Song-Liang Cai ◽  
Sheng-Run Zheng ◽  
Jun Fan ◽  
...  
Keyword(s):  
Coordination Polymers ◽  
Structural Diversity ◽  
Hydrolysis Reaction

scholarly journals Kinetic Analysis of the Hydrolysis of Pentose-1-Phosphates Through Apparent Nucleoside Phosphorolysis Equilibrium Shifts

2020 ◽  
Author(s):  
Felix Kaspar ◽  
Peter Neubauer ◽  
Anke Kurreck
Keyword(s):  
Kinetic Analysis ◽  
Hydrolysis Kinetics ◽  
Equilibrium Thermodynamics ◽  
Apparent Increase ◽  
Equilibrium Conversion ◽  
Sugar Phosphates ◽  
Kinetics Of ◽  
Hydrolysis Of

<div>Ask what an equilibrium can do for you:</div><div>Hydrolysis of pentose-1-phosphates leads to an apparent increase of the equilibrium conversion in nucleoside phosphorolysis reactions. This information can be leveraged via equilibrium thermodynamics to determine the hydrolysis kinetics of in situ generated sugar phosphates, which are known to be elusive and difficult to quantify.<br></div>

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