scholarly journals New Biomarkers of Kawasaki Disease Identified by Gingival Crevicular Fluid Proteomics

2021 ◽  
Author(s):  
Xue Fan ◽  
Ying Li ◽  
Xin Guo ◽  
Jingjing Lan ◽  
Mingguo Xu
Keyword(s):  
Mass Spectrometry ◽  
Kawasaki Disease ◽  
Quantitative Proteomics ◽  
Gingival Crevicular Fluid ◽  
Expression Profiles ◽  
Mass Spectrometry Analysis ◽  
Ivig Treatment ◽  
Control Group ◽  
New Biomarkers ◽  
Crevicular Fluid

Abstract Background: Kawasaki Disease (KD) is an acute self-limiting systemic vasculitis syndrome which can result in arterial damage especially in coronary artery. To find possible new biomarkers for the diagnosis of KD by Data independent acquisition (DIA) quantitative proteomics.Methods: Twenty-seven patients with KD were enrolled in the present study, further, gingival crevicular fluid of before IVIG treatment with KD was collected as the experimental group. Meanwhile, 18 healthy volunteers were recruited as the control group.DIA quantitative proteomics mass spectrometry analysis was performed on the GCF samples of each group, and the protein expression profiles of the two groups of GCF were detected. Function enrichment of DEP by KEGG and GO, protein-protein interaction (PPI) analysis for all the DEPs detected, Finally, the multiple reaction monitoring mass spectrometry method was used to verify the selected DEPs.Results:197 DEPs (174 up-regulated and 23 down-regulated) were detected in the CGF between the KD group and the normal control group. Cellular process and metabolic process, binding and catalytic activity are the most altered biological process and molecular function, respectively. NOD−like receptor signaling pathway, Protein processing in endoplasmic reticulum pathway and influenza pathway are most significant pathway. EIF2AK2, B2M and GBP1 are kernel proteins in PPI network. The results of MRM-MS of 12 DEPs including IFIT3, UB2L6, HP, A1AT, HSP90AA1, HNRPC, HSP90AB1, SAA1, MX1, B2M, FKBP4 and TRAP1 were highly consistent with DIA.Conclusions: We suggested that 12 proteins in GCF could be used as new biomarkers for early diagnosis of KD. We also found that KD is closely related to gingival inflammation at the molecular level, which provides new ideas and directions for the diagnosis and treatment of KD.

scholarly journals New Biomarkers of Kawasaki Disease Identified by Gingival Crevicular Fluid Proteomics

2021 ◽  
Author(s):  
Xue Fan ◽  
Ying Li ◽  
Xin Guo ◽  
Jingjing Lan ◽  
Mingguo Xu
Keyword(s):  
Mass Spectrometry ◽  
Kawasaki Disease ◽  
Quantitative Proteomics ◽  
Gingival Crevicular Fluid ◽  
Expression Profiles ◽  
Mass Spectrometry Analysis ◽  
Ivig Treatment ◽  
Control Group ◽  
New Biomarkers ◽  
Crevicular Fluid

Abstract Background Kawasaki Disease (KD) is an acute self-limiting systemic vasculitis syndrome which can result in arterial damage especially in coronary artery. To find possible new biomarkers for the diagnosis of KD by Data independent acquisition (DIA) quantitative proteomics. Methods Twenty-seven patients with KD were enrolled in the present study, further, gingival crevicular fluid of before IVIG treatment with KD was collected as the experimental group. Meanwhile, 18 healthy volunteers were recruited as the control group.DIA quantitative proteomics mass spectrometry analysis was performed on the GCF samples of each group, and the protein expression profiles of the two groups of GCF were detected. Function enrichment of DEP by KEGG and GO, protein-protein interaction (PPI) analysis for all the DEPs detected, Finally, the multiple reaction monitoring mass spectrometry method was used to verify the selected DEPs. Results 197 DEPs (174 up-regulated and 23 down-regulated) were detected in the CGF between the KD group and the normal control group. Cellular process and metabolic process, binding and catalytic activity are the most altered biological process and molecular function, respectively. NOD−like receptor signaling pathway, Protein processing in endoplasmic reticulum pathway and influenza pathway are most significant pathway. EIF2AK2, B2M and GBP1 are kernel proteins in PPI network. The results of MRM-MS of 12 DEPs including IFIT3, UB2L6, HP, A1AT, HSP90AA1, HNRPC, HSP90AB1, SAA1, MX1, B2M, FKBP4 and TRAP1 were highly consistent with DIA. Conclusions We suggested that 12 proteins in GCF could be used as new biomarkers for early diagnosis of KD. We also found that KD is closely related to gingival inflammation at the molecular level, which provides new ideas and directions for the diagnosis and treatment of KD.

Mass spectrometry analysis of gingival crevicular fluid in the presence of external root resorption

2014 ◽  
Vol 145 (6) ◽  
pp. 787-798 ◽  
Author(s):  
Wellington J. Rody Jr ◽  
L. Shannon Holliday ◽  
Kevin P. McHugh ◽  
Shannon M. Wallet ◽  
Victor Spicer ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Root Resorption ◽  
Gingival Crevicular Fluid ◽  
Mass Spectrometry Analysis ◽  
Spectrometry Analysis ◽  
External Root Resorption ◽  
Crevicular Fluid

scholarly journals Are Proteinase 3 and Cathepsin C Enzymes Related to Pathogenesis of Periodontitis?

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Oya Türkoğlu ◽  
Elif Azarsız ◽  
Gülnur Emingil ◽  
Necil Kütükçüler ◽  
Gül Atilla
Keyword(s):  
Gingival Crevicular Fluid ◽  
Periodontal Diseases ◽  
Study Groups ◽  
Aggressive Periodontitis ◽  
Control Group ◽  
Proteinase 3 ◽  
Cathepsin C ◽  
Probing Depth ◽  
Inflammatory Processes ◽  
Crevicular Fluid

Aim. Cathepsin C is the activator of the polymorphonuclear leukocyte-derived proteinase 3, which contributes to inflammatory processes. The aim of the present study was to investigate gingival crevicular fluid (GCF) proteinase 3 and cathepsin C levels in periodontal diseases.Design. Eighteen patients with chronic periodontitis (CP), 20 patients with generalized aggressive periodontitis (G-AgP), 20 patients with gingivitis, and 18 healthy subjects were included in the study. Periodontal parameters including probing depth, clinical attachment level, papilla bleeding index, and plaque index were assessed in all study subjects. GCF proteinase 3 and cathepsin C levels were analyzed by ELISA.Results. GCF proteinase 3 total amount was significantly higher in diseased groups compared to control group, after adjusting ageP<0.05. No differences were found in GCF cathepsin C levels among the study groupsP>0.05. Periodontal parameters of sampling sites were positively correlated with GCF proteinase 3 total amountsP<0.01but not with cathepsin C total amountsP>0.05.Conclusions. Elevated levels of GCF proteinase 3 in CP, G-AgP, and gingivitis might suggest that proteinase 3 plays a role during inflammatory periodontal events in host response. However, cathepsin C in GCF does not seem to have an effect on the pathogenesis of periodontal diseases.

scholarly journals Metabolomic Analysis of Gingival Crevicular Fluid Using Gas Chromatography/Mass Spectrometry

2016 ◽  
Vol 5 (1) ◽  
pp. A0047-A0047 ◽  
Author(s):  
Miho Ozeki ◽  
Takenori Nozaki ◽  
Jun Aoki ◽  
Takeshi Bamba ◽  
Kirk R. Jensen ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Gingival Crevicular Fluid ◽  
Gas Chromatography Mass Spectrometry ◽  
Metabolomic Analysis ◽  
Chromatography Mass Spectrometry ◽  
Crevicular Fluid

scholarly journals THE EFFECTS OF PANORAMIC RADIOGRAPHY ON GINGIVAL CREVICULAR FLUID VOLUME IN GINGIVITIS

2021 ◽  
Vol 6 (2) ◽  
pp. 136
Author(s):  
Rini Rahmiyati ◽  
Didit Aspriyanto ◽  
Beta Widya Oktiani
Keyword(s):  
Filter Paper ◽  
Wistar Rats ◽  
Gingival Crevicular Fluid ◽  
Panoramic Radiography ◽  
Cell Damage ◽  
Biological Fluid ◽  
Control Group ◽  
Control Group Design ◽  
Significant Difference ◽  
Crevicular Fluid

Background: Gingival crevicular fluid (GCF) is a biological fluid derived from the gingival sulcus and can be elevated in the inflammatory state of periodontal tissue, such as gingivitis. In previous studies, the number of GCF could also increase after panoramic radiographic exposure. Increase in GCF due to panoramic radiography is a sign of cell damage. Objective: To analyze the effects of panoramic radiography on the volume of GCF in wistar rats with gingivitis. Method: This type of research was true experimental with post test only and control group design. The sampling technique used was simple random sampling. A total of 25 wistar rats were classified into two control groups without exposure (state without gingivitis and gingivitis) and three groups treatment of exposure (state of gingivitis with 1 time, 2 times, and 3 times the exposure). GCF sampling using filter paper was carried out 10 minutes after panoramic radiographic exposure. The filter paper was stained by 2% ninhydrin solution, after that the GCF volume was calculated. Results: There was a significant difference in the number of GCF (p<0.05) in the group without gingivitis and exposure compared to all other groups, the gingivitis group without exposure compared to the gingivitis group with 3 times exposure, and the gingivitis group with 1 time exposure compared to the gingivitis group 3 times with exposure. Conclusion: Panoramic radiography can cause an increase in the volume of GCF in wistar rats with gingivitis. Keywords: GCF, gingivitis, panoramic radiography

scholarly journals Modulatory Effect of Guinep (Melicoccus bijugatus Jacq) Fruit Pulp Extract on Isoproterenol-Induced Myocardial Damage in Rats. Identification of Major Metabolites Using High Resolution UHPLC Q-Orbitrap Mass Spectrometry

Molecules ◽  
2019 ◽  
Vol 24 (2) ◽  
pp. 235 ◽  
Author(s):  
Chukwuemeka Nwokocha ◽  
Isheba Warren ◽  
Javier Palacios ◽  
Mario Simirgiotis ◽  
Magdalene Nwokocha ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Resolution ◽  
Myocardial Injury ◽  
Weight Ratio ◽  
Mass Spectrometry Analysis ◽  
Control Group ◽  
Qtc Interval ◽  
Protective Effects ◽  
Orbitrap Mass Spectrometry ◽  
Hematological Analysis

Guinep is traditionally used in the management of cardiovascular ailments. This study aims to evaluate its medicinal constituents and effects in the management of myocardial injury in an experimental isoproterenol (ISO) rat model. Sprague-Dawley rats were randomly assigned to four groups: Group 1 was the control group; Group 2 received M. bijugatus extract (100 mg/Kg; MB) for six weeks; Group 3 was given ISO (85 mg/Kg) i.p. twice during a 24-hour period; and Group 4 was given ISO (85 mg/Kg) i.p. and MB extract (100 mg/Kg) for six weeks. The MB was administered orally by gavage, daily. The blood pressure of conscious animals was measured, while ECG was performed under anesthesia. Blood and serum were collected for biochemical and hematological analysis. The ISO group treated with MB showed a significant decrease (p < 0.001) in (SBP), diastolic (DBP), mean arterial (MAP) and heart rate (HR) compared to the ISO only group. Conversely, MB treated rats that were not induced with ISO displayed a significant decreases (p < 0.001) in SBP, DBP, MAP, and HR. ISO significantly elevated the ST segment (p < 0.001) and shortened the QTc interval (p < 0.05), which were recovered after treatment with 100 mg/Kg of MB. In addition, the results showed a significant decrease (p < 0.001) in the heart to body weight ratio of the ISO group treated with MB compared to the ISO only group. Furthermore, the extract normalized the hematological values depressed by the ISO while significantly elevating the platelet count. UHPLC high-resolution orbitrap mass spectrometry analysis results revealed the presence of several antioxidants like vitamin C and related compounds, phenolic acids, flavonoid, fatty acids (oxylipins), and terpene derivatives. The results of this study indicated that Melicoccus bijugatus did display some cardio-protective effects in relation to myocardial injury.

scholarly journals Analysis of gingival crevicular fluid with mass spectrometry

2007 ◽  
Vol 52 (S4) ◽  
pp. S25-S26
Author(s):  
LH Ngo ◽  
PD Veith ◽  
IB Darby ◽  
EC Reynolds
Keyword(s):  
Mass Spectrometry ◽  
Gingival Crevicular Fluid ◽  
Crevicular Fluid

scholarly journals Effects of Standardised Fermented Papaya Gel on Clinical Symptoms, Inflammatory Cytokines, and Nitric Oxide Metabolites in Patients with Chronic Periodontitis: An Open Randomised Clinical Study

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Zaira F. Kharaeva ◽  
Lyana R. Zhanimova ◽  
Magomet Sh. Mustafaev ◽  
Chiara De Luca ◽  
Wolfgang Mayer ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Gingival Crevicular Fluid ◽  
Clinical Symptoms ◽  
Topical Administration ◽  
Control Group ◽  
Pocket Depth ◽  
Bacterial Killing ◽  
Anti Inflammatory ◽  
Nitric Oxide Metabolites ◽  
Crevicular Fluid

The clinical efficacy of topical administration of standardised fermented papaya gel (SFPG), known to have antioxidant and anti-inflammatory properties,versusconventional therapy was evaluated in a group of 84 patients with moderate-to-severe periodontitis, randomly assigned to control group (n=45) undergoing traditional pharmacologic/surgical protocols or to experimental group (n=39), additionally treated with intragingival pocket SFPG (7 g) applications (15 min daily for 10 days). Patients undergoing SFPG treatment showed significant (P<0.05), durable improvement of three major clinical indices of disease severity: reduced bleeding (day 7), plaque and gingival conditions (day 14), and consistent gingival pocket depth reduction (day 45). Proinflammatory nitric oxide metabolites reached normal values in plasma (day 14) and gingival crevicular fluid (GCF) at day 45 with SFPG applications compared to controls that did not reach normalisation. Levels of highly increased proinflammatory (IL-1B, IL-6) and suppressed anti-inflammatory (IL-10) cytokines normalised in the SFPG group by days 14 (plasma) and 45 (GCF), but never in the control group. Although not acting directly as antibiotic, SFPG acted in synergy with human granulocytes blocking adaptive catalase induction inS. aureusin response to granulocyte-derived oxidative stress, thus enhancing intracellular bacterial killing.

scholarly journals The Effects of Salvia officinalis Gel as an Adjunct to Scaling and Root Planning in Patients with Periodontitis (Clinical and Immunological Study)

2020 ◽  
Vol 10 (02) ◽  
pp. 232-237
Author(s):  
Ismael W. Aljuboori ◽  
Maha Sh. Mahmood
Keyword(s):  
Transforming Growth Factor Beta ◽  
Chronic Periodontitis ◽  
Transforming Growth Factor ◽  
Gingival Crevicular Fluid ◽  
Test Group ◽  
Control Group ◽  
Growth Factor Beta ◽  
Crevicular Fluid ◽  
Root Planning ◽  
Scaling And Root Planning

Background: Phytotherapy is the usage of herbal species with medicinal properties for the management of various diseases. Gingivitis and periodontitis are diseases that involve the role of both the bacteria and the host immune response. Over the years, various researches have shown the importance of herbal products in the management of periodontal diseases. Aims of the study: To evaluate the efficacy of locally applied Salvia officinalis gel as adjunctive in the treatment of chronic periodontitis. Subjects and methods: Fourteen patients (10 males and 4 females) with chronic periodontitis were enrolled in the present study with total number of twenty-eight periodontal pockets utilizing a split mouth design, the pockets were divided into two groups, the test group which was treated with scaling and root planning procedure and the application of the S. officinalis gel, and the control group that treated with scaling and root planning only. Plaque index (PI) and gingival index (GI) were recorded for each site. Gingival crevicular fluid (GCF) was collected from each site by using PerioCol paper strips. The concentration of the transforming growth factor beta-1 in the gingival crevicular fluid was quantified by a high sensitivity enzyme-linked immunosorbent assay. Results: The test group demonstrated a significant reduction in GI at 1-week and 1-month after the treatment comparing to baseline (1.14 vs. 1.64, p = 0.003, and 1.21 vs. 1.64, p = 0.028, respectively), while no significant reduction in the PI at recall visits comparing to baseline. The control group demonstrated no significant reduction in PI and GI at recall visits, comparing to baseline. Both the test and control groups demonstrated no significant reduction in the transforming growth factor-beta 1 concentration in the gingival crevicular fluid at one week after the treatment comparing to baseline, while at one month after the treatment only the test group demonstrated a significant reduction comparing to baseline (3.91 vs. 9.62, p = 0.044). Conclusion: The findings of the present study indicated that the S. officinalis gel has a potential anti-inflammatory role in the treatment of chronic periodontitis by monitoring both the clinical and immunological parameters.

scholarly journals Changes in Matrix Metalloproteinase-8, Interleukin-6 and Tu-mor Necrosis Factor-Α in Gingival Crevicular Fluid during Rap-id Maxillary Expansion in Adolescent Patients

2021 ◽  
Author(s):  
Zhipeng Tang ◽  
Dongxue Mi ◽  
Hao Wu ◽  
Yanan Fu ◽  
Li Liu ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Gingival Crevicular Fluid ◽  
Control Group ◽  
Observation Group ◽  
Periodontal Tissue ◽  
Maxillary Expansion ◽  
Clinical Indicators ◽  
Tnf Α ◽  
Crevicular Fluid ◽  
Necrosis Factor

Background: Rapid maxillary expansion (RME) is the standard treatment for correcting lateral maxillary defects commonly used in orthodontics. It is the most effective approach to increase maxillary width in clinical practice. At present, there are few studies on the level of molecular biology of periodontal tissue remodeling during RME. We aimed to investigate changes in matrix metalloproteinase (MMP)-8, interleukin (IL)-6 and tumor necrosis factor (TNF)-αin gingival crevicular fluid during RME. Methods: Patients admitted to Department of Stomatology, the Third Affiliated Hospital of Qiqihar Medical University, Qiqihar, Heilongjiang Province, China between Dec 2016 and Dec 2018 were enrolled, and randomly divided into the observation group (76 cases) and control group (62 cases). Periodontal clinical indicators were recorded. Gingival crevicular fluid was collected and the periodontal clinical indicators were recorded. The levels of MMP-8, IL-6 and TNF-α were determined by ELISA, and the contents of the two groups were compared. Results: The plaque index of the observation group was significantly higher than that of the baseline T0 (P< 0.05) from T4, and the increase in the control group started from T5. The general clinical data of the two groups showed that the white blood cell count of the observation group was higher than that of the control group. The levels of MMP-8, IL-6 and TNF-α were the highest at T2, followed by T3, and gradually decreased at T4, and T5, and the differences were significant (P<0.05). Conclusion: The changes in levels of MMP-8, IL-6 and TNF-α in adolescent patients during RME were related to the remodeling of periodontal tissue after RME.

Sign in / Sign up

Export Citation Format

Share Document