Enzymology of Plant Cell Wall Breakdown by Plant Pathogens.

1979 ◽  
Author(s):  
Durward F. Bateman ◽  
James R. Aist
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Plant Pathogens ◽  
Plant Cell Wall ◽  
Cell Wall Breakdown

Enzymology of Plant Cell Wall Breakdown: An Update

2010 ◽  
pp. 73-96 ◽  
Author(s):  
Leonora R. S. Moreira ◽  
Natália vG. Milanezi ◽  
Edivaldo X. F. Filho
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Cell Wall Breakdown

scholarly journals Structure of a polyisoprenoid binding domain from Saccharophagus degradans implicated in plant cell wall breakdown

FEBS Letters ◽  
2010 ◽  
Vol 584 (8) ◽  
pp. 1577-1584 ◽  
Author(s):  
Florence Vincent ◽  
David Dal Molin ◽  
Ronald M. Weiner ◽  
Yves Bourne ◽  
Bernard Henrissat
Keyword(s):  
Cell Wall ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Binding Domain ◽  
Saccharophagus Degradans ◽  
Cell Wall Breakdown

scholarly journals The Endo-Arabinanase BcAra1 Is a Novel Host-Specific Virulence Factor of the Necrotic Fungal Phytopathogen Botrytis cinerea

2014 ◽  
Vol 27 (8) ◽  
pp. 781-792 ◽  
Author(s):  
Majse Nafisi ◽  
Maria Stranne ◽  
Lisha Zhang ◽  
Jan A. L. van Kan ◽  
Yumiko Sakuragi
Keyword(s):  
Cell Wall ◽  
Botrytis Cinerea ◽  
Plant Cell ◽  
Plant Pathogens ◽  
Plant Cell Wall ◽  
High Efficiency ◽  
Microbial Infection ◽  
Cell Wall Degrading Enzymes ◽  
Novel Host

The plant cell wall is one of the first physical interfaces encountered by plant pathogens and consists of polysaccharides, of which arabinan is an important constituent. During infection, the necrotrophic plant pathogen Botrytis cinerea secretes a cocktail of plant cell-wall-degrading enzymes, including endo-arabinanase activity, which carries out the breakdown of arabinan. The roles of arabinan and endo-arabinanases during microbial infection were thus far elusive. In this study, the gene Bcara1 encoding for a novel α-1,5-L-endo-arabinanase was identified and the heterologously expressed BcAra1 protein was shown to hydrolyze linear arabinan with high efficiency whereas little or no activity was observed against the other oligo- and polysaccharides tested. The Bcara1 knockout mutants displayed reduced arabinanase activity in vitro and severe retardation in secondary lesion formation during infection of Arabidopsis leaves. These results indicate that BcAra1 is a novel endo-arabinanase and plays an important role during the infection of Arabidopsis. Interestingly, the level of Bcara1 transcript was considerably lower during the infection of Nicotiana benthamiana compared with Arabidopsis and, consequently, the ΔBcara1 mutants showed the wild-type level of virulence on N. benthamiana leaves. These results support the conclusion that the expression of Bcara1 is host dependent and is a key determinant of the disease outcome.

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