Training Entitled Development and Characterization of Transgenic Mice With Mammary Gland Specific Expression of the Tumor Suppressor

2000 ◽  
Author(s):  
Bruce Cuevas
Keyword(s):  
Mammary Gland ◽  
Tumor Suppressor ◽  
Transgenic Mice ◽  
Specific Expression

scholarly journals Characterization of transgenic mice with neuron-specific expression of soluble epoxide hydrolase

2009 ◽  
Vol 1291 ◽  
pp. 60-72 ◽  
Author(s):  
Robert A. Bianco ◽  
Khristofor Agassandian ◽  
Martin D. Cassell ◽  
Arthur A. Spector ◽  
Curt D. Sigmund
Keyword(s):  
Transgenic Mice ◽  
Epoxide Hydrolase ◽  
Soluble Epoxide Hydrolase ◽  
Specific Expression

Expression and characterization of a lysine rich protein in cow milk

2018 ◽  
Author(s):  
Xin Ma ◽  
Li Su ◽  
Peng Zhang ◽  
Sheng Zhang ◽  
Bo Tang ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Amino Acid ◽  
Expression Vector ◽  
Cow Milk ◽  
Specific Expression ◽  
Mammary Gland Tissue ◽  
Gland Tissue ◽  
Negative Controls ◽  
Mammary Gland Expression

The lysine is considered as the most important essential amino acid, because it is the most limiting in the cereals grains. In this study, a lysine-rich (LR) gene, and the expression vector pcDNA3.1-LR and pBC1-LR were constructed. The LR was expressed in 293T cells driven by the vector pcDNA3.1-LR and checked by RT-PCR and WB. The mammary gland tissue-specific expression vector carrying the LR was injected directly into the lactating mammary glands of cows and the milk samples were checked by a complete amino acid analysis. The results showed that the LR protein was expressed successfully in cells and in cow milk; the expression of LR lasted for 6 d, and the lysine level of the injection group was significantly higher than that of negative controls (p Lass Than 0.05). This study provide a better understanding of how mammary gland expression systems increase the lysine content of milk that can be applied to transgenic dairy cow.

Molecular Characterization of the Mouse Tyrosinase Gene:Pigment Cell-Specific Expression in Transgenic Mice

1992 ◽  
Vol 5 (5) ◽  
pp. 295-299 ◽  
Author(s):  
FRIEDRICH BEERMANN ◽  
ERIKA SCHMID ◽  
RUTH GANSS ◽  
GUNTHER SCHUTZ3 ◽  
SIEGFRIED RUPPERT
Keyword(s):  
Transgenic Mice ◽  
Molecular Characterization ◽  
Specific Expression

Characterization of the ovine LHβ-subunit gene: the promoter directs gonadotrope-specific expression in transgenic mice

1993 ◽  
Vol 93 (2) ◽  
pp. 157-165 ◽  
Author(s):  
Pamela Brown ◽  
Judy R. Mcneilly ◽  
Roberta M. Wallace ◽  
Alan S. Mcneilly ◽  
A.John Clark
Keyword(s):  
Transgenic Mice ◽  
Subunit Gene ◽  
Specific Expression

Osteoblast-specific expression of growth hormone stimulates bone growth in transgenic mice

1992 ◽  
Vol 12 (12) ◽  
pp. 5541-5547
Author(s):  
A R Baker ◽  
P G Hollingshead ◽  
S Pitts-Meek ◽  
S Hansen ◽  
R Taylor ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Transgenic Mice ◽  
Bone Growth ◽  
Blot Hybridization ◽  
Postnatal Growth ◽  
Systemic Effects ◽  
Specific Expression ◽  
Important Regulator

Growth hormone (GH) is an important regulator of postnatal growth, acting on a wide variety of target tissues. Here, we show that local production of GH in osteoblasts is able to stimulate bone growth directly without significant systemic effects. Mice were made transgenic by microinjection of an osteocalcin-human GH (osteocalcin-hGH) gene construct in which approximately 1,800 bp of the rat osteocalcin promoter was fused to the hGH gene. Five lines of transgenic mice, each with measurable amounts of serum hGH (ranging from 1 to 1,000 ng/ml), were analyzed. Northern (RNA) blot hybridization showed that the hGH transcript was detectable only in the bone. Further characterization of hGH mRNA distribution by in situ hybridization revealed that in neonates the most intense signal was found in periosteal osteoblasts, while in adults, trabecular and endosteal osteoblasts were favored. In one transgenic line (992-1), hGH was expressed at a much lower level and had minimal systemic effects; however, the local concentrations of hGH in bone were sufficient to stimulate bone growth in these animals.

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