scholarly journals Barcoding and interspecific relationships of Macaronesian Weevils (Coleoptera: Curculionoidea)

2021 ◽  
Vol 71 (1) ◽  
pp. 127-135
Author(s):  
Peter E. Stüben ◽  
André Schütte ◽  
Jonas J. Astrin
Keyword(s):  
Species Identification ◽  
Single Case ◽  
Type Material ◽  
Similar Species ◽  
Dna Barcodes ◽  
Species Relationships ◽  
Genus Level ◽  
Reference Collection ◽  
Taxonomic Approach ◽  
Morphological Species

In an integrative taxonomic approach, this study presents mitochondrial CO1 barcodes for 468 of the 735 so far described Lauri-Macaronesian weevil (Curculionoidea) species and subspecies in 1388 samples. A Bayesian tree (electronic supplement) provides insights into within-species relationships, without aiming at phylogenetic accuracy above genus level. With the exception of a few discussed cases, the present dataset of DNA barcodes allows a reliable re-identification and remarkable differentiation of species and will accelerate the discovery of new weevil species from the Canary, Madeira and Azores archipelagos. Only in a single case, the similar species Rhinoncus castor and R. bruchoides (Ceutorhynchinae) from Madeira, did the molecular (re)identification reveal an unresolvable contradiction with our morphological species identification. In many cases, morphological determinations were bolstered by comparisons with the type material. Sequenced specimens were mounted and deposited in a reference collection for later re-determinations.

Redescription of three species of Apobaetis Day, 1955 (Ephemeroptera: Baetidae)

Zootaxa ◽  
2020 ◽  
Vol 4808 (2) ◽  
pp. 317-330
Author(s):  
PAULO VILELA CRUZ
Keyword(s):  
South America ◽  
Species Identification ◽  
Distinct Species ◽  
Identification Key ◽  
Type Material ◽  
Similar Species ◽  
Type Specimens ◽  
The Family ◽  
Morphological Diagnosis ◽  
Almost All

The genus Apobaetis Day is one of the few lineages of the family Baetidae distributed throughout the continental Americas. Three very similar species of this genus—Apobaetis etowah (Traver), Apobaetis signifer Lugo-Ortiz & McCaffety, and Apobaetis fiuzai Salles & Lugo-Ortiz—have important morphological details, pertinent to species identification, that remain to be described. This lack of detailed taxonomic information has resulted in ample misdiagnoses of the species, particularly regarding A. fiuzai in South America. In fact, almost all the recently collected specimens have been assigned to this species. The present study aimed to establish a new morphological diagnosis of these three Apobaetis species through a redescription of their larvae, based on type material, and the proposition of a new identification key for larvae of this genus. The review of the type specimens supports two conclusions: (i) that A. etowah, A. signifer, and A. fiuzai are highly similar, yet can be considered as distinct species, and (ii) the previous diagnoses and keys do not effectively separate A. fiuzai from A. signifer. 

Morphology and DNA barcodes of two species of Bradysia Winnertz from China (Diptera, Sciaridae), with the description of Bradysia minorlobus Yang, Shi & Huang sp. n.

Zootaxa ◽  
2019 ◽  
Vol 4612 (1) ◽  
pp. 85 ◽  
Author(s):  
XUE YANG ◽  
KAI SHI ◽  
KAI HELLER ◽  
FRANK MENZEL ◽  
JUNHAO HUANG ◽  
...  
Keyword(s):  
Morphological Characters ◽  
Species Group ◽  
Genetic Distances ◽  
Species Concepts ◽  
Similar Species ◽  
Dna Barcodes ◽  
Neighbor Joining ◽  
Coi Sequences ◽  
Morphological Species ◽  
Group Species

Two morphologically similar species of the fungicola species group of Bradysia Winnertz, 1867 were studied in China: Bradysia chenjinae Yang, Zhang & Yang, 1993 and Bradysia minorlobus Yang, Shi & Huang sp. n. The morphological species concepts were supported by the DNA barcodes of COI sequences. The genetic distances of 16 Bradysia fungicola group species were analyzed and a neighbor-joining tree was constructed. The morphological characters of both Chinese species were described and illustrated. 

Identity of the oribatid mite Oribata curva and transfer to Trichogalumna (Acari, Oribatida, Galumnidae), with discussion of nomenclatural and biogeographical issues in the ‘curva’ species-group

Zootaxa ◽  
2017 ◽  
Vol 4272 (4) ◽  
pp. 551
Author(s):  
ROY A. NORTON ◽  
SERGEY G. ERMILOV
Keyword(s):  
North America ◽  
Oribatid Mite ◽  
Species Group ◽  
Type Material ◽  
Similar Species ◽  
History Of ◽  
Molecular Assessment

Based on the study of type material, other historical specimens, and new collections, the adult of the thelytokous oribatid mite Oribata curva Ewing, 1907 (Galumnidae) is redescribed and the name is recombined to Trichogalumna curva (Ewing, 1907) comb. nov. A confusing history of synonymies and misidentifications is traced in detail, and their effect on published statements about biogeography is assessed. Reliable records of T. curva are only those from North America. The tropical mite Pergalumna ventralis (Willmann, 1932) is not a subspecies of T. curva. The widely-reported Trichogalumna nipponica (Aoki, 1966) and other similar species form a complex with T. curva that needs further morphological and molecular assessment. 

scholarly journals Benchmarking DNA barcodes: an assessment using available primate sequences

Genome ◽  
2006 ◽  
Vol 49 (7) ◽  
pp. 851-854 ◽  
Author(s):  
Mehrdad Hajibabaei ◽  
Gregory AC Singer ◽  
Donal A Hickey
Keyword(s):  
New Species ◽  
Cryptic Species ◽  
Dna Barcoding ◽  
Species Identification ◽  
Dna Sequences ◽  
Phylogenetic Relationships ◽  
Primate Species ◽  
Dna Barcodes ◽  
Global Biodiversity ◽  
Efficient Sequence

DNA barcoding has been recently promoted as a method for both assigning specimens to known species and for discovering new and cryptic species. Here we test both the potential and the limitations of DNA barcodes by analysing a group of well-studied organisms—the primates. Our results show that DNA barcodes provide enough information to efficiently identify and delineate primate species, but that they cannot reliably uncover many of the deeper phylogenetic relationships. Our conclusion is that these short DNA sequences do not contain enough information to build reliable molecular phylogenies or define new species, but that they can provide efficient sequence tags for assigning unknown specimens to known species. As such, DNA barcoding provides enormous potential for use in global biodiversity studies.Key words: DNA barcoding, species identification, primate, biodiversity.

scholarly journals Tusk or Bone? An Example of Ivory Substitute in the Wildlife Trade

2011 ◽  
Vol 2 ◽  
pp. 40-44 ◽  
Author(s):  
Margaret E. Sims ◽  
Barry W. Baker ◽  
Robert M. Hoesch
Keyword(s):  
Material Culture ◽  
Species Identification ◽  
Dna Analysis ◽  
Wildlife Trade ◽  
Bone Tools ◽  
Wildlife Forensics ◽  
Ancient Dna Analysis ◽  
Modern Context ◽  
Morphological Species ◽  
Ivory Trade

Bone carvings (and other ivory substitutes) are common in the modern-day lucrative international ivory trade.  Souvenirs for unknowing travelers and market shoppers can be made of non-biological material (plastic "ivory" beads) or skillfully crafted natural objects made to resemble something other than their true origin.  Many of these items are received at the U. S. National Fish and Wildlife Forensics Laboratory (NFWFL) for species identification as part of law enforcement investigations.  Morphologists at the Lab often receive uniquely carved ivory items that have been imported with little or no documentation.  In recent years, analysts examined several purported ivory tusks suspected to be walrus, a protected marine mammal.  After examination, the Lab determined their origin as carved leg bones of cattle using principles and methods of zooarchaeology and ancient DNA analysis.  The naturally long and straight ungulate metapodials had been cut, carved, filled, stained, and polished to closely resemble unmodified ivory tusks.  Morphological species identification of these bones proved to be a challenge since diagnostic characters of the bones had been altered and country of origin was unknown. Genetic analysis showed that the bones originated from cattle.  While bone is commonly used as a substitute for ivory, this style of artifact was not previously documented in the wildlife trade prior to our analysis.  Archaeological ethnobiologists commonly encounter bone tools and other forms of material culture from prehistoric and historic contexts; in this case bone tools come from a modern context, thus the application of methods common in zooarchaeology are situated in wildlife forensics.  In addition, results reported here pertain to cross-cultural ivory trade and conservation science.

BarcodingR: an integrated r package for species identification using DNA barcodes

2016 ◽  
Vol 8 (5) ◽  
pp. 627-634 ◽  
Author(s):  
Ai‐bing Zhang ◽  
Meng‐di Hao ◽  
Cai‐qing Yang ◽  
Zhi‐yong Shi
Keyword(s):  
Species Identification ◽  
R Package ◽  
Dna Barcodes

scholarly journals A new species of Oomyzus Rondani (Hymenoptera, Eulophidae) reared from the pupae of Coccinella septempunctata (Coleoptera, Coccinellidae) in China

ZooKeys ◽  
2020 ◽  
Vol 953 ◽  
pp. 49-60
Author(s):  
Hai-Tian Song ◽  
Ming-Hui Fei ◽  
Bao-Ping Li ◽  
Chao-Dong Zhu ◽  
Huan-Xi Cao
Keyword(s):  
New Species ◽  
Coccinella Septempunctata ◽  
Similar Species ◽  
Dna Barcodes ◽  
A New Species

Oomyzus spiraculus Song, Fei & Cao sp. nov. (Hymenoptera, Eulophidae) is described and illustrated as a gregarious larval-pupal endoparasitoid of Coccinella septempunctata L. (Coleoptera, Coccinellidae). Differentiation between O. spiraculus and its similar species is discussed and a key to differentiate the female and male of these species is provided. DNA barcodes of O. spiraculus and O. scaposus are analyzed and compared.

scholarly journals Environmental DNA (eDNA) Metabarcoding in the Fish Market and Nearby Seafood Restaurants in Taiwan Reveals the Underestimation of Fish Species Diversity in Seafood

Biology ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1132
Author(s):  
Hung-Tai Lee ◽  
Cheng-Hsin Liao ◽  
Te-Hua Hsu
Keyword(s):  
Species Diversity ◽  
Species Identification ◽  
Fish Species ◽  
Large Scale ◽  
Environmental Dna ◽  
Universal Primers ◽  
Similar Species ◽  
Operational Taxonomic Units ◽  
Fish Market ◽  
Fish Species Identification

Seafood, especially in traditional food Taiwan, is rarely sourced from a fixed species and routinely from similar species depending on their availability. Hence, the species composition of seafood can be complicated. While a DNA-based approach has been routinely utilized for species identification, a large scale of seafood identification in fish markets and restaurants could be challenging (e.g., elevated cost and time-consuming only for a limited number of species identification). In the present study, we aimed to identify the majority of fish species potentially consumed in fish markets and nearby seafood restaurants using environmental DNA (eDNA) metabarcoding. Four eDNA samplings from a local fish market and nearby seafood restaurants were conducted using Sterivex cartridges. Nineteen universal primers previously validated for fish species identification were utilized to amplify the fragments of mitochondrial DNA (12S, COI, ND5) of species in eDNA samples and sequenced with NovaSeq 6000 sequencing. A total of 153 fish species have been identified based on 417 fish related operational taxonomic units (OTUs) generated from 50,534,995 reads. Principal Coordinate Analysis (PCoA) further showed the differences in fish species between the sampling times and sampling sites. Of these fish species, 22 chondrichthyan fish, 14 Anguilliformes species, and 15 Serranidae species were respectively associated with smoked sharks, braised moray eels, and grouper fish soups. To our best knowledge, this work represents the first study to demonstrate the feasibility of a large scale of seafood identification using eDNA metabarcoding approach. Our findings also imply the species diversity in traditional seafood might be seriously underestimated and crucial for the conservation and management of marine resources.

scholarly journals Relevance of wing morphology in distinguishing and classifying genera and species of Stenogastrinae wasps

2011 ◽  
Vol 80 (3) ◽  
pp. 191-199 ◽  
Author(s):  
David Baracchi ◽  
Leonardo Dapporto ◽  
Stefano Turillazzi
Keyword(s):  
Geometric Morphometrics ◽  
Species Identification ◽  
Reliable Method ◽  
Wing Morphology ◽  
Wing Vein ◽  
Statistical Assessment ◽  
Genus Level ◽  
Nesting Material ◽  
First Time

The phylogeny of the Stenogastrinae wasps is still under discussion and their systematic incomplete. In the present work we used geometric morphometrics, a technique based on a rigorous statistical assessment of shape, to compare the forewings of fifteen species of Stenogastrinae wasps belonging to four different genera to ascertain whether this approach may be used as a reliable method in the study of the taxonomy of the group. The results show that the wing vein junctions can be diagnostic for both genus and species identification. For the first time in this subfamily, we propose a phylogenetic classification of the species based on wing morphology that largely agrees with the cladistic data available at genus level and reflects the differences among species in terms of nesting material and architecture of their nest.

scholarly journals DNA barcoding unveils the first record of Andrena allosa for Italy and unexpected genetic diversity in Andrena praecox (Hymenoptera: Andrenidae)

2020 ◽  
Vol 52 (1) ◽  
pp. 71-75
Author(s):  
Maurizio Cornalba ◽  
Paolo Biella ◽  
Andrea Galimberti
Keyword(s):  
Genetic Diversity ◽  
Global Change ◽  
Dna Barcoding ◽  
Species Identification ◽  
First Record ◽  
Global Distribution ◽  
Genetic Identification ◽  
Genetic Structuring ◽  
Eastern Sector ◽  
Morphological Species

DNA barcoding is well-known to support morphological species identification and it can be helpful for unveiling unexpected populations divergence patterns, especially in the context of the impacts on species posed by global change. In this note, we provided the first Italian record of the alpine mining bee Andrena allosa Warncke, 1975, confirmed with DNA barcoding. In addition, genetic identification of a specimen of Andrena praecox (Scopoli 1753) from western Italy pointed to an unexpected intraspecific genetic structuring at COI DNA barcoding region, with sequences from the Italian and the western sector of its global distribution differing 2.22% (p-dist) from populations of the eastern sector. Given the relevance of these records and of the genetic identity of bee populations from Italy, we argue that implementing molecular surveys in bee monitoring would surely contribute to the conservation of these important pollinators.

Sign in / Sign up

Export Citation Format

Share Document