scholarly journals Restriction Fragment Length Polymorphisms in Rose and Their Use for Cultivar Identification

HortScience ◽  
1992 ◽  
Vol 27 (2) ◽  
pp. 172-173 ◽  
Author(s):  
M. Hubbard ◽  
J. Kelly ◽  
S. Rajapakse ◽  
A. Abbott ◽  
R. Ballard
Keyword(s):  
Genetic Markers ◽  
Restriction Fragment ◽  
Fragment Length ◽  
Patent Protection ◽  
Cultivar Identification ◽  
Restriction Fragment Length Polymorphisms ◽  
Length Polymorphisms ◽  
Patent Rights ◽  
New Cultivars ◽  
Restriction Fragment Length

We have identified cloned rose DNA fragments that detect restriction fragment length polymorphisms (RFLP) in rose (Rosa ×hybrida) cultivars. RFLP can be used as genetic markers for identification, certification, and patent protection. By comparing RFLP patterns for each of six probes, we have been able to characterize eight cultivars. These results confirm that RFLP analyses are useful for rose cultivar identification and may provide a means for protecting patent rights to new cultivars.

scholarly journals IDENTIFICATION OF ROSE CULTIVARS BY RESTRICTION FRAGMENT LENGTH POLYMORPHISMS

HortScience ◽  
1991 ◽  
Vol 26 (5) ◽  
pp. 484b-484
Author(s):  
Sriyani Rajapakse ◽  
Mark Hubbard ◽  
Albert Abbott ◽  
Robert Ballard ◽  
John Kelly
Keyword(s):  
Restriction Fragment ◽  
Fragment Length ◽  
Genomic Dna ◽  
Cultivar Identification ◽  
Restriction Fragment Length Polymorphisms ◽  
Repeated Sequences ◽  
Dna Library ◽  
Dichotomous Key ◽  
Length Polymorphisms ◽  
Restriction Fragment Length

Restriction Fragment Length Polymorphisms (RFLPs) were investigated in rose cultivars as a means of reliable cultivar identification. A random genomic DNA library was generated by shotgun cloning HindIII digested fragments of DNA extracted from rose cultivar Confection into pUC8 plasmid of Escherichia coli strain JM 83. Compared to genomic clones carrying low or highly repeated sequences, clones with moderately repeated sequences were most effective in cultivar identification. These clones were identified by hybridizing rose DNA fragments from the library with genomic DNA from `Confection'. Clones with moderately repeated copy sequences were used as probes to detect the presence of RFLPs by Southern hybridization of EcoRI digested genomic DNA of various rose cultivars. Several of these probes have revealed RFLPs useful in cultivar identification. By using a combination of two or more of these probes most of the rose cultivars compared at this time can be identified. A dichotomous key useful in identification of rose cultivars was prepared from RFLPs displayed by 3A9 probe.

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