scholarly journals 3K1446 Dynamic behavior of nucleus and intranuclear DNA induced with laser nano-dissection of stress fibers in vascular smooth muscle cells(Cell biology 4,The 49th Annual Meeting of the Biophysical Society of Japan)

2011 ◽  
Vol 51 (supplement) ◽  
pp. S147
Author(s):  
Kazuaki Nagayama ◽  
Yuki Yahiro ◽  
Takeo Matsumoto
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Annual Meeting ◽  
Dynamic Behavior ◽  
Vascular Smooth Muscle Cells ◽  
Cell Biology ◽  
Muscle Cells ◽  
Stress Fibers ◽  
Biophysical Society

scholarly journals Lipopolysaccharide Acting Via Toll Like Receptor 4 Transactivates The TGF-β Receptor in Vascular Smooth Muscle cells

2021 ◽  
Author(s):  
Rizwana Afroz ◽  
Hirushi Kumarapperuma ◽  
Raafat Mohamad ◽  
Peter J Little ◽  
Danielle Kamato
Keyword(s):  
Transcription Factor ◽  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Cell Biology ◽  
Transforming Growth Factor ◽  
Muscle Cells ◽  
Host Cells ◽  
Β Receptor

Abstract Toll-like receptors (TLRs) recognise pathogen‑associated molecular patterns, which allow the detection of microbial infection by host cells. Bacterial derived toxin lipopolysaccharide activates TLR4 and leads to the activation of the Smad2 transcription factor. The phosphorylation of the Smad2 transcription factor is the result of the activation of the transforming growth factor-β receptor 1 (TGFBR1). Therefore, we sought to investigate LPS via TLR4 mediated Smad2C phosphorylation dependent on the (trans)activation of the TGFBR1. The invitro used human aortic vascular smooth muscle cells (HA-VSMCs) to assess the implications of TLR4 (trans)activation of the TGFBR1 in vascular pathophysiology. We show that LPS mediated Smad2 carboxy-terminal phosphorylation is inhibited in the presence of TGFBR1 inhibitor SB431542 in HA-VSMCs. Treatment with MyD88 and TRIF pathways antagonists does not affect LPS mediated phosphorylation of Smad2C; however, LPS mediated Smad2 phosphorylation was inhibited in the presence of MMP inhibitor, GM6001 and unaffected in the presence of ROCK inhibitor Y27632. LPS via transactivation of the TGFBR1 stimulates PAI-1 mRNA expression. TLRs are first in line to respond to exogenous invading substances and endogenous molecules; our findings characterise a novel signalling pathway in the context of cell biology. Identifying TLR transactivation of the TGFBR1 may provide future insight into the detrimental implications of pathogens in pathophysiology.

scholarly journals Imaging of Vascular Smooth Muscle Cells with Soft X-Ray Spectromicroscopy

2011 ◽  
Vol 17 (6) ◽  
pp. 991-1001 ◽  
Author(s):  
Julia Sedlmair ◽  
Sophie-Charlotte Gleber ◽  
Semra Öztürk Mert ◽  
Michael Bertilson ◽  
Olov von Hofsten ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells ◽  
Stress Fibers ◽  
X Ray ◽  
Compact Source ◽  
Scanning Transmission ◽  
Fixation Techniques

AbstractUsing X-ray microscopy and spectromicroscopy, vascular smooth muscle cells (VSMCs) were imaged, prepared without using additional embedding material or staining, but by applying simple, noncryo fixation techniques. The cells were imaged with a compact source transmission X-ray microscope and a scanning transmission X-ray microscope (STXM). With the STXM, spectromicroscopy was performed at the C K-edge and the Ca LIII,II-edges. VSMCs were chosen because of their high amount of actin stress fibers, so that the actin cytoskeleton should be visible. Other parts of the cell, such as the nucleus and organelles, were also identified from the micrographs. Both in the spectra and the images, the effects of the different preparation procedures were observable. Furthermore, Ca hotspots were detected and their density is determined.

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