scholarly journals Investigation of circulating DNA integrity after blood collection

BioTechniques ◽  
2021 ◽  
Author(s):  
Oriane Cédile ◽  
Sólja Remisdóttir Veyhe ◽  
Marcus Høy Hansen ◽  
Kjell Titlestad ◽  
Charlotte Guldborg Nyvold
Keyword(s):  
Blood Plasma ◽  
Quantitative Pcr ◽  
Blood Collection ◽  
Dna Integrity ◽  
Circulating Dna ◽  
In Blood Plasma

Method summary Concentrations of circulating DNA in blood plasma were compared using NanoDrop, Qubit, quantitative PCR and Bioanalyzer, and DNA integrity was evaluated with the Bioanalyzer according to the time of plasma preparation.

Deoxyribonuclease Activity and Circulating DNA Concentration in Blood Plasma of Patients with Prostate Tumors

2008 ◽  
Vol 1137 (1) ◽  
pp. 218-221 ◽  
Author(s):  
Anna V. Cherepanova ◽  
Svetlana N. Tamkovich ◽  
Olga E. Bryzgunova ◽  
Valentin V. Vlassov ◽  
Pavel P. Laktionov
Keyword(s):  
Blood Plasma ◽  
Circulating Dna ◽  
Prostate Tumors ◽  
Dna Concentration ◽  
Deoxyribonuclease Activity ◽  
In Blood Plasma

PLASMA LEVELS OF ESTRONE SULFATE, ESTRONE AND ESTRADIOL-17β IN THE COW AROUND PARTURITION

1975 ◽  
Vol 55 (4) ◽  
pp. 509-512 ◽  
Author(s):  
C. P. W. TSANG ◽  
A. J. HACKETT ◽  
E. M. TURNER Jr.
Keyword(s):  
Blood Plasma ◽  
Plasma Levels ◽  
Late Pregnancy ◽  
Blood Collection ◽  
Estrone Sulfate ◽  
Individual Variations ◽  
Before And After ◽  
The Mean ◽  
Estradiol 17Β ◽  
In Blood Plasma

Estrone sulfate, estrone, and estradiol-17β have been assayed in blood plasma taken from the mammary vein of five pregnant cows before and after parturition. While there were considerable individual variations, the mean plasma levels of estrone sulfate (13.4 ng/ml), estrone (1 ng/ml) and estradiol-17β (1 ng/ml) remained fairly constant over the period of blood collection prior to parturition (−88 h to −2 h). The levels of all three estrogens dropped rapidly within 8 h postpartum. It is concluded that estrone sulfate, rather than estrone, is the predominant plasma estrogen in late pregnancy in the cow.

scholarly journals Comparison of Sex Steroid Concentration in Blood Plasma and Ovarian Follicles of White Leghorn and Greenleg Partridge Laying Hens

2011 ◽  
Vol 11 (4) ◽  
pp. 507-520 ◽  
Author(s):  
Andrzej Sechman ◽  
Mirosława Staruszczak ◽  
Janusz Rząsa
Keyword(s):  
Blood Plasma ◽  
Laying Hens ◽  
Average Rate ◽  
Ovarian Follicles ◽  
Sex Steroid ◽  
Blood Collection ◽  
White Leghorn ◽  
Laying Performance ◽  
Reverse Relationship ◽  
In Blood Plasma

Comparison of Sex Steroid Concentration in Blood Plasma and Ovarian Follicles of White Leghorn and Greenleg Partridge Laying HensThe study was performed to compare plasma and ovarian levels of progesterone (P4) and estradiol (E2) with laying rate in two different breeds of egg-type chickens, i.e. White Leghorn (WL) and Greenleg Partridge (GP). Thirty-five-week-old WL (line H22; n = 8) and GP (line Z11; n = 8) hens were used in the experiment. Blood samples were collected from hens at the time of C1 oviposition (i.e. 0.5 h before C2 ovulation), then at 20, 16, 12, 8, 4, 2 h before C3 ovulation, and at the time of predicted C3 ovulation (i.e. just after C2 oviposition). On the following day, six birds of each breed were decapitated 2 h before ovulation on the next day after the last blood collection. A stroma, small (SWF; 1-4 mm), medium (MWF; 4-6 mm) and large (LWF; 6-8 mm) white prehierarchical follicles, and yellow hierarchical (F6-F1; 8-36 mm) ovarian follicles were isolated. P4 and E2 levels in blood plasma and ovarian follicles were determined radioimmunologically. The average rate of lay in WL hens was significantly higher than in GP hens (P<0.05). The highest concentrations of P4 and E2 were found 4 h before ovulation in both WL and GP hens. In WL hens the levels of P4 at the time of oviposition and at 4 and 2 h before ovulation were significantly higher in comparison with GP hens (P<0.05). On the other hand, higher E2 concentrations were found in GP hens at the time of oviposition and 12, 8 and 4 h before ovulation (P<0.05). In LWF and hierarchical follicles of WL hens the level of P4 was significantly lower than in GP hens (P<0.05). With respect to E2, a reverse relationship was observed in LWF and F6-F4 follicles. In conclusion, the results obtained indicate that in egg-type layers the profiles of plasma and ovarian sex steroids depend on their genotype. The significant differences in sex steroid levels in blood and ovarian follicles of the WL and GP breeds may contribute to explain the endocrinological factors that determined their laying performance and productivity.

scholarly journals Microfluidics for minute DNA sample analysis: open challenges for genetic testing of cell-free circulating DNA in blood plasma

2018 ◽  
Vol 1 ◽  
pp. 25-32 ◽  
Author(s):  
Rémi Malbec ◽  
Jean Cacheux ◽  
Pierre Cordelier ◽  
Thierry Leichlé ◽  
Pierre Joseph ◽  
...  
Keyword(s):  
Genetic Testing ◽  
Blood Plasma ◽  
Circulating Dna ◽  
Sample Analysis ◽  
Free Circulating Dna ◽  
In Blood Plasma

scholarly journals The possibility of using freely circulating DNA blood plasma in preoperative diagnosis of thyroid tumors

2020 ◽  
Author(s):  
Vera Kachko ◽  
Vladimir Vanushko ◽  
Nadezhda Platonova ◽  
Aleksandr Abrosimov ◽  
Galina Snigireva ◽  
...  
Keyword(s):  
Thyroid Cancer ◽  
Blood Plasma ◽  
Hot Spots ◽  
Somatic Mutations ◽  
Gene Mutations ◽  
Circulating Dna ◽  
Thyroid Tumors ◽  
Braf Gene ◽  
Gene Exon ◽  
In Blood Plasma

Background: The feasibility of using molecular genetic markers for the diagnosis of thyroid tumors and the impact on the prognosis of thyroid cancer are being actively investigated. The most interesting are genes, the detection of which is associated not only with thyroid cancer, but also with a more aggressive course of the disease. The ability to diagnose the molecular profile of minimally invasive methods with the study of freely circulating DNA tumor tissue in blood plasma is a modern trend of medicine. Aims: to evaluate the frequency of somatic mutations in the "hot spots" of BRAF, KRAS, KRAS, EIF1AX and TERT genes in circulating DNA of blood plasma. Materials and methods: Samples of DNA, extracted from the removed tumor and non-tumor thyroid tissue, were tested for the presence of somatic mutations in hot spots of the genes BRAF, KRAS, NRAS, TERT, and EIF1AX and then in identifying mutations and testing appropriate samples of free circulating DNA in blood plasma. Results: mutations in the" hot spots "of the BRAF gene (exon 15, codon area 600-601) were found in 54 patients, mutations in the" hot spots " of the NRAS gene (exon 3, codon 61) in 12 patients; mutations in the hot spots of the KRAS, TERT and EIF1AX genes were not detected. In freely circulating blood plasma DNA, BRAF gene mutations were detected in 1 case, NRAS gene mutations were detected in 1 case. Conclusions: the use of freely circulating DNA of blood plasma in the testing of the studied sample did not show the feasibility for the diagnosis of thyroid tumors.

scholarly journals Direct characterization of circulating DNA in blood plasma using μLAS technology

2017 ◽  
Author(s):  
R. Malbec ◽  
B. Chami ◽  
H. H. T. Ngo ◽  
A. Didelot ◽  
F. Garlan ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Circulating Dna ◽  
In Blood Plasma

The Origin of Elevated Levels of Circulating DNA in Blood Plasma of Premature Neonates

2008 ◽  
Vol 1137 (1) ◽  
pp. 27-30 ◽  
Author(s):  
N. O. Tuaeva ◽  
Z. I. Abramova ◽  
V. V. Sofronov
Keyword(s):  
Blood Plasma ◽  
Circulating Dna ◽  
Premature Neonates ◽  
In Blood Plasma

State of hormonal regulation in experimental animals exposed to noise and vibration

2020 ◽  
pp. 680-681
Author(s):  
A. V. Lizarev ◽  
V. A. Pankov
Keyword(s):  
Blood Plasma ◽  
Hormonal Regulation ◽  
Adrenocorticotropic Hormone ◽  
Vibration Exposure ◽  
Noise And Vibration ◽  
Experimental Animals ◽  
In Blood Plasma

When exposed to noise and vibration in experimental animals there was a decrease in the content of threeiodinethyronine, thyroxin and adrenocorticotropic hormone in blood plasma after 15 and 30 days of experience. An increase in loads led to an increase in the level of threeiodinethyronine and thyroxin under vibration exposure and was normalized with noise. The content of adrenocorticotropic hormone leveled in both cases.

EFFECT OF DIETARY LEVELS OF SODIUM AND POTASSIUM ON GROWTH AND ON CONCENTRATIONS IN BLOOD PLASMA AND TISSUES OF WHITE RAT

1950 ◽  
Vol 162 (1) ◽  
pp. 182-188 ◽  
Author(s):  
J. H. Meyer ◽  
R. R. Grunert ◽  
Marie T. Zepplin ◽  
R. H. Grummer ◽  
G. Bohstedt ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Sodium And Potassium ◽  
In Blood Plasma
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