Mapping quantitative trait loci associated with yield and its related traits in Sorghum bicolor

Abstract We describe a recombinant inbred line (RIL) population of 161 F5 genotypes for the widest euploid cross that can be made to cultivated sorghum (Sorghum bicolor) using conventional techniques, S. bicolor × Sorghum propinquum, that segregates for many traits related to plant architecture, growth and development, reproduction, and life history. The genetic map of the S. bicolor × S. propinquum RILs contains 141 loci on 10 linkage groups collectively spanning 773.1 cM. Although the genetic map has DNA marker density well-suited to quantitative trait loci mapping and samples most of the genome, our previous observations that sorghum pericentromeric heterochromatin is recalcitrant to recombination is highlighted by the finding that the vast majority of recombination in sorghum is concentrated in small regions of euchromatin that are distal to most chromosomes. The advancement of the RIL population in an environment to which the S. bicolor parent was well adapted (indeed bred for) but the S. propinquum parent was not largely eliminated an allele for short-day flowering that confounded many other traits, for example, permitting us to map new quantitative trait loci for flowering that previously eluded detection. Additional recombination that has accrued in the development of this RIL population also may have improved resolution of apices of heterozygote excess, accounting for their greater abundance in the F5 than the F2 generation. The S. bicolor × S. propinquum RIL population offers advantages over early-generation populations that will shed new light on genetic, environmental, and physiological/biochemical factors that regulate plant growth and development.

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Whole‐genome resequencing and transcriptome analysis provide insights on aphid‐resistant quantitative trait loci/genes in Sorghum bicolor

Plant Breeding ◽

10.1111/pbr.12946 ◽

2021 ◽

Author(s):

Jiwei Zhang ◽

Wanglong Li ◽

Peng Lv ◽

Xin Yang ◽

Wang Xu ◽

...

Keyword(s):

Quantitative Trait Loci ◽

Sorghum Bicolor ◽

Transcriptome Analysis ◽

Quantitative Trait ◽

Whole Genome ◽

Genome Resequencing ◽

Trait Loci ◽

Whole Genome Resequencing

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Improving Drought Tolerance in Sorghum bicolor L. Moench: Marker-Assisted Transfer of the Stay-Green Quantitative Trait Loci (QTL) from a Characterized Donor Source into a Local Farmer Variety

International Journal of Scientific Research in Knowledge ◽

10.12983/ijsrk-2013-p154-162 ◽

2013 ◽

Vol 1 (6) ◽

pp. 154-162 ◽

Cited By ~ 5

Author(s):

Kahiu Ngugi ◽

Wilson Kimani ◽

Dan Kiambi ◽

Eunice W. Mutitu

Keyword(s):

Quantitative Trait Loci ◽

Drought Tolerance ◽

Sorghum Bicolor ◽

Quantitative Trait ◽

Stay Green ◽

Trait Loci ◽

Local Farmer ◽

Sorghum Bicolor L ◽

Donor Source

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Inclusive composite-interval mapping reveals quantitative trait loci for plant architectural traits in sorghum (Sorghum bicolor)

Crop and Pasture Science ◽

10.1071/cp18408 ◽

2019 ◽

Vol 70 (8) ◽

pp. 659

Author(s):

Huawen Zhang ◽

Runfeng Wang ◽

Bin Liu ◽

Erying Chen ◽

Yanbing Yang ◽

...

Keyword(s):

Quantitative Trait Loci ◽

Sorghum Bicolor ◽

Quantitative Trait ◽

Composite Interval Mapping ◽

Recombinant Inbred Lines ◽

Interval Mapping ◽

Environment Interaction ◽

Trait Loci ◽

Inclusive Composite Interval Mapping ◽

Architectural Traits

Architecture-efficient sorghum (Sorghum bicolor (L.) Moench) has erect leaves forming a compact canopy that enables highly effective utilisation of solar radiation; it is suitable for high-density planting, resulting in an elevated overall production. Development of sorghum ideotypes with optimal plant architecture requires knowledge of the genetic basis of plant architectural traits. The present study investigated seven production-related architectural traits by using 181 sorghum recombinant inbred lines (RILs) with contrasting architectural phenotypes developed from the cross Shihong 137 × L-Tian. Parents along with RILs were phenotyped for plant architectural traits for two consecutive years (2012, 2013) at two locations in the field. Analysis of variance revealed significant (P ≤ 0.05) differences among RILs for architectural traits. All traits showed medium to high broad-sense heritability estimates (0.43–0.94) and significant (P ≤ 0.05) genotype × environment effects. We employed 181 simple sequence repeat markers to identify quantitative trait loci (QTLs) and the effects of QTL × environment interaction based on the inclusive composite interval mapping algorithm. In total, 53 robust QTLs (log of odds ≥4.68) were detected for these seven traits and explained 2.11–12.11% of phenotypic variation. These QTLs had small effects of QTL × environment interaction and yet significant epistatic effects, indicating that they could stably express across environments but influence phenotypes through strong interaction with non-allelic loci. The QTLs and linked markers need to be verified through function and candidate-gene analyses. The new knowledge of the genetic regulation of architectural traits in the present study will provide a theoretical basis for the genetic improvement of architectural traits in sorghum.

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Quantitative trait loci analysis of economically important traits in Sorghum bicolor × S. sudanense hybrid

Canadian Journal of Plant Science ◽

10.4141/cjps09112 ◽

2011 ◽

Vol 91 (1) ◽

pp. 81-90 ◽

Cited By ~ 9

Author(s):

Lu Xiao-ping ◽

Yun Jin-feng ◽

Gao Cui-ping ◽

Surya Acharya

Keyword(s):

Quantitative Trait Loci ◽

Sorghum Bicolor ◽

Quantitative Trait ◽

Agronomic Traits ◽

Random Amplified Polymorphic Dna ◽

Female Parent ◽

Leaf Length ◽

Linkage Groups ◽

Plant Weight ◽

Trait Loci

Lu, X-p., Yun, J-f., Gao, C-p. and Acharya, S. 2011. Quantitative trait loci analysis of economically important traits in Sorghum bicolor×S. sudanense hybrid. Can. J. Plant Sci. 91: 81–90. Many agronomic traits of Sorghum bicolor×S. sudanense hybrid are quantitatively inherited, and the gene mapping of these traits has important research and practical consequences. In this study, genetic mapping and quantitative trait loci (QTL) analyses were conducted using 248 F2:3 plants of a cross between sorghum 314A (female parent) and Sudan grass 2002GZ-1 (male parent). A total of 178 markers (170 amplified fragment length polymorphism and 8 random amplified polymorphic DNA) were employed to construct a linkage map with 10 linkage groups covering 836 cM of the genome. The two parents expressed polymorphism for 10 agronomic characters (plant height, stem diameter, leaf number, leaf length, leaf width, spike length, tiller number, ratio of stem and leaf weight, fresh plant weight and dry plant weight). When analyzed for possible QTLs a total of 98 QTLs were identified in two test sites, out of which 26 QTLs overlapped in both sites. The average number of QTLs per character was found to be 2.6 and the distributions of these QTLs were found to be uneven across linkage groups. This, and the fact that molecular marker densities were not proportional with QTL frequencies, indicates that the detectable QTLs correlated with the agronomic traits and the genetic map can be useful for improvement in relevant characters in Sorghum bicolor×S. sudanense hybrids.

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