scholarly journals Detection of Shiga toxin produced by Escherichia coli in poultry and meat in Luxor city using multiplex PCR

2016 ◽  
Vol 31 (2) ◽  
pp. 40-44
Author(s):  
Jehan Daoud ◽  
Karmi Mohamed ◽  
Soad Nasef ◽  
Reham Ahmed
Keyword(s):  
Escherichia Coli ◽  
Multiplex Pcr ◽  
Shiga Toxin

Multiplex PCR method for the detection of human norovirus, Salmonella spp., Shigella spp., and shiga toxin producing Escherichia coli in blackberry, coriander, lettuce and strawberry

2021 ◽  
pp. 103926
Author(s):  
Omar Hernández Hernández ◽  
Ana L. Gutiérrez-Escolano ◽  
Cleo Cancio-Lonches ◽  
Montserrat H. Iturriaga ◽  
Juan Ramiro Pacheco-Aguilar ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multiplex Pcr ◽  
Shiga Toxin ◽  
Salmonella Spp ◽  
Human Norovirus ◽  
Shigella Spp ◽  
Pcr Method

scholarly journals Real-Time Multiplex PCR for Detecting Shiga Toxin 2-Producing Escherichia coli O104:H4 in Human Stools

2012 ◽  
Vol 50 (5) ◽  
pp. 1752-1754 ◽  
Author(s):  
W. Zhang ◽  
M. Bielaszewska ◽  
A. Bauwens ◽  
A. Fruth ◽  
A. Mellmann ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multiplex Pcr ◽  
Real Time ◽  
Shiga Toxin ◽  
Shiga Toxin 2

scholarly journals Identification of Novel Biomarkers for Priority Serotypes of Shiga Toxin-Producing Escherichia coli and the Development of Multiplex PCR for Their Detection

2018 ◽  
Vol 9 ◽  
Author(s):  
Matthias Kiel ◽  
Pierre Sagory-Zalkind ◽  
Céline Miganeh ◽  
Christoph Stork ◽  
Andreas Leimbach ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multiplex Pcr ◽  
Shiga Toxin ◽  
Novel Biomarkers

scholarly journals Evaluating the efficacy of beef slaughter line interventions by quantifying the six major non-O157 Shiga toxin producing Escherichia coli serogroups using real-time multiplex PCR

2017 ◽  
Vol 63 ◽  
pp. 228-238 ◽  
Author(s):  
Kaushi S.T. Kanankege ◽  
Kelly S. Anklam ◽  
Catherine M. Fick ◽  
Megan J. Kulow ◽  
Charles W. Kaspar ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multiplex Pcr ◽  
Real Time ◽  
Shiga Toxin

Multiplex PCR Detection of Shiga Toxin–Producing Escherichia coli Strains Belonging to Serogroups O157, O103, O91, O113, O145, O111, and O26 Experimentally Inoculated in Beef Carcass Swabs, Beef Trim, and Ground Beef

2011 ◽  
Vol 74 (2) ◽  
pp. 228-239 ◽  
Author(s):  
ANGELA M. VALADEZ ◽  
CHITRITA DEBROY ◽  
EDWARD DUDLEY ◽  
CATHERINE N. CUTTER
Keyword(s):  
Escherichia Coli ◽  
Multiplex Pcr ◽  
Shiga Toxin ◽  
Gastrointestinal Disease ◽  
Simultaneous Detection ◽  
Ground Beef ◽  
Multiplex Pcr Assay ◽  
Significant Difference ◽  
Beef Products ◽  
Beef Carcass

Numerous foodborne outbreaks are attributed to Shiga toxin–producing Escherichia coli (STEC) and have been recognized for causing gastrointestinal disease in humans. Beef products have been considered the principal source of STEC. A multiplex PCR assay enabling simultaneous detection of STEC O103, O91, O113, O145, O111, O157, and O26 was developed and evaluated in artificially contaminated beef carcass swabs, beef trim, and ground beef after overnight enrichment. Individual serogroups were experimentally inoculated at low (1 to 10 CFU/ml) and high (11 to 100 CFU/ml) levels, and with a cocktail of strains belonging to two, four, and six serogroups. There was no significant difference in detecting single STEC strains under the different conditions. Only when strains were combined were there significant differences in detection of all cocktail isolates in some of the beef products. To address this issue, four serogroups were experimentally inoculated together at three different estimated levels (10, 102, and 103 CFU/ml) in all three beef products. Results yielded no significant difference in detecting STEC at the three inoculation levels (10, 102, and 103 CFU/ml) in trim and carcass swabs, but there was a significant difference in detecting STEC at the lowest levels (10 and 102 CFU/ml) in the 80:20 nonirradiated ground beef, and in the detection of STECin irradiated ground beef. The findings from this study could provide industry and government agencies with a tool to evaluate the prevalence and incidence of STEC in beef products and their processing environments.

scholarly journals Detection of five Shiga toxin-producing Escherichia coli genes with multiplex PCR

2014 ◽  
Vol 40 ◽  
pp. 31-40 ◽  
Author(s):  
Insook Son ◽  
Rachel Binet ◽  
Anna Maounounen-Laasri ◽  
Andrew Lin ◽  
Thomas S. Hammack ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multiplex Pcr ◽  
Shiga Toxin
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