scholarly journals Antimicrobial susceptibility of Staphylococcus aureus clinical isolates and prevalence of MRSA in ICUs of Mansoura University Hospitals

2016 ◽  
Vol 25 (2) ◽  
pp. 93-97
Author(s):  
Eman El Gemezy ◽  
Fathy Serry ◽  
Ashraf Kadry
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Susceptibility ◽  
Clinical Isolates ◽  
University Hospitals

scholarly journals 467. Antimicrobial Resistance and Molecular Analysis of Staphylococcus aureus in Staphylococcal Scalded Skin Syndrome among Children in Korea

2019 ◽  
Vol 6 (Supplement_2) ◽  
pp. S229-S229
Author(s):  
Jae Hong Choi ◽  
Hyunju Lee ◽  
Eun Hwa Choi
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Susceptibility ◽  
Molecular Characteristics ◽  
University Hospitals ◽  
Korean Children ◽  
Methicillin Resistant ◽  
Staphylococcal Scalded Skin Syndrome ◽  
High Prevalence ◽  
The Mean ◽  
Susceptibility Patterns

Abstract Background Staphylococcal scalded skin syndrome (SSSS) is a blistering and desquamative skin disease caused by the exfoliative toxins of Staphylococcus aureus. SSSS mainly affects children younger than 5 years of age. Although many countries show a predominance of methicillin-susceptible S. aureus (MSSA), recently an increase in cases due to methicillin-resistant S. aureus (MRSA) has been reported. We investigated the molecular characteristics of S. aureus isolated from the children with SSSS in Korea. Methods From January 2010 to December 2017, children clinically diagnosed as SSSS under the age of 5 years were enrolled. Cases from 3 different university hospitals in Korea were included. S. aureus isolated from nasal, axillary, or inguinal area of the children were analyzed for multilocus sequence type and exfoliative toxins (eta, etb). Medical records were retrospectively reviewed for clinical characteristics and antimicrobial susceptibility patterns of S. aureus. Results A total of 26 cases were enrolled. The mean age was 2.3 years (range, 0–4.8 years). Twenty-two (84.6%) patients were hospitalized. Skin manifestations were classified as follows; generalized (n = 10, 38.5%), intermediate (n = 11, 42.3%), and abortive (n = 5, 19.2%). Twenty-five isolates (96.2%) were resistant to methicillin and macrolide-resistance was found in 92.3% (n = 24). ST89 (n = 21, 80.8%) was the most prevalent clone, with single clones of ST1, ST5, ST72, ST121, and ST1507. The eta gene was detected in 1 (3.8%) MSSA isolate. The etb gene was detected in 14 (53.8%) isolates all of which were ST89. All patients were treated with antibiotics, and the mean duration was 8.3 days regardless of the administration route. Nafcillin or first cephalosporin was most commonly prescribed (n = 20, 76.9%), clindamycin was administered in combination in 9 patients (34.6%) and vancomycin in 4 patients (15.4%). Among the 25 MRSA cases, only 6 (24.0%) were treated with susceptible antibiotics. However, there was no difference in treatment duration according to antimicrobial susceptibility (8.43:8.22 days, P > 0.05). Conclusion The molecular epidemiology of S. aureus isolated from the Korean children with SSSS demonstrated the high prevalence of methicillin-resistant ST89 clone that harbors the etb gene. Disclosures All authors: No reported disclosures.

Clinical relevance and antimicrobial susceptibility profile of the unknown human pathogen Corynebacterium aurimucosum

2021 ◽  
Vol 70 (3) ◽  
Author(s):  
Charles R. Lefèvre ◽  
Romain Pelletier ◽  
Alban Le Monnier ◽  
Stéphane Corvec ◽  
Emmanuelle Bille ◽  
...  
Keyword(s):  
Antimicrobial Susceptibility ◽  
Clinical Relevance ◽  
Type Species ◽  
Clinical Isolates ◽  
Penicillin G ◽  
University Hospitals ◽  
Content Type ◽  
Link Type ◽  
Antimicrobial Susceptibility Profile ◽  
Tract Infections

Introduction. Even though Corynebacterium aurimucosum has been described in 2002, this species has long been underestimated due to the unreliability of conventional identification methods and only a few cases of infections have been reported. Hypothesis/Gap Statement. Little is known about clinical significance and antimicrobial susceptibility profile of this uncommon species. Aim. To evaluate the clinical relevance of C. aurimucosum and its antimicrobial susceptibility profile. Methodology. All C. aurimucosum isolates, collected from 2010 to 2019 in 10 French university hospitals, were retrospectively included. Demographic, clinical and microbiological data were collected for all cases. Antimicrobial susceptibility testing was performed according to the 2019 EUCAST guidelines. Results. Fifty-seven clinical isolates of C. aurimucosum were collected in 57 patients (median age, 65.8 years; male/female sex ratio, 1.1), mostly from urine (28 %), blood culture (28 %) and bone/synovial fluid (19 %) samples. Of them, 14 cases of infection were confirmed, mainly bone and joint infections (50 %) followed by urinary tract infections (UTIs) (21 %), bacteremia (14 %), skin and soft-tissue infections (14 %). C. aurimucosum was recovered in pure culture in 36 % of cases (UTIs and bacteremia) while mixed cultures were observed for other infections. By testing 52 clinical isolates in vitro, this species appeared to be fully susceptible to linezolid and vancomycin while most isolates (>80 %) were susceptible to amoxicillin (MIC90, 2 µg ml−1), gentamicin, tetracycline and rifampicin. Both cefotaxime and ciprofloxacin seemed to have a limited activity (ca. 50 % of susceptible strains). The MIC distribution for ciprofloxacin showed a bimodal profile with a population of highly-resistant strains with MICs >2 µg ml−1. Most isolates (>90 %) were categorized as resistant to penicillin G and clindamycin. Conclusion. C. aurimucosum should be considered as an actual opportunistic pathogen, and treatment with amoxicillin, vancomycin or linezolid should be preferred.

scholarly journals Antimicrobial Susceptibility Trends among Staphylococcus aureus Isolates from U.S. Hospitals: Results from 7 Years of the Ceftaroline (AWARE) Surveillance Program, 2010 to 2016

2017 ◽  
Vol 61 (9) ◽  
Author(s):  
Helio S. Sader ◽  
Rodrigo E. Mendes ◽  
Jennifer M. Streit ◽  
Robert K. Flamm
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Susceptibility ◽  
Clinical Isolates ◽  
Surveillance Program ◽  
Broth Microdilution ◽  
Methicillin Resistant ◽  
Content Type ◽  
Medical Centers

ABSTRACT We evaluated trends in Staphylococcus aureus antimicrobial susceptibility in U.S. hospitals in the 2010–2016 period. A total of 21,056 clinical isolates from 42 medical centers were tested for susceptibility by broth microdilution methods. Methicillin-resistant S. aureus (MRSA) rates decreased from 50.0% (in 2010) to 42.2% (in 2016). Susceptibility to erythromycin, levofloxacin, and clindamycin increased slightly, whereas susceptibility to ceftaroline, trimethoprim-sulfamethoxazole, and tetracycline remained stable. Ceftaroline retained potent activity against methicillin-susceptible S. aureus (MSSA) and MRSA (97.2% susceptible) with no marked variations.

scholarly journals Antimicrobial susceptibility patterns of clinical isolates of Staphylococcus aureus in Eastern Nepal

1970 ◽  
Vol 9 (2) ◽  
pp. 78-82 ◽  
Author(s):  
R Baral ◽  
B Khanal ◽  
A Acharya
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Susceptibility ◽  
Wide Spectrum ◽  
Response To Therapy ◽  
Clinical Isolates ◽  
Current Status ◽  
Diffusion Method ◽  
Isolation And Identification ◽  
Clinical Specimens ◽  
Set Up

Background: Staphylococcus aureus, versatile pathogen causes many serious and life threatening infections. Resistant S aureus has become a serious matter of concern. The antimicrobial susceptibility profile of local isolates is essential for the selection of appropriate therapy for the management of staphylococcal infections. Objective: To find out the current status of antimicrobial resistance among the clinical isolates in our set up. Method: S aureus isolated from the clinical specimens submitted to the microbiology unit of clinical laboratory services, BP Koirala Institute of Health Sciences (BPKIHS) hospital were studied. Isolation and identification of S aureus was done by standard microbiological technique. Results: A total 300 S aureus isolates were obtained from various clinical specimens. S aureus showed susceptible to chlorampenicol (95%), tetracycline (94.3%), cefotaxime (93.3%), erythromycin (89%), ciprofloxacin (88.35%) and gentamicin (78%).Sixty four percent of isolates were found to be resistant to co-trimoxazole and 26% were methicillin resistant (MRSA). Nearly half (52.66%) of S aureus showed resistance to penicillin. All the isolates were susceptible to vancomycin in disc diffusion method. It was isolated frequently from pus 223(74%) followed by blood (14%). Forty eight percent of isolates were from abscess, followed by sepsis (17%). Conclusion: Resistant S aureus is a common pathogen causing a wide spectrum of infections in our set up. Existence of MRSA among local isolates is a serious matter of concern. Although no isolate exhibited resistance to vancomycin, screening test and MIC determination are recommended in monitoring the response to therapy and for early detection of impeding resistance among local strains. Keywords: staphylococcus aureus; antimicrobial susceptibility; BPKIHS DOI: http://dx.doi.org/10.3126/hren.v9i2.4977 Health Renaissance 2011: Vol.9 (No.2): 78-82

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