Effects of temperature on biodegradation characteristics of organic pollutants and microbial community in a solid phase aerobic bioreactor treating high strength organic wastewater

2001 ◽  
Vol 43 (1) ◽  
pp. 131-137 ◽  
Author(s):  
B.-R. Lim ◽  
X. Huang ◽  
H.-Y. Hu ◽  
N. Goto ◽  
K. Fujie
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Microbial Community Structure ◽  
Solid Phase ◽  
Removal Rate ◽  
High Strength ◽  
Quinone Profile ◽  
Higher Temperature ◽  
Bod Removal ◽  
Organic Wastewater

The BOD removal rate and microbial community structure in a solid phase aerobic bioreactor using polyvinyl alcohol gel particles as packing material for the treatment of high strength organic wastewater were investigated at various temperatures. The BOD removal rate in the bioreactor increased when the temperature increased from 20°C to 30°C, 40°C, and 50°C, but it decreased when the temperature increased from 50°C to 60°C. Higher temperature enhanced the endogenous respiration of microbes in the bioreactor. The microbial community structure in the bioreactor was analyzed with quinone profile. The experimental results showed that the microbial community structure in the bioreactor was significantly affected by temperature. The dominant quinone of the microbes inhabiting the bioreactor was ubiquinone-8 at 30°C, but that at 50°C and 60°C was menaquinone-7. It was estimated that the thermophilic Bacillus having menaquinone-7 dominated in the bioreactor at higher temperature. The microbial diversity in the bioreactor varied with temperature.

Analysis of Microbial Community Structure and Volatile Compounds in the Pit Mud Used for Manufacturing Taorong-type Baijiu Based on High-Throughput Sequencing

2021 ◽  
Author(s):  
Yanbo Liu ◽  
Mengxiao Sun ◽  
Pei Hou ◽  
Wenya Wang ◽  
Xiangkun Shen ◽  
...  
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Qualitative Analysis ◽  
Volatile Compounds ◽  
High Throughput ◽  
Microbial Community Structure ◽  
High Throughput Sequencing ◽  
Solid Phase ◽  
Gas Chromatography Mass Spectrometry ◽  
Pit Mud

Abstract In this study, the pit mud used in manufacturing Taorong-type Baijiu was collected from the upper, middle, lower and bottom layers of pits in Henan Yangshao Liquor Co., LTD. Besides, high-throughput sequencing (HTS) technology was adopted to analyze the microbial community structure of the pit mud. In addition, the volatile compounds in the pit mud were subjected to preliminarily qualitative analysis through headspace-solid phase microextraction (HS-SPME) and gas chromatography-mass spectrometry (GC-MS). The results of HTS demonstrated that there were 5, 3, 5 and 5 dominant bacterial phyla (including 11, 11, 9 and 8 dominant bacterial genera) and 3, 3, 3 and 3 dominant fungal phyla (including 4, 7, 7 and 5 dominant fungal genera) in the pit mud from F-S (upper), G-Z (middle), H-X (lower) and I-D (bottom), respectively. The qualitative analysis results of volatile compounds demonstrated that a total of 78 kinds of volatile compounds were detected in the pit mud, including 46, 45, 39 and 49 kinds in the pit mud from F-S, G-Z, H-X and I-D, respectively. Ester and acid were the two main components in the pit mud. Meanwhile, the correlation between microorganisms and main volatile compounds in the pit mud was analyzed. Moreover, Lentimicrobium, Syner-01 and Blvii28_wastewater-sludge group were found for the first time in the pit mud used for manufacturing Taorong-type Baijiu. The findings of this study could provide a theoretical foundation for improving the quality of pit mud and the flavor of Taorong-type Baijiu.

Change of microbial community structure by respiratory quinone profile at intermittently aerated membrane bioreactor

2004 ◽  
Vol 49 (5-6) ◽  
pp. 459-465 ◽  
Author(s):  
P. Songprasert ◽  
B.-R. Lim ◽  
K.-H. Ahn
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Microbial Community Structure ◽  
Membrane Bioreactor ◽  
Domestic Wastewater ◽  
Time Interval ◽  
Respiratory Quinone ◽  
Aerobic Conditions ◽  
Submerged Membrane Bioreactor ◽  
Quinone Profile

In the present study, the microbial community structure in an intermittently aerated submerged membrane bioreactor treating domestic wastewater was observed using the respiratory quinone profiles. The effects of different time interval for the aerobic and anoxic period on microbial community structure were examined with 60/90 min (Step 1) and 90/60 min (Step 2) as anoxic/aerobic periods. There was an observable slight difference in microbial community structure between Step 1 and Step 2 in the submerged membrane bioreactor. The dominant quinone types for Step 1 at both anoxic and aerobic conditions were UQ-8 followed by UQ-10 and MK-6, but those for Step 2 were UQ-8, MK-6 and MK-10(H4). The microbial diversity of Step 1 and Step 2 based on the composition of all quinones was 10.6Ð11.7 and 13.3Ð13.0 for anoxic and aerobic conditions, respectively. The present results suggest that the introduction of intermittent aeration into the submerged membrane bioreactor has little influence on the bacterial community structure.

scholarly journals Flavor Composition and Microbial Community Structure of Mianning Ham

2021 ◽  
Vol 11 ◽  
Author(s):  
Lin Chen ◽  
Zhengli Wang ◽  
Lili Ji ◽  
Jiamin Zhang ◽  
Zhiping Zhao ◽  
...  
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
High Throughput ◽  
Microbial Community Structure ◽  
High Throughput Sequencing ◽  
Solid Phase ◽  
Debaryomyces Hansenii ◽  
Culture Method ◽  
Quality Parameters ◽  
Staphylococcus Equorum

Mianning ham, a traditional Chinese dry-cured ham, is protected by national geographical indications. To understand the surface and internal flavor composition and microbial community structure of Mianning ham, solid phase microextraction-gas chromatography (SPME-GC-MS) technology and Illumina high-throughput sequencing were utilized. The results showed that a total of 60 flavor substances were identified in the hams. Forty-nine kinds of flavorings were identified on the surface, including 14 aldehydes, 6 ketones, 10 alcohols, 5 esters, 7 hydrocarbons, 5 acids, and 2 other compounds. Thirty-six kinds of internal flavorings were identified, including 13 aldehydes, 4 ketones, 6 alcohols, 3 esters, 5 hydrocarbons, 4 acids and 1 other type. Decanal (34.91 μg/g) was the most prevalent compound on the surface, followed by n-hexanol (24.99 μg/g), n-hexanal (20.20 μg/g), and n-octyl (16.14 μg/g). n-Hexanal (20.74 μg/g) was the most common compound internally, followed by non-aldehyde (5.70 μg/g), 1-octene-3-alcohol (3.54 μg/g), and inverse-2-octenal (2.77 μg/g). Penicillium lanosum, Penicillium nalgiovense, Debaryomyces hansenii, Staphylococcus equorum, and Erwinia tasmaniensis were isolated from the surfaces of the hams by the traditional culture method. By Illumina high-throughput sequencing, three fungal phyla were identified. Ascomycota was the dominant phylum followed by Basidiomycota. At the genus level, 11 fungi were identified, of which Aspergillus was the dominant fungus, followed by Penicillium and Wallemia. These findings provide fundamental knowledge regarding the microorganisms and flavor compounds in Mianning ham, which will help industrial processors develop effective strategies for standardizing quality parameters.

Changes in the microbial community structure during thermophilic composting of manure as detected by the quinone profile method

2004 ◽  
Vol 39 (12) ◽  
pp. 1999-2006 ◽  
Author(s):  
Jing-Chun Tang ◽  
Tomonari Kanamori ◽  
Yasushi Inoue ◽  
Tsuyoshi Yasuta ◽  
Shigekata Yoshida ◽  
...  
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Microbial Community Structure ◽  
Profile Method ◽  
Quinone Profile

The First Use of Biofilm Microbial Community Structure as an Indicator of Impact of Two Dams on the Elwha River (Washington)

2009 ◽  
Vol 27 (4) ◽  
pp. 385-387
Author(s):  
W. D. Eaton ◽  
B. Wilmot ◽  
E. Epler ◽  
S. Mangiamelli ◽  
D. Barry
Keyword(s):  
Community Structure ◽  
Microbial Community ◽  
Microbial Community Structure ◽  
Elwha River
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