Production of Rhamnolipid Biosurfactant from Fed Batch Culture by Pseudomonas aeruginosa using Multiple Substrates

2020 ◽  
Vol 16 (6) ◽  
pp. 928-933
Author(s):  
Jujjavarapu S. Eswari
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Batch Process ◽  
Time Interval ◽  
Fed Batch ◽  
Multiple Substrates ◽  
Surface Active Agents ◽  
Limiting Nutrients ◽  
Limiting Nutrient ◽  
Surface Active ◽  
Rhamnolipid Biosurfactant

Objective: Biosurfactants are the surface active agents which are used for the reduction of surface and interfacial tensions of liquids. Rhamnolipids are the surfactants produced by Pseudomonas aeruginosa. It requires minimum nutrition for its growth as it can also grow in distilled water. The rhamnolipids produced by Pseudomonas aeruginosa are extra-cellular glycolipids consisting of L-rhamnose and 3-hydroxyalkanoic acid. Methods: The fed-batch method for the rhamnolipid production is considered in this study to know the influence of the carbon, nitrogen, phosphorous substrates as growth-limiting nutrients. Pulse feeding is employed for limiting nutrient addition at particular time interval to obtain maximum rhamnolipid formation from Pseudomonas aeruginosa compared with the batch process. Results: Out of 3 fed batch strategies constant glucose fed batch strategy shows best and gave maximum rhamnolipid concentration of 0.134 g/l.

scholarly journals Strategies for improved rhamnolipid production by Pseudomonas aeruginosa PA1

2015 ◽  
Author(s):  
Alexandre Soares dos Santos ◽  
Nei Pereira Jr ◽  
Denise M G Freire
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Stationary Phase ◽  
Culture Medium ◽  
Fold Increase ◽  
Batch Process ◽  
Measurable Effect ◽  
Fed Batch ◽  
Batch Operation ◽  
Ph Values ◽  
Influence Of Ph

Rhamnolipids are biosurfactants with potential for diversified industrial and environmental uses. The present study evaluated three strategies to increase the production of rhamnolipid-type biosurfactants produced by Pseudomonas aeruginosa strain PA1. The influence of pH, the addition of endogenous autoinducers and the use of a fed batch process were examined. The culture medium adjusted to pH 7.0 was the most productive. Furthermore, the pH of the culture medium had a measurable effect on the ratio of mono- and dirhamnolipids synthesized. At pH values below 7.3, the proportion of monorhamnolipids decreased from 45 to 24%. Additionally, recycling 20% of the spent culture medium where P. aeruginosa was grown up to the later stationary phase was responsible for a 100% increase in rhamnolipid volumetric productivity in the new culture medium. Finally, the use of fed batch operation under conditions of limited nitrogen resulted in a 3.8-fold increase in the amount of rhamnolipids produced (1.29 g L-1 to 4.90 g L-1, as rhamnose). These results offer promising paths to optimize processes for the production of rhamnolipids.

scholarly journals Strategies for improved rhamnolipid production byPseudomonas aeruginosaPA1

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e2078 ◽  
Author(s):  
Alexandre Soares dos Santos ◽  
Nei Pereira Jr ◽  
Denise M.G. Freire
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Stationary Phase ◽  
Culture Medium ◽  
Fold Increase ◽  
Batch Process ◽  
Measurable Effect ◽  
Fed Batch ◽  
Batch Operation ◽  
Ph Values ◽  
Influence Of Ph

Rhamnolipids are biosurfactants with potential for diversified industrial and environmental uses. The present study evaluated three strategies for increasing the production of rhamnolipid-type biosurfactants produced byPseudomonas aeruginosastrain PA1. The influence of pH, the addition ofP. aeruginosaspent culture medium and the use of a fed-batch process were examined. The culture medium adjusted to pH 7.0 was the most productive. Furthermore, the pH of the culture medium had a measurable effect on the ratio of synthesized mono- and dirhamnolipids. At pH values below 7.3, the proportion of monorhamnolipids decreased from 45 to 24%. The recycling of 20% of the spent culture medium in whereP. aeruginosawas grown up to the later stationary phase was responsible for a 100% increase in rhamnolipid volumetric productivity in the new culture medium. Finally, the use of fed-batch operation under conditions of limited nitrogen resulted in a 3.8-fold increase in the amount of rhamnolipids produced (2.9 g L−1–10.9 g L−1). These results offer promising pathways for the optimization of processes for the production of rhamnolipids.

scholarly journals Recent advancements in the production of rhamnolipid biosurfactants by Pseudomonas aeruginosa

RSC Advances ◽  
2020 ◽  
Vol 10 (56) ◽  
pp. 34014-34032
Author(s):  
Parisa Eslami ◽  
Hamidreza Hajfarajollah ◽  
Shayesteh Bazsefidpar
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Effective Surface ◽  
Surface Active Agents ◽  
Surface Active

Rhamnolipid (RL) biosurfactant which is produced by Pseudomonas species is one of the most effective surface-active agents investigated in the literature.

scholarly journals Strategies for improved rhamnolipid production by Pseudomonas aeruginosa PA1

2015 ◽  
Author(s):  
Alexandre Soares dos Santos ◽  
Nei Pereira Jr ◽  
Denise M G Freire
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Stationary Phase ◽  
Culture Medium ◽  
Fold Increase ◽  
Batch Process ◽  
Measurable Effect ◽  
Fed Batch ◽  
Batch Operation ◽  
Ph Values ◽  
Influence Of Ph

Rhamnolipids are biosurfactants with potential for diversified industrial and environmental uses. The present study evaluated three strategies to increase the production of rhamnolipid-type biosurfactants produced by Pseudomonas aeruginosa strain PA1. The influence of pH, the addition of endogenous autoinducers and the use of a fed batch process were examined. The culture medium adjusted to pH 7.0 was the most productive. Furthermore, the pH of the culture medium had a measurable effect on the ratio of mono- and dirhamnolipids synthesized. At pH values below 7.3, the proportion of monorhamnolipids decreased from 45 to 24%. Additionally, recycling 20% of the spent culture medium where P. aeruginosa was grown up to the later stationary phase was responsible for a 100% increase in rhamnolipid volumetric productivity in the new culture medium. Finally, the use of fed batch operation under conditions of limited nitrogen resulted in a 3.8-fold increase in the amount of rhamnolipids produced (1.29 g L-1 to 4.90 g L-1, as rhamnose). These results offer promising paths to optimize processes for the production of rhamnolipids.

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