Repeated Frying of Olive Oil Has a Better Effect on Survival Rate Compared to Palm Oil Through Glucose Energy Metabolism Increase in Mice

2020 ◽  
Vol 16 ◽  
Author(s):  
Nadiya Auliya Nabila ◽  
Sri Umijati ◽  
Lilik Herawati
Keyword(s):  
Blood Glucose ◽  
Survival Rate ◽  
Olive Oil ◽  
Glucose Level ◽  
Palm Oil ◽  
Unsaturated Fatty Acids ◽  
Fasting Blood Glucose ◽  
Control Group ◽  
Oral Glucose ◽  
Olive Oils

Background: Vegetable oil is widely used for frying food, especially palm oil. Repeated use of cooking oil is often unavoidable. Frying is known to change cis unsaturated fatty acids into trans. However,the effects of repeated frying of olive oil compared to palm oil is still not widely known. Objective: The objective of this study is to determine the effect of repeated frying of palm oil and olive oil on survival rate and blood glucose pattern in mice. Results: After 4 weeks of treatment, there were 9 mice that remain alive in the control group, 9 mice in the palm group, and 11 mice in the olive group. The survival rate was 75% in the control group, 69.23% in the palm group, and 78.57% in the olive group. Weight gain was significantly different between pre and post in the control and olive groups (p=0.009; p=0.004). FBG level increased significantly in the palm group compared to the control group (P<0.05). The 2-h OGTT glucose level increased significantly compared to pre in the control group (p=0.004) and olive group (p=0.000). AUC of the olive group increased significantly compared to the control group (p=0.0039). Methods: There were 39 adult male mice divided into 3 groups: control (n=12), palm (n=13), and olive (n=14). Control group was given oral gavage of water during research. Palm and olive oils were given in palm and olive groups. Before given to mice, palm and olive oils were used to fry sweet potatoes 5 times then given to mice for 4 weeks. At the beginning and after treatment, fasting blood glucose (FBG), the 2 hours postprandial of oral glucose tolerance test (2-h OGTT) blood glucose level were measured and then area under the curve (AUC) was also calculated. Conclusion: Consumption of olive oil which is fried repeatedly and given for 4 weeks shows a better survival rate than palm oil.

Anti-diabetic activity of diphenhydramine in diabetic rats

2020 ◽  
Vol 11 (4) ◽  
pp. 5067-5070
Author(s):  
Pang Jyh Chayng ◽  
Nurul Ain ◽  
Kaswandi Md Ambia ◽  
Rahim Md Noah
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Fasting Blood Glucose ◽  
Insulin Level ◽  
Diabetic Rats ◽  
Group Iv ◽  
Diabetic Rat ◽  
Control Group ◽  
Group I

The purpose of this project is to study the anti-diabetic effect of on a diabetic rat model. A total of Twenty male Sprague rats were used and it randomly distributed into four groups which are Group I: , Group II: negative control, Group III: and Group IV: and . In diabetic model were induced with via injection at the dosage of 65mg/kg. and FBG (Fasting Blood Glucose) level of diabetic rats were assessed every three days. Blood was collected via cardiac puncture at day 21 after the induction of treatment. Insulin level of the rats was assessed with the Mercodia Rat Insulin ELISA kit. FBG level of group I (12.16 ±3.96, p&lt;0.05) and group IV (11.34 ±3.67, p&lt;0.05) were significantly decreased. Meanwhile, the for all rats did not show any significant increase. However, the insulin level was escalated in group IV (0.74+0.25, p&lt;0.05) significantly. The present study shows that the and the combination of and lowered blood glucose level and enhanced insulin secretion.

scholarly journals Lactobacillus rhamnosus Reduces Blood Glucose Level through Downregulation of Gluconeogenesis Gene Expression in Streptozotocin-Induced Diabetic Rats

2020 ◽  
Vol 2020 ◽  
pp. 1-12
Author(s):  
Eko Farida ◽  
Lilis Nuraida ◽  
Puspo E. Giriwono ◽  
Betty S. L. Jenie
Keyword(s):  
Blood Glucose ◽  
Glucose Tolerance ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Fasting Blood Glucose ◽  
Lactobacillus Rhamnosus ◽  
Tolerance Test ◽  
Diabetic Rats ◽  
The Body ◽  
Oral Glucose

Some lactic acid bacteria (LAB) are observed to be potential probiotics with functional properties such as lowering fasting blood glucose (FBG), as a promising hyperglycemia management. This study investigated the ability and mechanism of Lactobacillus rhamnosus BSL and Lactobacillus rhamnosus R23 on lowering FBG in diabetic rats induced by streptozotocin (STZ). The rats were orally administered with L. rhamnosus BSL and L. rhamnosus R23 by giving 1 mL cell suspension (109 CFU/mL) daily for 30 days. The body weight (BW) was recorded once in three days, and FBG was recorded once in six days. An oral glucose tolerance test (OGTT) was measured 1 week after injection with STZ and before sacrifice. Fecal samples were collected on days 0, 15, and 30 for LAB population and identification, performed by PCR detecting 16S rRNA. Oral administration of L. rhamnosus BSL and L. rhamnosus R23 decreased FBG and improved glucose tolerance via downregulation of glucose-6-phosphatase (G6pc) expression by 0.57- and 0.60-fold change, respectively (P<0.05). The lipid profiles, BUN, creatinine, SGOT, and SGPT were significantly (P<0.05) different between normal and diabetic rats, but they were not significantly (P>0.05) different among diabetic rats. Both strains were effective in increasing fecal LAB population. Molecular identification of the isolated LAB from fecal sample indicated that they were able to survive and pass through the digestive tract. These results suggested that both strains have the ability to manage blood glucose level and become a promising agent to manage hyperglycemia and diabetes.

scholarly journals Hypoglycemic Effect of Nigella sativa L. (Ranunculaceae) Extract in Streptozocin (STZ) Induced Diabetic Rats

2020 ◽  
Vol 8 (02) ◽  
pp. 35-39
Author(s):  
Laxmi Shrestha ◽  
Amit Shrivastava ◽  
Bishal Joshi ◽  
Buddhi Raj Pokhrel ◽  
Shanti Gurung ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Aqueous Extract ◽  
Nigella Sativa ◽  
Negative Control ◽  
Hypoglycemic Effect ◽  
Control Group ◽  
Oral Glucose ◽  
Standard Group

INTRODUCTION Diabetes mellitus is the most common metabolic disease worldwide. Nigella sativa also known as black cumin, is an emerging miracle herb with a rich historical and religious background. In the present study, we have attempted to study the hypoglycemic effect of aqueous extract of Nigella sativa on diabetes-induced rats. MATERIAL AND METHODS This preclinical study was conducted in Department of Pharmacology from August to October 2019. In this study, hyperglycemia was induced by a single intraperitoneal dose of 60 mg/kg streptozotocin in 0.1 M citrate buffer (pH 4.5). Animals were divided into five groups containing normal control, negative control, standard, test-1, and test-2. The aqueous extract of Nigella sativa was administered 400 and 800 mg/kg per oral in both the test groups for 18 days. Blood glucose level was measured at three-days interval by glucometer. Oral glucose tolerance test was done by administrating 2 gm/kg per oral glucose and the blood glucose was recorded every two-hours interval for eight hours. RESULTS An oral glucose tolerance test showed a decrease in blood glucose level at the dose of 400 and 800 mg/kg compared to the negative control group. The administration of aqueous extract of Nigella sativa for 18 days showed significant decrease in blood glucose level (p=0.001) compared to negative control group. The blood glucose level in test 1 (400 mg/kg) was significantly reduced compared to the standard group (p***<0.001). The blood glucose level in test 2 at 18th day was less significant (p*< 0.05) compared with standard group and test 1 (p**<0.01, p***<0.001). CONCLUSION The findings of the present study indicate that the aqueous extract of Nigella sativa has significant hypoglycemic effect in Wistar rats.

scholarly journals The Effect of Belimbing Wuluh (Averrhoa Bilimbi L.) and Red Guava (Psidium Guajava L.) Extract on Fasting Blood Glucose Levels of Diabetic Wistar Rats Model

2019 ◽  
Vol 5 (2) ◽  
pp. 57
Author(s):  
Yesi Warisman ◽  
Arinda Lironika Suryana ◽  
Zora Olivia
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Wistar Rats ◽  
Fasting Blood Glucose ◽  
Diabetic Rats ◽  
Control Group ◽  
Standard Diet ◽  
Glucose Levels ◽  
Blood Glucose Levels

Diabetes mellitus is a metabolic disease with special characteristic which increased blood glucose level (hiperglicemic), its happens because of the abnormality of insulin secretion, insulin mechanisme or both of them. Belimbing wuluh and red guava extract can become alternative functional drink that contain rich of vitamin C to help controlling blood glucose level. The aimed of this study was to determined the effect of belimbing wuluh and red guava extract on blood glucose levels of Rattus novergicus strain Wistar diabetic model. This research was an true experimental study with a randomized post-test control group design. Twentyfour (24) male wistar strain rats weighing 200-250 grams, aged 2-3 months, were divided into 4 groups. The control group (K-) non-diabetic rats, the control group (K+) diabetic rats given standard diet, and the treatment group diabetic rats given standard diet and belimbing wuluh and red guava extract intervetion doses 10,7 ml/day (P1) and 21ml/day (P2). Measurement of pretest posttest fasting blood glucose levels was carried out using biolyzer. The data were analyzed by One Way Anova test. The results showed that there were significant differences in delta pretest and posttest fasting blood glucose levels between all groups. Blood glucose levels decreased at 55.6%. In conclusion, belimbing wuluh and red guava extract 21ml/day most effectively decreased fasting blood glucose levels in diabetic wistar rats moldel.   Keywords: belimbing wuluh, blood glucose level, diabetes mellitus, red guava

scholarly journals Antidiabetic and Antioxidant Activities of the Twigs of Andrograhis paniculata on Streptozotocin-Induced Diabetic Male Rats

BioChem ◽  
2021 ◽  
Vol 1 (3) ◽  
pp. 238-249
Author(s):  
Olubanke O. Ogunlana ◽  
Babatunde O. Adetuyi ◽  
Elohor F. Esalomi ◽  
Miracle I. Rotimi ◽  
Jacob O. Popoola ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Antioxidant Activities ◽  
Fasting Blood Glucose ◽  
Male Rats ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Albino Rats ◽  
Oral Glucose ◽  
Distilled Water ◽  
Group 2

Background: Diabetes is associated with chronic hyperglycaemia, long-term damage, dysfunction, and organ failure. This study aims to evaluate the antidiabetic activity of the twigs of Andrographis paniculata and its toxicological markers on Streptozotocin (STZ)-induced diabetic Albino rats. Methods: A total of thirty rats were randomly divided into five groups of six animals each. Non-diabetic animals were treated with distilled water as non-diabetic sham control group 1, while diabetic animals (group 2, 3, 4 and 5) were treated with 60 mg/kg bw STZ intravenous (iv) and 100 mg/kg body weight (bwt) of metformin orally for group 2, distilled water for group 3, and 250 and 500 mg/kg bwt of Andrograhis paniculata (A. paniculata) for groups 4 and 5, respectively. The animals were dosed for 28 days, after which they were sacrificed. Liver and kidney function tests as well as livid profile tests were used as the biomarkers of toxicological assessment. Fasting blood glucose was carried out weekly. Oral Glucose Tolerance Test (OGTT) was conducted on the 28th day of the antidiabetic assessment. Results: A. paniculata groups 4 and 5 were significant at different doses (p < 0.05) in reducing the blood glucose level in comparison with metformin. There were significant changes in total and direct bilirubin, total protein, potassium, triglyceride and inorganic phosphorus in 500 mg/kg bwt of the treated group in comparison with the metformin and diabetic group groups. A. paniculata at 500 mg/kg bwt is most effective for its antidiabetic and organ protecting effects.

scholarly journals Effects of ginger juice on blood glucose in alloxan induced diabetes mellitus in rats

2015 ◽  
Vol 23 (1) ◽  
pp. 14-17 ◽  
Author(s):  
Selima Sultana ◽  
Md Ismail Khan ◽  
Hasanur Rahman ◽  
Abu Sadat Mohammad Nurunnabi ◽  
Rokhsana Dil Afroz
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Fasting Blood Glucose ◽  
Zingiber Officinale ◽  
Control Group ◽  
Fasting Blood Glucose Level ◽  
Medical College ◽  
Group D

Objective: To find out the effects of ginger (Zingiber officinale) juice blood glucose in alloxan induced-diabetes mellitus in rats. Methods: This experimental study was done in the Department of Pharmacology & Therapeutics, Dhaka Medical College, Dhaka, in collaboration with the Departments of Pathology, Ibrahim Medical College, Dhaka between January and December 2009. This experimental animal study was divided into two parts, which were Experiment-1 and Experiment-2. Experiment 1 comprises of 12 rats and divided into 2 groups each group having 6 rats. Rats of group-A was non-diabetic normal control group and group-B was fed with ginger (Zingiber officinale) in a dose of 4ml/kg body weight orally through Ryle’s tube. Experiment-2 comprised of 12 rats divided into 2 groups each containing 6 rats labeled as group C, group D. Rats of groups C administered alloxan 150 mg/kg intraperitoneally on the 2nd day of the study. Rats of group D were administered alloxan 150mg /kg intraperitoneally and ginger (4ml/kg of body weight orally) on the 2nd day of the study. Results: The fasting blood glucose level at day 12 in the rats treated with ginger (Zingiber officinale) 4 ml /kg body weight orally daily for 12 days showed reduction in fasting blood glucose level as compared to control group, but not significant, which indicates that ginger has no effect in lowering blood glucose of normal rats. The fasting blood glucose levels at day 12 in the rats of group D (treated with ginger and alloxan) showed highly significant reduction in fasting blood glucose level as compared to diabetic control group (p<0.002). Conclusion: Consumption of ginger produced a significant antihyperglycemic effect in experimentally induced diabetic rats. DOI: http://dx.doi.org/10.3329/jdmc.v23i1.22687 J Dhaka Medical College, Vol. 23, No.1, April, 2014, Page 14-17

AKTIVITAS KOMBINASI EKSTRAK BAWANG DAYAK-METFORMIN TERHADAP GULA DARAH MENCIT

2018 ◽  
Vol 4 (2) ◽  
pp. 114
Author(s):  
Aditya Maulana Perdana Putra ◽  
Ratih Sari Pratiwi
Keyword(s):  
Diabetes Mellitus ◽  
Blood Glucose ◽  
Glucose Level ◽  
Fasting Blood Glucose ◽  
Test Group ◽  
Control Group ◽  
Onion Extract ◽  
Glucose Levels ◽  
Reduce Blood Glucose ◽  
Average Glucose

Diabetes mellitus is a chronic disease that requires good medical treatment to prevent long-term complications. The proportion of DM patients in Indonesia in 2013 was 3,7 million. Indonesia as one of the countries with the highest diversity has natural resources that can be used as an alternative medicine for diabetes mellitus, one of them is dayak onion. Dayak onions themselves contain compounds that can reduce blood glucose levels. The use of traditional medicine in the community is sometimes combined with chemical drugs. So the purpose of this study was to determine the comparison of the combination activity of dayak onion  extract – metformin with single metformin to reduce mice blood sugar induced by alloxan. This research includes experimental research. The test animals used were male white mice which were divided into 2 groups, namely the combination of dayak onion and metformin and the single metformin group. The dose of dayak onion extract used in this study was 100 mg / KgBB and the dose of Metformin used was 45 mg / KgBB. Measurement of glucose levels is carried out every 3 days for 15 days. The decrease in fasting blood glucose from the control group and the test group was analyzed by the General Linear Model test at a 95% confidence level. The results of this study indicate that the combination of dayak onion tuber extract and metformin has better hypoglycemic activity than single metformin. This result can be seen from the average glucose level from day 3 to 15 the combination of dayak onion tuber and metformin is lower than the average glucose level of single metformin. The results of GLM analysis showed the sigvalue <0,05 so it can be concluded that there is a difference in glucose levels between the combination of dayak onion tuber and metformin with a single metformin.

Enhanced myocardial depression in diabetic rats during E. coli sepsis

1987 ◽  
Vol 253 (2) ◽  
pp. H276-H282
Author(s):  
K. H. McDonough ◽  
R. W. Barbee ◽  
C. Dobrescu ◽  
C. H. Lang ◽  
J. J. Spitzer
Keyword(s):  
Blood Glucose ◽  
Fasting Blood Glucose ◽  
Systolic Pressure ◽  
Cardiac Performance ◽  
Diabetic Group ◽  
Control Group ◽  
Oral Glucose ◽  
Glucose Load ◽  
E Coli ◽  
Abnormal Response

The aim of this study was to determine whether diabetes enhanced the sensitivity of the myocardium to the deleterious effects of in vivo-administered Escherichia coli. Diabetes was induced in two groups of animals. One group received 70 mg/kg streptozotocin (iv) and exhibited a severe diabetes with elevated fasting and fed blood glucose concentrations and a markedly abnormal response to an oral glucose load. The second group received 45 mg/kg streptozotocin, was mildly diabetic (termed “latent” diabetes), and was characterized by normal fasting blood glucose but slightly elevated fed blood glucose and an abnormal response to a glucose load. A third group of rats received vehicle and served as time-matched control animals. Four weeks after induction of diabetes, all animals were catheterized under ether anesthesia and some received intraperitoneal injections of live E. coli. In vitro myocardial performance was assessed using the isolated, perfused working heart preparation. Ventricular function curves were generated by changing left atrial filling pressure and measuring changes in heart rate, cardiac output, and aortic peak systolic pressure. Cardiac performance in the severe diabetic group was depressed at the highest preload but was unchanged at lower preloads. Function in the latent diabetic group was not different from control. Sepsis induced a slight decrease in cardiac performance in the control group and resulted in larger reductions in the latent and severe diabetic groups. A depression in aortic flow was the major consequence of sepsis in the latent diabetic group, whereas decreased coronary flow was the primary change in the severe diabetic group.(ABSTRACT TRUNCATED AT 250 WORDS)

Psychiatric Aspects of Diabetes—a Physician's View

1981 ◽  
Vol 139 (6) ◽  
pp. 485-493 ◽  
Author(s):  
R. B. Tattersall
Keyword(s):  
Blood Glucose ◽  
Glucose Tolerance ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Common Factor ◽  
Fasting Blood Glucose ◽  
Normal Glucose Tolerance ◽  
Oral Glucose ◽  
Glucose Load ◽  
Normal Glucose

A raised blood sugar level no more defines a single entity than does a raised bilirubin or a low haemoglobin. Diabetes is a heterogenous group of disorders whose only common factor is hyperglycaemia (Tattersallet al, 1980). The classification of diabetes is being revised, although the changes are of more relevance to epidemiologists than clinicians. Previous standards of normal glucose tolerance were set too low, so that some people were labelled diabetic who had no symptoms and have proved on follow-up not to be at risk of developing complications such as retinopathy (i.e. they had a non-disease). Epidemiological evidence suggests that the cut-off point for ‘true’ diabetes (i.e. a condition which leads to complications and shortening of life span) is a blood glucose level two hours after a 50 G oral glucose load of 11.1 mMol/L (National Diabetes Data Group, 1979). This corresponds to a fasting blood glucose level of 7 mMol/L or below. Hence, a single blood glucose value, either in the fasting state or two hours after a 50 G glucose load, is enough to diagnose diabetes and glucose tolerance tests should hardly ever be necessary.

scholarly journals PENGARUH PEMBERIAN TRANS FATTY ACID (TFA) DARI MARGARIN DAN MINYAK KELAPA SAWIT YANG DIPANASKAN BERULANG TERHADAP KADAR GLUKOSA DARAH PUASA PADA TIKUS WISTAR

2017 ◽  
Vol 11 (1) ◽  
pp. 69
Author(s):  
Retno Larasati ◽  
Bambang Wirjatmadi ◽  
Merryna Adriani
Keyword(s):  
Fatty Acid ◽  
Blood Glucose ◽  
Trans Fatty Acid ◽  
Palm Oil ◽  
Fasting Blood Glucose ◽  
Control Group ◽  
Control Group Design ◽  
Treatment Groups ◽  
Glucose Levels ◽  
Blood Glucose Levels

The purpose of this study was to know differences in fasting blood glucose levels among both the control and treatment groups were given Trans Fatty Acid of groceries margarine and oil is heated rapidly. This research is true experimental design to study Post Test Only Control Group Design. The sample size in this study was 25 rats wistar strain males were divided into 5 groups: the first group was the control, the second group by of margarine that is heated by the content of TFA 1%, the third group by the addition palm oil, which is heated repeats to content TFA 1%, the fourth group by margarine are heated with TFA content of 2%, and the fifth group by the addition of palm oil, which is heated repeats to the TFA content of 2%. Treatment was given for 4 weeks, after the laboratory examination of fasting blood glucose at the end of the treatment. Data fasting blood glucose levels in all groups were tested by one-way ANOVA with 95% confidence level. The results showed that TFA may increase the levels of fasting blood glucose treatment groups compared with the control group, and the differences in levels of fasting blood glucose between treatment groups was not influenced by the amount of content TFA but due to different types of TFA given that of margarine and palm oil that is heated Repeat.Keywords: fasting glucose, TFA, margarine, oil heated, rats

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