Serum Endocrine Profile in Relation to Estrus Synchronization in Surti Does

Author(s):  
M. M. Chaudhary ◽  
C. T. Khasatiya ◽  
S. B. Patel ◽  
S. S. Chaudhary ◽  
V. B. Atara ◽  
...  

The serum progesterone and estradiol profiles during synchronization of estrus by buck effect and PGF2α treatments were monitored in Surti does. Total eighteen non-pregnant does selected were evenly divided into 3 groups, 6 does in each group. The does of Group I were teased with a sexuallyactive- apronized buck; and those of Group II were treated with PGF2α, i.e., Inj. Lutalyse® @ 7.5 mg/doe IM twice 11 days apart, while the Group III served as untreated control. Blood samples were collected from all the animals on day 0 (before 1st PGF2α injection), 3rd day (during treatment), 11th day (before 2nd PGF2α injection), 14th day (after treatment) and 40th day (post-service) by jugular vein puncture. The serum separated was stored at -20°C till further analysis. In all the three groups, 83.33% does, conceived at first service in the sampling cycle. The overall mean serum progesterone concentration of Group I does (5.82±0.72 ng/ml) was significantly higher (p less than 0.01) as compared to Group II (2.93±0.38 ng/ml) and III (2.88±0.30 ng/ml). Similarly, the overall mean serum progesterone concentration of Surti does on day 0 (2.65±0.46 ng/ml), 3rd (2.56±0.80 ng/ml), 11th (4.45±0.84 ng/ml) and 14th (3.40±0.63 ng/ml) did not differ significantly, but the overall mean level at day 40 (6.31±0.45 ng/ml) was significantly (p less than 0.01) higher, because most of animals became pregnant at that time. The overall mean serum oestradiol-17β levels of Group I (24.40±2.98 pg/ ml) was significantly higher (p less than 0.01) than in Group II (15.77±1.77 pg/ml) and III (12.21±1.45 pg/ ml). On the other hand, the overall mean serum oestradiol-17β levels of Surti does on day 0 (12.89±1.21 pg/ml), 3rd (15.84±1.74 pg/ml), 11th (14.81±1.96 pg/ml), 14th (22.15±2.97 pg/ml) and 40th (21.64±5.16 pg/ml) did not differ significantly (p>0.05) and the slightly higher overall mean level found at 40th day might be the influence of the non-pregnant does at first service in the cumulative animals. The hormonal profile reflected the initiation of cyclicity and establishment of pregnancy in treated and control animals.

2021 ◽  
Vol 9 (3) ◽  
pp. 87-90
Author(s):  
A. Jyoth ◽  

The effect of fiber rich product on child hood obesity studied with 60 sample which were collected randomly among 10-12 years and categorized into 2 groups i.e, experimental group and control group. Experimental group further categorized into three groups i.e, supplementation with exercise (n=15), only supplementation group (n=15), only exercise group (n=15) and control group consists of (n=15).Anthropometric, and diet survey conducted as parameters. A fiber rich product was prepared and supplemented for 2 months to the selected subjects and it consists of whole Bengal gram, whole green gram, green peas, barley and jaggery. Positive results were obtained in three experimental groups. Significant decrease observed inweight, and BMI, Total cholesterol, Triglycerides, LDL, VLDL significant increase observed in HDL in group I II and III. The results were (51.60-48.26kg, 24.7-23.1, 195.2-152.3 mg/dl, 168.2-145.0 mg/dl, 52.2-54.13 mg/dl, 109.4- 69.4mg/dl, 33.6-28.7mg/dl) in group I, In group II the results are (50.3-49.86kg, 23.7-23.4, 168.6-161.0mg/dl, 145.4-129.6mg/dl, 44.2-45.2 mg/dl, 95.3-90.0mg/dl, 29.1-28.3mg/dl).In group III the tesults aere (50.7-49.6kg, 24.5-23.9, 143.2-139.3mg/dl, 139-134.5mg/dl, 38.2-38.7mg/dl 76.8-74.1 mg/dl, 25.6-28.1mg/dl) .From the results it was clear that when compared to group II and III group I has shown better results.


2012 ◽  
Vol 24 (1) ◽  
pp. 140
Author(s):  
H. Stinshoff ◽  
E. Onnen-Lübben ◽  
S. Wilkening ◽  
A. Hanstedt ◽  
H. Bollwein ◽  
...  

Shortly after parturition the metabolic situation of high-yielding dairy cows is often dominated by a negative energy balance. These effects affect the whole animal and may especially be detected in the reproductive tract, where they result in reduced fertility. An oral supplementation with dietary fats is often used to counteract by reducing milk fat content and, thus, supplying the individual animal with an increased amount of energy. The focus of the present study was to analyse the effects of an oral supplementation with conjugated linoleic acids (CLA) on corpus luteum (CL) function. Healthy Holstein-Friesian cows and heifers were randomly allocated to 2 treatment groups (Group 1: 50 g of CLA day–1 per animal, 2 heifers, 6 cows; Group 2: 100 g of CLA day–1 per animal, 2 heifers, 6 cows) and 1 control group (Ctl; 0 g of CLA day–1 per animal, 3 heifers, 4 cows). Feeding of the supplement began shortly after calving. After calving, all animals were subjected to a standard synchronisation protocol and experienced AI on Day 59 ± 3. Following AI, transvaginal biopsies of the corpus luteum were obtained of pregnant (Group I: n = 4; Group II: n = 4; Ctl: n = 4) and nonpregnant (Group I: n = 4; Group II: n = 4; Ctl: n = 3) animals on Days 6, 13 and 20 post-AI. Animals deemed pregnant on Day 28 were again biopsied on Day 42. Additionally, blood samples were taken from the vena sacralis mediana at the time of each biopsy. The biopsies were analysed regarding the relative abundance of 8 gene transcripts (VEGF, ECE1, PLA2G4A, PTGS2, PTGFR, PPARG, STAR and HSD3B1) via RT-qPCR. Blood samples were analysed for their concentration of progesterone through a radioimmunoassay (RIA). Statistical analysis for both datasets was performed via a 3-way ANOVA with adjoining Tukey test. The expression of 7 of these genes was affected by 1, 2, or all 3 of the following factors: day of cycle (VEGF, ECE1, PLA2G4A, PTGFR, STAR and HSD3B1), pregnancy status (ECE1, PTGFR and HSD3B1) and CLA supplementation (ECE1, PTGS2, PTGFR, STAR and HSD3B1). The effects of the CLA supplementation could be seen as a down-regulation in the mentioned gene transcripts. Progesterone concentrations differed significantly in dependency of the pregnancy status (significantly higher in pregnant vs nonpregnant individuals) of the animals, as well as during the days of the oestrous cycle (physiological progesterone curve with highest values on Day 13 of these samples). An effect of the oral supplementation with CLA could be detected during the early luteal phase (Day 6) where animals that had received 100 g of CLA day–1 had a significantly lower blood progesterone concentration than those receiving 50 g of CLA day–1 or no CLA. In conclusion, dietary CLA supplementation has an effect on luteal gene expression and functionality. The authors thank the DFG (German Research Foundation) for their financial support (PAK286/1; WR154/1-1).


2007 ◽  
Vol 12 (2) ◽  
pp. 187-189
Author(s):  
Indah Norma Triana
Keyword(s):  
Group Iv ◽  
Male Rat ◽  
Group I ◽  
Group Ii ◽  

The experiment was conducted the potential of antiibody sperm to spermatogenesis and fertility of the male rat. Ten of rat testis have been used in this research, then crushed and added with 10 cc saline and then were filtrated and centrifuged 1800 rpm for ten minutes. Twenty four of the male rat divided in to four groups. Group I injected with saline 0.1 cc/sc/day for 45 days, group II injected with testis suspension 0.1 cc/sc/day for 15 days, group III injected with testis suspension 0.1 cc/sc/days for 30 days, group IV injected with testis suspension 0.1 cc /sc/days for 45 days. Then each of the male rat were mated with female, then the number of the total of pups were counted. Male rat were killed and testis were collected for preparation of histology and count of the spermatocyte and spermatid. The results show that the number of the spermatocyte and spermatid has significant different in the group and control (p less than 0.05). The number of the pups significantly different in the group and control (p less than 0.05). Implication of this research was injected Antibodisperm on male rat can decrease of the number of spermatocyte, spermatid and the number of the pups.


Author(s):  
Sumit Singhal ◽  
Shiv Prasad ◽  
Rajesh Verma ◽  
H.P. Gupta ◽  
J.K. Prasad

Background: Limited elite buffalo population need to be propagated at faster rate to sustain the dairy industry in India. Faster propagation could be achieved through embryo transfer technology. Low serum progesterone concentrations during the start of superstimulatory treatment is major factor that hampers the success of multiple ovulation embryo transfer (MOET) in buffaloes. This study evaluated the effect of GnRH pretreatment before superovulatory regimen on progesterone profile, superovulation and correlation of progesterone with superstimulatory parameter in Murrah buffaloes. Methods: Buffaloes (n=27) were superstimulated using 600 mg Folltropin and divided into three groups on basis of pretreatment: Group I and group II were pretreated with GnRH @ 10 µg and 06 µg, respectively while no pretreatment was subjected in group III (control). Progesterone hormone concentration, superovulatory response, ovulation rate, embryo recovery were recorded. Result: Our study revealed that superovulatory response was non-significantly different in all three groups and ranged from 6-8 in terms of number of corpus luteum (CL). Average number of recovered embryos in this study was nearly 2.0. A positive correlation between progesterone concentrations at the initiation of FSH treatment with number of CL and embryo recovery was observed. The study indicated that GnRH pretreatment before superstimulation improve the serum progesterone concentration, superovulatory response and embryo recovery in Murrah buffaloes. 


Author(s):  
R. J. Raval ◽  
K. B. Vala ◽  
R. J. Padodara ◽  
A. J. Dhami ◽  
F. S. Kavani

Background: Anestrus is one of the most commonly encountered infertility problems in cattle and buffalo in India. Jaffarabadi is one of the heaviest buffalo breeds of the world and is a native of Saurashtra region of Gujarat. The breed is known for poor reproductive efficiency. To improve its reproductive efficiency, this study was undertaken on acyclic Jaffarabadi animals employing double Ovsynch protocol and its assessment through ovarian dynamics and blood biochemical and endocrine profile on a farm. Methods: The study included acyclic post-pubertal Jaffarabadi heifers (age 42±2 and 48±2 months; Gr-I and Gr-II, n=6 each) and the postpartum lactating acyclic buffaloes (Gr-III, n=6) using a double Ovsynch protocol, which consisted of i/m injections of 20 µg Buserelin acetate on days 0, 10, 17 and 26 and 500 µg Cloprostenol sodium on days 7 and 24, with a timed insemination on day 27. Ovarian dynamics was monitored by performing ultrasonography using real-time B-mode ultrasound scanner together with blood sampling for hormonal and biochemical profile on each day of hormone therapy and then on days 0, 12, 21 and 35 post-AI. The animals inseminated at induced estrus/FTAI were followed for return to estrus, if any. Pregnancy was confirmed in non-return cases on day 35 by ultrasonography and on day 70 by per rectal examination. The findings on ovarian dynamics, plasma endocrine and biochemical profile were compared statistically between groups and periods and fertility rates between groups. Result: In animals of treatment group I and II, a significant (p less than 0.05) increase in numbers of small follicles was observed on day 26. Number of large sized follicles was significantly (p less than 0.05) higher on day 26 in comparison to day 0 and day 7 in group III animals. Large and subordinate follicular diameters increased gradually, but the differences between periods were significant (p less than 0.001) only in group III with the highest diameter of large follicle on day 17. Plasma FSH and LH concentrations differed significantly (p less than 0.001) among groups at all-time intervals. Plasma LH in group I and III differed significantly (p less than 0.001). Plasma estrogen level was significantly (p less than 0.05) higher in group II than group III. Plasma progesterone concentrations in group I and II animals were significantly higher on day 35 post-AI. Plasma insulin levels were significantly (p less than 0.01) lower on all days for group III animals than group I and II. Blood glucose level was significantly (p less than 0.001) higher in group I on day 17 as compared to group II and III. Plasma total cholesterol was significantly (p less than 0.05) higher in group III as compared to group I and II. The conception rates at first service/FTAI in double Ovsynch treated animals of group I, II and III were 66.66, 83.33 and 16.66 %, respectively. Thus it was concluded that ovarian structures and the plasma endocrine profile reflected the ovarian response to different hormonal injections and that double Ovsynch protocol could be a better choice for improving conception rate in post-pubertal acyclic Jaffarabadi buffalo heifers as compared to multiparous acyclic buffaloes.


1986 ◽  
Vol 109 (1) ◽  
pp. 107-110
Author(s):  
R. A. Nowak ◽  
J. S. Klein ◽  
D. M. Pulido ◽  
J. M. Bahr

ABSTRACT The present study was undertaken to determine (1) whether the rabbit feto-placental unit maintains corpora lutea systematically and/or locally and (2) the interrelationships between conceptus number, luteal weight, luteal progesterone concentrations and serum progesterone levels. Thirty-three does were divided into the following treatment groups: (I) bilaterally pregnant, two ovaries; (II) unilaterally pregnant, two ovaries; (III) bilaterally pregnant, one ovary; (IV) unilaterally pregnant, one ovary, contralateral and (V) unilaterally pregnant, one ovary, ipsilateral. Blood samples were obtained from all rabbits on days 6, 9, 12, 15, 18 and 21 post coitum. Does were killed on day 21, and the percentage of viable fetuses, fetal weights, and luteal weights recorded. Blood samples and corpora lutea were analysed for progesterone. Serum progesterone levels were similar for all groups until day 9 post coitum. Levels in groups III, IV and V declined significantly between days 9 and 12 following removal of one ovary at day 9. Fetal viability, fetal weights and luteal progesterone concentrations did not differ among any of the groups. Luteal weights did not differ among groups I, III, IV and V, but luteal weights of animals in group II were lower than those of group I (P<0·05). Ratios of viable fetuses to number of corpora lutea ranged from 1:11–10:5. No differences were observed in serum progesterone, luteal weights or luteal progesterone concentrations among animals with two conceptuses and those with seven or more, but serum progesterone levels in does with only one conceptus were lower than those in does with more (P<0·05). These results indicate that the feto-placental unit maintains corpora lutea systemically and that the high rate of pregnancy failure by day 21 in does with only one conceptus is due to the inability of a single conceptus to maintain normal serum progesterone levels even though the corpus luteum weight is not affected. J. Endocr. (1986) 109, 107–110


2020 ◽  
pp. 1-3
Author(s):  
Semmalar. R ◽  
Jain Vanitha. N. S ◽  
Fabiola M. Dhanraj ◽  
Anitha Rajendra Babu ◽  
Susila Susila

An experimental post test only control design was used to assess the effectiveness of selected interventions on pain perception and parental satisfaction among infants receiving immunizations at Public Health Centre, Chennai. Using simple random sampling method, the total of 40 infants were allocated in four groups where each group had 10 infants. The interventions provided are oral sucrose for Experimental group I, 4 S’s technique for experimental group II, oral sucrose with 4 S’s technique for Experimental group III and routine care for Control group. The data collection tool consists of demographic profile, modified riley pain scale and parental satisfaction likert scale were used to collect data. The result of the study revealed that mean pain perception of experimental group III (3.21) was less than the experimental group I (4.08), experimental group II (3.40) and control group (4.22). There was no statistical difference between experimental group II and experimental group III at the level of 0.693 and also between experimental group I and control group at the level of 1.000 when other groups had statistically significant difference in pain perception. In mean parental satisfaction, the experimental group III (43.20) had high parental satisfaction than the experimental group I (39.80), experimental group II (41.90) and control group (38.60).Hence the pilot study concluded that 4S’s technique was the effective intervention for infant immunizations.


Author(s):  
S. I. Smiian ◽  
B. O. Koshak ◽  
U. S. Slaba

Mortality rate among patients with AS is 2 times higher the population level. It is caused by cardiovascular disease. Now it is clear that the presence of chronic systemic inflammation is an important predictor of cardiovascular (CV) disease due to the development of endothelial dysfunction (ED).The aim of the study – to evaluate the efficacy of correction of ED in patients with AS and arterial hypertension with the use of quinapril and L-arginine aspartate.Materials and Methods. A total of 110 patients with AS were examined with evaluation endothelium-depended vasodilation (EDVD) in response to reactive hyperemia, were chosen patients with EDVD <10 % (n=79), and were divided into 4 groups. Group I included 17 people who received standard therapy. Patients from the group II (n=20) received intravenous infusion of the solution of L-Arginine. Group III included patients with concomitant hypertension and they received quinapril (n=19). Group IV (n=23) consist of patients with hypertension, but their treatment was combination of L-arginine with quinapril.Results and Discussion. It was found that in the group II there was a significant improvement in the endothelium’s function (EDVD (23.2±2.4) % (p=0.0023), and its normalization were in 7 patients. In patients of the third group, who received only quinapril were not found normalization of EDVD in any of the patients. In last group (IV) 7 patients showed normalization of the endothelium, 10 improvements compared with baseline levels, and 6 no changes were observed.Conclusions. L-arginine in the complex therapy of patients with AC can significantly reduce the manifestations of ED in patients without concomitant hypertension. Combined use of quinapril in combination with L-arginine demonstrated improvement of endothelial function and control of blood pressure in patients with hypertension.


Author(s):  
Rupesh Raval ◽  
Kiran Parmar ◽  
Karshan Vala ◽  
Gajendra Solanki ◽  
S. S. Parikh

The relative efficacy of different treatment modalities was evaluated for induction of estrus in 32 post-pubertal true anestrus Jafarabadi buffalo heifers randomly divided into four equal groups. Animals of Group I received intravaginal CIDR and i/m injection of 1.0 mg estradiol valerate on day 0, i/m injection of 500 μg PGF2α on day 7 while removing CIDR, and 0.75 mg estradiol valerate on day 8. Fix timed insemination (FTAI) was performed at 48 and 72 h following PGF2α injection. Animals of Group II received standard Ovsynch protocol with FTAI. In Group III, Prajana HS 3 caps/ day for 3 days along with i/m injection of Vitamin AD3E (5 ml) and Tonophosphan (15 ml) on first day were administered. Group IV animals received no treatment and served as control. Among all inseminated heifers, in non-return cases pregnancy was confirmed per rectum 60 days postbreeding. The estrus induction response in Group I and II animals was 100 %, whereas in Group III and IV it was 37.5 % and 12.5%, respectively. In Group I, one animal conceived at induced estrus and another four at second service while in Group II, five animals conceived at second service giving overall conception rate (CR) of 62.5% in each group within 77 days of treatment. In Group III, three animals conceived by 70 days and in Group IV, only one animal (12.5%) showed estrus and conceived by 50 days of treatment with overall CR of 37.5% and 12.5%, respectively. Mean plasma progesterone concentration was significantly (p less than 0.05) higher on day 7 as compared to day 0, at estrus and on day 20 post-AI in all the animals. It was concluded that both CIDR and Ovsynch protocols are better than herbal heat inducer + supplements in inducing fertile estrus in anestrus buffalo heifers.


2020 ◽  
Vol 20 (2) ◽  
Author(s):  
Novika Ayuni Rambe ◽  
Tongku Nizwan Siregar ◽  
Teuku Armansyah TR ◽  
Gholib Gholib ◽  
Budianto Panjaitan ◽  
...  

ABSTRAK. Penelitian ini bertujuan mengetahui pengaruh perbedaan PGF2α analog terhadap persentase berahi sapi di Labuhanbatu Selatan. Sampel yang digunakan dalam penelitian ini adalah data hasil UPSUS SIWAB tahun 2017. Dalam penelitian ini digunakan data 2.547 ekor sapi yang memiliki variasi breed dan umur, dengan skor kondisi tubuh baik. Sapi-sapi dikelompokkan menjadi tiga kelompok berdasarkan preparat sinkronisasi berahi yang digunakan. K1= kelompok sapi yang diinjeksi dengan 5 ml PGF2α-1 (dinoprost tromethamine 5 mg/ml dan benzil alkohol 1,65%) berjumlah 1.300 ekor. K2= kelompok sapi yang diinjeksi dengan 5 ml PGF2α-2 (dinoprost tromethamine 5,5 mg/ml dan benzil alkohol 12,0 mg/ml) berjumlah 600 ekor. K3= kelompok sapi yang diinjeksi dengan 2 ml PGF2α-3 (cloprostenol 75 mg/ml dan chlorocresol 1,0 mg/ml) berjumlah 647 ekor. Penyuntikan dilakukan secara intramuskulus, dua kali dengan interval 10 hari. Data yang diperoleh kemudian dianalisis menggunakan uji Kruskal Wallis dan dilanjutkan dengan uji Mann Withney. Hasil analisis menunjukkan persentase berahi pada kelompok 1; 2; dan 3 masing-masing 80,7%; 50% dan 61,8% (P0,01). Dari hasil penelitian dapat disimpulkan bahwa PGF2α-1 mempunyai efektivitas lebih baik dibandingkan PGF2α-2 dan PGF2α-3 dalam induksi sinkronisasi berahi pada sapi di Kabupaten Labuhanbatu Selatan, Sumatera Utara.  (Effectiveness of different commercial prostaglandin hormone administration on the percentage of estrous in cattles in South Labuhanbatu, North Sumatera) ABSTRACT. The aim of this study was to determine the effect of differences PGF2α analogues on the percentage of estrus in cattles in South Labuhanbatu. The sample used in this study was the 2017 SIWAB UPSUS data. Data of 2,547 cattles with various breed and age as well as in good body condition scores were selected. The cattles were grouped into three data groups based on the hormone used for estrus synchronization. The cattles in data group I (1300 cows) were injected with 5 ml PGF2α-1 (dinoprost tromethamine 5 mg/ml and benzyl alcohol 1.65%). The cattles in data group II (600 cows) were injected with 5 ml PGF2α-2 (5.5 mg/ml dinoprost tromethamine and 12.0 mg/ml benzyl alcohol). The cattles in data group III (647) were injected with 2 ml of PGF2α-3 (cloprostenol 75 µg/ml and chlorocresol 1.0 mg/ml). Injections were carried out intramuscularly, twice at 10-day intervals. The data obtained were then analyzed Kruskal Wallis and continued with Mann Withney test. The results showed that the percentage of estrus in groups I; II; and III were 80.7%; 50% and 61.8% (P0.01). From the results of the study it can be concluded that Lutalyse has better effectiveness than Capriglandin and Sincrovall in the induction of synchronization estrus in cattle in South Labuhanbatu, North Sumatera.


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