scholarly journals PRESENTATION PATTERN AND FUNGAL AGENTS SPECTRUM CAUSING OTOMMYCOSIS

Author(s):  
Kassim Dekhil

 Objective: This study was aimed to identify the public pattern of presentation, influencing factors, and sort the fungal species, distribution of sex of patients with otomycosis.Results: The predominant complaints were pruritus and found in 76 patients (88.73%), discomfort and pain found in 62 patients (72.09%), aural fullness in 48 patients (55.81%), tinnitus in 34 patients (39.53%), hearing impairment in 50 cases (58.31%), ear discharge in 22 patients (25.58%), and most of the symptoms seen in 36 patients (68.14%). The results showed a total of eight fungal species belong to six different genera, namely, Aspergillus, Candida, Penicillium, Rhizopus, Alternaria, and Cephalosporium were isolated during this study. Among identified fungi, Aspergillus niger was found to be the most prevalent fungal species with 35.71% followed by Candida albicans (27.55%), Aspergillus flavus (10.20%), Aspergillus fumigatus (8.16), Penicillium digitatum (6.12%) and Cephalosporium species (4.08%), and Rhizopus species (5.1%), while Alternaria alternata had the lowest percentage (6.54%).Conclusion: Otomycosis/mycotic otitis externa is still a common problem and there is a rise in the occurrence of otomycosis in latest years, especially in tropical and subtropical humid climates.

Author(s):  
Kassim Dekhil

 Objective: This study was aimed to identify the public pattern of presentation, influencing factors, and sort the fungal species, distribution of sex of patients with otomycosis.Results: The predominant complaints were pruritus and found in 76 patients (88.73%), discomfort and pain found in 62 patients (72.09%), aural fullness in 48 patients (55.81%), tinnitus in 34 patients (39.53%), hearing impairment in 50 cases (58.31%), ear discharge in 22 patients (25.58%), and most of the symptoms seen in 36 patients (68.14%). The results showed a total of eight fungal species belong to six different genera, namely, Aspergillus, Candida, Penicillium, Rhizopus, Alternaria, and Cephalosporium were isolated during this study. Among identified fungi, Aspergillus niger was found to be the most prevalent fungal species with 35.71% followed by Candida albicans (27.55%), Aspergillus flavus (10.20%), Aspergillus fumigatus (8.16), Penicillium digitatum (6.12%) and Cephalosporium species (4.08%), and Rhizopus species (5.1%), while Alternaria alternata had the lowest percentage (6.54%).Conclusion: Otomycosis/mycotic otitis externa is still a common problem and there is a rise in the occurrence of otomycosis in latest years, especially in tropical and subtropical humid climates.


Author(s):  
Kavitha N ◽  
Zehra Amtuz

Birds are potential vectors of some diseases as their droppings pose public health risk and cause illness. Dust containing dry faeces after inhaling can infects humans. The prevalence of fungi from the faeces of birds in wetlands has not been previously determined. Hence, the present study was carried out to isolate and identify different fungal species present in the droppings of spot-billed pelican, Pelecanus philippensis. Among the fungal species identified Aspergillus flavus, Aspergillus niger and Aspergillus fumigatus were found to be dominating.


2020 ◽  
Vol 18 ◽  
Author(s):  
Faheem Hadi ◽  
Tahir Maqbool ◽  
Sameera Khurshid ◽  
Aisha Nawaz ◽  
Saira Aftab ◽  
...  

Background: Fungal infections have always remain a problem and they are getting worse with passage of each year due to their resistance against available antibiotics. Natural ways of treatment with homoeopathic medicines made of mother tinctures of plants have no reported side effects and have been proved effective against many bacterial and fungal infections. Materials & Methods: Preparation of mother tinctures of plants Cressa cretica, Leptadenia pyrotechnica and Pulicaria crispa was done and used to evaluate the anti-fungal potential of these plants against potentially pathogenic fungal species like Aspergillus niger, Aspergillus flavus, Aspergillus ustus and Candida albicans by agar disc diffusion method. Each tincture was evaluated at 0.25 ml, 0.5 ml and 1 ml volume per disc and zone of inhibition was measured in millimetres and compared with commercial drug Fluconazole (2 mg/ml) which was used as standard. Results: The results showed that satisfactory anti-fungal activity of these plants in comparison to standard drug Fluconazole. The ratio of antifungal activity of Cressa cretica measured as the zone of growth inhibition of these cultures against Aspergillus niger, Aspergillus flavus, Aspergillus ustus and Candida albicans was 24 mm / 27 mm, 30mm / 28 mm, 23 mm/ 30 mm and 32 mm/ 30 mm respectively. In the case of Leptadenia pyrotechnica, the mother tincture’s antifungal activity was 32 mm / 27 mm, 30 mm / 28 mm, 17 mm/ 30 mm and 24 mm/ 30 mm. In case of our third plant Pulicaria crispa, antifungal activity came out to be 23 mm/ 27 mm, 26 mm/ 28 mm, 26 mm / 30 mm and 24 mm/ 30 mm. Conclusion: Our study proved that mother tincture of these plants can be a potential new therapy to treat fungal infections and has the potential to rule out fungal problems. Further research using mother tincture of these plants against other fungal species has the potential to prove them a safer and widespread anti-fungal homoeopathic medicine.


2019 ◽  
Vol 9 (3) ◽  
pp. 36-39
Author(s):  
D. Sivaranjani ◽  
P. Saranraj ◽  
M. Manigandan ◽  
K. Amala

Plectranthus amboinicus or locally known as bangun-bangun, is an indigenous vegetable which can be freshly eaten. However, the plant is unpopular among local people and being neglected. It has been reported to be traditionally used for medicine to cure common illnesses such as cough, stomachache, headache and skin infection. Based on the potential, a study was conducted to bioprospect the antimicrobial activity of the essential oil. Plectranthus amboinicus essential oil of methanol and chloroform extract was tested against nine bacteria and four fungi i.e., Escherichia coli, Proteus vulgaris, Pseudomonas aeruginosa, Shigella flexneri, Klebsiella pneumoniae, Enterococcus faecal, Staphylococcus aureus, Salmonella typhi, Bacillus cereus, Candida albicans, Aspergillus niger, Aspergillus fumigatus and Aspergillus flavus. The methanol extract of Plectranthus amboinicus showed the maximum antibacterial activity against the bacteria Klebsiella pneumoniae and fungi Candida albicans. The antimicrobial activity of Plectranthus amboinicus was more at 100 mg/ml concentration when compared to 50 mg/ml concentration. Comparatively, the Methanol extract of Plectranthus amboinicus exhibited maximum antimicrobial activity when compared to Chloroform extract. The Plectranthus amboinicus has showed good antimicrobial activity against most of the bacteria and only one fungal yeast Candida albicans. No zone of inhibition was recorded against other fungal isolates like Aspergillus niger, Aspergillus flavus, Aspergillus fumigatus and Negative DMSO control. Keywords: Plectranthus amboinicus, Bacteria, Fungi, Antimicrobial activity and Well diffusion assay.


2011 ◽  
Vol 35 (1) ◽  
pp. 167-173
Author(s):  
Rusol Muhammedi Al Bahran

The study included 100 samples collected from different locations of the homes were located in the area of Ali Saleh in Baghdad 6 species were isolated from fungi and the most common genus or species of fungi isolated were Aspergillus fumigatus by frequency ratio of 25.84%, and occurrence ratio of 23%, Penicilium by frequency ratio of 21.34%, and occurrence ratio of 19%, Mucor by frequency ratio 20.22%, and the occurrence ratio of 18%, Candida albicans by frequency ratio of 15.73%, the occurrence ratio of 14%, Rhizopus frequency ratio by 13.48%, the occurrence ratio of 12% and Aspergillus niger frequency ratio by 3.37% and the occurrence ratio of 3%. Then the sensitivity test of disinfectants were studied against fungi isolated by using three disinfectants Chloroxylenol known commercially by (Dettol), Chlorhexidine commercially known by (Hibitane) and Sodium hypochlorite commercially known by (Bleach), and a study for the effected of three concentrations of each disinfectant (5, 2.5, 1.25)%, and the use of statistical analysis (ANOVA) to contrast the differences and Dnken test to the variation in any disinfectant or the most efficient concentrations of other disinfectants were observed that concentrations of 5% was the most efficient of concentrations than (2.5%) and (1.25%). As the disinfectant Dettol was significantly the most efficient from Bleach and Hibitane.


Author(s):  
سعاد محمد خليفة أبوالغيث ◽  
أحلام القمودي محمد زعيط

استهدفت هذه الدراسة عزل بعض أنواع الفطريات من التربة الملوثة بالهيدروكربون بمصفاة الزاوية لتكرير النفط، حيث تم عزل وتعريف بعض الفطريات مثل Rhizopus, Aspergillus fumigatus, Aspergillus niger, Aspergillus flavus, Aspergillus nidulans وأوضحت نتائج هذه الدراسة أن تواجد وتنوع فطر Aspergillus قد تفوق معنويا مقارنة بتواجد وتنوع فطرRhizopus. كما تم في هذه الدراسة اختبار قدرة وكفاءة الفطريات المعزولة على النمو واستغلال المركبات الهيدروكربونية المتمثلة في زيت الحمادة وزيت الشرارة بتركيز 1% و3%، حيث أوضحت النتائج بأن جنس Rhizopus سجل أعلى معدل للنمو على الوسط الغذائي Malt Extract Agar، وسجل كلا من فطر A. fumigatus وفطر A. flavus معدّل النمو القطري أعلى معنويا من النمو القطري لفطرA. niger  وفطر A. nidulans. هذه المعدّلات العالية تدل على إمكانية استخدام الفطريات المعزولة في المعالجة البيولوجية للتربة الملوّثة بالنفط.


2007 ◽  
Vol 74 (1) ◽  
pp. 49-53
Author(s):  
M.O. Xavier ◽  
I.M. Madrid ◽  
A.R.M. Meinerz ◽  
M.B. Cleff ◽  
L.F.D. Schuch ◽  
...  

RESUMO A aspergilose é causada por fungos ubíquos e oportunistas do gênero Aspergillus, que liberam milhares de conídios no ar, contaminando o ambiente, sendo de extrema importância a utilização de filtros de ar e programas corretos de anti-sepsia e desinfecção para prevenção da enfermidade. Este estudo avaliou a eficácia “in vitro” dos agentes químicos, iodóforo, amônia quaternária e clorexidina, frente a isolados de Aspergillus fumigatus (8), Aspergillus niger (8), Aspergillus flavus (6) e Aspergillus terreus (1). Para o teste foram preparadas diluições sucessivas dos desinfetantes/antisépticos (log2) em caldo RPMI, e os inóculos foram ajustados até uma concentração final de 5 x 104 UFC/mL. Foi realizada a técnica de microdiluição em caldo de acordo com NCCLS M-38, adaptada para agentes químicos, com incubação das microplacas a 35° C em agitação constante. A leitura visual dos resultados foi realizada após 96 horas, e os isolados de Aspergillus spp. utilizados foram resistentes ao iodóforo nas concentrações testadas. A amônia quaternária e a clorexidina mostraram-se eficazes contra os isolados de Aspergillus spp., com exceção de um A. fumigatus e um A. terreus. Com estes resultados indica-se a utilização da amônia quaternária e da clorexidina na prevenção da aspergilose, questionando-se o uso de iodóforos para este fim.


2021 ◽  
Vol 29 (03) ◽  
Author(s):  
Nangial Bashir Ullah

Objective: To compare the antifungal activity of wild and cultivated Ficus carica Linn (common fig) leaves extract Materials and Methods: An experimental study was conducted in the department of Botany Islamia College Peshawar from June 2016 to December 2016 which was shaped in 2021 into a research study in the Department of Community Medicine, Khyber Medical College Peshawar. The agar tube dilution method was used for antifungal activity of the extracts. Results: Comparison of Zone of Inhibition (%) in both Aspergillus niger and Aspergillus fumigatus colonies revealed that cultivated species of Ficus carica Linn (common fig locally known as Anjeer ) had more antifungal property against both the fungal species (63% and higher compared that to wild species having maximum zone of inhibition of 54.54%) with the exception of wild plant extract in polar solvent such as chloroform which had high level of antifungal activity (61.53%) only against Aspegillus fumigatus. The experiment also revealed that extracts from both wild and cultivated Ficus carica Linn leaves in polar solvents such as methanol( also written and referred to methanolic in the article) and chloroform showed higher level of antifungal activity against both the fungal species compared to extract taken in non-polar solvents.   Conclusion: Extract from cultivated species of Ficus carica Linn had higher level of activity against both the fungal species i.e. Aspergillus niger and Aspergillus fumigatus, especially extract taken in polar solvents. Key words: Ficus carica Linn, antifungal, tube dilution, zone of inhibition   


1993 ◽  
Vol 56 (3) ◽  
pp. 246-248 ◽  
Author(s):  
V. SANCHIS ◽  
A. SANCLEMENTE ◽  
J. USALL ◽  
I. VIÑAS

The predominant fungal species present in 60 samples of barley collected in Spain were Alternaria alternata, Penicillium spp. and Aspergillus flavus. Of the 176 Alternaria isolates examined, 88.6% produced tenuazonic acid, 15.3% produced alternariol, and 9% produced alternariol monomethyl ether. Only 6% of the 190 isolates of A. flavus produced aflatoxin.


1971 ◽  
Vol 123 (3) ◽  
pp. 331-339 ◽  
Author(s):  
Ah Law ◽  
D. R. Threlfall ◽  
G. R. Whistance

1. Ten moulds and two yeasts were analysed for the presence of 2-polyprenylphenols, 2-polyprenyl(H2)phenols, 6-methoxy-2-polyprenylphenols, 6-methoxy-2-polyprenyl(H2)phenols, 6-methoxy-2-polyprenyl-1,4-benzoquinones, 6-methoxy-2-polyprenyl(H2)-1,4-benzoquinones, 5-demethoxyubiquinones, 5-demethoxyubiquinones(H2), ubiquinones and ubiquinones(H2). 2. The organisms were found to be of three types: (a) those that contained only ubiquinones (Aspergillus fumigatus and Penicillium brevi-compactum) or ubiquinones(H2) (Alternaria solani, Claviceps purpurae and Penicillium stipitatum); (b) those that contained 5-demethoxyubiquinones and ubiquinones (Agaricus campestris, Aspergillus niger, Phycomyces blakesleeanus, Rhodotorula glutinis and Saccharomyces cerevisiae) or 5-demethoxyubiquinones(H2) and ubiquinones(H2) (Aspergillus quadrilineatus and Neurospora crassa); (c) one that contained 2-decaprenyl(H2)phenol, 6-methoxy-2-decaprenyl(H2)phenol, 6-methoxy-2-decaprenyl(X-H2)-1,4-benzoquinone, 5-demethoxyubiquinone-10(X-H2) and ubiquinones(H2) (Aspergillus flavus). 3. Studies were made on the biosynthesis of ubiquinones and ubiquinones(H2) by Asp. flavus, Phyc. blakesleeanus and S. cerevisiae. These provided evidence that in Phyc. blakesleeanus 5-demethoxyubiquinone-9 is a precursor of ubiquinone-9 and that in S. cerevisiae 5-demethoxyubiquinone-6 is a precursor of ubiquinone-6. In addition they yielded results that may be interpreted as providing evidence that in Asp. flavus 6-methoxy-2-decaprenyl(X-H2)-1,4-benzoquinone and 5-demethoxyubiquinone-10(X-H2) are precursors of ubiquinone-10(X-H2).


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