scholarly journals PIPER CHABA EXTRACTS WITH ANTIBIOFILM ACTIVITY INFLUENCE ANTIHYPERGLYCEMIC AND ANTIHYPERLIPIDEMIC RESPONSES IN DIABETIC WISTER RATS

2021 â—½  
pp. 44-49
Author(s):  
SULTANA RAJIA â—½  
KHADIZA KHANAM â—½  
UMME FARHANA â—½  
SOHANUR RAHMAN â—½  
RASHIDUL HAQUE
Keyword(s):  
Ethyl Acetate â—½  
Therapeutic Potential â—½  
Low Density Lipoprotein â—½  
Fasting Blood Glucose â—½  
Intraperitoneal Administration â—½  
Density Lipoprotein â—½  
Antimicrobial Activities â—½  
Positive Control â—½  
Diabetic Rats â—½  
Antibiofilm Activity

Objectives: Piper chaba, native to South and Southeast Asia, has been traditionally used as a medicinal plant. Aim of this study was to evaluate the antihyperglycemic and antihyperlipidemic activities of P. chaba root extracts (RE) in streptozotocin (STZ)-induced diabetic rats along with its antimicrobial activity. Methods: Diabetes was induced in Wister rats through the intraperitoneal administration of STZ (50 mg/kg b.w.). Antidiabetic and antilipidemic activities of the RE (in methanol, ethanol, ethyl acetate and distilled water) were evaluated by administering oral dose (200 mg/kg b.w.) for 21 days. Metformin (12.1 mg/kg b.w.) was used as a positive control. Blood samples of rats were drawn by tail vein puncture and cardiac puncture to determine the fasting blood glucose (FBG) and serum level of total cholesterol (TC), triglycerides (TG), low-density lipoprotein (LDL), and high-density lipoprotein (HDL), respectively. Standard protocols were followed to determine the antimicrobial and antibiofilm activities against two different strains of bacteria. Results: Oral administration of P. chaba RE for 21 days resulted in a significant (p< 0.001) decrease in FBG and TC, TG, and LDL levels (p<0.001), when compared to the untreated diabetic rats. Significant (p<0.001) increase of HDL was observed when ethyl acetate and aqueous RE were administered. Out of four, two extracts showed varying antimicrobial activities, particularly against the gram-positive bacteria.  Conclusion: It became evident for the first time that P. chaba extracts possess antimicrobial activities and can serve as biochemical compounds with great alternative therapeutic potential in the management of diabetes and hypercholesterolemia.

scholarly journals Pleurotus tuber-regiumPolysaccharides Attenuate Hyperglycemia and Oxidative Stress in Experimental Diabetic Rats

10.1155/2012/856381 â—½  
2012 â—½  
Vol 2012 â—½  
pp. 1-8 â—½  
Author(s):  
Hui-Yu Huang â—½  
Mallikarjuna Korivi â—½  
Ying-Ying Chaing â—½  
Ting-Yi Chien â—½  
Ying-Chieh Tsai
Keyword(s):  
Oxidative Stress â—½  
Serum Insulin â—½  
Glycosylated Hemoglobin â—½  
Low Density Lipoprotein â—½  
Fasting Blood Glucose â—½  
Antioxidant Properties â—½  
Density Lipoprotein â—½  
Diabetic Rats â—½  
High Fat â—½  
And Oxidative Stress

Pleurotus tuber-regiumcontains polysaccharides that are responsible for pharmacological actions, and medicinal effects of these polysaccharides have not yet been studied in diabetic rats. We examined the antidiabetic, antihyperlipidemic, and antioxidant properties ofP. tuber-regiumpolysaccharides in experimental diabetic rats. Forty rats were equally assigned as diabetic high-fat (DHF) diet and polysaccharides treated DHF groups (DHF+1P, DHF+2P, and DHF+3P, 20 mg/kg bodyweight/8-week). Diabetes was induced by chronic low-dose streptozotocin injections and a high-fat diet to mimic type 2 diabetes. Polysaccharides (1P, 2P, and 3P) were extracted from three different strains ofP. tuber-regium. Fasting blood glucose and glycosylated hemoglobin (HbA1c) levels substantially decreased, while serum insulin levels were restored by polysaccharides treatment compared to DHF. Furthermore, plasma total cholesterol, triglycerides, and low-density lipoprotein levels were significantly(P<0.01)lower in polysaccharide groups. High-density lipoprotein levels were attenuated with polysaccharides against diabetes condition. Polysaccharides inhibited(P<0.01)the lipid peroxidation index (malondialdehyde), and restored superoxide dismutase and glutathione peroxidase activities in the liver of diabetic rats. The antihyperglycemic property of polysaccharides perhaps boosts the antioxidant system that attenuates oxidative stress. We emphasize thatP. tuber-regiumpolysaccharides can be considered as an alternative medicine to treat hyperglycemia and oxidative stress in diabetic rats.

scholarly journals Therapeutic Effects of 5,7-Dihydroxy-6-Oxoheptadecanoic Acid on Dysglycemia, Dyslipidemia, and Other Complications in Diabetic Rats

2020 â—½  
Vol 15 (7) â—½  
pp. 1934578X2093720
Author(s):  
Cuilan An â—½  
Lingling Wang â—½  
Yongli Liu â—½  
Emmanuel Ayobami Makinde â—½  
Huilian Li â—½  
...  
Keyword(s):  
Liver Function â—½  
Serum Insulin â—½  
Low Density Lipoprotein â—½  
Fasting Blood Glucose â—½  
Density Lipoprotein â—½  
Insulin Level â—½  
Diabetic Rats â—½  
The Body â—½  
Antioxidant Enzyme Activities â—½  
Therapeutic Effects

The current study aimed to investigate the therapeutic effects of 5,7-dihydroxy-6-oxoheptadecanoic acid (DHA) from Tiliacora triandra on rat models of type 2 diabetes mellitus (T2DM). T2DM was induced with a combination of high-fat diet/streptozotocin (HFD/STZ), and diabetic rats were treated with DHA (25 mg/kg) for 30 days. The body weight, fasting blood glucose (FBG), serum, and liver biochemical parameters, as well as histological evaluations of the liver and pancreas, were evaluated. Diabetic rats displayed a significant increase in FBG, serum lipid profiles (triglycerides, total cholesterol, and low-density lipoprotein cholesterol), liver function enzymes (aspartate transaminase, alkaline phosphatase, and alanine transaminase), creatinine, liver malondialdehyde (MDA), and myeloperoxidase (MPO) contents. Furthermore, insulin level and liver antioxidant enzyme activities (catalase [CAT], superoxide dismutase [SOD], and glutathione peroxidase [GSH-Px]) were significantly reduced in the diabetic rats. Whereas, treatment with DHA significantly reduced FBG, serum lipids, liver function enzymes, serum creatinine, liver MDA, and MPO contents. In addition, treatment with DHA significantly increased serum insulin level and liver SOD, CAT, and GSH-Px activities. In addition, DHA alleviated histopathological changes in the pancreas and liver caused by T2DM. These results portray the antidiabetic and antioxidative properties of DHA and can be considered as a potential treatment for T2DM.

scholarly journals Glycaemic, Lipidaemic and Antioxidant Profiles of Alloxan-Induced Diabetic Wistar Rats Treated with Glibenclamide and Aqueous Extract of Gongronema latifolium (Benth)

10.15835/nsb849857 â—½  
2016 â—½  
Vol 8 (4) â—½  
pp. 408-413
Author(s):  
Patrick Emeka ABA
Keyword(s):  
Wistar Rats â—½  
Combined Therapy â—½  
Low Density Lipoprotein â—½  
Fasting Blood Glucose â—½  
Density Lipoprotein â—½  
Study Groups â—½  
Oral Route â—½  
Diabetic Rats â—½  
Glucose Levels â—½  
Male Wistar Rats

The current study investigated the ameliorative effects of combined therapy of glibenclamide and G. latifolium (GL) on several biochemical parameters of alloxaized Wistar rats. Thirty adult male Wistar rats assigned into 5 groups of 6 rats each were used for the study. Groups 2-5 were intraperitoneally injected with 160 mg/kg of alloxan monohydrate and upon establishment of diabetes (Fasting Blood Glucose (FBG) ≥ 126 mg/dl) were treated with 10 ml/kg distilled water (DW), 2 mg/kg glibenclamide, 200 mg/kg GL and 2 mg/kg glibenclamide and 200 mg/kg GL respectively. Rats in group 1 were not made diabetic and served as normal control. All the treatments were realized through daily oral route using gastric tube, for 21 days. Results indicated that the treatment of diabetic rats with a combination of glibenclamide and GL significantly reduced the elevated glucose levels, cholesterol, triacylglycerol, low density lipoprotein and malondialdehyde levels, along with increases in the high density lipoprotein, glutathione values and catalase activities, when compared to diabetic untreated group. It was concluded that the combined therapy of glibenclamide and GL showed superior antihyperglycemic, hypolipidaemic and antioxidant effects compared to either of them used alone.

scholarly journals Antidiabetic and Antioxidant Properties ofTriticum aestivumin Streptozotocin-Induced Diabetic Rats

10.1155/2013/716073 â—½  
2013 â—½  
Vol 2013 â—½  
pp. 1-9 â—½  
Author(s):  
Yogesha Mohan â—½  
Grace Nirmala Jesuthankaraj â—½  
Narendhirakannan Ramasamy Thangavelu
Keyword(s):  
Triticum Aestivum â—½  
Body Weight â—½  
Glycosylated Hemoglobin â—½  
Low Density Lipoprotein â—½  
Fasting Blood Glucose â—½  
Density Lipoprotein â—½  
Diabetic Rats â—½  
Albino Rats â—½  
Low Density â—½  
Marker Enzyme

The antidiabetic and antioxidant potential ofTriticum aestivumwere evaluated by usingin vivomethods in normal and streptozotocin-induced diabetic rats. Diabetes was induced in the Wistar strain albino rats by injecting streptozotocin at a dose of 55 mg/kg body weight. Ethanolic extracts ofTriticum aestivumat doses of 100 mg/kg body weight were administered orally for 30 days. Various parameters were studied and the treatment group with the extract showed a significant increase in the liver glycogen and a significant decrease in fasting blood glucose, glycosylated hemoglobin levels, and serum marker enzyme levels. The total cholesterol and serum triglycerides levels, low density lipoprotein, and very low density lipoprotein were also significantly reduced and the high density lipoprotein level was significantly increased upon treatment with theTriticum aestivumethanol extract. A significant decrease in the levels of lipid peroxides, superoxide dismutase, and glutathione peroxidise and increase in the levels of vitamin E, catalase, and reduced glutathione were observed inTriticum aestivumtreated diabetic rats. Thus, from this study we conclude that ethanolic extract ofTriticum aestivumexhibited significant antihyperglycemic, hypolipidemic, and antioxidant activities in streptozotocin-induced diabetic rats.

scholarly journals Bilobalide Enhances AMPK Activity to Improve Liver Injury and Metabolic Disorders in STZ-Induced Diabetes in Immature Rats via Regulating HMGB1/TLR4/NF-κB Signaling Pathway

2021 â—½  
Vol 2021 â—½  
pp. 1-11
Author(s):  
Meng Zhao â—½  
Jianpin Qin â—½  
Wenting Shen â—½  
Aiping Wu
Keyword(s):  
Liver Injury â—½  
Serum Insulin â—½  
Low Density Lipoprotein â—½  
Fasting Blood Glucose â—½  
Density Lipoprotein â—½  
Blood Lipid â—½  
Diabetic Rats â—½  
Compound C â—½  
Group I â—½  
Immature Rats

This study was aimed at examining the effect and underlying mechanisms of bilobalide (BB) on hepatic injury in streptozotocin- (STZ-) induced diabetes mellitus (DM) in immature rats. Immature rats (one day old) were randomly divided into five groups: group I, control nondiabetic rats; group II, STZ-induced, untreated diabetic rats; groups III/IV/V, STZ-induced and BB-treated diabetic rats, which were intraperitoneally injected with BB (2.5 mg/kg, 5 mg/kg, or 10 mg/kg) after 3 days followed by STZ treatment. We observed that BB improved the histopathological changes and maintained normal glucose metabolism, blood lipid, and liver function indicators, such as fasting blood glucose, obesity index, HbA1c, HOMA-IR, fast serum insulin, adiponectin, total cholesterol (TC), triglyceride (TG), high-density lipoprotein (HDL), low-density lipoprotein (LDL), aspartate transaminase (AST), and alanine transaminase (ALT) in STZ-induced DM in immature rats by a biochemical analyzer or ELISA. Meanwhile, Western blot analysis showed that in STZ-induced DM immature rats, BB decreased the expression of apoptosis-related proteins Bax, cleaved caspase-3, and cleaved caspase-9 while enhancing the Bcl-2 expression; BB downregulated the expression of ACC related to fat anabolism, while upregulating the expression of CPT-1 related to fat catabolism. Strikingly, treatment with BB significantly increased the expression of AMPKα1 as well as inhibited HMGB1, TLR4, and p-P65 expression in hepatic tissues of immature DM rats. AMPK inhibitor (compound C, CC) cotreated with BB undermined the protective effect of BB on the liver injury. The results of the present study suggested BB may have a significant role in alleviating liver damage in the STZ-induced immature DM rats.

scholarly journals Hypoglycemic and Hypolipidemic Activities of Psychotria sylhetensis Hook in Alloxan-induced Type 2 Diabetic Rats

2019 â—½  
Vol 22 (1) â—½  
pp. 41-44
Author(s):  
Israt Jahan Bulbul â—½  
Umma Shafia Joty â—½  
Ridoy Ahamed â—½  
Mohammad Rashedul Haque â—½  
Mohammad A Rashid
Keyword(s):  
Glucose Level â—½  
Methanolic Extract â—½  
Low Density Lipoprotein â—½  
Intraperitoneal Administration â—½  
Density Lipoprotein â—½  
Pharmaceutical Journal â—½  
Diabetic Control â—½  
Diabetic Rats â—½  
Lipoprotein Cholesterol â—½  
Beneficial Effects

The current study was carried out to determine the hypoglycemic and hypolipidemic activities of methanolic extract P. silhetensis leaves in alloxan-induced diabetic rats. In this study diabetes was induced in rats by intraperitoneal administration of 150 mg/kg of alloxan. Animals were treated for one week with the plant extract at doses of 250 and 500 mg/kg body weight. Metformin (100 mg/kg b.w.) was used as standard for comparison. The anti-diabetes activity was examined by measuring blood glucose level at 0, 3, 5 and 7 days in diabetic induced animal models. Blood samples were collected at day eight of the treatment and analyzed for triglyceride (TG), total cholesterol (TC), high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL-C) after sacrifice. Alloxan-induced diabetic rats exhibited a significant decrease in plasma glucose level when treated with P. silhetensis leaf extract as compared to normal, diabetic control and standard rats. All tested lipid parameters were improved after the treatment with methanolic extract of P. silhetensis and metformin as compared to diabetic rats. The results of the present study showed that P. silhetensis possesses significant anti-diabetic properties and has beneficial effects on diabetic hyperlipidemia. Bangladesh Pharmaceutical Journal 22(1): 41-44, 2019

scholarly journals Antidiabetic and Antioxidative Effect of Jiang Tang Xiao Ke Granule in High-Fat Diet and Low-Dose Streptozotocin Induced Diabetic Rats

10.1155/2014/475192 â—½  
2014 â—½  
Vol 2014 â—½  
pp. 1-8 â—½  
Author(s):  
Dan-Dan Zhao â—½  
Na Yu â—½  
Xiao-Ke Li â—½  
Xin Fang â—½  
Qian-qian Mu â—½  
...  
Keyword(s):  
High Fat Diet â—½  
Low Density Lipoprotein â—½  
Herbal Medicines â—½  
Density Lipoprotein â—½  
Positive Control â—½  
Serum Glucose â—½  
Diabetic Rats â—½  
High Fat â—½  
Naturally Occurring â—½  
Alanine Aminotransferase Activity

Diabetes mellitus (DM), a kind of metabolic disease, is increasing over the last four decades in the world. The purpose of this study was to investigate the effect of Jiang Tang Xiao Ke (JTXK) granule, a naturally occurring ingredient from Chinese herbal medicines, on serum glucose, lipids, and oxidative stress in DM rats induced by high-fat diet and streptozotocin. JTXK granule 9 g/kg (based on crude herb equivalent) and pioglitazone 1.5 mg/kg (as a positive control for comparison) were orally administrated to DM rats for 4 weeks. Results showed that administration of JTXK granule reduced serum glucose, total cholesterol, triglyceride, and low density lipoprotein levels (by 12%, 33%, 57%, and 44%, resp.) but increased high-density lipoprotein level by 69%, compared with the drug-untreated DM rats. Serum malondialdehyde and nitric oxide levels were lowered (by 34% and 52%, resp.) associated with the elevation in serum superoxide dismutase levels (by 60%) after JTXK granule treatment. In addition, JTXK granule suppressed serum alanine aminotransferase activity (up to 50%) and alleviated pathological changes of pancreas and liver tissues in DM rats. The beneficial changes of pioglitazone on biomarkers were also found in DM rats. These findings suggested that JTXK granule may be an alternative medicine for the management of DM.

scholarly journals Hypoglycemic, antidyslipidemic, and antioxidant activities of methanol extract of Struchium sparganophora leaves in alloxan-induced oxidative stress-mediated diabetes in rats

2020 â—½  
Vol 6 (1) â—½  
Author(s):  
A. M. Adeosun â—½  
F. O. Asejeje â—½  
O. M. Ighodaro â—½  
B. A. Oluwole â—½  
O. A. Akinloye
Keyword(s):  
Oxidative Stress â—½  
Blood Glucose â—½  
Methanol Extract â—½  
Antioxidant Activities â—½  
Low Density Lipoprotein â—½  
Fasting Blood Glucose â—½  
Density Lipoprotein â—½  
Hdl Cholesterol â—½  
Diabetic Rats â—½  
Stress Markers

Abstract Background Diabetes mellitus is clinically underlined by hyperglycemia and dyslipidemia. In view of this, the current study assessed the glycemic and lipidemic control potentials of methanol extract of Sruchium sparganophora leaves (SPA) in the alloxan-induced diabetic model using male Wistar rats. Experimental diabetes was induced through a single intraperitoneal injection of 120 mg/kg freshly prepared alloxan. Thirty-six rats were randomly assigned into six groups of normoglycemic control, untreated diabetic group, and diabetic treated with (i) metformin (12 mg), (ii) metformin 12 mg + SPA 300 mg/kg, (iii) SPA 300 mg/kg, and (iv) SPA 600 mg/kg per os twice at 9.00 and 18.00 h daily for 10 days. Fasting blood glucose (FBG) level and markers of dyslipidemia, and oxidative stress markers were determined. Results SPA at selected doses decreased fasting blood glucose which was significantly (p < 0.05) raised by alloxan. Increase in plasma total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), and triglyceride (TG) concentrations and decrease in HDL-cholesterol (HDL-C) concentration (p < 0.05) caused by alloxan were significantly moderated by SPA at selected doses. Glutathione-s-transferase (GST), superoxide dismutase (SOD), and catalase (CAT) activities reduced by alloxan (p < 0.05) in both the liver and pancreas were reversed by SPA 300 and 600, and its combination with metformin. Decreased reduced glutathione (GSH) concentration in alloxan diabetic rats was also reversed by the extract, while the level of malondialdehyde (MDA) exacerbated by alloxan (p < 0.05) in the tissues was decreased by the extracts. Conclusion Struchium sparganophora possesses considerable antihyperglycemic, antidyslipidemic, and antioxidant potentials without compromising organ functionality.

scholarly journals Hypoglycaemic and hypolipidaemic effects of crude extracts and chromatographic fractions of Morinda morindoides root bark in diabetic rats

2012 â—½  
Vol 81 (3) â—½  
pp. 269-274 â—½  
Author(s):  
Johnny Olufemi Olukunle â—½  
Matthew Oluwole Abatan â—½  
Olubukola Tolulope Adenubi â—½  
Timothy Adeolu Amusan
Keyword(s):  
Low Density Lipoprotein â—½  
Density Lipoprotein â—½  
Positive Control â—½  
Diabetic Rats â—½  
Root Bark â—½  
Sorbitan Monooleate â—½  
Morinda Morindoides â—½  
Methanolic Extracts â—½  
Hypoglycaemic Activity â—½  
Accelerated Gradient

Hypoglycaemic and hypolipidaemic effects of different extracts and fractions of root bark from the plantMorinda morindoides(Baker) Milne-Redh of the familyRubiaceaewere evaluated in alloxan-induced diabetic rats. The aqueous and methanolic extracts were administered to 48 rats orally at a dose of 400 mg·kg-1for 21 days.Fractions (hydromethanol, hexane, chloroform and ethyl acetate) from bio-activity guided fractionation and chromatographic sub fractions (CsF) A-F from accelerated gradient chromatography were also evaluated in 45 rats for the hypoglycaemic activity at doses of 400 mg·kg-1, 200 mg·kg-1and 100 mg·kg-1of solvent fractions and (CsF) A-F, respectively. Glibenclamide was used as positive control. Polyoxyethylene sorbitan monooleate and distilled water administered to rats were used as negative controls. The dose of 400 mg·kg-1of aqueous and methanolic extracts and 100 mg·kg-1of chloroformCsFB ofMorinda morindoidescaused (62.8%, 56% and 74%, respectively) reductions in blood glucose level (BGL). The aqueous extract caused significant (P< 0.05) decreases in the values of serum cholesterol (133.48 ± 1.1) mg·dl-1, low density lipoprotein (66.38 ± 2.5) mg·dl-1and significant (P< 0.05) increase in the value of high density lipoprotein (51.03 ± 3.0) mg·dl-1when compared to the control. These results confirm the folkloric claim of the hypoglycaemic and hypolipidaemic activities ofMorinda morindoidesroot bark.

scholarly journals Evidence-Based Critical Evaluation of Glycemic Potential ofCynodon dactylon

10.1093/ecam/nem044 â—½  
2008 â—½  
Vol 5 (4) â—½  
pp. 415-420 â—½  
Author(s):  
Santosh Kumar Singh â—½  
Prashant Kumar Rai â—½  
Dolly Jaiswal â—½  
Geeta Watal
Keyword(s):  
Blood Glucose â—½  
Cynodon Dactylon â—½  
Low Density Lipoprotein â—½  
Fasting Blood Glucose â—½  
Critical Evaluation â—½  
Density Lipoprotein â—½  
Ethanolic Extract â—½  
Tolerance Test â—½  
Diabetic Rats â—½  
Sd Rats

The present study is an extension of our previous work carried out onCynodon dactylon. This study deals with the critical evaluation of glycemic potential of ethanolic extract of defattedC. dactylon. The doses of 250, 500 and 750 mg kg−1bw of the extract were administered orally to normal as well as Streptozotocin-induced diabetic rats to study its glycemic potential. The effect of repeated oral administration of the same doses of ethanolic extract was also studied on serum lipid profile of severely diabetic (SD) rats. The dose of 500 mg kg−1bw was identified as the most effective dose as it lowered the blood glucose levels of normal by 42.12% and of diabetic by 43.42% during fasting blood glucose (FBG) and glucose tolerance test respectively. The SD rats were also treated daily with this identified dose of 500 mg kg−1bw for 2 weeks and a significant reduction of 56.34% was observed in FBG level. Total cholesterol, low density lipoprotein and triglyceride levels were also decreased by 32.94, 64.06 and 48.46% respectively in SD rats whereas, cardioprotective high density lipoprotein increased by 16.45%. The reduced urine sugar level and increased body weight are additional advantages. These evidences clearly indicate that the ethanolic extract of defattedC. dactylonhas high antidiabetic potential along with good hypolipidemic profile.

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