Expression profiles of long noncoding RNAs in cutaneous squamous cell carcinoma

The early onset and rapid progression of cutaneous squamous cell carcinoma (cSCC) leads to high mortality rates in individuals with recessive dystrophic epidermolysis bullosa (RDEB). Currently, the molecular mechanisms underlying cSCC development in RDEB are not well understood and there are limited therapeutic options. RDEB-cSCC arises through the accumulation of genetic mutations; however, previous work analyzing gene expression profiles have not been able to explain its aggressive nature. Therefore, we generated a model to study RDEB-cSCC development using cellular reprograming and re-differentiation technology. We compared RDEB-cSCC to cSCC that were first reprogrammed into induced pluripotent stem cells (RDEB-cSCC-iPSC) and then differentiated back to keratinocytes (RDEB-cSCC-iKC). The RDEB-cSCC-iKC cell population had reduced proliferative capacities in vitro and in vivo, suggesting that reprogramming and re-differentiation leads to functional changes. Finally, we performed RNA-seq analysis for RDEB-cSCC, RDEB-cSCC-iPSC, and RDEB-cSCC-iKC and identified different gene expression signatures between these cell populations. Taken together, this cell culture model offers a valuable tool to study cSCC and provides a novel way to identify potential therapeutic targets for RDEB-cSCC.

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Gene Expression Profiling Of Perineural Invasion In Head And Neck Cutaneous Squamous Cell Carcinoma

10.21203/rs.3.rs-290025/v1 ◽

2021 ◽

Author(s):

Timothy J. Eviston ◽

Elahe Minaei ◽

Simon A. Mueller ◽

Navid Ahmadi ◽

Bruce Ashford ◽

...

Keyword(s):

Gene Expression ◽

Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Head And Neck ◽

Expression Analysis ◽

Squamous Cell ◽

Perineural Invasion ◽

Expression Profiles ◽

Cutaneous Squamous Cell Carcinoma ◽

Mesenchymal Transition

Abstract Perineural invasion (PNI) is frequently associated with aggressive clinical behaviour in head and neck cutaneous squamous cell carcinoma (HNcSCC) leading to local recurrence and treatment failure. This study evaluates the gene expression profiles of HNcSCC with PNI using a differential expression analysis approach and constructs a tailored gene panel for sensitivity and specificity analysis. 45 cases of HNcSCC were stratified into three groups (Extensive, Focal and Non PNI) based on predefined clinicopathological criteria. Here we show HNcSCC with extensive PNI demonstrates significant up- and down-regulation of 144 genes associated with extracellular matrix interactions, epithelial to mesenchymal transition, cell adhesion, cellular motility, angiogenesis, and cellular differentiation. Gene expression of focal and non PNI cohorts were indistinguishable and were combined for further analyses. There is clinicopathological correlation between gene expression analysis findings and disease behaviour and a tailored panel of 10 genes was able to identify extensive PNI with 96% sensitivity and 95% specificity.

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Identification of long noncoding RNAs for the detection of early stage lung squamous cell carcinoma by microarray analysis

Oncotarget ◽

10.18632/oncotarget.14522 ◽

2017 ◽

Vol 8 (8) ◽

pp. 13329-13337 ◽

Cited By ~ 6

Author(s):

Zule Cheng ◽

Yanan Bai ◽

Ping Wang ◽

Zhenhua Wu ◽

Lin Zhou ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Microarray Analysis ◽

Squamous Cell ◽

Early Stage ◽

Noncoding Rnas ◽

Lung Squamous Cell Carcinoma ◽

Long Noncoding Rnas

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RNA sequencing of exosomes revealed differentially expressed long noncoding RNAs in early-stage esophageal squamous cell carcinoma and benign esophagitis

Epigenomics ◽

10.2217/epi-2019-0371 ◽

2020 ◽

Vol 12 (6) ◽

pp. 525-541

Author(s):

Li Tian ◽

Lin Yang ◽

Wenjing Zheng ◽

Yinqing Hu ◽

Peikun Ding ◽

...

Keyword(s):

Squamous Cell Carcinoma ◽

Esophageal Squamous Cell Carcinoma ◽

Cell Carcinoma ◽

Squamous Cell ◽

Early Stage ◽

Noncoding Rnas ◽

Disease Status ◽

Long Noncoding Rnas ◽

Differentially Expressed ◽

Loss Of Function

Aim: To explore the roles of exosomal long noncoding RNAs (lncRNAs) in early-stage esophageal squamous cell carcinoma (ESCC) and benign esophagitis. Materials & methods: Exosomal lncRNAs were analyzed using RNA-seq and validated by quantitative real-time PCR, loss-of-function, co-culture and RNA pulldown assays. Results: Exosomal lncRNAs displayed tighter tissue-specificity, higher expression level and lower splicing efficiency than that of mRNAs. A total of 152 exosomal lncRNAs were differentially expressed between ESCC and controls. A total of 124 exosomal lncRNAs were dysregulated between ESCC and esophagitis. Knockdown of 13 ESCC-associated lncRNAs modified proliferation, migration, and apoptosis of ESCC cells. A novel lncRNA RP5-1092A11.2 was highly expressed in ESCC-derived exosomes, ESCC cells and tumor tissues. Exosomes released from RP5-1092A11.2-knockdown cells inhibited ESCC cell proliferation. Conclusion: Dysregulated exosomal lncRNAs were functionally associated with different disease status in esophagus.

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