scholarly journals IN VITRO FREE RADICAL SCAVENGING ACTIVITY OF HYDROETHANOLIC LEAF EXTRACT OF IXORA MACROTHYRSA (TEJISM. AND BINN.)

2018 ◽  
Vol 12 (04) ◽  
Author(s):  
Dr. D. Victor Arokia Doss
Keyword(s):  
Free Radical ◽  
Leaf Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity

Evaluation of In-vitro Antioxidant Potential and Screening, Characterization of Phytochemicals Using hydroethanolic leaf Extract of Caesalpinia Pulcherrima

2020 ◽  
Vol 09 ◽  
Author(s):  
Akila Ilangovan ◽  
Raghu V ◽  
Venugopal R ◽  
Vijay N ◽  
Yellasamy Y ◽  
...  
Keyword(s):  
Free Radical ◽  
Functional Groups ◽  
Leaf Extract ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Phytochemical Analysis ◽  
Scavenging Activity

Introduction: Novel Therapeutic Phytochemical studies are based on the use of plants in the production of drugs. This present study was carried out to the screening of phytochemicals and quantitative analysis, to assess In-vitro Antioxidant activities of the hydroethanolic leaf extract of Caesalpinia pulcherrima. FT-IR and GC-MS analysis of hydroethanolic leaf extract of Caesalpinia pulcherrima. Methods: The investigation of qualitative and determination of quantitative phytochemical analysis were performed using standard procedures. The total alkaloid, flavonoid, phenols, and tannin content were determined spectrophotometrically. DPPH (2,2-Diphenyl-1-picrylhydrazyl) free radical scavenging activity, ABTS (2,2’-azino-bis (3-ethylbenzothiazoline-6- sulfonic acid) free radical scavenging activity of hydroethanolic leaf extract of Caesalpinia pulcherrima was estimated by standard protocol. The identification of functional groups using plant leaf extracts by FTIR and GCMS. Results: The hydroethanolic leaf extract of Caesalpinia pulcherrima gives positive results for alkaloids, flavonoids, phenols, carbohydrates, tannins, amino acids, and proteins. The content of total alkaloids, flavonoids, phenols, and tannins varies from 8.4 AE/g, 3.3 CE/g, 2.9, and 3.2 GAE/g respectively. The antioxidant assay and IC50 values were found to be DPPH (43.3µg/ml), ABTS (46.1µg/ml), and ascorbic acid (41µg/ml), respectively and concentration ranging from 20 to 100µgml. Functional groups were also identified using the extract by FTIR and GCMS. Conclusions: From this work, it can be concluded that the presence of Phyto-component makes the plant useful in the treatment of various diseases. As such, it has been found that the hydroethanolic leaf extract of the plant contains more components and is beneficial for further research.

scholarly journals Antioxidant & analgesic activities of leaves of Panlata (Derris trifoliate): In vitro investigation

2012 ◽  
Vol 1 (2) ◽  
Author(s):  
Md Raihan Sarkar ◽  
Moynul Hasan ◽  
Md Sariful Islam Howlader ◽  
Mohammad Saydur Rahman ◽  
Shubhra Kanti Dey
Keyword(s):  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Immersion Method ◽  
Standard Drug ◽  
In Vitro Investigation

In the present study, the antioxidant and analgesic potential of the ethanolic extract of the leaves of Derris trifoliata was evaluated. The free radical scavenging activity of the crude extract on the stable radical 1, 1-diphenyl-2-picrylhydrazyl (DPPH) was determined by comparing the DPPH inhibitory capacity of the extract. In the quantitative assay, Derris trifoliata extract displayed a free radical scavenging activity in the DPPH assay (IC50 = 19 ?g/ml) which is comparable to that of ascorbic acid (IC50 = 7.80 ?g/ml), a well-known standard antioxidant. The analgesic responses of the given samples of extracts were evaluated using the Tail immersion method. In the analgesic activity test, extract at dose of 200 mg/kg and 400 mg/kg exhibited significant (P<0.05 and P<0.001 respectively) inhibition of pain by 166.82 and 184.95 after 120 and 180 minutes respectively while the standard drug Diclofenac Na inhibition was found to be 217.67 after 180 minutes at a dose of 25 mg/kg body weight DOI: http://dx.doi.org/10.3329/ijpls.v1i2.12951 International Journal of Pharmaceutical and Life Sciences Vol.1(2) 2012

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