scholarly journals Comparison of polyhedrin partial sequence of NPV variants pathogenic to tea pests (Geometridae) in India and their phylogeny

2019 ◽  
Vol 40 (1) ◽  
pp. 09-16
Author(s):  
B. Ghosh ◽  
◽  
A. Mukhopadhyay ◽  
M. Bahadur ◽  
◽  
...  
Keyword(s):  
Partial Sequence ◽  
Tea Pests

scholarly journals A PARTIAL SEQUENCE OF AMINO ACIDS IN PERFORMIC ACID-OXIDIZED VASOPRESSIN

1954 ◽  
Vol 206 (1) ◽  
pp. 353-360
Author(s):  
Edwin A. Popenoe ◽  
Vincent du Vigneaud
Keyword(s):  
Amino Acids ◽  
Performic Acid ◽  
Partial Sequence

scholarly journals Yeast Species Associated with Orange Juice: Evaluation of Different Identification Methods

2002 ◽  
Vol 68 (4) ◽  
pp. 1955-1961 ◽  
Author(s):  
Covadonga R. Arias ◽  
Jacqueline K. Burns ◽  
Lorrie M. Friedrich ◽  
Renee M. Goodrich ◽  
Mickey E. Parish
Keyword(s):  
Orange Juice ◽  
Restriction Pattern ◽  
Partial Sequence ◽  
Rrna Gene ◽  
Correct Identification ◽  
Internal Transcribed Spacer Region ◽  
Accurate Identification ◽  
Hanseniaspora Uvarum ◽  
Classical Methodology ◽  
26S Rrna

ABSTRACT Five different methods were used to identify yeast isolates from a variety of citrus juice sources. A total of 99 strains, including reference strains, were identified using a partial sequence of the 26S rRNA gene, restriction pattern analysis of the internal transcribed spacer region (5.8S-ITS), classical methodology, the RapID Yeast Plus system, and API 20C AUX. Twenty-three different species were identified representing 11 different genera. Distribution of the species was considerably different depending on the type of sample. Fourteen different species were identified from pasteurized single-strength orange juice that had been contaminated after pasteurization (PSOJ), while only six species were isolated from fresh-squeezed, unpasteurized orange juice (FSOJ). Among PSOJ isolates, Candida intermedia and Candida parapsilosis were the predominant species. Hanseniaspora occidentalis and Hanseniaspora uvarum represented up to 73% of total FSOJ isolates. Partial sequence of the 26S rRNA gene yielded the best results in terms of correct identification, followed by classical techniques and 5.8S-ITS analysis. The commercial identification kits RapID Yeast Plus system and API 20C AUX were able to correctly identify only 35 and 13% of the isolates, respectively. Six new 5.8S-ITS profiles were described, corresponding to Clavispora lusitaniae, Geotrichum citri-aurantii, H. occidentalis, H. vineae, Pichia fermentans, and Saccharomycopsis crataegensis. With the addition of these new profiles to the existing database, the use of 5.8S-ITS sequence became the best tool for rapid and accurate identification of yeast isolates from orange juice.

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