scholarly journals Intrinsically magnetic susceptibility in human blood and its impact on cell separation: Non-classical and intermediate monocytes have the strongest magnetic behavior in fresh human blood

2021 ◽  
Author(s):  
Jenifer Gómez-Pastora ◽  
James Kim ◽  
Victor Multanen ◽  
Mitchell Weigand ◽  
Nicole Walters ◽  
...  
Keyword(s):  
Magnetic Properties ◽  
Red Blood Cells ◽  
Human Blood ◽  
Blood Cells ◽  
Cell Tracking ◽  
Magnetic Behavior ◽  
Immunomagnetic Separation ◽  
Iron Storage ◽  
Cell Tracking Velocimetry ◽  
Classical Monocytes

The presence of iron in circulating monocytes is well known as they play an essential role in iron recycling. It has been demonstrated that the iron content of blood cells can be measured through their magnetic behavior; however, the magnetic properties of different monocyte subtypes remain unknown. In this study, we report for the first time, the magnetic behavior of classical, intermediate and non-classical monocytes, which is related to their iron storage capacity. The magnetic properties of monocytes were compared to other blood cells, such as lymphocytes and red blood cells in the oxyhemoglobin and methemoglobin states, and a cancer cell type. For this analysis, we used an instrument referred to as Cell Tracking Velocimetry (CTV), which quantitatively characterizes the magnetic behavior of biological entities. Our results demonstrate that significant fractions of the intermediate and non-classical monocytes have high magnetophoretic mobilities, equivalent to methemoglobin red blood cells and higher than the classical subset, suggesting their higher iron storage capacities. Moreover, our findings have implications for the immunomagnetic separation industry; we demonstrate that negative magnetic isolation techniques for recovering monocytes from blood should be used with caution, as it is possible to lose magnetic monocytes when using this technique.

scholarly journals The Growth of Staphylococcus aureus in the blood agar plate media of sheep blood and human blood groups A, B, AB, and O

2019 ◽  
Vol 8 (1) ◽  
pp. 1-7
Author(s):  
Dora Dayu Rahma Turista ◽  
Eka Puspitasari
Keyword(s):  
Health Education ◽  
Red Blood Cells ◽  
Human Blood ◽  
Blood Cells ◽  
Pathogenic Bacteria ◽  
Agar Plate ◽  
Blood Groups ◽  
Blood Agar Plate ◽  
Sheep Blood ◽  
Randomized Design

BAP media is a medium used to distinguish pathogenic bacteria based on their hemolytic power on red blood cells. Staphyllococcus aureus is a bacterium that is able to emolate red blood cells with 3 types of hemolysis, namely α, β, γ, and δ. Usually BAP media is made by adding 5-10% sheep blood. Making BAP media using sheep blood has become a problem for several laboratories today, including health education laboratories. This is because the health education laboratory does not yet have a sheep farm, so it has not been able to procure sheep blood. The use of human blood as a substitute for sheep blood in making BAP media may be a solution, but it is not yet known whether there are differences in the growth and hemolysis of S. aureus bacteria on BAP media in sheep's blood and human blood. This research is an experimental study with a completely randomized design (CRD) of 3 replications which aims to determine whether there are differences in growth and hemolysis of bacteria S. aureus in BAP media of sheep blood and human blood groups A, B, AB, and O. The results showed that S. aureus bacteria could grow and show hemolysis in BAP media in sheep blood and human blood in groups A, B, AB, and O. The results of subsequent studies analyzed ANOVA using the software spss for windows with a significant level of 0.05. From the results of research and data analysis it can be concluded that S. aureus bacteria can grow and show hemolysis in BAP media of sheep blood and human blood groups A, B, AB and O, but there are significant differences in the number of S. aureus bacteria colonies grown in BAP media of sheep's blood and human blood groups A, B, AB and O.

scholarly journals Merocyanine 540-sensitized photoinactivation of human erythrocytes parasitized by Plasmodium falciparum

Blood ◽  
1992 ◽  
Vol 80 (1) ◽  
pp. 21-24 ◽  
Author(s):  
OM Smith ◽  
SA Dolan ◽  
JA Dvorak ◽  
TE Wellems ◽  
F Sieber
Keyword(s):  
Stem Cells ◽  
Plasmodium Falciparum ◽  
Hematopoietic Stem Cells ◽  
Red Blood Cells ◽  
Human Blood ◽  
Blood Cells ◽  
Potential Usefulness ◽  
Hematopoietic Stem ◽  
Infected Blood ◽  
Merocyanine 540

The purpose of this study was to evaluate the photosensitizing dye merocyanine 540 (MC540) as a means for extracorporeal purging of Plasmodium falciparum-infected erythrocytes from human blood. Parasitized red blood cells bound more dye than nonparasitized cells, and exposure to MC540 and light under conditions that are relatively well tolerated by normal erythrocytes and normal pluripotent hematopoietic stem cells reduced the concentration of parasitized cells by as much as 1,000-fold. Cells parasitized by the chloroquine- sensitive HB3 clone and the chloroquine-resistant Dd2 clone of P falciparum were equally susceptible to MC540-sensitized photolysis. These data suggest the potential usefulness of MC540 in the purging of P falciparum-infected blood.

scholarly journals Plasma skimming efficiency of human blood in the spiral groove bearing of a centrifugal blood pump

2020 ◽  
Author(s):  
Daisuke Sakota ◽  
Kazuki Kondo ◽  
Ryo Kosaka ◽  
Masahiro Nishida ◽  
Osamu Maruyama
Keyword(s):  
Red Blood Cells ◽  
Human Blood ◽  
Blood Cells ◽  
High Speed ◽  
Blood Pump ◽  
Spiral Groove ◽  
Centrifugal Blood Pump ◽  
Plasma Skimming ◽  
Ridge Area ◽  
Spiral Groove Bearing

Abstract This work investigates the plasma skimming effect in a spiral groove bearing within a hydrodynamically levitated centrifugal blood pump when working with human blood having a hematocrit value from 0 to 40%. The present study assessed the evaluation based on a method that clarified the limitations associated with such assessments. Human blood was circulated in a closed-loop circuit via a pump operating at 4000 rpm at a flow rate of 5 L/min. Red blood cells flowing through a ridge area of the bearing were directly observed using a high-speed microscope. The hematocrit value in the ridge area was calculated using the mean corpuscular volume, the bearing gap, the cross-sectional area of a red blood cell, and the occupancy of red blood cells. The latter value was obtained from photographic images by dividing the number of pixels showing red blood cells in the evaluation area by the total number of pixels in this area. The plasma skimming efficiency was calculated as the extent to which the hematocrit of the working blood was reduced in the ridge area. For the hematocrit in the circuit from 0 to 40%, the plasma skimming efficiency was approximately 90%, meaning that the hematocrit in the ridge area became 10% as compared to that in the circuit. For a hematocrit of 20% and over, red blood cells almost completely occupied the ridge. Thus, a valid assessment of plasma skimming was only possible when the hematocrit was less than 20%.

Assessment of prion reduction filters in decreasing infectivity of ultracentrifuged 263K scrapie-infected brain homogenates in “spiked” human blood and red blood cells

Transfusion ◽  
2013 ◽  
Vol 54 (4) ◽  
pp. 990-995 ◽  
Author(s):  
Franco Cardone ◽  
Samuel Sowemimo-Coker ◽  
Hanin Abdel-Haq ◽  
Marco Sbriccoli ◽  
Silvia Graziano ◽  
...  
Keyword(s):  
Red Blood Cells ◽  
Human Blood ◽  
Blood Cells

Adsorption of Carbon-14 Dextran to Human Blood Platelets and Red Blood Cells, in Vitro

Vox Sanguinis ◽  
1957 ◽  
Vol 2 (2) ◽  
pp. 104-109 ◽  
Author(s):  
S. ROTHMAN ◽  
E. ADELSON ◽  
A. SCHWEBEL ◽  
R. D. LANGDELL
Keyword(s):  
Red Blood Cells ◽  
Human Blood ◽  
Blood Cells ◽  
Blood Platelets ◽  
Carbon 14 ◽  
Human Blood Platelets

scholarly journals Generation of transgenic mice with antithetical KEL1 and KEL2 human blood group antigens on red blood cells

Transfusion ◽  
2012 ◽  
Vol 52 (12) ◽  
pp. 2620-2630 ◽  
Author(s):  
Nicole H. Smith ◽  
Kate L. Henry ◽  
Chantel M. Cadwell ◽  
Ashley Bennett ◽  
Jeanne E. Hendrickson ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Red Blood Cells ◽  
Blood Group ◽  
Human Blood ◽  
Blood Cells ◽  
Blood Group Antigens ◽  
Human Blood Group

scholarly journals ‘Synthetic Blood’: Entangling Politics and Biology

2019 ◽  
Vol 25 (2) ◽  
pp. 28-55 ◽  
Author(s):  
Julie Kent ◽  
Darian Meacham
Keyword(s):  
Supply Chain ◽  
Synthetic Biology ◽  
Red Blood Cells ◽  
Human Blood ◽  
Blood Cells ◽  
Unmet Needs ◽  
Blood Donation ◽  
Ex Vivo ◽  
The Gift ◽  
Ex Vivo Culture

It is increasingly suggested that shortages in the supply chain for human blood could be met by the development of techniques to manufacture human blood ex vivo. These techniques fall broadly under the umbrella of synthetic biology. We examine the biopolitical context surrounding the ex vivo culture of red blood cells through the linked concepts of alienation, immunity, bio-value and biosecuritization. We engage with diverse meanings of synthetic blood, and questions about how the discourses of biosecurity and privatization of risk are linked to claims that the technology will address unmet needs and promote social justice. Through our discussion we contrast communitarian ideas that culturing red blood cells ‘extends the gift’ of adult blood donation with understandings of the immunitary logics that underpin the cord-blood economy.

A study of the deformability of red blood cells of a teleost fish, the yellowtail (Seriola quinqueradiata), and a comparison with human erythrocytes

1982 ◽  
Vol 96 (1) ◽  
pp. 209-220
Author(s):  
G. M. Hughes ◽  
Y. Kikuchi ◽  
H. Watari
Keyword(s):  
Blood Flow ◽  
Red Blood Cells ◽  
Human Blood ◽  
Blood Cells ◽  
Blood Flow Rate ◽  
Blood Flows ◽  
Human Red Blood Cells ◽  
Seriola Quinqueradiata ◽  
Flow Through ◽  
Environmental Temperatures

The blood of a carangid fish, the yellowtail (Seriola quinqueradiata) has been studied with particular reference to the deformability properties of the red blood cells. The rate at which blood flows through a Nuclepore filter containing 5 micrometers pores has been determined under the same conditions that have been used with human blood. Marked differences were found in the flow of yellowtail blood which depended on the particular way in which the blood had been sampled. Such differences seem to be due to a sensitivity of fish red blood cells to their environmental conditions. Blood flow through filters is temperature-dependent, the rate increasing with a rise in temperature. Measurements made at 37 degrees C gave values which were similar to those normally obtained for human red blood cells, in spite of their greater dimensions (10.4 × 6.8 × 3.4 micrometers), and nucleated nature. It was also found that the blood flow rate of human blood was slower than that of yellowtail blood when measured at the normal environmental temperatures (15 degrees C) for these fish.

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