scholarly journals Palynomorphological study of Dianthus petraeus waldst. et kit. (Caryophyllaceae)

2015 ◽  
Vol 67 (3) ◽  
pp. 973-980 ◽  
Author(s):  
Marina Macukanovic-Jocic ◽  
Snezana Jaric ◽  
Mica Mladenovic
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
Taxonomic Status ◽  
The Balkans ◽  
The Mean ◽  
Pollen Diameter ◽  
Scanning Electron

The pollen morphology of Dianthus petraeus (Caryophyllaceae), which is endemic to the Balkans, has been examined by both light and scanning electron microscopy in order to provide taxonomically valuable characters that might be used in the classification of the Caryophyllaceae, as well as to contribute to the pollen atlas of Serbian apiflora. The pollen grains of D. petraeus are radially symmetrical, apolar, spheroidal and medium-sized. The mean of the pollen diameter is 45.5?1.4 ?m. The sculpturing pattern of exine is microechinate-microperforate, and the tectum is covered with minute echini averaging 0.8?0.1 ?m in length, more or less evenly distributed between the perforations. The microechini average number per sample area of 5 ?m x 5 ?m is 3.2?0.6. In view of the number, position and type of the apertures, the grains are polypantoporate, with about 13 operculate pores, averaging 6.4?0.7 ?m in diameter. Each operculum is covered with 4-6 conical spinules, which are longer than those on the exine surface. The mean interporal distance is 13.3?1.3 ?m. The obtained palynological results provide information that contribute towards a better understanding of the taxonomic status of D. petraeus.

scholarly journals Pollen morphology of Iranian Dracocephalum L. (Lamiaceae) and its taxonomic significance

2015 ◽  
Vol 22 (2) ◽  
pp. 99-110 ◽  
Author(s):  
Maryam Naderifar ◽  
Ali Sonboli ◽  
Abbas Gholipour
Keyword(s):  
Electron Microscopy ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
Polar View ◽  
Taxonomic Significance ◽  
Infrageneric Classification ◽  
Exine Ornamentation ◽  
Plant Taxon ◽  
Scanning Electron

Pollen morphology of 11 Iranian Dracocephalum L. species was investigated using light and scanning electron microscopy to evaluate their taxonomic significance for the infrageneric classification of the genus. Pollen grains of all examined taxa were isopolar, hexacolpate, circular in polar view and spheroidal to prolate in equatorial view (P/E = 1.0?2.0). The smallest pollen grains were observed in D. aucheri (P = 29.7 ?m, E = 22.6 ?m), while the largest pollen was found in D. lindbergii (P = 45.1 ?m, E = 33.7 ?m). The highest and lowest apocolpium index (AI) were measured in D. aucheri (AI = 0.27) and D. surmandinum (AI = 0.08), respectively. Colpus membrane was egranulate in all examined species except for D. multicaule and D. ghahremanii. The main exine ornamentation type was characterized as bireticulate including five different subtypes. The results revealed that the exine ornamentation is a diagnostic character useful for the classification of Dracocephalum.Bangladesh J. Plant Taxon. 22(2): 99-110, 2015 (December)

scholarly journals Pollen morphology of the genus Eremanthus Less. (Vernonieae, Asteraceae)

2012 ◽  
Vol 26 (1) ◽  
pp. 46-57 ◽  
Author(s):  
BenoÎt Loeuille ◽  
Raquel Maria Batista Souza-Souza ◽  
Vanessa Holanda Righetti Abreu ◽  
Cláudia Barbieri Ferreira Mendonça ◽  
Vania Gonçalves-Esteves
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pollen Morphology ◽  
Additional Data ◽  
Phylogenetic Reconstruction ◽  
Pollen Grains ◽  
Prolate Spheroidal ◽  
Oblate Spheroidal ◽  
Quantitative Characters ◽  
Scanning Electron

In order to evaluate the significance of the pollen morphology for generic and infrageneric taxonomy of the genus Eremanthus (Vernonieae, Asteraceae), and to provide additional data for its phylogenetic reconstruction, the pollen of 20 of the 23 species of the genus was examined using light and scanning electron microscopy. Acetolysed pollen grains were measured, described, and illustrated using light microscopy, while non-acetolysed pollen grains were analyzed using scanning electron microscopy. Pollen grains of these species are isopolar, oblate-spheroidal in most of the species, more rarely prolate spheroidal or suboblate, subtriangular amb, tricolporate and subechinolophate. The variation among quantitative characters does not correlate with the macromorphological subdivision of the genus or with the generic or specific limits.

scholarly journals Pollen morphology of Ukrainian species of the genus Pedicularis L. (Orobanchaceae Vent.)

2012 ◽  
Vol 24 (1) ◽  
pp. 5-13 ◽  
Author(s):  
Olena M. Peregrym ◽  
Zoya M. Tsymbalyuk ◽  
Sergei L. Mosyakin
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
Taxonomic Revision ◽  
Palynological Data ◽  
First Time ◽  
Scanning Electron

Abstract Pollen grains of 10 species of Pedicularis occurring in Ukraine were investigated using light and scanning electron microscopy. Palynological data on 6 species are reported for the first time. General pollen morphology of the genus Pedicularis is presented. Three types of apertures (2-syncolpate, 3-colpate, and 3-syncolpate) and seven subtypes of sculpture (3-syncolpate pilate, 3-syncolpate microscabrate-tubeculate, 2-syncolpate microscabrate, 2-syncolpate microscabrate-tubeculate, 2-syncolpate microscabrate-tubeculate-perforate, 2-syncolpate microfoveolate, and 2-syncolpate microscabrate-perforate) are indentified. The subgenus Pedicularis is heterogeneous in its types of apertures and sculpture of the surface, which indicates the need of further taxonomic revision of the group.

scholarly journals Pollen morphology of the Balkan-Carpathian endemic Campanula lingulata Waldst. & Kit. (Campanulaceae)

2016 ◽  
pp. 75-82
Author(s):  
Marina Macukanovic-Jocic ◽  
Snezana Jaric
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Endemic Species ◽  
Pollen Morphology ◽  
Average Length ◽  
Pollen Grains ◽  
Polar Axis ◽  
First Time ◽  
Scanning Electron

Palynomorphological characteristics of Campanula lingulata, the Balkan-Carpathian endemic species growing in Serbia, have been investigated using light microscopy and scanning electron microscopy for the first time, in order to provide some information helpful for a better understanding of the taxonomic position of this species within the genus, as well as to contribute to the pollen atlas of Serbian apiflora. The pollen grains are radially symmetrical, isopolar, 3-zonoporate and medium-sized monads oblate-sphaeroidal in shape. Mean of the polar axis (P) is 27.6?1.9 ?m, while the average length of the equatorial axis (E) is 28.8?1.6 ?m. The apertures are operculate. The sculpturing pattern of the exine is microre?ticulate-microechinatae. The exine surface is covered with evenly distributed supratectal spinules of variable length and sparse granules. The longest supratectal spinules are 0.64?0.05 ?m in length and the smallest sculptural elements are less than 0.2 ?m high. The microechinae density per sample area of 5 ?m x 5 ?m averages 17.4?2.4.

scholarly journals ELECTRON MICROSCOPIC STUDIES OF SOME ANTICANCER PLANTS POLLEN GRAINS

2021 ◽  
Vol 9 (10) ◽  
pp. 1386-1393
Author(s):  
Jayshree Sandesh Thaware ◽  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
Morphological Characters ◽  
Electron Microscopic ◽  
Plant Parts ◽  
Morphological Studies ◽  
Datura Metel ◽  
Scanning Electron

Pollen is appropriately referred by some as Golden dust extremely valuable on account of their tremendous applications in science, industries and public health. No other plant part even though extremely tiny in size is packed with so much information and power. Similar to other plant parts, pollen characters are so varied that the classification system of plants can be built up entirely on the basis of pollen morphology.Palynology is the distinct branch of biology that deals with the dispersed microscopic tiny living and fossil entities including pollen grains, spores, algal and fungal fragments and others. An important aspect of Palynology is the Pollen morphology. The importance of Palynology in taxonomic and phylogenetic consideration of plants is well known. The changes occurring through hybridization and years of cultivation are reflected in pollen morphology. The scope and interest in the study of pollen morphology have widened with the advent of Scanning Electron Microscopy (SEM) and with regards to unipalynous taxa particularly the understanding of finer morphology is of fundamental importance. SEM gives a correct understanding of exine surface as the electron photographs of the surface replica of the exine provides the exact picture of the ornamentation pattern. The variation in the pollen morphological characters helps in the classification of plant taxa and their assessment of their phylogenetic relationship. In the present investigation, the pollen morphological studies were carried out of some ethnomedicinal plants like Catharanthus roseus, Allamanda cathartica, Datura metel, Brassica juncea, Raphanus sativus and Cleome viscosa pollen grains by Scanning electron microscopy. All that they possess anticancer characteristics in common.

Pollen morphology of Sabinaria magnifica (Cryosophileae, Coryphoideae, Arecaceae)

Phytotaxa ◽  
2015 ◽  
Vol 207 (1) ◽  
pp. 135 ◽  
Author(s):  
Giovanni Raul Bogota ◽  
Carina Hoorn ◽  
Wim Star ◽  
Rob Langelaan ◽  
Hannah Banks ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Light Microscopy ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
The Other ◽  
Transmission Electron ◽  
Palm Species ◽  
Scanning Electron

Sabinaria magnifica is so far the only known species in the recently discovered tropical palm genus Sabinaria (Arecaceae). Here we present a complete description of the pollen morphology of this palm species based on light microscopy (LM), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). We also made SEM-based comparisons of Sabinaria with other genera within the tribe Cryosophileae. Pollen grains of Sabinaria magnifica resemble the other genera in the heteropolar, slightly asymmetric monads, and the monosulcate and tectate exine with perforate surface. Nevertheless, there are some clear differences with Thrinax, Chelyocarpus and Cryosophila in terms of aperture and exine. S. magnifica differs from its closest relative, Itaya amicorum, in the exine structure. This study shows that a combination of microscope techniques is essential for the identification of different genera within the Cryosophileae and may also be a necessary when working with other palynologically less distinct palm genera. 

Contribution to the pollen morphology of Tragopogon (Asteraceae) in Turkey

Phytotaxa ◽  
2018 ◽  
Vol 361 (2) ◽  
pp. 168 ◽  
Author(s):  
MUTLU GÜLTEPE ◽  
SERDAR MAKBUL ◽  
SEDA OKUR ◽  
KAMİL COŞKUNÇELEBİ
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
Principal Component ◽  
Numerical Analyses ◽  
Oblate Spheroidal ◽  
Pore Length ◽  
Principal Component Analyses ◽  
Scanning Electron

The pollen morphology of 25 Tragopogon L. taxa (including four subspecies and four varieties) distributed in Turkey was studied under light and scanning electron microscopy. It was observed that pollen grains of the examined Tragopogon taxa are suboblate and oblate-spheroidal in shape, and 3-zonocolpororate with fifteen lacunae. The lenghts of the polar axes and equatorial axes range from 31.57 to 40.35 µm and 35.32 to 44.65 µm, respectively. Numerical analyses show that the length of equatorial axis and the pore length are the most valuable characters among nineteen palynological traits for separating the examined taxa. The general palynological characteristics of the examined taxa allow some taxonomical evaluations for the genus. However, the results of cluster and principal component analyses did not support the division of the genus at any subgeneric level; neither did the ligule colour, as reported in the literature.

Pollen morphology of Ginkgo biloba and Cycas revoluta

1986 ◽  
Vol 64 (12) ◽  
pp. 3075-3078 ◽  
Author(s):  
N. Sahashi ◽  
J. Ueno
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Ginkgo Biloba ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
Spherical Form ◽  
Thin Sections ◽  
Morphological Studies ◽  
Cycas Revoluta ◽  
Scanning Electron

Morphological studies on pollen grains of Ginkgo biloba L. and Cycas revoluta Thunb. were carried out by scanning electron microscopy. The pollen grains of both species are generally oblong with 1-sulcate apertures which are shrunken as a result of dryness. However, the swollen grains show an almost spherical form with a large and rounded germinal aperture. This aperture may not correspond to any aperture type so far known, although the term "anaporate" can be fitted to the swollen pollen grains. Auricular projections, which may be derived from protrusions of the ectosexine, can be seen sometimes on the surface of the pollen grains. These projections remind us of degraded versions of the bladders that may have been present on the pollen grains of the fossil ancestor. The inner side of the exine, which can be seen in thin sections obtained with the freezing microtome, is ornamented with reticulumlike sculptures. These endosculptures may be the first reported among gymnosperm pollen grains.

scholarly journals Distinguishing Oblacinska sour cherry clones (Prunus cerasus L.) by pollen morphology

Genetika ◽  
2020 ◽  
Vol 52 (1) ◽  
pp. 187-198 ◽  
Author(s):  
Dragan Nikolic ◽  
Dragan Milatovic ◽  
Aleksandar Radovic ◽  
Jugoslav Trajkovic
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
Sour Cherry ◽  
Prunus Cerasus ◽  
Morphological Parameters ◽  
Exine Ornamentation ◽  
Pollen Shape ◽  
Scanning Electron

Morphology and ultrastructure of pollen grains were studied in 13 Obla?inska sour cherry clones in three years using scanning electron microscopy (SEM). All studied clones had isopolar, radially symmetric and tricolpate pollen grains. Length and width of pollen grains varied in a range of 50.03-55.97 ?m and 25.16-28.37 ?m respectively. Pollen shape in all the studied clones was identified as prolate. The smallest colpus length was found in the clone 8 (43.73 ?m), and the highest in the clone 3 (49.16 ?m). The highest colpus and mesocolpium width had the clone 3 (1.73 ?m; 14.87 ?m) and the lowest had the clone 7 (1.46 ?m; 13.78 ?m). All studied clones had striate exine ornamentation. Number of ridges per 100 ?m2 of the exine surface was the highest in the clone 11 (16.7) and lowest in the clone 2 (14.1). Ridge and furrow width ranged from 0.46 to 0.61 ?m and from 0.45 to 0.59 ?m, respectively. The clones have been classified into three clusters based on all studied properties. Pollen grains examination by SEM indicated that several morphological parameters (pollen size and exine characteristics) can be used to distinguish Obla?inska sour cherry clones.

scholarly journals Taxonomic significance of pollen morphology in Succisa and Succisella

2019 ◽  
Vol 55 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Zoya M. Tsymbalyuk ◽  
Sergei L. Mosyakin ◽  
Lyudmila M. Nitsenko
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Pollen Morphology ◽  
Pollen Grains ◽  
Polar View ◽  
Taxonomic Significance ◽  
Oblate Spheroidal ◽  
Phylogenetic Studies ◽  
Molecular Phylogenetic ◽  
Scanning Electron

Abstract Pollen morphology of representatives of the genera Succisa and Succisella in the flora of Ukraine was studied using light and scanning electron microscopy. Pollen grains in both taxa were tricolpate, prolate, rarely oblate-spheroidal or spheroidal; large-sized. Their outline in polar view was subcircular, rarely trilobate, in equatorial view elliptical or rarely circular. Colpi short, of variable width, margins irregular with distinct, narrow margo, and blunt or acute ends. Exine sculpture was echinate-microechinate. The revealed characteristics of pollen grains are taxonomically significant at the generic and specific levels, and they can be used in pollen analysis. Palynomorphological data are consistent with the results of recent molecular phylogenetic studies. Data on pollen morphology also confirm taxonomic circumscription of Succiseae V. Mayer & Ehrend. Pollen grains in Succiseae are characterized by a tricolpate aperture type, which was probably ancestral in Dipsacaceae s. str.

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