scholarly journals Laboratory testing of hemolytic properties of materials that come in contact with blood: Comparative application testing method’s two variants according to the standard ASTM F756 in accordance with ISO 10993-4

2010 ◽  
Vol 64 (2) ◽  
pp. 149-156
Author(s):  
Katarina Pavlovic ◽  
Vojislav Bozanic ◽  
Jasna Stanojevic ◽  
Vesna Milicevic ◽  
Bojan Ilic
Keyword(s):  
Incubation Time ◽  
Direct Contact ◽  
Raw Materials ◽  
Osmotic Shock ◽  
Negative Control ◽  
The Other ◽  
Raw Material ◽  
Comparative Approach ◽  
Laboratory Equipment

The presence of hemolytic material in contact with blood may produce increased levels of blood cell lysis and increased levels of plasma hemoglobin. This may induce toxic effects or other effects which may stress the kidneys or other organs. In this paper two variants of in vitro method and obtained results? comparison were presented for testing of hemolytic properties of six raw materials (Polipropylene Moplen EP 540 P, Policarbonate colorless 164 R-112, Policarbonate brown 164 R-51918, Polietylene NG 3026 K, Polietylene NG - Purell GB 7250, Polietylene VG - Hiplex 5502) for medical device manufacturing and one raw material (Polietylen NG granulate) used for infusion solutions?s plastic bottles manufacturing. One of method?s variants relies on raw material direct contact with swine blood and the other on extract of the material contact with swine blood. Both method?s variants imply reading of the absorbance of the supernatant after tubes were incubated and centrifuged. According to values obtained and using the standard curve free hemoglobin concentration is determined and based on this percentage hemolysis of raw material. Positive and negative controls were used in both variants where water for injection (WFI) was used as positive control in which partial or complete hemolysis of erythrocytes occurs due to osmotic shock and phosphate buffer saline was used as negative control with no hemolytic property. In this paper comparison of results obtained by both method?s variants for testing of seven raw materials was presented, while these conclusions can not be used neither for all materials, nor for all applications without preliminary testing using both variants and then choosing more sensitive and more reliable one. It was shown and stated in the paper as well that incubation time being 3, 15 or 24 h, had no impact on the variant?s with direct contact sensitivity. This comparative approach was used for drawing conclusions in terms of suitability for application of one or the other method?s variant, as well as for defining relevant incubation time and finally for choosing more sensitive and more reliable variant for assessment of hemolytic properties of raw materials. Variant with direct contact was chosen from the aspect of less complexity regarding necessary laboratory equipment which makes it economically more favorable and fit for the purpose.

scholarly journals Investigation of potential anti-urolithiatic activity from different types of Musa pseudo-stem extracts in inhibition of calcium oxalate crystallization

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Mazni Abu Zarin ◽  
Joo Shun Tan ◽  
Paramasivam Murugan ◽  
Rosma Ahmad
Keyword(s):  
Calcium Oxalate ◽  
Incubation Time ◽  
Positive Control ◽  
Negative Control ◽  
Aggregation Assay ◽  
Banana Tree ◽  
Methanolic Extracts ◽  
Different Types ◽  
Aggregation Activity

Abstract Background The banana or scientifically referred to as Musa sp., is one of the most popular fruits all over the world. Almost all parts of a banana tree, including the fruits, stem juice, and flowers are commonly used as traditional medicine for treating diarrhoea (unripe), menorrhagia, diabetes, dysentery, and antiulcerogenic, hypoglycemic, antilithic, hypolipidemic conditions, plus antioxidant actions, inflammation, pains and even snakebites. The study carried out was to evaluate in vitro anti-urolithiatic activity from different types of Musa pseudo-stems. Methods Observing anti-urolithiathic activity via in vitro nucleation and aggregation assay using a spectrophotometer followed by microscopic observation. A total of 12 methanolic extracts were tested to determine the potential extracts in anti-urolithiasis activities. Cystone was used as a positive control. Results The results manifested an inhibition of nucleation activity (0.11 ± 2.32% to 55.39 ± 1.01%) and an aggregation activity (4.34 ± 0.68% to 58.78 ± 1.81%) at 360 min of incubation time. The highest inhibition percentage in nucleation assay was obtained by the Musa acuminate x balbiciana Colla cv “Awak Legor” methanolic pseudo-stem extract (2D) which was 55.39 ± 1.01%at 60 min of incubation time compared to the cystone at 30.87 ± 0.74%. On the other hand,the Musa acuminate x balbiciana Colla cv “Awak Legor” methanolic bagasse extract (3D) had the highest inhibition percentage in the aggregation assay incubated at 360 min which was obtained at 58.78 ± 1.8%; 5.53% higher than the cystone (53.25%).The microscopic image showed a great reduction in the calcium oxalate (CaOx) crystals formation and the size of crystals in 2D and 3D extracts, respectively, as compared to negative control. Conclusions The results obtained from this study suggest that the extracts are potential sources of alternative medicine for kidney stones disease.

Bioaccessibility of Anthocyanins on in vitro Digestion Mmodels: Factors Implicated and Role in Functional Foods Development

2021 ◽  
Vol 28 ◽  
Author(s):  
Gabriel Prado ◽  
Isidora Pierattini ◽  
Guiselle Villarroel ◽  
Fernanda Fuentes ◽  
Alejandra Silva ◽  
...  
Keyword(s):  
Functional Foods ◽  
Raw Materials ◽  
Food Product ◽  
In Vitro Digestion ◽  
Raw Material ◽  
Simulated Digestion ◽  
Prevalence Of Obesity ◽  
Food Matrices

Background: Worldwide, the prevalence of obesity and related non-communicable chronic diseases is high and continues to grow. In that sense, anthocyanins (ANC) have shown beneficial health effects in preventing obesity and metabolic risk factors. Moreover, the demand for functional foods incorporating these compounds has risen significantly in the past years. Thus, there is a need for validations of the functional properties of these formulations; nevertheless, in vivo assays are complex and require a lot of resources. One approach for estimating bioactive compounds' functionality and health benefits is to evaluate their bioaccessibility on a specific food matrix, determined by various factors. This article aims to review different factors influencing the bioaccessibility of ANC evaluated on in vitro digestion models as a functionality parameter, elucidating the effect of chemical composition, raw materials, food matrices, and vehicles for the delivery of ANC. Methods: Study searches were performed using PubMed, Web of Science, Scopus, and Science Direct databases. Results: Different factors influenced bioaccessibility and stability of ANC studied by in vitro digestion which are: i) the raw material used for ANC obtention; ii) food processing; iii) other food components; iv) the extraction method and solvents used; v) the structure of ANC; vi) delivery system (e.g., microencapsulation); vii) pH of the medium; viii) the digestion stage. Conclusion: Simulated digestion systems allow to determine free or encapsulated ANC bioaccessibility in different food matrices, which offers advantages in determining the potential functionality of a food product.

HEMATOPOETIC STEM CELL RESPONSE TO STIMULATION OF EXTRACTS FROM ANIMAL MATERIALS

2016 ◽  
Vol 78 (5-6) ◽  
Author(s):  
Ivan Smirnov ◽  
Victor Keino ◽  
Ksenia Goryacheva ◽  
Alexander Shunk ◽  
Alexander Bondarev ◽  
...  
Keyword(s):  
Raw Materials ◽  
Raw Material ◽  
Animal Origin ◽  
Aqueous Extracts ◽  
Second Stage ◽  
Hematopoetic Stem Cell ◽  
Two Stages ◽  
Stimulation Of

The article presents the results of the research hemostimulating activity of aqueous extracts of antler young Siberean stag and drone larvae homogenate. These substrates were obtained from raw materials of animal origin. Altai Krai andAltaiRepublicare subjects of theRussian Federationwhich is the place of production of the raw material. Experiments were conducted in two stages. The first stage - in vitro, which included a research of experimental substrates on the culture of mouse marrow cells. During the experiments were obtained different results. We counted the number of colonies grown in cell culture for this. The second stage of experimenters - in vivo. It included an assessment of the myeloprotector on model of cytostatic myelosuppression of mice and analysis of bone marrow and peripheral blood.

Effect of Different Intermediary Bases on Microleakage of a Restorative Material in Class II Box Cavities of Primary Teeth

2017 ◽  
Vol 40 (2) ◽  
pp. 82-87 ◽  
Author(s):  
Faika Y. Abdelmegid ◽  
Fouad S. Salama ◽  
Waleed M. Al-Mutairi ◽  
Saud K. Al-Mutairi ◽  
Sultan O. Baghazal
Keyword(s):  
Primary Teeth ◽  
In Vitro Study ◽  
Class Ii ◽  
Positive Control ◽  
Negative Control ◽  
The Other ◽  
Control Groups ◽  
Significant Difference ◽  
The Mean

Introduction The aim of this in vitro study was to assess and compare the effect of different intermediary bases on microleakage between tooth and a nanocomposite interface in Class II box cavities in primary teeth. Methods Standard Class II box cavities were prepared in 52 primary molars and randomly divided into 9 groups according to the intermediary base used (Multicore Flow, Fuji II LC, SDR, Smart Dentin Replacement, and Biodentine). All specimens were subjected to thermocycling and prepared for microleakage testing and evaluation. Results There was significant difference in the mean ranks of microleakage between the 9 groups, which was observed in the gingival side (p<0.0001) and the occlusal side (p<0.0001). The mean ranks microleakage was significantly higher with experimental SDR, experimental Multicore Flow, and positive control materials when compared with the other 6 groups. The microleakage mean ranks were statistically significantly lower in experimental Fuji II LC, experimental Biodentine, and all negative control groups when compared with the other 3 groups. Conclusions Microleakage is affected by the application of intermediate material. Experimental Biodentine and Fuji II LC showed the lowest microleakage while experimental SDR and experimental Multicore Flow showed the highest microleakage.

scholarly journals Traditional and new raw materials for spirit beverage production

2021 ◽  
Vol 17 ◽  
pp. 70-78
Author(s):  
Szymon Ługowoj ◽  
Maria Balcerek
Keyword(s):  
Ethanol Production ◽  
Raw Materials ◽  
The Other ◽  
Raw Material ◽  
Fast Growing ◽  
Growth Requirements ◽  
Other Hand ◽  
Materials Used ◽  
Production Industry

The ethanol production industry is a fast growing branch of the economy in many countries, and there is a rich tradition of spirit beverage production of many unique drinks such as Polish vodka and Starka or Irish and Scotch whisk(e)y, all of which have unique organoleptic features. This variety is possible thanks to different raw materials used for production such as rye, barley or corn and potatoes, as well as technological solutions developed over the generations of manufacturing. Rye deserves a closer look due to its low growth requirements and many different uses as well as its long tradition of cultivation, especially in Poland. On the other hand, manufacturers are currently interested in using new, original raw materials for the production of so-called craft alcohols. Buckwheat is an example of a raw material that can be successfully used in the production of original spirits.

scholarly journals Effects of Melia azedarach L. leaf and fruit on mineralization of carbon in soil

2014 ◽  
Vol 43 (1) ◽  
pp. 119-122
Author(s):  
Nacide Kizildag ◽  
Sahen Cenkseven ◽  
Husniye Aka Sagliker ◽  
Cengiz Darici
Keyword(s):  
Incubation Time ◽  
Carbon Mineralization ◽  
The Other ◽  
Melia Azedarach ◽  
In Vitro Incubation

Carbon mineralization in soil increased significantly due to additions of pure azadirachtin and powdered leaves and fruits of Melia azedarach L. under in vitro incubation for 30 days at 28°C. Cumulative respired C(CO2) clearly increased with incubation time in all treatments except in soil mixed with pure azadirachtin (p < 0.001). Carbon mineralization ratio in soils mixed with single doses of powdered leaf and fruit were significantly higher than the other doses tested.  

scholarly journals Aktivitas Antimalaria Daun Gempol (Nauclea orientalis (L.) L) terhadap Plasmodium falciparum

2020 ◽  
Vol 30 (2) ◽  
pp. 135-146
Author(s):  
Mery Budiarti ◽  
Anshary Maruzy ◽  
Nengah Ratri RK ◽  
Endang Brotojoyo
Keyword(s):  
Plasmodium Falciparum ◽  
Antimalarial Activity ◽  
Raw Materials ◽  
Raw Material ◽  
Plant Parts ◽  
Phytochemical Compounds ◽  
Bioactive Potential ◽  
Layer Chromatography ◽  
Selection Of

Abstract The use of gempol (Nauclea orientalis (L.) L) stem as a malaria medicine has been empirically and scientifically proven. This condition encourages exploitation which can lead to scarcity of raw materials. Substitution of plant parts as raw material for medicine is one of the innovations that can be done for the sustainability of the plant species. Leaves are one part of the plant that is often used as a raw material for medicine. The selection of leaves as the main ingredient has many advantages over other parts. Until now, research related to the antimalarial bioactive potential of N. orientalis (L.) L leaves has not been published. Therefore, this study aimed to explore the potential for leaf antimalarial activity as an alternative to substitution of stem parts. The leaf extract of N. orientalis (L.) L was prepared by maceration method with 96% ethanol then fractionation was carried out in stage using hexane, ethyl acetate and methanol as solvents. Antimalarial activity testing was carried out in vitro against Plasmodium falciparum 3D7 and Thin Layer Chromatography (TLC) for screening phytochemical compounds in each sample. The hexane solvent was known to produce the most optimum extract by with a yield of 20%. The antimalarial activity of the hexane (IC 50 1.93 μg/mL) and methanol (IC 3.91 μg/ mL) fractions were classified as ‘very active’ and had a tendency to be able to compete with chloroquine phosphate activity. The potential for antimalarial activity in both samples was influenced by the content of alkaloids, steroids, flavonoids and terpenoids which had been developed as active ingredients for malaria drugs. The results of this study indicate that the leaves of Nauclea orientalis (L.) L have the potential to be developed as an alternative to malaria medicine. Abstrak Pemanfaatan batang gempol (Nauclea orientalis (L.) L) sebagai obat malaria telah terbukti secara empiris dan ilmiah. Kondisi tersebut mendorong terjadinya eksploitasi hingga dapat berujung pada kelangkaan bahan baku. Substitusi bagian tumbuhan sebagai bahan baku obat merupakan salah satu inovasi yang dapat dilakukan untuk keberlanjutan hidup spesies tumbuhan tersebut. Daun merupakan salah satu bagian tumbuhan yang sering digunakan sebagai bahan baku obat. Pemilihan daun sebagai bahan utama memiliki banyak kelebihan dibandingkan bagian lainnya. Penelitian terkait potensi bioaktif antimalaria daun Nauclea orientalis (L.) L hingga saat ini belum dipublikasikan. Oleh karena itu, penelitian ini bertujuan untuk menggali potensi aktivitas antimalaria daun sebagai alternatif subtitusi bagian batang. Ekstrak daun Nauclea orientalis (L.) L disiapkan dengan metode maserasi dengan etanol 96%, kemudian dilakukan fraksinasi cair-cair bertingkat menggunakan pelarut heksana, etil asetat, dan metanol. Pengujian aktivitas antimalaria dilakukan secara in vitro terhadap Plasmodium falciparum 3D7 dan Kromatografi Lapis Tipis (KLT) untuk penapisan senyawa fitokimia pada masingmasing sampel. Pelarut heksana diketahui menghasilkan ekstrak paling optimum dengan rendemen 20%. Aktivitas antimalaria fraksi heksana (IC 50 1,93 µg/mL) dan metanol (IC 3,91 µg/mL) yang tergolong dalam kategori ‘sangat aktif, serta memiliki kecenderungan mampu bersaing dengan aktivitas klorokuin fosfat. Potensi aktivitas antimalaria pada kedua sampel tersebut dipengaruhi oleh kandungan senyawa alkaloid, steroid, flavonoid dan terpenoid yang telah banyak dikembangkan sebagai bahan aktif obat malaria. Hasil penelitian ini menunjukkan bahwa daun Nauclea orientalis (L.) L berpotensi untuk dikembangkan sebagai alternatif obat malaria.

scholarly journals Assessing the possibility of using dry concentration for oxidized pegmatite and granites at the Kedrovoe feldspar deposit

2021 ◽  
Vol 1 (7) ◽  
pp. 45-54
Author(s):  
Tatiana A. Buzunova ◽  
◽  
Varvara N. Shigaeva ◽  
Keyword(s):  
Raw Materials ◽  
Raw Material ◽  
Processing Plant ◽  
Resource Saving ◽  
Laboratory Equipment ◽  
Flotation Reagents ◽  
Feldspar Concentrate ◽  
Silica Alumina ◽  
Concentration Methods ◽  
Tailings Ponds

Introduction. Feldspar raw material is a natural source of silica, alumina, and alkali metal oxides. Each type of feldspar is distinguished by its applications and concentration methods. The main effective method of feldspar raw material concentration is flotation in the course of which the majority of harmful impurities are separated. However, this method is rather costly due to flotation reagents purchase, tailings ponds organization and maintenance, etc. Research relevance. Feldspar dry concertation technologies are promising in terms of resource-saving but uncommon. So, the development of a dry concertation technology for feldspar raw materials seems highly relevant. Research objective is to study the possibility of employing dry concentration technology to process feldspar raw materials at the new Kedrovoe deposit Methods of research. Laboratory equipment of JSC Uralmekhanobr was used for the research, namely centrifugal crusher DC-0.5; centrifugal deflection mill; laboratory-scale cascade classifier; dry electromagnetic separator SMS-20M ITOMAK, and tribo electrostatic separator. Results. The trials confirmed that it is possible to process Kedrovoe feldspar raw materials by dry methods and effectively use centrifugal crushing and grinding as preparation of raw materials for concentration. Feldspar concentrate with a mass fraction of Fe2O3 – 0.30%; SiO2 – 69.42%; Al2O3 – 17.36%; K2O + Na2O – 11.84% has been obtained, which meets the specifications. Conclusions. The technological studies confirmed the possibility in principle of using feldspar raw material dry concentration for oxidized pegmatite and granites of the Kedrovoe deposit at the processing plant of Malyshevskoe Ore Management JSC. Saleable feldspar concentrate has been obtained.

scholarly journals Exploiting of Secondary Raw Materials from Fish Processing Industry as a Source of Bioactive Peptide-Rich Protein Hydrolysates

Marine Drugs ◽  
2021 ◽  
Vol 19 (9) ◽  
pp. 480
Author(s):  
Girija Gajanan Phadke ◽  
Nikheel Bhojraj Rathod ◽  
Fatih Ozogul ◽  
Krishnamoorthy Elavarasan ◽  
Muthusamy Karthikeyan ◽  
...  
Keyword(s):  
Bioactive Peptides ◽  
Raw Materials ◽  
Scientific Information ◽  
Amino Acid Sequences ◽  
Raw Material ◽  
Fish Processing ◽  
Secondary Raw Materials ◽  
Fish And Shellfish

Developing peptide-based drugs are very promising to address many of the lifestyle mediated diseases which are prevalent in a major portion of the global population. As an alternative to synthetic peptide-based drugs, derived peptides from natural sources have gained a greater attention in the last two decades. Aquatic organisms including plants, fish and shellfish are known as a rich reservoir of parent protein molecules which can offer novel sequences of amino acids in peptides, having unique bio-functional properties upon hydrolyzing with proteases from different sources. However, rather than exploiting fish and shellfish stocks which are already under pressure due to overexploitation, the processing discards, regarded as secondary raw material, could be a potential choice for peptide based therapeutic development strategies. In this connection, we have attempted to review the scientific reports in this area of research that deal with some of the well-established bioactive properties, such as antihypertensive, anti-oxidative, anti-coagulative, antibacterial and anticarcinogenic properties, with reference to the type of enzymes, substrate used, degree of particular bio-functionality, mechanism, and wherever possible, the active amino acid sequences in peptides. Many of the studies have been conducted on hydrolysate (crude mixture of peptides) enriched with low molecular bioactive peptides. In vitro and in vivo experiments on the potency of bioactive peptides to modulate the human physiological functions beneficially have demonstrated that these peptides can be used in the prevention and treatment of non-communicable lifestyle mediated diseases. The information synthesized under this review could serve as a point of reference to drive further research on and development of functionally active therapeutic natural peptides. Availability of such scientific information is expected to open up new zones of investigation for adding value to underutilized secondary raw materials, which in turn paves the way for sustainability in fish processing. However, there are significant challenges ahead in exploring the fish waste as a source of bioactive peptides, as it demands more studies on mechanisms and structure–function relationship understanding as well as clearance from regulatory and statutory bodies before reaching the end user in the form of supplement or therapeutics.

scholarly journals Parameters of obtaining tincture from underground organs of Sanguisorba officinalisand study its biological activity

2021 ◽  
Vol 14 (3) ◽  
pp. 299-305
Author(s):  
T. V. Oproshanska ◽  
O. P. Khvorost
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Activity ◽  
Growth Retardation ◽  
Raw Materials ◽  
Hydroxycinnamic Acids ◽  
Raw Material ◽  
Optimal Parameters ◽  
Antimicrobial And Antioxidant Activity ◽  
Sanguisorba Officinalis

The aim of the work is to experimentally determine the optimal parameters for obtaining a tincture of rhizomes with roots of Sanguisorba officinalis and to investigate its antimicrobial and antioxidant activity. Materials and methods. Rhizomes with roots of Sanguisorba officinalis were harvested in autumn 2019. When establishing the optimal parameters of the technology of obtaining tincture, the evaluation criterion was the number of the sum of polyphenols and the amount of hydroxycinnamic acids, which were determined by spectrophotometric method according to the methods of the State Pharmacopoeia of Ukraine 2.0. Antimicrobial activity was studied by agar diffusion and serial dilutions, antioxidant – in vitro using a stable radical 2,2-diphenyl-1-picrylhydrazyl (2,2-diphenyl-1-picrylhydrazyl – DPPH). Results. The optimal parameters for obtaining a tincture of rhizomes with roots of Sanguisorba officinalis was extraction method maceration at room temperature, raw material-finished product ratio 1:5, time 48 hours, extractant 50 % ethyl alcohol, the number of polyphenols, and the number of hydroxycinnamic acids in terms of dry raw materials not less than 4.0 % and 1.5 % respectively. The resulting tincture exhibits antimicrobial activity against microorganisms Bacillus subtilis ATCC 6633 (growth retardation 26.40 ± 1.04 mm), Staphylococcus aureus ATCC 25923 (growth retardation 24.60 ± 0.68 mm) and Pseudomonas aeruginosa ATCС 27853 (growth retardation 23.60 ± 0.68 mm) and at a dose of 0.02 ml showed antioxidant activity at the level of 70 %. Conclusions. The parameters of obtaining a tincture of rhizomes with roots of Sanguisorba officinalis were determined and its antimicrobial and antioxidant activity was studied.

Sign in / Sign up

Export Citation Format

Share Document