Immune Profile of Infectious Bursal Disease (IBD). II. Effect of IBD Virus on Pokeweed-Mitogen-Stimulated Peripheral Blood Lymphocytes of Chickens

1980 ◽  
Vol 24 (3) ◽  
pp. 734 ◽  
Author(s):  
V. Sivanandan ◽  
S. K. Maheswaran
1980 ◽  
Vol 47 (3) ◽  
pp. 305-311 ◽  
Author(s):  
Carlos Concha ◽  
Olof Holmberg ◽  
Bror Morein

SUMMARYFrom bovine mammary secretion during the dry period, the total number of cells was between 1·2 and 5·9 Ǻ 106/ml. A mean of 35% of these cells were classified as lymphocytes and ∼ 85% of them could be isolated by the Ficoll-isopac method. Centrifugation separated 6% of the cells into the fat; 5% of them were lymphocytes. About 47% of the lymphocytes bound Helix pomatia agglutinin, a T-cells marker, while the proportion of Ig-bearing cells was ∼ 28%. The mammary lymphocytes were stimulated by the lectins phytohaemagglutinin, pokeweed mitogen, concanavalin A and by lipopolysaccharide from Salmonella typhimurium. The stimulation indices of mammary lymphocytes were generally lower than those for peripheral blood lymphocytes from the same animals. The background values, i.e. counts/min of lymphocytes incubated without mitogen, were often higher for lymphocytes isolated from mammary secretion than from blood.


1990 ◽  
Vol 20 (10) ◽  
pp. 2191-2199 ◽  
Author(s):  
Gang Qiu ◽  
Jean-Francois Gauchat ◽  
Monique Vogel ◽  
Michele Mandallaz ◽  
Alain L. de Weck ◽  
...  

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