High Glucose Prolongs Cell-Cycle Traversal of Cultured Human Endothelial Cells

Diabetes ◽  
1987 ◽  
Vol 36 (11) ◽  
pp. 1261-1267 ◽  
Author(s):  
M. Lorenzi ◽  
J. A. Nordberg ◽  
S. Toledo
Keyword(s):  
Cell Cycle ◽  
Endothelial Cells ◽  
High Glucose ◽  
Human Endothelial Cells

High glucose prolongs cell-cycle traversal of cultured human endothelial cells

Diabetes ◽  
1987 ◽  
Vol 36 (11) ◽  
pp. 1261-1267 ◽  
Author(s):  
M. Lorenzi ◽  
J. A. Nordberg ◽  
S. Toledo
Keyword(s):  
Cell Cycle ◽  
Endothelial Cells ◽  
High Glucose ◽  
Human Endothelial Cells

Dual effect of oxidized LDL on cell cycle in human endothelial cells through oxidative stress

2001 ◽  
Vol 59 (s78) ◽  
pp. 120-123 ◽  
Author(s):  
Jan Galle ◽  
Alexandra Heinloth ◽  
Christoph Wanner ◽  
Kathrin Heermeier
Keyword(s):  
Oxidative Stress ◽  
Cell Cycle ◽  
Endothelial Cells ◽  
Oxidized Ldl ◽  
Human Endothelial Cells ◽  
Dual Effect

PS 04-09 HIGH GLUCOSE AND PALMITATE INCREASES BONE MORPHOGENIC PROTEIN 4 EXPRESSION IN HUMAN ENDOTHELIAL CELLS

2016 ◽  
Vol 34 (Supplement 1) ◽  
pp. e136
Author(s):  
Hyuk Sang Kwon ◽  
Oak Kee Hong ◽  
Jang Won Son ◽  
Soon Jib Yoo ◽  
Ki Ho Song ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
High Glucose ◽  
Bone Morphogenic Protein ◽  
Human Endothelial Cells ◽  
Bone Morphogenic Protein 4

scholarly journals Global Transcriptional Profiling Reveals Novel Autocrine Functions of Interleukin 6 in Human Vascular Endothelial Cells

2020 ◽  
Vol 2020 ◽  
pp. 1-12 ◽  
Author(s):  
Liza U. Ljungberg ◽  
Mulugeta M. Zegeye ◽  
Caroline Kardeby ◽  
Knut Fälker ◽  
Dirk Repsilber ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Endothelial Cells ◽  
Pathway Analysis ◽  
Interleukin 6 ◽  
Vascular Endothelial Cells ◽  
Differential Regulation ◽  
Vascular Endothelial ◽  
Human Endothelial Cells ◽  
Cellular Functions ◽  
Cellular Movement

Background. Interleukin 6 (IL6) is a multifunctional cytokine produced by various cells, including vascular endothelial cells. IL6 has both pro- and non-/anti-inflammatory functions, and the response to IL6 is dependent on whether it acts via the membrane-bound IL6 receptor α (IL6Rα) (classic signaling) or the soluble form of the receptor (transsignaling). As human endothelial cells produce IL6 and at the same time express IL6Rα, we hypothesized that IL6 may have autocrine functions. Methods. Knockdown of IL6 in cultured human endothelial cells was performed using siRNA. Knockdown efficiency was evaluated using ELISA. RNA sequencing was employed to characterize the transcriptional consequence of IL6 knockdown, and Ingenuity Pathway Analysis was used to further explore the functional roles of IL6. Results. Knockdown of IL6 in cultured endothelial cells resulted in a 84-92% reduction in the release of IL6. Knockdown of IL6 resulted in dramatic changes in transcriptional pattern; knockdown of IL6 in the absence of soluble IL6Rα (sIL6Rα) led to differential regulation of 1915 genes, and knockdown of IL6 in the presence of sIL6Rα led to differential regulation of 1967 genes (fold change 1.5, false discovery rate<0.05). Pathway analysis revealed that the autocrine functions of IL6 in human endothelial cells are mainly related to basal cellular functions such as regulation of cell cycle, signaling, and cellular movement. Furthermore, we found that knockdown of IL6 activates functions related to adhesion, binding, and interaction of endothelial cells, which seem to be mediated mainly via STAT3. Conclusion. In this study, a large number of novel genes that are under autocrine regulation by IL6 in human endothelial cells were identified. Overall, our data indicate that IL6 acts in an autocrine manner to regulate basal cellular functions, such as cell cycle regulation, signaling, and cellular movement, and suggests that the autocrine functions of IL6 in human endothelial cells are mediated via IL6 classic signaling.

scholarly journals High Glucose Causes Upregulation of Cyclooxygenase-2 and Alters Prostanoid Profile in Human Endothelial Cells

Circulation ◽  
2003 ◽  
Vol 107 (7) ◽  
pp. 1017-1023 ◽  
Author(s):  
Francesco Cosentino ◽  
Masato Eto ◽  
Paola De Paolis ◽  
Bernd van der Loo ◽  
Markus Bachschmid ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
High Glucose ◽  
Cyclooxygenase 2 ◽  
Human Endothelial Cells

Modification of Tissue-Factor mRNA and Protein Response to Thrombin and Interleukin 1 by High Glucose in Cultured Human Endothelial Cells

Diabetes ◽  
1989 ◽  
Vol 38 (2) ◽  
pp. 212-218 ◽  
Author(s):  
D. Boeri ◽  
F. E. Almus ◽  
M. Maiello ◽  
E. Cagliero ◽  
L. V. M. Rao ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Tissue Factor ◽  
High Glucose ◽  
Interleukin 1 ◽  
Human Endothelial Cells ◽  
Protein Response
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