scholarly journals SELEKSI GALUR DARI POPULASI F4 KEDELAI YANG TAHAN TERHADAP PENYAKIT MOSAIK (Soybean mosaic virus) DAN BERDAYA HASIL TINGGI

2014 ◽  
Vol 14 (2) ◽  
pp. 152-159
Author(s):  
Wuye Ria Andayanie ◽  
Praptiningsih Gamawati Adinurani
Keyword(s):  
Mosaic Virus ◽  
Seed Size ◽  
Crop Yields ◽  
Soybean Mosaic Virus ◽  
Mosaic Disease ◽  
High Yield ◽  
New Variety ◽  
Moderately Resistant ◽  
Screen House ◽  
Selection Of

Soybean lines selection of F4 population resistant  to soybean mosaic disease (Soybean mosaic virus) with high yield.  The soybean breeding program is usually not purposedly done for resistance to Soybean mosaic virus (SMV) but rather for crop yields. The experiment was aimed to obtain soybean lines of F4 population resistant to soybean mosaic disease with high yield.  F2-F4 plants that have been inoculated with the T isolate of SMV one week after planting were selected by the pedigree  in the screen house. The result indicated eight  F4 populations (Wilis x L. Temanggung; Wilis x L. Jombang; Wilis x Pangrango; Wilis x PI 200485;  Gepak Kuning x L. Jombang; Gepak Kuning x L. Temanggung; Gepak Kuning x Malabar; Gepak Kuning x PI 200485) produced medium seed size (from 9.84-10.26 g 100/seeds).  Gepak Kuning x Mlg 3288  showed more resistant than Gepak Kuning x PI 200485. The seed produced by Gepak Kuning x PI 200485 was 1.97 ton/ha. There were no F4 populations that had higher yield and bigger seed size than Gepak Kuning x PI 200485 even though they were  moderately resistant to SMV. Therefore, these lines of Gepak Kuning x Mlg 3288 and Gepak  Kuning x  PI 200485 might provide exellent sources to develop a new variety that resistant to SMV and of high yield.

Evaluation of germ plasm for resistant to Soybean mosaic virus (SMV)

2020 ◽  
Vol 21 (1) ◽  
pp. 6-9
Author(s):  
Wuye Ria Andayanie
Keyword(s):  
Abiotic Stress ◽  
Environmental Factors ◽  
Mosaic Virus ◽  
Polyclonal Antibody ◽  
Symptom Severity ◽  
Germ Plasm ◽  
Polyclonal Antibodies ◽  
Soybean Mosaic Virus ◽  
High Yield ◽  
High Yields

Soybean superior varieties with high yields and are resistant to abiotic stress have been largely released, although some varieties grown in the field are not resistant to SMV. In addition, the opportunity to obtain lines of hope as prospective varieties with high yield and resistance to SMV is very small. The method for evaluating soybean germplasm is based on serological observations of 98 accessions of leaf samples from SMV inoculation with T isolate. The evaluation results of 98 accessions based on visual observations showed 31 genotypes reacting very resistant or healthy to mild resistant category to SMV T isolate  with a percentage of symptom severity of 0 −30 %. Among 31 genotypes there are 2 genotypes (PI 200485; M8Grb 44; Mlg 3288) with the category of visually very resistant and resistant, respectively and  Mlg 3288  with the category of mild resistant.  They have a good agronomic appearance with a weight of 100 seeds (˃10 g) and react negatively with polyclonal antibodies to SMV, except Mlg 3288 reaction is not consistent, despite the weight of 100 seeds (˃ 10 g). Leaf samples from 98 accessions revealed various symptoms of SMV infection in the field. This diversity of symptoms is caused by susceptibility to accession, when infection occurs, and environmental factors. Keywords—: soybean; genotipe; Soybean mosaic virus (SMV); disease severity; polyclonal  antibody

scholarly journals Milestones in research on tobacco mosaic virus

1999 ◽  
Vol 354 (1383) ◽  
pp. 521-529 ◽  
Author(s):  
B. D. Harrison ◽  
T. M. A. Wilson
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Virus Resistance ◽  
Dominant Role ◽  
Biological Properties ◽  
Mosaic Disease ◽  
Genetically Engineered ◽  
High Yield ◽  
Past Century ◽  
Avirulence Genes

Beijerinck's (1898) recognition that the cause of tobacco mosaic disease was a novel kind of pathogen became the breakthrough which led eventually to the establishment of virology as a science. Research on this agent, tobacco mosaic virus (TMV), has continued to be at the forefront of virology for the past century. After an initial phase, in which numerous biological properties of TMV were discovered, its particles were the first shown to consist of RNA and protein, and X–ray diffraction analysis of their structure was the first of a helical nucleoprotein. In the molecular biological phase of research, TMV RNA was the first plant virus genome to be sequenced completely, its genes were found to be expressed by cotranslational particle disassembly and the use of subgenomic mRNA, and the mechanism of assembly of progeny particles from their separate parts was discovered. Molecular genetical and cell biological techniques were then used to clarify the roles and modes of action of the TMV non–structural proteins: the 126 kDa and 183 kDa replicase components and the 30 kDa cell–to–cell movement protein. Three different TMV genes were found to act as avirulence genes, eliciting hypersensitive responses controlled by specific, but different, plant genes. One of these (the N gene) was the first plant gene controlling virus resistance to be isolated and sequenced. In the biotechnological sphere, TMV has found several applications: as the first source of transgene sequences conferring virus resistance, in vaccines consisting of TMV particles genetically engineered to carry foreign epitopes, and in systems for expressing foreign genes. TMV owes much of its popularity as a research model to the great stability and high yield of its particles. Although modern methods have much decreased the need for such properties, and TMV may have a less dominant role in the future, it continues to occupy a prominent position in both fundamental and applied research.

Interactions between soybean, Bradyrhizobium japonicum and Soybean mosaic virus: the effects depend on the interaction sequence

2019 ◽  
Vol 46 (11) ◽  
pp. 1036 ◽  
Author(s):  
Sofía Andreola ◽  
Marianela Rodriguez ◽  
Rodrigo Parola ◽  
Sergio Alemano ◽  
Ramiro Lascano
Keyword(s):  
Salicylic Acid ◽  
Virus Infection ◽  
Mosaic Virus ◽  
Bradyrhizobium Japonicum ◽  
Crop Yields ◽  
Soybean Mosaic Virus ◽  
Single Infection ◽  
Sugar Accumulation ◽  
Primary Metabolism ◽  
Tripartite Interaction

The symbiotic interaction between soybean and nitrogen-fixing rhizobia can lead to plant growth promotion and induced systemic responses. Symbiotic interactions may increase tolerance/resistance to abiotic/biotic stress conditions, but are also sensitive to environmental conditions. Soybean mosaic virus (SMV), which is transmitted by seed and aphids, severely affects crop yields in many areas of the world, consequently virus infection may precede rhizobium infection or vice versa in the field. With the hypothesis that sequence of interaction is a key determinant of the resulting responses; growth, primary metabolism and defence responses were evaluated in different interaction sequences. Results showed that vegetative growth was promoted by Bradyrhizobium japonicum (Bj) inoculation and drastically impaired by SMV infection. The negative effect of SMV single infection on soybean growth parameters was correlated with photosynthesis decrease, sugar accumulation, oxidative damage, and increases in salicylic acid levels. Bj inoculation partially reversed virus-induced symptoms, mainly at Bj-SMV sequence. However, this symptom attenuation did not correlate with less virus accumulation. Nodulation was negatively affected by SMV, particularly when virus infection was previous to Bj inoculation (SMV-Bj). Defence related hormones (salicylic acid (SA)/jasmonic acid (JA)) and the expression of defence-related genes were dependent on the sequence of tripartite interaction. The present study showed that the sequence of the tripartite interaction among soybean, Bj and SMV determinates the tolerance/susceptibility to SMV infection, through changes in the defence mechanism and metabolic alteration.

scholarly journals Novel roles for well-known players: from tobacco mosaic virus pests to enzymatically active assemblies

2016 ◽  
Vol 7 ◽  
pp. 613-629 ◽  
Author(s):  
Claudia Koch ◽  
Fabian J Eber ◽  
Carlos Azucena ◽  
Alexander Förste ◽  
Stefan Walheim ◽  
...  
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Self Assembly ◽  
Building Blocks ◽  
Mosaic Disease ◽  
Inorganic Materials ◽  
High Yield ◽  
External Control ◽  
Immobilization Of Enzymes

The rod-shaped nanoparticles of the widespread plant pathogentobacco mosaic virus(TMV) have been a matter of intense debates and cutting-edge research for more than a hundred years. During the late 19th century, their behavior in filtration tests applied to the agent causing the 'plant mosaic disease' eventually led to the discrimination of viruses from bacteria. Thereafter, they promoted the development of biophysical cornerstone techniques such as electron microscopy and ultracentrifugation. Since the 1950s, the robust, helically arranged nucleoprotein complexes consisting of a single RNA and more than 2100 identical coat protein subunits have enabled molecular studies which have pioneered the understanding of viral replication and self-assembly, and elucidated major aspects of virus–host interplay, which can lead to agronomically relevant diseases. However, during the last decades, TMV has acquired a new reputation as a well-defined high-yield nanotemplate with multivalent protein surfaces, allowing for an ordered high-density presentation of multiple active molecules or synthetic compounds. Amino acid side chains exposed on the viral coat may be tailored genetically or biochemically to meet the demands for selective conjugation reactions, or to directly engineer novel functionality on TMV-derived nanosticks. The natural TMV size (length: 300 nm) in combination with functional ligands such as peptides, enzymes, dyes, drugs or inorganic materials is advantageous for applications ranging from biomedical imaging and therapy approaches over surface enlargement of battery electrodes to the immobilization of enzymes. TMV building blocks are also amenable to external control of in vitro assembly and re-organization into technically expedient new shapes or arrays, which bears a unique potential for the development of 'smart' functional 3D structures. Among those, materials designed for enzyme-based biodetection layouts, which are routinely applied, e.g., for monitoring blood sugar concentrations, might profit particularly from the presence of TMV rods: Their surfaces were recently shown to stabilize enzymatic activities upon repeated consecutive uses and over several weeks. This review gives the reader a ride through strikingly diverse achievements obtained with TMV-based particles, compares them to the progress with related viruses, and focuses on latest results revealing special advantages for enzyme-based biosensing formats, which might be of high interest for diagnostics employing 'systems-on-a-chip'.

scholarly journals Resistance to Soybean Mosaic Virus with High Yield on F7 Soybean Lines

2017 ◽  
Vol 19 (02) ◽  
pp. 226-232 ◽  
Author(s):  
Wuye Ria Andayanie ◽  
Venny Santosa ◽  
Muji Rahayu
Keyword(s):  
Mosaic Virus ◽  
Soybean Mosaic Virus ◽  
High Yield

Red Rider common bea

2009 ◽  
Vol 89 (5) ◽  
pp. 925-927 ◽  
Author(s):  
S J Park ◽  
T Rupert ◽  
K Yu ◽  
A Navabi
Keyword(s):  
Phaseolus Vulgaris ◽  
Mosaic Virus ◽  
Seed Size ◽  
Bean Common Mosaic Virus ◽  
Phaseolus Vulgaris L ◽  
Dry Bean ◽  
Late Season ◽  
Bean Cultivar ◽  
Cultivar Description ◽  
Moderately Resistant

Red Rider is a determinate cranberry bean (Phaseolus vulgaris L.) cultivar with medium-late season maturity. It has relatively tall erect plants without vines and a large seed size (approximately 63.7 g 100 seed-1). Red Rider is moderately resistant to race 15 of bean common mosaic virus. The seed has acceptable cooking and canning quality.Key words: Phaseolus vulgaris L., cranberry bean, dry bean, cultivar description

scholarly journals Patriot – new variety of vegetable pieas

2021 ◽  
pp. 22-25
Author(s):  
A. G. Besedin ◽  
A. V. Tikhonova ◽  
O. V. Putina
Keyword(s):  
High Yield ◽  
New Variety ◽  
Linear Dimensions ◽  
Plot Area ◽  
Green Pea ◽  
Biological Maturity ◽  
Dark Green ◽  
Green Peas ◽  
Selection Of ◽  
Variety Testing

Relevance. The purpose of the research was to produce a new medium-ripe, high-yielding variety of vegetable peas with the usual type of leaf to expand the variety in the now used conveyor variety selection of the Krymsk EBS VIR Branch.Materials and methods. The experience was laid on the fields of the Krymsk EBS VIR Branch (Russia, Krasnodar Region, Krymsk) in 2019-2020. Varieties and strain were evaluated in a competitive variety testing. Sowing was carried out with a selection seeder SKS-6-10. The plot area was 10 m2 , the experiment was repeated four times. In the phase of technical ripeness, the sheaves were selected for description and the yield was taken into account. The conservation of green peas was done at the Tech Evaluation Lab.Results. Competitive variety testing in the mid-ripeness group was held by two strains 2014/7 and 344/16. The strain 2014/17 for green pea yields surpassed the standard "Parus" and G-344/16 in both years of study. Fresh and processed green peas were rated by the Tasting Commission at 5.0 and 4.8 points, respectively. High yield is achieved due to the paired beans on the peduncle and a large number of grains in the bean, up to 10 pieces. The grain in technical ripeness has a dark green color and the linear dimensions are 9.0x8.1 mm. In biological maturity, the seeds are intensely green, angular-compressed with a mass of 1000 pieces. 188-225 g.Conclusion. The results of field and laboratory analyses carried out on the complex of economicvaluable characteristics made it possible to distinguish the strain 2014/7, which in 2020 was transferred to the State Variety Testing under the name "Patriot". It is recommended as a supplement to the existing set of vegetable pea varieties of the Krymsk EBS VIR Branch in the mid-ripe group. 

scholarly journals HERITABILITAS, NISBAH POTENSI, DAN HETEROSIS KETAHANAN KEDELAI (Glycine max [L.] Merrill) TERHADAP SOYBEAN MOSAIC VIRUS

2016 ◽  
Vol 16 (1) ◽  
pp. 17
Author(s):  
Nyimas Sa’diyah ◽  
Hasriadi Mat Akin ◽  
Ria Putri ◽  
Risa Jamil ◽  
Maimun Barmawi
Keyword(s):  
Glycine Max ◽  
Mosaic Virus ◽  
Disease Severity ◽  
Block Design ◽  
Soybean Mosaic Virus ◽  
Mosaic Disease ◽  
Narrow Sense ◽  
Narrow Sense Heritability ◽  
Glycine Max L ◽  
Genetic And Environmental Factors

Heritability, potential ratio, and heterosis of soybean (Glycine max [L.] Merrill) resistance to soybean mosaic virus. The use of soybean cultivars with resistance to SMV is a way for controlling soybean mosaic disease. The objective of this research was to estimate the disease severity, the narrow sense heritability, potential ratio and heterosis of resistance character and number of pithy pods, number of healthy seeds, and healthy seeds weight per plant of ten F1 populations of soybean crossing result to SMV infection. The experiment was arranged in a randomized complete block design in two replications. Observed characters were disease severity, number of pithy pods, number of healthy seeds, and healthy seeds weight per plant. The result of this research showed that 1) the crossing combinations those which were resistant to SMV (lower disease severity) were Yellow Bean x Tanggamus, Tanggamus x Orba, and Tanggamus x Taichung, 2) the narrow sense heritability of disease severity was included in medium criteria, 3) number of pithy pods belonged to high criteria, and 4) number of healthy seeds and healthy seeds weight per plant were included in low criteria. The crossing combinations that had low estimation value of heterosis and heterobeltiosis of resistance to SMV infection were Yellow Bean x Taichung, Bean x Tanggamus and Tanggamus x B3570. Disease severity or resistance to SMV is influenced by genetic and environmental factors.

scholarly journals DIAGNOSIS PENYAKIT MOSAIK (SOYBEAN MOSAIC VIRUS) TERBAWA BENIH KEDELAI

2012 ◽  
Vol 12 (2) ◽  
pp. 185-191
Author(s):  
Wuye Ria Andayanie
Keyword(s):  
Electron Microscopy ◽  
Mosaic Virus ◽  
Soybean Mosaic Virus ◽  
Soybean Seed ◽  
Mosaic Disease ◽  
Leaf Extracts ◽  
Serological Assay ◽  
Visual Symptoms ◽  
Positive Results ◽  
Infectivity Test

Soybean mosaic disease is wide spread throughout soybean-growing countries. Incidence of this disease in East Java is caused by Soybean mosaic virus (SMV) and cowpea mild mottle virus (CMMV).  This aim of study was to find the etiology of  disease at 14-28 days after planting (dap) on soybean.  Research was done by observing visual symptoms. Visual symptoms confirmed by infectivity test, serology assay,electron microscopy (EM) and molecular detection. Results from experiment indicated that soybean plants (14-28 dap) with  symptom  could be detected in infectivity test. Mechanical inoculation with symptomatic leaf extracts produced local lessions on Chenopodium amaranticolor. Positive results were obtained for Madiun, Ngawi, and Magetan samples when tested against antiserum of SMV in serological assay, however Ponorogo samples were not detected by serological assay. Electron microscopy was also done for the selected sample to confirm the result of positive results.  In EM observations, characteristic filamentous particles with modal length close to  900 nm  were observed in samples infected with SMV.  We detected an array of amplification products of expected size 1385 bp fragment of  cylindrical inclusion gene from Madiun, Ngawi, and Magetan isolates in former fields, but not detected in Ponorogo isolate. This result showed the existence of SMV of soybean seed transmission at 14-28 dap in Madiun, Magetan and Ngawi.

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