Uji Efektivitas Ekstrak Etanol Daun Upo-upo (Phyllodium pulchellum (L.) Desv.) sebagai Antibakteri terhadap  Pertumbuhan Streptococcus viridans dan Streptococcus  pyogenes

Dewi Isnaeni; Andi Ulfa Magefirah Rasyid;  Rahmawati

doi:10.24123/mpi.v3i3.3629

Uji Efektivitas Ekstrak Etanol Daun Upo-upo (Phyllodium pulchellum (L.) Desv.) sebagai Antibakteri terhadap Pertumbuhan Streptococcus viridans dan Streptococcus pyogenes

Media Pharmaceutica Indonesiana (MPI) ◽

10.24123/mpi.v3i3.3629 ◽

2021 ◽

Vol 3 (3) ◽

pp. 138-145

Author(s):

Dewi Isnaeni ◽

Andi Ulfa Magefirah Rasyid ◽

Rahmawati

Keyword(s):

Streptococcus Pyogenes ◽

Streptococcus Viridans ◽

Disc Diffusion

Telah dilakukan penelitian untuk menguji efektivitas ekstrak etanol daun Upo-upo (Phyllodium pulchellum (L.) Desv.) terhadap pertumbuhan Streptococcus viridans dan Streptococcus pyogenes. Penelitian ini bertujuan untuk menentukan konsentrasi ekstrak yang paling efektif. Penelitian ini menggunakan metode ekstraksi maserasi terhadap bahan uji dan uji mikrobiologis menggunakan metode Disc diffusion menurut Kirby & Bauer dengan konsentrasi ekstrak 1; 1,5; 2; dan 2,5%, dengan kontrol positif cefixime dan kontrol negatif CMC Na 1%. Hasil pengujian menunjukkan bahwa ekstrak etanol daun Upo-upo dapat menghambat pertumbuhan S. viridans pada konsentrasi ekstrak 1; 1,5; 2; dan 2,5%, masing-masing dengan diameter hambatan 9,01; 9,58; 10,24; dan 12,27 mm, sedangkan zona hambatan untuk kontrol positif 22,07 mm dan kontrol negatif tanpa zona hambatan. Zona hambatan terhadap S. pyogenes pada konsentrasi ekstrak 1; 1,5; 2; dan 2,5% masing-masing adalah 9,51; 9,91; 10,93; dan 13,59 mm, dimana zona hambatan kontrol positif 25,77 mm dan kontrol negatif tanpa zona hambatan. Dapat disimpulkan bahwa ekstrak etanol daun Upo-upo pada semua tingkat konsentrasi mempunyai efektivitas antibakteri terhadap S. viridans dan S. pyogenes. Efektivitas antibakteri ekstrak etanol daun Upo-upo terhadap pertumbuhan S. viridans dan S. pyogenes menunjukkan bahwa semakin tinggi konsentrasi ekstrak, maka semakin besar pula zona hambat yang dihasilkan. Konsentrasi yang paling efektif adalah 2,5%.

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Isolation, Characterization and Antibacterial Activity of Ergosta-5, 7, 22-triene-3β, 14α – diol (22Z) from Kenyan Ganoderma lucidum

Asian Journal of Applied Chemistry Research ◽

10.9734/ajacr/2020/v5i130127 ◽

2020 ◽

pp. 48-57

Author(s):

Ebrahim Sande ◽

Danstone Lilechi Baraza ◽

Selline Ooko ◽

Peter Kuloba Nyongesa

Keyword(s):

Staphylococcus Aureus ◽

Antibacterial Activity ◽

Streptococcus Pyogenes ◽

Ganoderma Lucidum ◽

Methicillin Resistance ◽

Hexane Extract ◽

Diffusion Method ◽

Significant Activity ◽

Disc Diffusion ◽

Disc Diffusion Method

Aims: To determine the chemical composition and antibacterial activity of Kenyan Ganoderma lucidum. Study Design: Structural determination of the isolated compound was done using spectral evidences and in comparison with literature. The antibacterial properties of the compound was done using disc diffusion method. Place and Duration of Study: Department of Pure and Applied Chemistry, Masinde Muliro University of Science and Technology, between January and November, 2019. Methodology: Sequential extraction of dried samples of Kenyan G. lucidum were done using solvents hexane, ethyl acetate and methanol. Chromatographic separation of hexane extract of Ganoderma lucidum was done using spectroscopic data. The compound was assayed against Escherichia coli, Klebsiella pneumoniae, Methicillin–Resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa and Streptococcus pyogenes. Standard antibiotic namely; ampicillin was used as the control. Disc diffusion method was used and zones of inhibition, after respective incubation periods, were used to quantify antibacterial activity. Results: From hexane extract of Ganoderma lucidum, Ergosta-5, 7, 22-triene-3β, 14α – diol (22Z) was isolated. Ethylacetate and methanol extracts produced a mixture of complex compounds. Ergosta-5,7,22-triene-3β,14α-diol (22Z) exhibited significant activity against Methicillin-Resistance Staphylococcus aureus (MRSA) (p=0.022) and Streptococcus pyogenes (p = 0.05). The most sensitive microbe was Streptococcus pyogenes. Conclusion: One major compound, Ergosta-5, 7, 22-triene-3β, 14α – diol (22Z) was isolated, characterized and antibacterial activity determined.

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Antimicrobial Activity of some Honey Samples against Pathogenic Bacteria

International Letters of Natural Sciences ◽

10.18052/www.scipress.com/ilns.34.15 ◽

2015 ◽

Vol 34 ◽

pp. 15-20 ◽

Cited By ~ 4

Author(s):

S. Mahendran ◽

D. Kumarasamy

Keyword(s):

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Antibacterial Activity ◽

Streptococcus Pyogenes ◽

Western Ghats ◽

Pathogenic Bacteria ◽

Research Work ◽

Diffusion Method ◽

Disc Diffusion ◽

Disc Diffusion Method

The aim of the present research work to investigate antimicrobial activity of some honey samples six winter honeys six summer honeys collected from different regions of Western Ghats. The microbes used in this study are Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, Pseudomonas aeruginosa, and Proteus mirabilis. Antibacterial activity of the honeys was assayed using the Disc diffusion method. Noticeable variations in the antibacterial activity of the different honey samples were observed. Among the microbes Staphylococcus aureus is the most sensitive against all honey samples shows the maximum inhibitor zone compare to summer honeys.

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UJI AKTIVITAS ANTIBAKTERI EKSTRAK TERIPANG GAMAT (Stichopus Variegatus) DARI KEPULAUAN MENTAWAI TERHADAP BAKTERI STREPTOCOCCUS VIRIDANS

B-Dent, Jurnal Kedokteran Gigi Universitas Baiturrahmah ◽

10.33854/jbdjbd.89 ◽

2019 ◽

Vol 4 (1) ◽

pp. 52-60

Author(s):

Geistia Fad’ha ◽

Utmi Arma ◽

Busman Busman

Keyword(s):

Streptococcus Viridans ◽

P Value ◽

Disc Diffusion

Kabupaten Kepulauan Mentawai Provinsi Sumatera Barat mempunyai wilayah pantai yang cukup luas dan tersimpan kekayaan laut yang memiliki potensi ekonomi yang tinggi, salah satunya adalah Teripang. Teripang selain memiliki nilai gizi yang tinggi juga mempunyai aktivitas antibakteri terhadap bakteri patogen. Di bidang kedokteran gigi, Streptococcus viridans merupakan salah satu bakteri penyebab utama dalam permasalahan kesehatan gigi di rongga mulut. Bakteri ini merupakan flora normal di rongga mulut yang dapat menyebabkan karies gigi. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak Teripang Gamat (Stichopus variegatus) dari Kepulauan Mentawai terhadap bakteri Streptococcus viridans. Penelitian ini merupakan penelitian eksperimental laboratorium menggunakan metode disc diffusion (tes Kirby & Bauer) yang telah dilakukan pada bulan Januari-Maret 2016 di Laboratorium Biota Sumatera, Universitas Andalas. Hasil penelitian diuji menggunakan Uji ANOVA dengan tingkat signifikan 5% dengan aplikasi SPSS for windows 16.0. Hasil penelitian diketahui rata-rata diameter zona hambat ekstrak Teripang Gamat (Stichopus variegatus) dari Kepulauan Mentawai terhadap bakteri Streptococcus viridans paling tinggi pada konsentrasi 2,5% dengan nilai 8,45 mm yang termasuk kategori sedang. Nilai p-value dari hasil tes uji anova p-value

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Regulation of protein H expression in M1 serotype isolates of Streptococcus pyogenes

FEMS Microbiology Letters ◽

10.1016/s0378-1097(02)01185-9 ◽

2003 ◽

Author(s):

T Smith

Keyword(s):

Streptococcus Pyogenes

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Tonsillar Inflammation, a Rare Cause of Bradycardia and Hypotension

Medical & Clinical Research ◽

10.33140/mcr.03.03.03 ◽

2018 ◽

Vol 3 (3) ◽

Keyword(s):

Streptococcus Pyogenes ◽

Fusobacterium Necrophorum ◽

Outpatient Setting ◽

Peritonsillar Abscess ◽

The Other ◽

Streptococcus Group ◽

Other Hand ◽

Group A

Tonsillitis is a frequently encountered pathology in the outpatient setting, usually caused by viruses [1]. When bacterial, the most common causatory microbe is streptococcus group A [1]. Tonsillar and peritonsillar abscess (PTA) on the other hand are never viral, and are usually caused by streptococcus pyogenes, Streptococcus melleri, fusobacterium necrophorum and staphylococci [1,2]. The overall incidence of PTA is suggested to be 37/100,000 patients, with the highest incidence between ages 14-21 at 124/100,000 [3].

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Decreased carO gene expression and OXA-type carbapenemases among extensively drug-resistant Acinetobacter baumannii strains isolated from burn patients in Tehran, Iran

Acta Microbiologica et Immunologica Hungarica ◽

10.1556/030.2020.01138 ◽

2020 ◽

Cited By ~ 1

Author(s):

Elham Abbasi ◽

Hossein Goudarzi ◽

Ali Hashemi ◽

Alireza Salimi Chirani ◽

Abdollah Ardebili ◽

...

Keyword(s):

Gene Expression ◽

Acinetobacter Baumannii ◽

High Rate ◽

Burn Patients ◽

Drug Resistant ◽

Disc Diffusion ◽

Carbapenem Resistant ◽

Extensively Drug Resistant ◽

Sds Page ◽

Extensively Drug Resistance

AbstractA major challenge in the treatment of infections has been the rise of extensively drug resistance (XDR) and multidrug resistance (MDR) in Acinetobacter baumannii. The goals of this study were to determine the pattern of antimicrobial susceptibility, blaOXA and carO genes among burn-isolated A. baumannii strains. In this study, 100 A. baumannii strains were isolated from burn patients and their susceptibilities to different antibiotics were determined using disc diffusion testing and broth microdilution. Presence of carO gene and OXA-type carbapenemase genes was tested by PCR and sequencing. SDS-PAGE was done to survey CarO porin and the expression level of carO gene was evaluated by Real-Time PCR. A high rate of resistance to meropenem (98%), imipenem (98%) and doripenem (98%) was detected. All tested A. baumannii strains were susceptible to colistin. The results indicated that 84.9% were XDR and 97.9% of strains were MDR. In addition, all strains bore blaOXA-51 like and blaOXA-23 like and carO genes. Nonetheless, blaOXA-58 like and blaOXA-24 like genes were harbored by 0 percent and 76 percent of strains, respectively. The relative expression levels of the carO gene ranged from 0.06 to 35.01 fold lower than that of carbapenem-susceptible A. baumannii ATCC19606 and SDS – PAGE analysis of the outer membrane protein showed that all 100 isolates produced CarO. The results of current study revealed prevalence of blaOXA genes and changes in carO gene expression in carbapenem resistant A.baumannii.

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