Genetic Variability in the Propagation of Eucalyptus sideroxylon by Stem Cuttings

Stem cuttings from mature trees and coppice regrowth of three red ironbark eucalyptus (Eucalyptus sideroxylon A. Cunn. ex Woolls) genotypes were used to study the rooting response over time. Mature tissues failed to root, but coppice regrowth from the same trees showed a range in rooting response from 0 to nearly 100 percent. The two genotypes that formed adventitious roots maintained this ability for the duration of the study. The one genotype that exhibited the greatest potential for adventitious root formation formed a greater number of roots per cutting and had a greater mean root length.

Download Full-text

Effect of Cutting Size and Basal Heat on Rooting of Micromeria fruticulosa Stem Cuttings

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha45210667 ◽

2017 ◽

Vol 45 (2) ◽

pp. 353-357

Author(s):

Leo SABATINO ◽

Fabio D'ANNA ◽

Giovanni IAPICHINO

Keyword(s):

Adventitious Roots ◽

Root Formation ◽

Adventitious Root Formation ◽

Wild Plants ◽

Stem Cuttings ◽

Native Plant ◽

Factors Influencing ◽

Shade Cloth ◽

Propagation Methods ◽

Rooting Response

Micromeria fruticulosa (Bertol.) Grande is a small pulviniform shrub, belonging to the Labiates, and characterized by twisted stems and pink-purple flowers. Endemic to Campania and Sicily (Italy), M. fruticulosa grows from sea level to 600 m altitude. This specie might be considered an excellent native plant for landscape purposes in Mediterranean areas because of its long flowering period, extending from November to June, and its tolerance to heat and drought. The exploitation of wild plants for ornamental purposes implies knowledge on the factors influencing the propagation methods. Root development of stem cuttings of M. fruticulosa was investigated in relation to basal heat and cutting size. Softwood terminal cuttings of a clone grown in Sicily were trimmed to two sizes: short (3 cm) or long (6 cm) length. Propagation was performed in unheated greenhouse covered with clear polyethylene and external 70% shade-cloth. To verify the rooting response to basal heat, half of the cuttings were placed on a basal heated bench (22 ± 2 °C constant temperature) while the remaining were placed on an unheated bench. Acclimatized rooted cuttings were thereafter transplanted into each plastic pot (diameter 16 cm). Plants were thereafter transplanted in the open field and were evaluated for their ornamental value. Basal heat promoted earlier rooting and positively affected adventitious root formation. Six cm long cuttings exposed to basal heat exhibited the best development in terms of number and length of adventitious roots. Flowering plants derived from 6 cm long cuttings exposed to basal heat showed the highest number of flowering branches and flowers per plant.

Download Full-text

Auxin, Wounding, and Propagation Medium Affect Rooting Response of Stem Cuttings of Arbutus andrachne

HortScience ◽

10.21273/hortsci.36.5.976 ◽

2001 ◽

Vol 36 (5) ◽

pp. 976-978 ◽

Cited By ~ 9

Author(s):

Mohammed M. Al-Salem ◽

Nabila S. Karam

Keyword(s):

Root Formation ◽

Adventitious Root Formation ◽

Chemical Form ◽

Naphthaleneacetic Acid ◽

Stem Cuttings ◽

Acid Form ◽

Root Number ◽

Propagation Medium ◽

Salt Forms ◽

Rooting Response

Stem cuttings of Arbutus andrachne L. were taken from basal or terminal portions of branches in October and treated with acid or salt forms of IBA or NAA at several concentrations. Also, the effects of wounding and propagation medium were assessed. Auxin was necessary for root formation, IBA acid being most effective. In general, salt forms of IBA and NAA were less effective than acid forms. Concentration of auxin had a significant effect on rooting regardless of auxin type or chemical form. The greatest rooting percentage, root number, length, and fresh and dry weights were exhibited by basal cuttings treated with 24 mm IBA. Position of the cutting on the branch did not affect rooting except when the IBA acid form was used; basal cuttings were then superior to terminal ones. Wounding, by making two opposite longitudinal incisions at the base of the cutting, increased rooting. The propagation medium had a significant effect on adventitious root formation, which was enhanced with increasing perlite percentage in the medium up to 100%. Chemical names used:1H-indole-3-butyric acid (IBA); α-naphthaleneacetic acid (NAA).

Download Full-text

Highly Successful Adventitious Root Formation of Zamia L. Stem Cuttings Exhibits Minimal Response to Indole-3-Butyric Acid

HortScience ◽

10.21273/hortsci15212-20 ◽

2020 ◽

Vol 55 (9) ◽

pp. 1463-1467 ◽

Cited By ~ 3

Author(s):

Benjamin E. Deloso ◽

Anders J. Lindström ◽

Frank A. Camacho ◽

Thomas E. Marler

Keyword(s):

Butyric Acid ◽

Root Development ◽

Adventitious Root ◽

Adventitious Roots ◽

Root Formation ◽

Adventitious Root Formation ◽

The Other ◽

Control Group ◽

Stem Cuttings ◽

Adventitious Root Development

The influences of indole-3-butyric acid (IBA) concentrations of 0–30 mg·g−1 on the success and speed of adventitious root development of Zamia furfuracea L.f. and Zamia integrifolia L.f. stem cuttings were determined. Root formation success for both species was greater than 95%. The IBA concentrations did not influence the speed of root development for Z. furfuracea, but the Z. integrifolia cuttings that received IBA concentration of 3 mg·g−1 generated adventitious roots more slowly than the cuttings in the control group. The ending dry weights of the stems, leaves, and roots were not influenced by IBA concentration for either species. Our results indicated that adventitious root formation on stem cuttings of these two Zamia species is successful without horticultural application of IBA. Additional IBA studies are needed on the other 300+ cycad species, especially those that are in a threatened category.

Download Full-text

Anatomy of Adventitious Root Formation in Stem Cuttings of Mussaenda erythrophylla L. `Rosea'

HortScience ◽

10.21273/hortsci.31.4.634b ◽

1996 ◽

Vol 31 (4) ◽

pp. 634b-634

Author(s):

Rolston St. Hilaire ◽

Carlos A. Fierro Berwart

Keyword(s):

Adventitious Root ◽

Adventitious Roots ◽

Root Formation ◽

Adventitious Root Formation ◽

Stem Cuttings ◽

Root Primordia ◽

Fast Green ◽

Phloem Parenchyma ◽

Ornamental Shrubs ◽

Vascular Connections

Mussaendas (Mussaenda spp.) are ornamental shrubs, and some cultivars are difficult to root. This study was conducted to explore how adventitious roots initiate and develop in the cultivar Rosea, and to determine if anatomical events are associated with difficulty in rooting stem cuttings. Stem cuttings were treated with 5, 10, 15 mm 1H-indole-3-butyric acid (IBA), or distilled water, and sampled every 2 days over 26 days to observe adventitious root formation and development. For analysis by light microscopy, the basal 1 cm of cuttings was embedded in wax and stained with safranin-fast green. Adventitious roots initiated from phloem parenchyma cells and from basal callus in nontreated cuttings. Cuttings treated with 15 mm IBA had a mean of 18 root primordia per basal 1 cm of cutting after 10 days. Root primordia were not observed in non-treated cuttings at 10 days. Root primordia that developed in non-treated cuttings lacked clear vascular connections. These results suggest that non-treated cuttings are difficult to root because few primordia are produced.

Download Full-text

INITIATION AND DEVELOPMENT OF ADVENTITIOUS ROOTS IN STEM CUTTINGS OF HEDERA HELIX: ANATOMICAL STUDIES OF THE MATURE GROWTH PHASE

Canadian Journal of Botany ◽

10.1139/b67-203 ◽

1967 ◽

Vol 45 (10) ◽

pp. 1883-1886 ◽

Cited By ~ 7

Author(s):

Ronald M. Girouard

Keyword(s):

Growth Phase ◽

Adventitious Root ◽

Adventitious Roots ◽

Root Formation ◽

Adventitious Root Formation ◽

Stem Cuttings ◽

Hedera Helix ◽

Anatomical Studies ◽

Ray Parenchyma ◽

English Ivy

Adventitious root formation was studied microscopically in stem cuttings of a difficult-to-root plant, the mature growth phase of Hedera helix, English ivy. Roots were found initiating at the end of 2–4 weeks in phloem ray parenchyma of internodes with and without woundwood and in callus near the basal end of cuttings; roots emerged at the end of 3–5 weeks. The fibro-bundle caps were thicker than those found in juvenile stems, but they did not hamper the development of adventitious roots.

Download Full-text

INITIATION AND DEVELOPMENT OF ADVENTITIOUS ROOTS IN STEM CUTTINGS OF HEDERA HELIX: ANATOMICAL STUDIES OF THE JUVENILE GROWTH PHASE

Canadian Journal of Botany ◽

10.1139/b67-202 ◽

1967 ◽

Vol 45 (10) ◽

pp. 1877-1881 ◽

Cited By ~ 14

Author(s):

Ronald M. Girouard

Keyword(s):

Growth Phase ◽

Adventitious Roots ◽

Root Formation ◽

Adventitious Root Formation ◽

Main Axis ◽

Juvenile Growth ◽

Stem Cuttings ◽

Hedera Helix ◽

Anatomical Studies ◽

Ray Parenchyma

Anatomical studies were made of adventitious root formation in the internodes of cuttings from juvenile phase plants of Hedera helix, English ivy. Observations showed that adventitious roots originate in phloem ray parenchyma above the basal cuts within a period of 6–10 days. The actual emergence of the roots is at right angles to the main axis of the stems and the roots appear 10–14 days after the cuttings are made. Fibers are present in groups external and tangential to the phloem strands, but they do not inhibit development of the roots.

Download Full-text

Adventitious Root Formation and Development in Cuttings of Mussaenda erythrophylla L. Schum. & Thonn.

HortScience ◽

10.21273/hortsci.31.6.1023 ◽

1996 ◽

Vol 31 (6) ◽

pp. 1023-1025 ◽

Cited By ~ 8

Author(s):

Rolston St. Hilaire ◽

Carlos A. Fierro Berwart ◽

Carlos A. Pérez-Muñoz

Keyword(s):

Adventitious Root ◽

Adventitious Roots ◽

Root Formation ◽

Adventitious Root Formation ◽

Distilled Water ◽

Stem Cuttings ◽

Root Primordia ◽

Phloem Parenchyma ◽

Parenchyma Cells ◽

Ornamental Shrubs

Mussaendas (Mussaenda spp.) are ornamental shrubs, and some cultivars are difficult to root. This study was conducted to explore how adventitious roots initiate and develop in the cultivar Rosea and to determine if anatomical events are associated with difficulty in rooting stem cuttings. Stem cuttings were treated with 5, 10, 15 mmol IBA, or distilled water, and sampled every 2 days over 26 days to observe adventitious root formation and development. Adventitious roots initiated from phloem parenchyma cells. Cuttings treated with 15 mmol IBA had a mean of 18 root primordia per basal 1 cm of cutting after 10 days. Primordia were absent in nontreated cuttings at 10 days. These results suggest that nontreated cuttings are difficult to root because few primordia are produced. Chemical name used: 1H-indole-3-butyric acid (IBA).

Download Full-text