scholarly journals Essential Oil Composition, Total Phenolic Content, and Antioxidant Activity - Determined from Leaves, Flowers and Stems of Origanum Vulgare L. Var. Aureum

2018 ◽  
Vol 1 (1) ◽  
pp. 555-561
Author(s):  
Cristian Moisa ◽  
Lucian Copolovici ◽  
Georgeta Pop ◽  
Andreea Lupitu ◽  
Virgiliu Ciutina ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Essential Oils ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Biologically Active ◽  
Total Phenolic ◽  
Alcoholic Extract ◽  
Origanum Vulgare ◽  
Specific Chemical

Abstract Origanum vulgare L. var. aureum is an aromatic medicinal plant belonging to the Lamiaceae family, rich in essential oils and antioxidants. Its specific chemical composition represents an important source of biologically active principles with diverse applications in food products and nutraceuticals. The aim of this study was to determine the differences between chemical composition of volatile oils, phenolic content and antioxidant activity of different plants parts: leaves, flowers, and stems. Essential oils from leaves and flowers had similar compositions with only few differences between γ-terpinene and trans-ß-ocimene. Total phenolic content and antioxidant activity were determined for the hydro-alcoholic extract obtained from post-distillation oregano waste material. The total phenolic content was determined using Folin-Ciocalteu reagent and varied from 3173 to 6522 mg GAE/L. The major compounds identified by HPLC were syringic acid, pyrocatechol, and catechin. Furthermore, all extracts showed high antioxidant activity, ranging from 31.3 to 44.5 mg GAE/L, with an inhibition percent varying from 63.1 to 88.6%.

scholarly journals Chemical and Biochemical Characterization of Essential Oils and Their Corresponding Hydrolats from Six Species of the Lamiaceae Family

Plants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2489
Author(s):  
Cristina Laura Popa ◽  
Andreea Lupitu ◽  
Maria Daniela Mot ◽  
Lucian Copolovici ◽  
Cristian Moisa ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Essential Oils ◽  
Total Phenolic Content ◽  
Biochemical Characterization ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Thymus Vulgaris ◽  
Origanum Vulgare ◽  
Lavandula Angustifolia ◽  
Food And Beverage

Many plants belonging to the Lamiaceae family are rich in essential oils (EOs) which are intensively used for aromatherapy, food and beverage flavoring, alternative medicine, cosmetics, and perfumery. Aerial parts of Thymus vulgaris L., Thymus pannonicus All., Lavandula angustifolia L., Lavandula x intermedia, Origanum vulgare L., and Origanum vulgare var. aureum L. were subjected to hydrodistillation, and both resulting fractions were analyzed. The purpose of this study was to determine the chemical composition, antioxidant activity, and total phenolic content of six essential oils and their corresponding hydrolats (HDs) through GC-MS and spectrophotometric analyses. Overall, 161 compounds were identified, some found exclusively in essential oils and others in hydrolats, making them individual products with specific end purposes. The total phenolic content was the highest for the Thymus vulgaris L. EOs (3022 ± mg GAE L−1), because of its high phenolic oxygenated monoterpenes content (thymol and carvacrol) and the smallest for the Lavandula angustifolia L. EOs (258.31 ± 44.29 mg GAE L−1), while hydrolats varied from 183.85 ± 0.22 mg GAE L−1 for Thymus vulgaris L. HD and 7.73 mg GAE L−1 for Thymus pannonicus All. HD. Significant antioxidant effects determined through DPPH• and ABTS•+ assays were also observed in samples with higher hydrophilic compounds. The highest antioxidant activity was determined for Thymus vulgaris L. EO and its corresponding HD. Although EOs are the principal traded economic product, HDs represent a valuable by-product that could still present intense antiseptic activities, similar to their corresponding EOs (thyme and oregano), or have multiple aromatherapy, cosmetics, and household applications (lavender and lavandin).

scholarly journals Seasonal Variation in the Chemical Composition, Antioxidant Activity and Total Phenolic Content of Teucrium persicum Boiss. Essential Oils

2010 ◽  
Vol 5 (7) ◽  
pp. 492-498 ◽  
Author(s):  
Hamid Reza Monsef-Esf ◽  
Abdolhossein Miri ◽  
Mohsen Amini ◽  
Yaghoub Amanzadeh ◽  
Abbas Hadjiakhoo ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Seasonal Variation ◽  
Chemical Composition ◽  
Essential Oils ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic

scholarly journals Exploration of Productivity, Chemical Composition, and Antioxidant Potential of Origanum vulgare L. Grown at Different Geographical Locations of Western Himalaya, India

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Shweta Goyal ◽  
Geeta Tewari ◽  
H. K Pandey ◽  
Anjali Kumari
Keyword(s):  
Antioxidant Activity ◽  
Essential Oils ◽  
Reducing Power ◽  
Western Himalaya ◽  
Antioxidant Potential ◽  
Aluminium Chloride ◽  
Total Phenolic ◽  
Alcoholic Extract ◽  
Origanum Vulgare ◽  
Field Station

Origanum vulgare L., the most complex and variable species of the genus Origanum, is used to cure various diseases in the traditional medicinal system. In the current investigation, O. vulgare was collected from its natural origin and further multiplied for two years at the experimental farm of DIBER field station, Auli, and afterwards, it was grown at three different altitudes, i.e., DIBER field station of Auli (2744 m), Pithoragarh (1524 m), and Haldwani (412 m), under natural field conditions. The essential oil was extracted using the Clevenger apparatus and analyzed by GC/MS and GC/FID. The total phenolic, flavonoid, and tannin content in the methanolic extracts of all the cultivations were estimated by using Folin–Ciocalteu reagent, aluminium chloride reagent, and Folin–Denis methods, respectively. The antioxidant activity of the essential oils and extracts of all the cultivations was also estimated by DPPH, ABTS, and reducing power methods. The highest amount of phenolics, flavonoids, and tannins was obtained in the Pithoragarh cultivation. Thymol was the major component in all the essential oils contributing 38.81% (Auli), 52.83% (Pithoragarh), and 31.86% (Haldwani) of the total oil. The highest antioxidant activity was exhibited by oregano oil of Pithoragarh cultivation, followed by Auli and Haldwani, respectively. The alcoholic extract of Pithoragarh cultivation had the highest antioxidant activity. The study infers that the plant demonstrated good results in terms of fresh weight, oil yield, antioxidant potential, and EO composition when grown at higher altitudes.

scholarly journals Chemical composition and antioxidant activity of geopropolis produced by Melipona fasciculata (Meliponinae) in flooded fields and cerrado areas of Maranhão State, northeastern Brazil

2016 ◽  
Vol 46 (3) ◽  
pp. 315-322 ◽  
Author(s):  
Marisa Cristina Aranha BATISTA ◽  
Bruno Vinicius de Barros ABREU ◽  
Richard Pereira DUTRA ◽  
Mayara Soares CUNHA ◽  
Flavia Maria Mendonça do AMARAL ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Gallic Acid ◽  
Phenolic Acids ◽  
Ellagic Acid ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Northeastern Brazil ◽  
Total Phenolic ◽  
Antioxidant Power

ABSTRACT Geopropolis, a mixture of plant resin, wax, soil and salivary secretion, is produced by the stingless bee Melipona fasciculata. This aim of this study was to investigate the chemical composition and antioxidant activity of geopropolis collected from beehives in two phytogeographical regions, flooded fields and cerrado, in the municipalities of Palmeirândia and Fernando Falcão, Maranhão State, northeastern Brazil. The geopropolis compounds were identified by gas chromatography-mass spectrometry (GC/MS). Additionally, total phenolic content was determined with the Folin−Ciocalteu reagent and antioxidant activity was evaluated in vitro by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays. The four geopropolis samples varied in terms of total phenolic content and antioxidant activity and the highest values were observed for geopropolis from Fernando Falcão. Triterpenes such as cycloartane, ursane and oleanane and phenolic acids (protocatechuic acid and gallic acid) were identified in the geopropolis from Palmeirândia, while the phenolic acids, gallic and ellagic acid were the main compounds in geopropolis from Fernando Falcão. The antioxidant property of geopropolis is due to its high total phenolic content and predominance of gallic acid and ellagic acid. The results showed that the plant resources in two phytogeographical regions of Maranhão used by bees for the production of geopropolis contribute to the chemical composition and bioactivity of this product.

scholarly journals Chemical Composition, Antioxidant and Cytotoxicity Activities of Leaves, Bark, Twigs and Oleo-Resin of Dipterocarpus alatus

Molecules ◽  
2019 ◽  
Vol 24 (17) ◽  
pp. 3083 ◽  
Author(s):  
Chawalit Yongram ◽  
Bunleu Sungthong ◽  
Ploenthip Puthongking ◽  
Natthida Weerapreeyakul
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Cytotoxic Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Composition Analysis ◽  
Cytotoxic Activities ◽  
Antioxidant Power ◽  
Dipterocarpus Alatus

Dipterocarpus alatus (Dipterocarpaceae) is a medicinal plant whose use is well known for the treatment of genito-urinary diseases. However, there is no report of its cytotoxic potential. In this study, the chemical composition, antioxidant and cytotoxic activities of extracts of the leaves, bark, twigs and oleo-resin from D. alatus are investigated. Cytotoxicity was measured by the neutral red (NR) assay against HCT116, SKLU1, SK-MEL2, SiHa and U937 cancer cell lines and antioxidant capacity was evaluated by DPPH, ABTS radical scavenging, and ferric reducing antioxidant power (FRAP) assays. The chemical composition was analyzed by gas chromatography–mass spectrometry (GC-MS). Leaf, bark and twig extracts exhibited stronger antioxidant activity than oleo-resin, with bark extract showing the highest antioxidant activity and the highest total phenolic content. All samples showed more cytotoxic activity against the U937 cell line than HCT116, SKLU1, SK-MEL2 and SiHa cells with oleo-resin being more cytotoxic than melphalan against U937 cells. Chemical composition analysis of oleo-resin by GC-MS showed that the major components were sesquiterpenes, namely α-gurjunene (30.31%), (-)-isoledene (13.69%), alloaromadendrene (3.28%), β-caryophyllene (3.14%), γ-gurjunene (3.14%) and spathulenol (1.11%). The cytotoxic activity of oleo-resin can be attributed to the sesquiterpene content, whereas the cytotoxic and antioxidant activities of leaf, bark and twig extracts correlated to total phenolic content.

scholarly journals Total phenolic content, in vitro antioxidant activity and chemical composition of plant extracts from semiarid Mexican region

2015 ◽  
Vol 8 (2) ◽  
pp. 104-111 ◽  
Author(s):  
Jorge E. Wong-Paz ◽  
Juan C. Contreras-Esquivel ◽  
Raúl Rodríguez-Herrera ◽  
María L. Carrillo-Inungaray ◽  
Lluvia I. López ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Chemical Composition ◽  
Plant Extracts ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
In Vitro Antioxidant Activity

scholarly journals Phytochemical Screening and Evaluation of Antioxidant Activity of hydroalcoholic extract of Justicia procumbans leaf

2021 ◽  
Vol 7 (1) ◽  
pp. 41-45
Author(s):  
MY Agrawal ◽  
◽  
YP Agrawal ◽  
SK Arora ◽  
P Lahange ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Assay Method ◽  
Total Phenolic ◽  
Alcoholic Extract ◽  
Dependent Manner ◽  
Phytochemical Screening ◽  
Hydroalcoholic Extract

Objective: To perform Phytochemical Screening and Evaluation of Antioxidant Activity of hydroalcoholic extract Justicia procumbans leaf. Methods: Proximate analysis in terms of ash value, extractive value was performed as per the standard method. Presence of secondary metabolites in the extract of Justicia procumbans was performed through various chemical tests. Folin-Ciocalteu assay method was used to determine total phenolic content and the in-vitro antioxidant activity was investigated in a dose-dependent manner with the 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and hydrogen peroxide free radical scavenging method. Results: Present studies revealed that the hydro-alcoholic extract of leaf contains secondary metabolites such as alkaloids, steroids, flavonoids, carbohydrates, proteins, and tannins. Total phenolic content was found to be 79.32±0.02 mg/gm by using the Linear Equation. IC50 value by DPPH and H2O2 methods was found to be 68.83µg/ml and 56.02 µg/ml respectively. Conclusion: It has been observed that the plant has high phenolic compounds and antioxidant activity. Therefore his plant can be the potent source of natural antioxidants as compared to synthetic compounds.

scholarly journals Antioxidant activity and total phenolic content of essential oils and extracts of sweet basil (Ocimum basilicum L.) plants

2019 ◽  
Vol 8 (3) ◽  
pp. 299-305 ◽  
Author(s):  
Adel F. Ahmed ◽  
Fatma A.K. Attia ◽  
Zhenhua Liu ◽  
Changqin Li ◽  
Jinfeng Wei ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Essential Oils ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Ocimum Basilicum ◽  
Total Phenolic ◽  
Sweet Basil
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