Fasciola hepatica isolates induce different immune responses in unmaturated bovine macrophages

AbstractIntroduction:Fasciola hepatica(liver fluke) is a parasite of great socioeconomic importance. A number of fluke isolates have been identified; however, to date the differences between the immunomodulatory properties of different parasite isolates have not been sufficiently investigated. The aim of this study was to explore differences between the immunomodulatory properties of twoF. hepaticaisolates using unmaturated bovine macrophages.Material and Methods:A cell line of bovine macrophages was stimulated with excretory/secretory products released by adult flukes from either a laboratory (Fh-WeyES) or wild (Fh-WildES) strain and subsequently subjected to microarray and ELISA analyses.Results:BothFh-WeyES andFh-WildES dampened the release of interleukin-10 by bovine macrophages, but onlyFh-WildES dampened the release of proinflammatory tumour necrosis factor-α. Microarray analysis revealed thatFh-WildES down- and upregulated 90 and 18 genes, respectively, when compared toFh-WeyES.Conclusion:The results indicated different impacts of the isolates on macrophages. A number of researchers use flukes obtained from local slaughterhouses for experiments. Our findings may explain some discrepancies between published results arising from parasite strain choice. The findings indicate that consideration should be given to the use of different strains, and open new and currently unexplored avenues in parasitology for controlling the parasite.

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Targeted Deletion of Class A Macrophage Scavenger Receptor Increases the Risk of Cardiac Rupture After Experimental Myocardial Infarction

Circulation ◽

10.1161/circulationaha.106.671198 ◽

2007 ◽

Vol 115 (14) ◽

pp. 1904-1911 ◽

Cited By ~ 50

Author(s):

Kenichi Tsujita ◽

Koichi Kaikita ◽

Takanori Hayasaki ◽

Tsuyoshi Honda ◽

Hironori Kobayashi ◽

...

Keyword(s):

Myocardial Infarction ◽

Tumor Necrosis Factor ◽

Tumor Necrosis ◽

Tumor Necrosis Factor Α ◽

Interleukin 10 ◽

Experimental Myocardial Infarction ◽

Cardiac Rupture ◽

Infarcted Myocardium ◽

Factor Α ◽

Necrosis Factor

Background— Class A macrophage scavenger receptor (SR-A) is a macrophage-restricted multifunctional molecule that optimizes the inflammatory response by modulation of the activity of inflammatory cytokines. This study was conducted with SR-A–deficient (SR-A −/− ) mice to evaluate the relationship between SR-A and cardiac remodeling after myocardial infarction. Methods and Results— Experimental myocardial infarction (MI) was produced by ligation of the left coronary artery in SR-A −/− and wild-type (WT) male mice. The number of mice that died within 4 weeks after MI was significantly greater in SR-A −/− mice than in WT mice ( P =0.03). Importantly, death caused by cardiac rupture within 1 week after MI was 31% (17 of 54 mice) in SR-A −/− mice and 12% (6 of 51 mice) in WT mice ( P =0.01). In situ zymography demonstrated augmented gelatinolytic activity in the infarcted myocardium in SR-A −/− mice compared with WT mice. Real-time reverse transcription–polymerase chain reaction at day 3 after MI showed that the expression of matrix metalloproteinase-9 mRNA increased significantly in the infarcted myocardium in SR-A −/− mice compared with WT mice. Furthermore, SR-A −/− mice showed augmented expression of tumor necrosis factor-α and reduction of interleukin-10 in the infarcted myocardium at day 3 after MI. In vitro experiments also demonstrated increased tumor necrosis factor-α and decreased interleukin-10 expression in activated SR-A −/− macrophages. Conclusions— The present findings suggest that SR-A deficiency might cause impairment of infarct remodeling that results in cardiac rupture via insufficient production of interleukin-10 and enhanced expression of tumor necrosis factor-α and of matrix metalloproteinase-9. SR-A might contribute to the prevention of cardiac rupture after MI.

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Lipopolysaccharide-induced interleukin-10 in mice: role of endogenous tumor necrosis factor-α

European Journal of Immunology ◽

10.1002/eji.1830251027 ◽

1995 ◽

Vol 25 (10) ◽

pp. 2888-2893 ◽

Cited By ~ 81

Author(s):

Johannes Barsig ◽

Sabine Küsters ◽

Kathrin Vogt ◽

Hans-Dieter Volk ◽

Gisa Tiegs ◽

...

Keyword(s):

Tumor Necrosis Factor ◽

Tumor Necrosis ◽

Tumor Necrosis Factor Α ◽

Interleukin 10 ◽

Factor Α ◽

Necrosis Factor

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Polymorphisms of transporter associated with antigen presentation, tumor necrosis factor-α and interleukin-10 and their implications for protection and susceptibility to severe forms of dengue fever in patients in Sri Lanka

Journal of Global Infectious Diseases ◽

10.4103/0974-777x.170501 ◽

2015 ◽

Vol 7 (4) ◽

pp. 157 ◽

Cited By ~ 10

Author(s):

ArunaDharshan De Silva ◽

AniraN Fernando ◽

GathsaurieNeelika Malavige ◽

Kuda LiyanageNandika Perera ◽

Sunil Premawansa ◽

...

Keyword(s):

Tumor Necrosis Factor ◽

Sri Lanka ◽

Antigen Presentation ◽

Dengue Fever ◽

Tumor Necrosis ◽

Tumor Necrosis Factor Α ◽

Interleukin 10 ◽

Factor Α ◽

Necrosis Factor

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Assessment of hepatitis B virus genotype D and interleukin-10, interferon gamma, and tumor necrosis factor-α in fulminant hepatic failure

CHRISMED Journal of Health and Research ◽

10.4103/cjhr.cjhr_131_18 ◽

2019 ◽

Vol 6 (3) ◽

pp. 162

Author(s):

Hiba Sami ◽

Meher Rizvi ◽

Mohd Azam ◽

Indu Shukla ◽

HarisM Khan ◽

...

Keyword(s):

Hepatitis B Virus ◽

Tumor Necrosis Factor ◽

Hepatitis B ◽

Tumor Necrosis ◽

Tumor Necrosis Factor Α ◽

Interleukin 10 ◽

Virus Genotype ◽

B Virus ◽

Factor Α ◽

Necrosis Factor

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Tumour necrosis factor-α/interleukin-10 ratio in patients with obstructive sleep apnoea hypopnoea syndrome

The Journal of Laryngology & Otology ◽

10.1017/s0022215114002990 ◽

2014 ◽

Vol 129 (1) ◽

pp. 73-78 ◽

Cited By ~ 11

Author(s):

H Jiang ◽

H Cao ◽

P Wang ◽

W Liu ◽

F Cao ◽

...

Keyword(s):

Tumour Necrosis Factor ◽

Obstructive Sleep Apnoea ◽

Tumour Necrosis ◽

Positive Airway Pressure ◽

Interleukin 10 ◽

Sleep Apnoea ◽

Tumour Necrosis Factor Α ◽

Obstructive Sleep ◽

Factor Α ◽

Necrosis Factor

AbstractObjective:To explore the significance of the tumour necrosis factor-α/interleukin-10 ratio and the effect of continuous positive airway pressure in patients with different degrees of obstructive sleep apnoea hypopnoea syndrome severity.Method:This study comprised 135 patients with obstructive sleep apnoea hypopnoea syndrome and 94 control subjects.Results:Tumour necrosis factor-α and tumour necrosis factor-α/interleukin-10 ratio values were significantly higher in the obstructive sleep apnoea hypopnoea syndrome group than in the control group, but interleukin-10 was significantly lower. Tumour necrosis factor-α/interleukin-10 ratio values increased in line with the severity of obstructive sleep apnoea hypopnoea syndrome. In multivariate analysis, the tumour necrosis factor-α/interleukin-10 ratio correlated positively with the apnoea–hypopnoea index and all indices of obstructive sleep apnoea hypopnoea syndrome, except for age, body mass index and neck circumference. After one month of continuous positive airway pressure therapy, levels of tumour necrosis factor-α decreased; interleukin-10 showed no change.Conclusion:The results suggest that inflammation is activated and anti-inflammatory cytokines are decreased in obstructive sleep apnoea hypopnoea syndrome patients. Tumour necrosis factor-α/interleukin-10 ratio may prove useful for severity monitoring and management of obstructive sleep apnoea hypopnoea syndrome patients, and may reduce the need for polysomnography.

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Predisposition to Hyperthyroidism May Be Influenced by Functional TNF-α, IL-1, IL-6, and IL-10 Polymorphisms: A Meta-Analysis

International Archives of Allergy and Immunology ◽

10.1159/000508284 ◽

2020 ◽

Vol 181 (12) ◽

pp. 956-965

Author(s):

Hong Ma ◽

Ting Tan ◽

Jie Wu ◽

Juan Chen ◽

Xiaohong Zhang

Keyword(s):

Meta Analysis ◽

Interleukin 1 ◽

Interleukin 10 ◽

Interleukin 4 ◽

Asian Origin ◽

Tnf Α ◽

Caucasian Origin ◽

Meta Analyses ◽

Factor Α ◽

Necrosis Factor

Background: Predisposition to hyperthyroidism may be influenced by functional gene polymorphisms in tumor necrosis factor-α (TNF-α), interleukin-1 (IL-1), interleukin-4 (IL-4), interleukin-6 (IL-6), and interleukin-10 (IL-10). However, the results of the studies published so far remain discrepant, so we conducted a meta-analysis to more robustly investigate relationships between TNF-α/IL-1/IL-4/IL-6/IL-10 polymorphisms and predisposition to hyperthyroidism. Methods: A comprehensive literature retrieval from PubMed, Embase, Web of Science, WanFang, VIP, and CNKI was endorsed by the authors, and 38 studies were found to be eligible for pooled meta-analyses. Results: We found that genotypic frequencies of TNF-α −308 G/A, IL-1A −889 C/T, IL-6 −174 G/C, IL-6 −572 G/C, IL-10 −819 C/T, and IL-10 −1082 A/G polymorphisms among cases were significantly different from those among controls. Moreover, we also found that genotypic frequencies of TNF-α −308 G/A and IL-6 −174 G/C polymorphisms among cases of Caucasian origin were significantly different from those among Caucasian controls, and genotypic frequencies of IL-1A −889 C/T, IL-1B −511 C/T, IL-6 −174 G/C, IL-6 −572 G/C, and IL-10 −1,082 A/G polymorphisms among cases of Asian origin were also significantly different from those among Asian controls. Conclusions: This meta-analysis suggests that TNF-α −308 G/A, IL-1A −889 C/T, IL-1B −511 C/T, IL-6 −174 G/C, IL-6 −572 G/C, IL-10 −819 C/T, and IL-10 −1,082 A/G polymorphisms may influence predisposition to hyperthyroidism in certain ethnic groups.

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