scholarly journals The markers of stress in swine oral fluid

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Marta Giergiel ◽  
Małgorzata Olejnik ◽  
Artur Jabłoński ◽  
Andrzej Posyniak
Keyword(s):  
Oral Fluid ◽  
Acute Stress ◽  
Statistical Significance ◽  
Alpha Amylase ◽  
Control Group ◽  
Protein Markers ◽  
Control Groups ◽  
Total Antioxidant ◽  
Acute Stress Response ◽  
And Control

Abstract Introduction The study measured the hormonal and protein markers of acute stress, those of oxidative stress and total antioxidant capacity (TAC) in swine oral fluid, determined which of these parameters would be the most appropriate for future livestock welfare assessment and established the time when the samples should be taken. Material and Methods Stress was induced in 7 out of 14 castrated six-week-old Danbred×Duroc pigs by immobilisation on a nasal snare at 8 a.m., 1 p.m., and 6 p.m. and samples were taken both directly after the stressor was applied and 30 min later. The remaining pigs were the control group, which were not immobilised; their samples were taken at the same times. The concentrations of hormones and malondialdehyde (MDA) were measured using liquid chromatography with tandem mass spectrometry, while those of alpha-amylase and TAC were measured using spectrophotometry. Results The levels of cortisol and cortisone increased with statistical significance immediately after the acute stress response and 30 min later. A cut-off value set at 0.25 ng/mL cortisol concentration was capable of distinguishing between the stressed and control groups with 100% accuracy in evening samples and 95% accuracy overall. Prednisolone was not present, and the levels of testosterone and corticosterone were low and not distinctive. Alpha-amylase became significantly more concentrated during stress induction and 30 min later. The TAC and MDA levels rose after the stress but without statistical significance. Conclusion The most suitable markers of acute stress were cortisol, cortisone and alpha-amylase. Oral fluid is a reliable material for monitoring the level of pigs’ stress and should be collected in the evening.

scholarly journals Impact of ascorbic acid in reducing the incidence of vancomycin associated nephrotoxicity in critically ill patients: A preliminary randomized controlled trial

F1000Research ◽  
2021 ◽  
Vol 10 ◽  
pp. 929
Author(s):  
Nouran Hesham El-Sherazy ◽  
Naglaa Samir Bazan ◽  
Sara Mahmoud Shaheen ◽  
Nagwa A. Sabri
Keyword(s):  
Ascorbic Acid ◽  
Critically Ill ◽  
Critically Ill Patients ◽  
Statistical Significance ◽  
Intervention Group ◽  
Secondary Outcome ◽  
Control Group ◽  
Control Groups ◽  
The Absolute ◽  
And Control

Background Antioxidants show nephroprotective effect against vancomycin associated nephrotoxicity (VAN) in animals. This study aimed to assess the ascorbic acid nephro-protective role against VAN clinically. Methods Forty-one critically ill patients were randomly assigned to one of two groups: intervention group (vancomycin IV plus ascorbic acid, n=21) or control group (vancomycin IV only, n=20). Primary outcomes were the incidence of VAN and the absolute change in creatinine parameters, while mortality rate was the secondary outcome. Nephrotoxicity was defined as an increase in serum creatinine (S.cr) by at least 0.5 mg/dL or 50% of baseline for at least two successive measurements. This study is registered at Clinicaltrials.gov (NCT03921099), April 2019. Results Mean absolute S.cr increase was significant when compared between both groups, P-value = 0.036, where S.cr increased by 0.05(0.12) and 0.34(0.55) mg/dL in the intervention and control groups, respectively. Mean absolute Cr.cl decline was significant when compared between both groups, P-value = 0.04, where Cr.cl was decreased by 5.9(17.8) and 22.3(30.4) ml/min in the intervention and control groups, respectively. Incidence of VAN was 1/21(4.7%) versus 5/20(25%) in the intervention and control groups, respectively (RR: 0.19; CI: 0.024–1.49; P-value = 0.093). Mortality was higher in the control group; however, it was not statistically significant, P-value = 0.141. Conclusion Co-administration of ascorbic acid with vancomycin preserved renal function and reduced the absolute risk of VAN by 20.3%, however, the reduction in VAN incidence didn’t reach statistical significance level. Further large multicenter prospective trials are recommended.

scholarly journals Reduction of Escherichia coli O157:H7 Populations in Cattle by Addition of Colicin E7-Producing E. coli to Feed

2004 ◽  
Vol 70 (10) ◽  
pp. 6053-6060 ◽  
Author(s):  
Gerry P. Schamberger ◽  
Ronald L. Phillips ◽  
Jennifer L. Jacobs ◽  
Francisco Diez-Gonzalez
Keyword(s):  
Escherichia Coli ◽  
Statistical Significance ◽  
Control Group ◽  
Control Groups ◽  
Tissue Samples ◽  
Fecal Shedding ◽  
E Coli ◽  
Colicin E7 ◽  
Maximum Decrease ◽  
And Control

ABSTRACT A cattle trial using artificially inoculated calves was conducted to determine the effect of the addition of colicinogenic Escherichia coli strains capable of producing colicin E7 (a 61-kDa DNase) to feed on the fecal shedding of serotype O157:H7. The experiment was divided into three periods. In period 1, which lasted 24 days, six calves were used as controls, and eight calves received 107 CFU of E. coli (a mixture of eight colicinogenic E. coli strains) per g of feed. Both groups were orally inoculated with nalidixic acid-resistant E. coli O157:H7 strains 7 days after the treatment started. In periods 2 and 3, the treatment and control groups were switched, and the colicinogenic E. coli dose was increased 10-fold. During period 3, which lasted as long as period 1, both groups were reinoculated with E. coli O157:H7. The numbers of E. coli O157:H7 were consistently greater in the control groups during the three periods, but comparisons within each time period determined a statistically significant (P < 0.05) difference only at day 21 of period 1. However, when the daily average counts were compared between the period 1 control group and the period 3 treatment group that included the same six animals, an overall reduction of 1.1 log10 CFU/g was observed, with a maximum decrease of 1.8 log10 CFU/g at day 21 (overall statistical significance, P = 0.001). Serotype O157:H7 was detected in 44% of the treatment group's intestinal tissue samples and in 64% of those from the control group (P < 0.04). These results indicated that the daily addition of 108 CFU of colicin E7-producing E. coli per gram of feed could reduce the fecal shedding of serotype O157:H7.

scholarly journals Associations of SNP rs_7927894 of FLG gene and TARC/CCL17 with atopic dermatitis in children

2021 ◽  
pp. 12-18
Author(s):  
V.O. Dityatkovsky ◽  
◽  
O.E. Abaturov ◽  
N.V. Naumenko ◽  
O.O. Alifirenko ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Statistical Significance ◽  
Venous Blood ◽  
Clinical Signs ◽  
Main Group ◽  
Control Group ◽  
Serum Concentrations ◽  
Control Groups ◽  
And Control ◽  
Severity Degree

One of the main genetic factors of the development of atopic dermatitis (AD) in children are single nucleotide polymorphisms (SNP) of the filagrin gene (FLG), particularly rs_7927894 FLG. One of the mostly studied and promising AD marker chemokines (CK) is the thymusE and activation regulated chemokine (TARC/CCL17). Purpose – to detect the associations and role of different variants of SNP rs_7927894 FLG gene and TARC/CCL17 in children suffering different AD clinical proE files (CP) – isolated or combined with comorbid atopic disorders (AtD). Materials and methods. The main group comprised 39 patients aged 3 to 18 years, suffering the isolated AD or combined with comorbid AtD. The control group comprised 47 patients aged 3 to 18 years, suffering the pathology of gastrointestinal tract without clinical signs of atopy. All the patients of the main and control groups had undergone detection of the genotype variants of SNP rs_7927894 FLG gene by real-time polymerase chain reaction and detection of TARC/CCL17 serum concentrations in venous blood. The cutEoff value of statistical significance was set as p<0.05. Results. The incidence and association of genotype variants C/C, C/T and T/T SNP rs_7927894 FLG gene in patients of cohorts of the studied groups were detected as follows: C/T rs_7927894 FLG was significantly the most common in the general main group (56.4%, p<0.05), within the cohort of CP AD isolated (61.1%, p<0.05) and CP of AD combined with comorbid AtD (52.4%, p<0.05). There were detected the associations of studied SNP with AD: C/T rs_7927894 FLG is significantly directly associated with AD (r=0.291, p<0.05), C/C rs_7927894 FLG has a reverse association with a trend to significance (r=-0.194, p=0.07). Mean serum concentrations of TARC/CCL17 did not differ significantly among patients cohorts of the main and control groups, respectively: general main group — 615.8 pg/ml, main with a CP AD isolated — 651.3 pg/ml, main with a CP of AD combined with comorbid AtD — 585.4 pg/ml, control — 608.4 pg/ml (p>0.05). Associations of serum TARC/CCL17 concentrations were determined as follows: elevation trending to significance within increasing AD severity degree (r=0.290, p=0.07) and significant elevation within the AD exhacerbation period (r=0.426, p<0.05). No significant association of TARC/CCL17 as to AD patients compared to the control group was detected in our study (r=-0.027, p>0.05). Conclusions. The genotype heterozygote variant C/T rs_7927894 FLG is significantly the most common and associated with all AD CP in children — isolated and combined with comorbid AtD. Variant C/C rs_7927894 of FLG gene is significantly reversely associated with AD in children. Serum concentrations of TARC/CCL17 did not reveal any significant differences between the AD patients and nonEatopic ones. However, they significantly elevate within AD exacerbation phase and trending to significance within AD severity degree increase in children. The research was carried out in accordance with the principles of the Helsinki declaration. The study protocol was approved by the Local Ethics Committee of all participating institutions. The informed consent of the patient was obtained for conducting the studies. No conflict of interest was declared by the authors. Key words: atopic dermatitis, children, associations, polymorphism, filaggrin, thymus- and activation regulated chemokine.

scholarly journals Axial length of the eye in the first three years of life in children with congenital cataract in one or both eyes

2008 ◽  
Vol 136 (1-2) ◽  
pp. 7-11
Author(s):  
Ivan Marjanovic ◽  
Ivan Stefanovic ◽  
Gordana Vlajkovic
Keyword(s):  
General Anesthesia ◽  
Axial Length ◽  
Congenital Cataract ◽  
Statistical Significance ◽  
Posterior Segment ◽  
Eye Disease ◽  
Control Group ◽  
Control Groups ◽  
And Control ◽  
Axial Growth

INTRODUCTION Ultrasound has an important role in the diagnostics of the posterior segment of the eye, when clinical examination is not possible. One of problems is congenital cataract. Using ultrasound biometry it is possible to measure the axial length of the eye in all ages and axial growth from the very beginning to its termination. OBJECTIVE To ratify growth of the infant?s eye with and without congenital cataract, and to determine the accuracy of ultrasound measuring with and without general anesthesia. METHOD In our study we analyzed 30 patients (48 eyes) who had congenital cataract in one or both eyes (observed group) and 20 patients (40 eyes) in whom no eye disease was diagnosed (control group). RESULTS The observed group of 30 patients (48 eyes) with congenital cataract was divided according to age into three subgroups: I) 4-12 months, 11 patients, 18 eyes; II) 13-24 months, 7 patients, 11 eyes; III) 25-36 months, 12 patients, 19 eyes. In control group we analyzed 20 patients (40 eyes) without congenital cataract. These patients were also divided, according to age, into three subgroups: I) 2-12 months, 5 patients, 10 eyes; II) 13-24 months, 6 patients, 12 eyes; III) 25-36 months, 9 patients, 18 eyes. CONCLUSION Comparing the average axial lengths of the eye in the first, second and third year of life between the observed and control groups, we did not find any statistical significance in the obtained values. When determining the accuracy of the ultrasound measuring of the axial length of the eye in the observed group, we found no statistical significance between the determined values weather the measurements were done with or without general anesthesia (p>0.05).

scholarly journals Impact of Ileal Transposition Surgical Intervention on Antioxidant Status Measured in Liver Tissue of Obese Zucker Rats (Crl:ZUC-Leprfa)

2018 ◽  
Vol 2018 ◽  
pp. 1-8 ◽  
Author(s):  
Bronisława Skrzep-Poloczek ◽  
Dominika Stygar ◽  
Tomasz Sawczyn ◽  
Ewa Romuk ◽  
Elżbieta Chełmecka ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Liver Tissue ◽  
Antioxidant Status ◽  
Zucker Rats ◽  
Control Group ◽  
Ileal Transposition ◽  
Control Groups ◽  
Sham Procedure ◽  
Total Antioxidant ◽  
And Control

Background. The main factor characteristic for low-grade systemic inflammation typical for obesity is oxidative stress (OS). Reactive oxygen species (ROS) production is higher and more increased in time in the obese patients than in lean subjects. Aims. To assess the effect of ileal transposition (IT) and sham types of bariatric procedures on the antioxidative systems in the liver tissue of Zucker rats (Crl:ZUC Leprfa). Method. 21 animals were divided into the experimental groups: control group (n=7), sham group (n=7), and IT group (n=7). Sham and IT animals underwent selected surgery. The concentration of total antioxidant capacity (TAC), total antioxidant status (TOS), and activity of glutathione reductase (glutathione-disulfide reductase, GR, GSR), catalase (CAT), glutathione peroxidase (GPx), glutathione S-transferase (GST), and total superoxide dismutase activity (SOD) were assessed in liver tissue 3 months after surgery. Results. IT procedure significantly increased TAC when compared to sham and the control group. Animals after IT showed higher levels of TOS when compared to sham procedure. The total amount of TOS was similar in IT and control groups. GPx activity was increased in the groups submitted to the sham and IT surgery in relation to control. GR and CAT activities were significantly higher after IT in comparison to control and sham procedures. Total SOD and MnSOD were significantly higher in sham-operated animals in comparison to IT intervention and control groups. Conclusions. IT procedure had a positive impact on the diminishing of oxidative stress measured by TAC and TOS markers. The dynamic, adaptive, and protective mechanisms of enzymatic antioxidant systems were observed after the IT but not sham procedure. Nevertheless, 3 months after surgery, the midterm effect of bariatric surgery was observed, which might not fully balance the antioxidative response.

scholarly journals Effect of integrated health system leading-managing-and-governing for results model on institutional delivery: Team-based quasi-experiment

2019 ◽  
Author(s):  
Yeshambel Agumas Ambelie ◽  
Getu Degu Alene ◽  
Damen Hailemariam Gebrekiros
Keyword(s):  
Institutional Delivery ◽  
Statistical Significance ◽  
Intervention Group ◽  
Analysis Of Covariance ◽  
Theory Of Change ◽  
Control Group ◽  
Control Groups ◽  
Institutional Deliveries ◽  
Quasi Experimental ◽  
And Control

AbstractObjectiveThe objective of this study is to examine, based on theory of change, whether integrated leading-managing-and-governing for results model is plausible cause of improved institutional delivery.MethodsA team-based quasi-experimental study was conducted. One-hundred-thirty-four health facility teams were enrolled in the study. Teams were allocated to intervention and control groups in a 1:1 ratio, non-randomly. End line institutional delivery was the dependent variable while the group (main predictor) and the baseline institutional delivery (covariate) were independent variables. The intervention that was given over six months was integrated leading-managing-and-governing for results model. The institutional deliveries were measured with percentages whilst the group was measured with exposure status (yes or no) to the intervention. Data, from both groups, were collected at baseline and end line. Data were analyzed using analysis of covariance. Statistical significance was determined at (p<.05). The main effect of the intervention was determined by 95% CI, presented in the contrast results.ResultsThe adjusted mean institutional deliveries with 95% CI were 47.4 (46.2, 48.6) and 33.4 (32.2, 34.6) in the intervention and control groups, respectively. Contrast results showed that having an intervention group, p = .000, 95% CI (12.2, 15.8), of integrated leading-managing-and governing for results model significantly increased mean institutional delivery compared to having a control group.ConclusionsThis study provides some guidance regarding the plausible causation of integrated leading-managing-and-governing for results model on institutional delivery. It would serve as a baseline in identifying true causation using a randomized design.

scholarly journals Effect of cryotherapy in preventing mucositis associated with the use of 5-fluorouracil

2020 ◽  
Vol 28 ◽  
Author(s):  
Andrea Bezerra Rodrigues ◽  
Maria Isis Freire De Aguiar ◽  
Patrícia Peres De Oliveira ◽  
Naiana Pacifico Alves ◽  
Renan Alves Silva ◽  
...  
Keyword(s):  
Statistical Significance ◽  
Antineoplastic Agent ◽  
Control Group ◽  
Control Groups ◽  
Double Blind ◽  
Physiological Serum ◽  
Significant Difference ◽  
Oral Cryotherapy ◽  
And Control ◽  
Experimental Group

Objective: to evaluate the effect of oral cryotherapy compared to physiological serum on the development of oral mucositis in outpatient cancer patients using the 5-fluorouracil antineoplastic agent. Method: this is a controlled, randomized, double-blind, and multi-center clinical trial, conducted with 60 patients undergoing chemotherapy. The experimental group (n=30) used oral cryotherapy during the infusion of the 5-FU antineoplastic agent, while the control group (n=30) performed mouthwash with physiological serum at their homes. The oral cavity of the participants was assessed at three times: before randomization, and on the 7th and 14th days after using 5-FU. For data analysis, descriptive analyses and the ANOVA, paired t, and McNemar tests were used. Results: there was no statistically significant difference between the experimental and control groups in the assessments regarding the grade of mucositis. However, cryotherapy presented the chance to reduce the presence of intragroup mucositis, between the first and second assessments (p=0.000126). Conclusion: cryotherapy did not obtain statistical significance in relation to oral hygiene with serum, but it proved to be effective intragroup. Record number: RBR-4k7zh3

EVALUATION OF THE TREATMENT EFFECTS OF SKINTACT-ELECTRO COMBINED WITH "DAI TAN GIAO THANG" REMEDY IN THE TREATMENT OF PERIPHERALLY FACIAL PARALYSIS CAUSED BY COLD

2012 ◽  
pp. 70-75
Author(s):  
Thi Dieu Hang Le ◽  
Thi Tan Nguyen ◽  
Duc Dung Hoang
Keyword(s):  
Facial Paralysis ◽  
Treatment Effects ◽  
Treatment Time ◽  
Statistical Significance ◽  
Study Groups ◽  
Control Group ◽  
Study Group ◽  
Control Groups ◽  
Average Treatment ◽  
And Control

Objectives: Evaluation of the treatment effects of the skintact-electrode combined with "Dai tan giao thang" remedy and compared with skintact- electrode. Methods: 38 patients, diagnosed peripherally facial paralysis caused by cold, were examined and treated at Thua Thien Hue traditional medicine hospital, were divided into 2 groups: the study group: skintact-electrode combined with remedy, the control group: skintact-electrode only.This study was a randomizedly controlled clinical trial, comparison and control. Results: Occur at any age and gender, mostly in labor age making up 52.63%. After treatment, the botanical disorder and movement disorder symptoms at study groups reduced much more than that at control groups. The results of the good and reduced treatment of the study group are higher than that of the control group and the difference between 2 groups has statistical significance. The sooner patients were treated, the higher rate of recovering illness was, the recovering rate of study group and control group is 53.57% (less than 7 days); 42.86% (less than 7 days), respectively. Average treatment time: study group: 21.5 ± 6.1; control group: 25.6 ± 9.34 days. Conclusions: The treatment of peripherally facial paralysis caused by cold with the skintact-electrode combined with "Dai tan giao thang" remedy showed that the completely recovering rate of study group is 89.47% and the average treatment time is 21.5 ± 6.1 days. The good and fairly results of the study groups is higher than the control groups (p<0.05).

Conjugated Linoleic Acid Causes a Marked Increase in Liver α-Tocopherol and Liver α-Tocopherol Transfer Protein in C57BL/6 J Mice

2010 ◽  
Vol 80 (1) ◽  
pp. 65-73 ◽  
Author(s):  
Pei-Min Chao ◽  
Wan-Hsuan Chen ◽  
Chun-Huei Liao ◽  
Huey-Mei Shaw
Keyword(s):  
Antioxidant Enzymes ◽  
Linoleic Acid ◽  
Conjugated Linoleic Acid ◽  
Thiobarbituric Acid ◽  
Control Group ◽  
Thiobarbituric Acid Reactive Substances ◽  
Control Groups ◽  
Protein Levels ◽  
Transfer Protein ◽  
And Control

Conjugated linoleic acid (CLA) is a collective term for the positional and geometric isomers of a conjugated diene of linoleic acid (C18:2, n-6). The aims of the present study were to evaluate whether levels of hepatic α-tocopherol, α-tocopherol transfer protein (α-TTP), and antioxidant enzymes in mice were affected by a CLA-supplemented diet. C57BL/6 J mice were divided into the CLA and control groups, which were fed, respectively, a 5 % fat diet with or without 1 g/100 g of CLA (1:1 mixture of cis-9, trans-11 and trans-10, cis-12) for four weeks. α-Tocopherol levels in plasma and liver were significantly higher in the CLA group than in the control group. Liver α-TTP levels were also significantly increased in the CLA group, the α-TTP/β-actin ratio being 2.5-fold higher than that in control mice (p<0.01). Thiobarbituric acid-reactive substances were significantly decreased in the CLA group (p<0.01). There were no significant differences between the two groups in levels of three antioxidant enzymes (superoxide dismutase, glutathione peroxidase, and catalase). The accumulation of liver α-tocopherol seen with the CLA diet can be attributed to the antioxidant potential of CLA and the ability of α-TTP induction. The lack of changes in antioxidant enzyme protein levels and the reduced lipid peroxidation in the liver of CLA mice are due to α-tocopherol accumulation.

Sri Lankan Cleft Lip and Palate Study Model Analysis: Clefts of the Secondary Palate

1993 ◽  
Vol 30 (2) ◽  
pp. 227-230 ◽  
Author(s):  
Andrew Mccance ◽  
David Roberts-Harry ◽  
Martyn Sherriff ◽  
Michael Mars ◽  
William J.B. Houston
Keyword(s):  
Cleft Lip ◽  
Cleft Lip And Palate ◽  
Model Analysis ◽  
Control Group ◽  
Tooth Size ◽  
Sri Lankan ◽  
Control Groups ◽  
Secondary Palate ◽  
Comparable Control ◽  
And Control

The study models of a group of adult Sri Lankan patients with clefts of the secondary palate were investigated. Tooth-size and arch-dimension comparisons were made with a comparable control group. Significant differences were found between the cleft and control groups in tooth sizes, chord lengths, and arch widths. The cleft group dimensions were generally smaller than those of the control group. Overjets were larger in the cleft group.

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