Evaluation of Association Between Q192R and L55M Genetic Polymorphisms of PON1 and Serum Paraoxonase-1 Activity in Healthy Individuals, a Meta-Analysis

2018 ◽  
Vol 25 (2) ◽  
pp. 171-180 ◽  
Author(s):  
Mostafa Saadat
Keyword(s):  
Meta Analysis ◽  
Activity Level ◽  
Paraoxonase 1 ◽  
Coding Region ◽  
Paraoxonase Activity ◽  
Variant Alleles ◽  
Pon1 Polymorphisms ◽  
Serum Paraoxonase ◽  
The Relationship ◽  
Healthy Participants

Abstract Background and Aims: Several studies have reported the alteration of the paraoxonase 1 (PON1) enzyme activity in various diseases, including diabetes mellitus. The Q192R and L55M are two genetic variations in the coding region of PON1. To evaluate the relationship between these polymorphisms and the alteration in serum paraoxonase activity, the present meta-analysis was carried out. Material and Methods: Eligible studies published before October 2017 was identified in several databases. The paraoxonase activity in subjects with variant alleles of the study polymorphisms were normalized using the activity of the QQ or LL genotypes. The pooled mean effect of alterations in activity level and its 95% confidence intervals (95% CI) was calculated. Results: Thirty-two studies including 11532 healthy participants were used for the present meta-analysis. The paraoxonase activity was increased in the QR and RR genotypes. This elevation was greater among Caucasians than those among Asians and Africans. The activity in the LM and MM genotypes compared with the LL genotype were decreased, this reduction in Caucasians was greater than Africans. Conclusions: At least in part other PON1 polymorphisms and environmental factors may accounts for heterogeneity between studies.

Relationship between total plasma homocysteine and the risk of aneurysms – a meta-analysis

VASA ◽  
2020 ◽  
pp. 1-6
Author(s):  
Hanji Zhang ◽  
Dexin Yin ◽  
Yue Zhao ◽  
Yezhou Li ◽  
Dejiang Yao ◽  
...  
Keyword(s):  
Large Scale ◽  
Meta Analysis ◽  
Single Species ◽  
Total Plasma ◽  
Control Groups ◽  
Randomized Controlled ◽  
Randomized Controlled Studies ◽  
The Difference ◽  
The Relationship ◽  
Healthy Participants

Summary: Our meta-analysis focused on the relationship between homocysteine (Hcy) level and the incidence of aneurysms and looked at the relationship between smoking, hypertension and aneurysms. A systematic literature search of Pubmed, Web of Science, and Embase databases (up to March 31, 2020) resulted in the identification of 19 studies, including 2,629 aneurysm patients and 6,497 healthy participants. Combined analysis of the included studies showed that number of smoking, hypertension and hyperhomocysteinemia (HHcy) in aneurysm patients was higher than that in the control groups, and the total plasma Hcy level in aneurysm patients was also higher. These findings suggest that smoking, hypertension and HHcy may be risk factors for the development and progression of aneurysms. Although the heterogeneity of meta-analysis was significant, it was found that the heterogeneity might come from the difference between race and disease species through subgroup analysis. Large-scale randomized controlled studies of single species and single disease species are needed in the future to supplement the accuracy of the results.

scholarly journals Paraoxonase 1 and Non-Alcoholic Fatty Liver Disease: A Meta-Analysis

Molecules ◽  
2021 ◽  
Vol 26 (8) ◽  
pp. 2323
Author(s):  
Kazuhiko Kotani ◽  
Jun Watanabe ◽  
Kouichi Miura ◽  
Alejandro Gugliucci
Keyword(s):  
Oxidative Stress ◽  
Liver Disease ◽  
Fatty Liver ◽  
Fatty Liver Disease ◽  
Meta Analysis ◽  
Laboratory Data ◽  
Paraoxonase 1 ◽  
Mixed Data ◽  
Alcoholic Fatty Liver ◽  
Paraoxonase Activity

Oxidative stress is involved in the pathophysiology of nonalcoholic fatty liver disease (NAFLD). However, reliable biomarkers of NAFLD in relation to oxidative stress are not available. While paraoxonase 1 (PON1) is an antioxidant biomarker, there appears to be mixed data on PON-1 in patients with NAFLD. The aim of this meta-analysis was to assess the current data on PON1 activity (i.e., paraoxonase and arylesterase) in patients with NAFLD. A PubMed, CENTRAL, and Embase search identified 12 eligible articles. In the meta-analysis, the paraoxonase activity was low in patients with NAFLD (mean difference (MD) −27.17 U/L; 95% confidence interval (CI) −37.31 to −17.03). No difference was noted in the arylesterase activity (MD 2.45 U/L; 95% CI −39.83 to 44.74). In a subgroup analysis, the paraoxonase activity was low in biopsy-proven nonalcoholic steatohepatitis (MD −92.11 U/L; 95% CI −115.11 to −69.11), while the activity in NAFLD as diagnosed by ultrasonography or laboratory data was similar (MD −2.91 U/L; 95% CI −11.63 to 5.80) to that of non-NAFLD. In summary, the PON1, especially paraoxonase, activity could be a useful biomarker of NAFLD. Further studies are warranted to ascertain the relevance of PON1 measurements in patients with NAFLD.

scholarly journals Paraoxonase 1 and Chronic Obstructive Pulmonary Disease: A Meta-Analysis

Antioxidants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 1891
Author(s):  
Jun Watanabe ◽  
Kazuhiko Kotani ◽  
Alejandro Gugliucci
Keyword(s):  
Chronic Obstructive Pulmonary Disease ◽  
Pulmonary Disease ◽  
Meta Analysis ◽  
Paraoxonase 1 ◽  
Pon1 Activity ◽  
Chronic Obstructive ◽  
Healthy Controls ◽  
Obstructive Pulmonary Disease ◽  
Paraoxonase Activity ◽  
Severe Copd

Oxidative stress is a driving factor in the pathophysiology of chronic obstructive pulmonary disease (COPD). While paraoxonase 1 (PON1) is an antioxidant enzyme and a potential biomarker of this disease, data regarding the status of PON-1 in COPD are inconclusive. In this regard, to shed light on this issue, we performed a meta-analysis of data on PON1 activity in COPD. Electronic databases (MEDLINE, Embase and CENTRAL) were searched for available studies on PON1 activity in patients with stable COPD published before October 2021. A meta-analysis was performed using random-effects models. Twelve studies (12 studies on paraoxonase and three on arylesterase) were identified. Patients with COPD had lower levels of paraoxonase activity (standard mean difference [SMD] −0.77, 95% confidence interval [CI] −1.35 to −0.18) and arylesterase activity (SMD −1.15, 95% CI −1.95 to −0.36) in comparison to healthy controls. In subgroup analyses, paraoxonase activity was lower in patients of studies as consisted of mainly non-severe COPD (SMD −1.42, 95% CI −2.04 to −0.79) and, by contrast, slightly higher in patients of studies including severe COPD (SMD 0.33, 95% CI 0.02 to 0.64) in comparison to healthy controls. Arylesterase activity showed a similar trend. Overall, PON1 activity was lower in patients with COPD, suggesting that PON1-related antioxidant defense is impaired in COPD. Future studies are warranted.

A meta-analysis of the relationship between paraoxonase 1 polymorphisms and cancer

2019 ◽  
Vol 53 (11-12) ◽  
pp. 1045-1050 ◽  
Author(s):  
Ping Liu ◽  
Qishun Wang ◽  
Yongsheng Cui ◽  
Jian Wang
Keyword(s):  
Meta Analysis ◽  
Paraoxonase 1 ◽  
The Relationship

Serum paraoxonase activity and oxidative stress levels in patients with cutaneous anthrax

2016 ◽  
Vol 36 (7) ◽  
pp. 663-669
Author(s):  
S Karadas ◽  
M Aslan ◽  
MR Ceylan ◽  
M Sunnetcioglu ◽  
N Bozan ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Paraoxonase 1 ◽  
Pon1 Activity ◽  
Bacterial Disease ◽  
Healthy Controls ◽  
Paraoxonase Activity ◽  
Cutaneous Anthrax ◽  
Bacterium Bacillus ◽  
Serum Paraoxonase ◽  
And Oxidative Stress

Introduction: Anthrax is a bacterial disease caused by the aerobic sporeforming bacterium Bacillus anthracis. It has been suggested that oxidative stress plays an important role in the pathogenesis of B. anthracis. The aim of this study was to investigate serum paraoxonase 1 (PON1) activity, catalase activity, malondialdehyde (MDA) levels, and superoxide dismutase (SOD) levels in patients with cutaneous anthrax. Materials and methods: Fifteen patients with cutaneous anthrax and 15 healthy controls were enrolled in this study. The serum MDA levels, SOD levels, paraoxonase, arylesterase, and catalase activities were measured using a spectrophotometer. Results: The serum SOD levels, paraoxonase, arylesterase, and catalase activities were significantly lower in patients with cutaneous anthrax than in controls (for all, p < 0.001), whereas MDA levels were significantly higher ( p < 0.001). No significant correlation was found between serum paraoxonase activity, arylesterase activity, SOD levels, and MDA levels (all, p > 0.05) in patients with cutaneous anthrax. Conclusions: The current study was the first to show decreased antioxidant levels and increased oxidant levels in patients with cutaneous anthrax. Therefore, decreased PON1 activity may play a role in the pathogenesis of cutaneous anthrax.

scholarly journals Distribution of paraoxonase 1 coding region polymorphisms in Serbian population

Genetika ◽  
2010 ◽  
Vol 42 (2) ◽  
pp. 235-247 ◽  
Author(s):  
I. Pejin-Grubisa ◽  
I. Buzadzic ◽  
B. Jankovic-Orescanin ◽  
N. Barjaktarovic-Vucinic
Keyword(s):  
Catalytic Activity ◽  
High Density Lipoprotein ◽  
Organophosphorus Pesticides ◽  
Density Lipoprotein ◽  
Paraoxonase 1 ◽  
Coding Region ◽  
Pon1 Gene ◽  
Toxic Metabolites ◽  
Gene Coding ◽  
Serum Paraoxonase

Serum paraoxonase 1 (PON1) in humans is a protein component of high-density lipoprotein (HDL) particles that protects against oxidative damage, detoxifies toxic metabolites of organophosphorus pesticides and nerve agents and activates or inactivates specific drugs. It has been reported that PON1 gene coding region polymorphisms, L55M and Q192R, could influence both expression level and catalytic activity of PON1, and their link with a broad spectrum of diseases has been described. The aim of this study was to determine the frequencies of PON1 coding region polymorphisms Q192R and L55M in Serbian population. The most frequent alleles were Q (0.77) for Q192R and L (0.68) for L55M. Genotypes QQ (0.60) and LL (0.47) and combined genotype QQ/LL (0.26) were the most frequent in examined population.

scholarly journals Influence of PON1 Polymorphisms on the Association between Serum Paraoxonase 1 and Homocysteinemia in a General Population

2006 ◽  
Vol 52 (4) ◽  
pp. 781-782 ◽  
Author(s):  
Michelle M Murphy ◽  
Judit Marsillach ◽  
Jordi Camps ◽  
Joan Fernández-Ballart ◽  
Bharti Mackness ◽  
...  
Keyword(s):  
General Population ◽  
Paraoxonase 1 ◽  
Pon1 Polymorphisms ◽  
Serum Paraoxonase

Effect of organophosphate intoxication on human serum paraoxonase

2002 ◽  
Vol 21 (5) ◽  
pp. 247-252 ◽  
Author(s):  
E Y Sözmen ◽  
B Mackness ◽  
B Sözmen ◽  
P Durrington ◽  
F K Girgin ◽  
...  
Keyword(s):  
Risk Factors ◽  
Healthy Volunteers ◽  
Metabolic Activity ◽  
Clinical Manifestations ◽  
Pon1 Activity ◽  
Coding Region ◽  
Interindividual Differences ◽  
Interindividual Variations ◽  
Pon1 Polymorphisms ◽  
Serum Paraoxonase

Recently, interindividual variations in serum paraoxonase (PON1) activity and the differences in its metabolic activity towards different organophosphates (OPs) caused by the coding region polymorphisms L55M and Q192R have been found to be important risk factors in susceptibility to OP poisoning. In this study, we investigated the effect of PON1 on the outcome of acute OP intoxication and the effect of acute OP intoxication on PON1. Twenty-eight OP-poisoned patients and 66 healthy volunteers were studied. Patients were evaluated for the clinical manifestations of OP intoxication as well as PON1 activity, PON1 mass and PON1 polymorphisms. Butyrylcholine-esterase (BChE) activity was 50% lower (2276&plusmn;738 U/L versus 5037&plusmn;1553 U/L, P&lt;0.01) while PON1 activity was 30% lower (114.2&plusmn;67.4 nmol/mL/min versus 152.9&plusmn;78.9 nmol/mL/ min, P&lt;0.05) in patients than in controls. We observed that the PON1 and BChE activities of eight of the original subjects returned to normal levels when they were reinvestigated six months after exposure. The frequency of the PON192Q allele was significantly higher in patients than controls (85.7% versus 59.7%, &chi;2=6.745, P=0.034). QQ/MM individuals had the lowest activity towards paraoxon, while RR/LL individuals had the highest activity. Our data indicate that interindividual differences in PON1 activity and the PON1-55 and-192 polymorphisms are important risk factors in susceptibility to acute OP poisoning; therefore, identifying an individual's PON1 alloenzymes may play an important role in the treatment of patients suffering from OP intoxication.

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